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Hsph1 (NM_001011901) Rat Tagged ORF Clone
CAT＃: RR213187
- TrueORF®
Hsph1 (Myc-DDK-tagged ORF) - Rat heat shock 105kDa/110kDa protein 1 (Hsph1), (10 ug)
"NM_001011901" in other vectors (3)
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CNY 10,930.00
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CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Hsp105 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213187 representing NM_001011901
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTGGTTGGGCTAGACGTGGGCTCGCAGAGCTGCTACATTGCAGTGGCACGGGCCGGGGGCATCG AGACCATCGCCAACGAGTTCAGCGACCGCTGCACCCCGTCAGTCATATCATTTGGACCAAAAAACAGAAC AATTGGAGTTGCAGCCAAAAACCAGCAAATCACTCATGCAAACAATACGGTGTCTAGCTTTAAGAGATTT CATGGCAGAGCATTCAATGATCCCTTCATTCAAAAGGAAAAGGAAAACTTGAGCTATGATTTGGTCCCAA TGAAGAATGGTGGTGTTGGAATAAAGGTCATGTACATGGACGAAGACCATCTCTTTAGCGTGGAGCAGAT AACAGCCATGCTGCTGACTAAGTTGAAGGAAACTGCGGAAAACAACCTCAAGAAGCCGGTGACAGACTGT GTCATCTCGGTCCCGTCCTTCTTCACAGATGCTGAGAGGAGGTCTGTTCTGGATGCCGCACAGATTGTGG GCTTGAACTGCTTGCGGCTCATGAATGACATGACGGCTGTTGCTTTGAATTATGGAATTTATAAGCAGGA TCTCCCGAATGCAGACGAGAAACCCCGAGTCGTGGTGTTTGTGGACATGGGACACTCCTCTTTCCAAGTG TCTGCCTGTGCTTTTAACAAAGGGAAACTGAAGGTTCTAGGCACAGCTTTTGATCCCTTCTTGGGAGGAA AGAACTTTGACGAGAAGCTCGTAGAGCATTTTTGTGCTGAATTTAAAACCAAGTACAAATTAGATGCAAA ATCCAAAATTCGAGCCCTCCTTCGTCTCCATCAGGAGTGTGAAAAGTTAAAGAAGCTCATGAGTTCTAAC AGCACAGACCTGCCGCTGAACATTGAGTGCTTTATGAATGACAAGGACGTCTCTGCAAAGATGAACAGGT CACAGTTTGAAGAACTGTGTGCTGAGCTCCTACAAAAAATAGAGGTCCCCCTTCACTTGTTGATGGAACA AACTCACCTCAAGACTGAAGAAGTGAGCGCCATTGAGATAGTTGGAGGAGCCACAAGAATCCCAGCTGTG AAGGAAAGAATTGCCAGATTCTTTGGGAAAGACGTCAGTACCACGCTCAACGCAGACGAGGCTGTGGCTA GAGGGTGCGCACTGCAGTGTGCAATTCTTTCTCCGGCATTTAAAGTTAGAGAGTTCTCCGTCACCGATGC AGTTCCTTTCCCAATATCTCTGGTCTGGAACCATGACTCAGAAGAAACTGAAGGTGTTCACGAGGTGTTC AGTCGGAACCATGCTGCTCCTTTCTCCAAAGTGCTCACCTTCCTGAGAAGGGGACCCTTTGAACTAGAAG CTTTCTATTCTGACCCTCAAGCAGTTCCATATCCAGAAGCAAAAATCGGCCGTTTTGTCGTTCAGAATGT TTCTGCACAGAAAGATGGAGAAAAATCTAAAGTGAAAGTCAAAGTGCGTGTGAACACACATGGCATTTTC ACCATATCCACGGCATCCATGGTGGAAAAAGTCCCGACTGAGGAGGAGGATGGGTCCTCTGTCGAGGCAG ACATGGAGTGTCCAAACCAGAAACCAGCAGAAAGCTCGGATGTGGATAAAAATATCCAGCAAGACAACAG TGAAGCTGGAACACAGCCCCAGGTACAAACTGATGGTCAACAAACCTCACAGTCTCCCCCTTCACCTGAA CTTACCTCAGAAGAAAACAAAATCCCAGATGCTGACAAAGCAAATGAAAAGAAAGTTGATCAGCCTCCAG AAGCCAAAAAACCTAAAATAAAGGTGGTAAACGTTGAGCTGCCTGTTGAAGCCAACTTGGTGTGGCAGCT AGGGAGGGACCTTCTGAACATGTACATCGAGACGGAGGGCAAGATGATCATGCAGGACAAGCTGGAGAAG GAGAGGAACGATGCTAAGAACGCCGTGGAGGAGTGTGTGTATGAGTTCAGAGACAAGCTGTGTGGACCGT ATGAGAAATTTATATGTGAGCAGGAACACGAGAAGTTTCTGAGGCTTCTCACAGAGACAGAAGACTGGCT GTATGAGGAAGGAGAGGACCAAGCCAAGCAGGCCTATATTGACAAGTTGGAAGAATTGATGAAAATGGGC ACTCCTGTAAAAGTCAGATTTCAAGAAGCCGAGGAGCGACCGAGAGTGTTGGAGGAGCTAGGCCAGCGCC TACAGCACTATGCCAAGATCGCAGCAGACTTCAGAGGCAAGGATGAGAAATACAACCACATAGATGAATC TGAAATGAAGAAGGTTGAGAAGTCTGTTAACGAGGTGATGGAGTGGATGAATAATGTCATGAATGCTCAG GCTAAACGGAGTCTCCATCAGGACCCTGTTGTGCGCACTCATGAGATCAGCGCTAAGGTCAAGGAACTGA ACAATGTTTGTGAACCTGTTGTAACTCAACCCAAACCAAAAATTGAGTCACCTAAACTGGAGAGAACTCC AAATGGCCCAAATATGGACAAGAAAGAAGATCTAGAAGGCAAGAGTAATCTCGGTGCTGACGCTCCACAT CAGAACGGTGAATGCCACCCTAATGAGAAGGGCTCTGTCAGCATGGACCTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213187 representing NM_001011901
Red=Cloning site Green=Tags(s) MSVVGLDVGSQSCYIAVARAGGIETIANEFSDRCTPSVISFGPKNRTIGVAAKNQQITHANNTVSSFKRF HGRAFNDPFIQKEKENLSYDLVPMKNGGVGIKVMYMDEDHLFSVEQITAMLLTKLKETAENNLKKPVTDC VISVPSFFTDAERRSVLDAAQIVGLNCLRLMNDMTAVALNYGIYKQDLPNADEKPRVVVFVDMGHSSFQV SACAFNKGKLKVLGTAFDPFLGGKNFDEKLVEHFCAEFKTKYKLDAKSKIRALLRLHQECEKLKKLMSSN STDLPLNIECFMNDKDVSAKMNRSQFEELCAELLQKIEVPLHLLMEQTHLKTEEVSAIEIVGGATRIPAV KERIARFFGKDVSTTLNADEAVARGCALQCAILSPAFKVREFSVTDAVPFPISLVWNHDSEETEGVHEVF SRNHAAPFSKVLTFLRRGPFELEAFYSDPQAVPYPEAKIGRFVVQNVSAQKDGEKSKVKVKVRVNTHGIF TISTASMVEKVPTEEEDGSSVEADMECPNQKPAESSDVDKNIQQDNSEAGTQPQVQTDGQQTSQSPPSPE LTSEENKIPDADKANEKKVDQPPEAKKPKIKVVNVELPVEANLVWQLGRDLLNMYIETEGKMIMQDKLEK ERNDAKNAVEECVYEFRDKLCGPYEKFICEQEHEKFLRLLTETEDWLYEEGEDQAKQAYIDKLEELMKMG TPVKVRFQEAEERPRVLEELGQRLQHYAKIAADFRGKDEKYNHIDESEMKKVEKSVNEVMEWMNNVMNAQ AKRSLHQDPVVRTHEISAKVKELNNVCEPVVTQPKPKIESPKLERTPNGPNMDKKEDLEGKSNLGADAPH QNGECHPNEKGSVSMDLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001011901 |
| ORF Size | 2574 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001011901.1, NP_001011901.1 |
| RefSeq Size | 3453 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 288444 |
| UniProt ID | Q66HA8 |
| MW | 96.4 kDa |
| Gene Summary | Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering substrate release. Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213187 | Hsph1 (untagged ORF) - Rat heat shock 105kDa/110kDa protein 1 (Hsph1), (10 ug) |
CNY 10,930.00 |
|
| RR213187L3 | Lenti ORF clone of Hsph1 (Myc-DDK-tagged ORF) - Rat heat shock 105kDa/110kDa protein 1 (Hsph1), (10 ug) |
CNY 12,830.00 |
|
| RR213187L4 | Lenti ORF clone of Hsph1 (mGFP-tagged ORF) - Rat heat shock 105kDa/110kDa protein 1 (Hsph1), (10 ug) |
CNY 13,970.00 |
