Kl (NM_031336) Rat Tagged ORF Clone
CAT#: RR213144
- TrueORF®
Kl (Myc-DDK-tagged ORF) - Rat Klotho (Kl), (10 ug)
"NM_031336" in other vectors (5)
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CNY 10,592.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR213144 representing NM_031336
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCCCGCGCCCCTCCTCGCCGCCTGCCGCGGCTCTTGCTGCTCCGTTTGCTGTCGCTGCATCTGC TGTTGCTCACCCTGCGCGCCCGCTGCCTAAGCGCTGAGCCGGGTCAGGGCGCGCAGACCTGGGCTCGCTT TGCGCGCCCTCCTGTCCCAGAGGCCTCTGGCCTCCTCCATGACACCTTCCCCGATGGTTTCCTCTGGGCG GTAGGCAGCGCCGCCTACCAGACGGAGGGCGGCTGGCGACAGCACGGCAAAGGCGCGTCCATCTGGGACA CTTTCACCCATCACCCTCGGGCCATCCCGGAGGACTCCCCGATCGTCATGGCGCCGTCGGGTGCCCCGTT GCCTCCCCTGCCCTCCACTGGAGATGTGGCCAGCGACAGTTACAACAACGTCTACCGCGACACAGAGGGG CTACGCGAACTGGGGGTCACCCACTACCGCTTCTCCATATCGTGGGCGCGGGTGCTCCCCAATGGCACCG CGGGCACCCCCAACCGCGAGGGGCTGCGCTACTACCGGCGGCTGCTGGAGCGGCTGAGGGAGCTGGGCGT GCAGCCGGTGGTCACTCTGTACCATTGGGACCTGCCGCAGCGCCTGCAGGACACCTATGGCGGCTGGGCC AATCGCGCCCTGGCCGACCATTTCAGGGATTACGCCGAGCTCTGCTTCCGCCACTTCGGTGGTCAGGTCA AGTACTGGATCACCATTGACAACCCCTACGTGGTGGCCTGGCACGGGTACGCCACCGGCCGCCTGGCCCC GGGCGTGCGGGGCAGCTCCAGGCTCGGGTACCTGGTTGCCCACAACCTACTTTTGGCTCACGCCAAAGTC TGGCGTCTCTACAACACCTCCTTCCGCCCCACTCAGGGGGGCCGGGTGTCCATCGCCTTAGGTTCCCATT GGATCACCCCTCGAAGGATGACCGACTATCACATCAGAGAGTGTCAGAAGTCTCTTGACTTCGTGCTAGG CTGGTTTGCCAAGCCCATATTTATCGATGGCGACTACCCAAAGAGTATGAAGAACAACCTCTCATCCCTG CTGCCCGATTTTACTGAATCCGAGAAGAGATTCATCAGGGGAACAGCTGACTTCTTTGCTCTCTCCTTCG GACCAACCTTGAGCTTTCAACTACTGGACCCCAGCATGAAGTTCCGCCAGCTGGAGTCTCCCAGCCTGAG GCAGCTTCTGTCTTGGATAGATCTGGAGTATAACCACCCTCAGATATTTATTGTAGAAAATGGCTGGTTT GTCTCGGGGACCACCAGAAGAGATGATGCCAAATACATGTATTATCTCAAGAAGTTCATAATGGAAAGCT TAAAAGCCATCAGGCTGGATGGGGTCGACGTCATTGGGTACACCGCATGGTCCCTCATGGATGGTTTCGA GTGGCACAGGGGCTACAGCATCCGACGAGGACTCTTCTATGTCGACTTTCTGAGTCAGGACAAGGAGTTG TTGCCCAAGTCTTCGGCCTTGTTCTACCAAAAGCTGATAGAGAACAATGGCTTCCCTCCTTTACCTGAGA ACCAGCCCCTTGAAGGGACGTTTCCCTGTGACTTTGCTTGGGGAGTGGTTGACAACTACATTCAAGTGGA CCCCACTCTCTCTCAGTTTACTGACCCGAATGTCTATCTGTGGGATGTGCATCACAGTAAGAGGCTTATT AAAGTAGACGGGGTTGTAGCCAAGAAGAGAAAACCTTACTGTGTTGATTTCTCTGCCATCCGGCCTCAGA TAACCTTACTTCGAGAAATGCGGGTCACCCACTTTCGGTTCTCCCTAGACTGGGCCCTGATCTTGCCTCT GGGTAACCAGACTCAAGTGAACCGCACGGTTCTGCACTTCTACCGCTGTATGGTCAGCGAGCTGGTGCAC GCCAACATCACTCCAGTGGTGGCCCTGTGGCAACCAGCAACCCCACACCAAGGCCTGCCACATGCCCTTG CAAAACATGGGGCCTGGGAGAACCCGCACACTGCCCTGGCATTTGCAGACTACGCAAACCTGTGCTTTGA AGAGCTAGGCCACTGGGTCAAGTTCTGGATCACCATAAATGAGCCAAACTCACGGAACATGACATACCGT GCGGGGCACCACCTGCTTAAGGCTCATGCCTTGGCATGGCACCTGTACGATGACAAATTTAGGGCGGCTC AGAAAGGCAAAATATCCATCGCCTTGCAGGTTGACTGGATAGAACCGGCCTGCCCATTCTCTCAGAAGGA CAAAGAAGTGGCGGAGAGAGTTTTGGAATTTGATGTAGGCTGGCTGGCAGAGCCCATTTTCGGCTCCGGA GATTATCCGCATGTGATGAGGGAATGGCTGAACCAAAAAAACAACTTTCTTCTGCCCTATTTCACGGAAG ATGAGAAAAAGCTAATCCGGGGTTCCTTTGACTTCCTGGCGTTGAGCCATTACACCACCATCCTTGTAGA CTGGGAAAAGGAGGATCCGATAAAATACAACGATTACCTGGAGGTGCAGGAGATGACTGACATCACGTGG CTCAACTCCCCCAATCAGGTGGCGGTGGTGCCTTGGGGGCTGCGCAAAGCGCTCAACTGGCTAAGGTTCA AGTATGGAGACCTCCCGATGTTCGTGACAGCCAATGGCATTGATGACGATCCCCATGCCGAGCAAGACTC ACTGAGGATGTATTATATTAAGAATTACGTGAATGAGGCTCTGAAAGCCTACGTGTTGGACGGCATCAAC CTTTGTGGCTACTTTGCGTATTCGCTTAGTGACCGCTCAGTTCCCAAGTCCGGCTTTTATCGATACGCTG CGAATCAGTTCGAGCCCAAACCGTCTATTAAACATTACAGGAAAATTATTGACAACAACGGCTTCCTGGG TTCTGGAACGCTGGGAAGGTTTTGTCCGGAAGAGTACACCGTGTGCACCGGATGCGGCTTTTTTCAAACC CGGAAGTCCCTGCTGGCCTTCATCTCGTTTCTAGTTTTTGCTTTCGTTACTTCTCTCGCTCTTATTTATT ACTACTCCAAGAAAGGCCGGAGACGTTATAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213144 representing NM_031336
Red=Cloning site Green=Tags(s) MPARAPPRRLPRLLLLRLLSLHLLLLTLRARCLSAEPGQGAQTWARFARPPVPEASGLLHDTFPDGFLWA VGSAAYQTEGGWRQHGKGASIWDTFTHHPRAIPEDSPIVMAPSGAPLPPLPSTGDVASDSYNNVYRDTEG LRELGVTHYRFSISWARVLPNGTAGTPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDTYGGWA NRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGVRGSSRLGYLVAHNLLLAHAKV WRLYNTSFRPTQGGRVSIALGSHWITPRRMTDYHIRECQKSLDFVLGWFAKPIFIDGDYPKSMKNNLSSL LPDFTESEKRFIRGTADFFALSFGPTLSFQLLDPSMKFRQLESPSLRQLLSWIDLEYNHPQIFIVENGWF VSGTTRRDDAKYMYYLKKFIMESLKAIRLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKEL LPKSSALFYQKLIENNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVDPTLSQFTDPNVYLWDVHHSKRLI KVDGVVAKKRKPYCVDFSAIRPQITLLREMRVTHFRFSLDWALILPLGNQTQVNRTVLHFYRCMVSELVH ANITPVVALWQPATPHQGLPHALAKHGAWENPHTALAFADYANLCFEELGHWVKFWITINEPNSRNMTYR AGHHLLKAHALAWHLYDDKFRAAQKGKISIALQVDWIEPACPFSQKDKEVAERVLEFDVGWLAEPIFGSG DYPHVMREWLNQKNNFLLPYFTEDEKKLIRGSFDFLALSHYTTILVDWEKEDPIKYNDYLEVQEMTDITW LNSPNQVAVVPWGLRKALNWLRFKYGDLPMFVTANGIDDDPHAEQDSLRMYYIKNYVNEALKAYVLDGIN LCGYFAYSLSDRSVPKSGFYRYAANQFEPKPSIKHYRKIIDNNGFLGSGTLGRFCPEEYTVCTGCGFFQT RKSLLAFISFLVFAFVTSLALIYYYSKKGRRRYK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_031336 |
ORF Size | 3042 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031336.1, NP_112626.1 |
RefSeq Size | 3525 bp |
RefSeq ORF | 3045 bp |
Locus ID | 83504 |
UniProt ID | Q9Z2Y9 |
MW | 116.8 kDa |
Gene Summary | may be involved in suprresion of aging phenotypes [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN213144 | Kl (untagged ORF) - Rat Klotho (Kl), (10 ug) |
CNY 16,340.00 |
|
RR213144L1 | Lenti ORF clone of Kl (Myc-DDK-tagged ORF) - Rat Klotho (Kl), (10 ug) |
CNY 12,992.00 |
|
RR213144L2 | Lenti ORF clone of Kl (mGFP-tagged ORF) - Rat Klotho (Kl), (10 ug) |
CNY 19,670.00 |
|
RR213144L3 | Lenti ORF clone of Kl (Myc-DDK-tagged ORF) - Rat Klotho (Kl), (10 ug) |
CNY 17,860.00 |
|
RR213144L4 | Lenti ORF clone of Kl (mGFP-tagged ORF) - Rat Klotho (Kl), (10 ug) |
CNY 19,670.00 |