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Optn (NM_145081) Rat Tagged ORF Clone
CAT＃: RR213190
- TrueORF®
Optn (Myc-DDK-tagged ORF) - Rat optineurin (Optn), (10 ug)
"NM_145081" in other vectors (3)
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CNY 4,360.00
CNY 7,510.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fip2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213190 representing NM_145081
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCATCAACCTCTGAGCTGCCTGACTGAGAAGGGGGACAGCTCCTGTGAGACCCCAGGAAATGGAC CCTCCAATATGGTTCACCCCAACCTGGACACATTCACTCCTGAGGAGCTGCTGCAGCAAATGAAAGAACT CCTGGTCGAGAACCACCAGCTGAAAGAAGCCATGAAGCTAAATAATCAAGCTATGAAAGGGCGATTTGAG GAGCTTTCTGCCTGGACAGAGAGGCAGAAGGAAGAACGCCAGTTGTTTGAGATACAAAGCAAAGAGGCTA AGGAGCGCCTCAAGGCCCTGAGTCATGAAAATGAGAGGCTGAAGGAAGAGCTTGGAAAACTAAAAGAGAA ATCAGAAAGGCCATTTGAAGACATCACAGGTAGATGCGGGTTTCCCAGAACCGACTTGGAGCAGGAAGTG GGAGCAACTGAAGAGGCAGGTGGAGCAGGAAGTGGAGCATCTGAAGATCCAGGTGAGGCGCCTTCAGGCT TGAGAAAGCGGACCTTGCTGGGGCATTCGTCTCAGACTGCAGCTCAAGCTCAACTCCGGCGGCTCCTCAG AAGACTCCTTCGTGGAGACAGGATGACTGAAGGAGAGGCGGAAGGGGCAATGAAGGAGATGAGGAACAGC GCTGGACCCACAAGGACAGACTCCATCATCATGGGCAAATGTACAGAGGACGCCAGGACTTGTGTGGAGT TTGAGGAACTGACTGTGAGCCAACTCCTGCTTTGCCTCAGGGAAGGAAACCAAAAGGTGGAGAGACTCGA GATCGCGCTCAGAGAAGCCAAAGAAAGAATTTCAGATTTTGAAAAGAAAGCAAACGGCCATTCTGCGATT GAAACCCAGACAGAGGGGAGCACACAAAAAGAAGAGGAGGACAAAGACCCAGAGAGTGTGGGAATCGAAG TGGAAACTCTGAACGTTCAAGTGGCCTCTCTGTTTAAGGGGCTTCAAGAGGCGCACACAAAGCTCAGTGA GGCCGAGCTGATGAAGAAGAGACTTCAAGAAAAGTGTCAGGCTCTGGAAAGGAAGAACTCTGCAACCCCA TCGGAGCTGAATGAAAAGCAAGAGCTCGTTTACAGTAACTGGAAGTTAGAGCTGCAGGTGGAGAGCATGC GCTCGGAAATCAAGATGGAGCAGGCCAAGACAGAGGAGGAGAAATCCAGGTTAGCCACTCTTCAGGCAAC ACACGACAAGCTCCTTCAGGAACACAATAAAGCTCTGAGAACAATTGAAGAACTAACCAAACAACAGGCA GAAAAAGTGGACAAGGTGCAGCTGCAGGAGCTCAGCGAGAAGCTGGAGCTGGCGGAGCAGGCTCTGGCGT CCAAGCAGCTCCAGATGGATGAGATGAAGCAGACCATCGCCAAGCAGGAGGAGGACCTGGAGACCATGGC CGTCCTCAGGGCTCAGATGGAGGTGTACTGTTCAGATTTCCATGCTGAGAGGGCAGCAAGGGAGAAGATC CATGAGGAAAAGGAGCAGCTGGCCTTGCAGCTCGCCATTTTGCTGAAAGAGAACAATGACTTTGAAGATG GAGGCAGTAGGCAGTCCTTGATGGAAATGCAGTGCCGGCACGGGGCGAGAACCAGCGACTCTGACCAGCA GGCTTACCTGTTTCAAAGAGGAGCCAAGGACATGAGCTGGCAGCATGGGCAGCAGCCCCGGAGTATTCCC ATTCACTCGTGCCCCAAGTGTGGGGAGGTCCTGCCGGACATTGACACGCTTCAGATACATGTGATGGACT GTATCATCGTATCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213190 representing NM_145081
Red=Cloning site Green=Tags(s) MSHQPLSCLTEKGDSSCETPGNGPSNMVHPNLDTFTPEELLQQMKELLVENHQLKEAMKLNNQAMKGRFE ELSAWTERQKEERQLFEIQSKEAKERLKALSHENERLKEELGKLKEKSERPFEDITGRCGFPRTDLEQEV GATEEAGGAGSGASEDPGEAPSGLRKRTLLGHSSQTAAQAQLRRLLRRLLRGDRMTEGEAEGAMKEMRNS AGPTRTDSIIMGKCTEDARTCVEFEELTVSQLLLCLREGNQKVERLEIALREAKERISDFEKKANGHSAI ETQTEGSTQKEEEDKDPESVGIEVETLNVQVASLFKGLQEAHTKLSEAELMKKRLQEKCQALERKNSATP SELNEKQELVYSNWKLELQVESMRSEIKMEQAKTEEEKSRLATLQATHDKLLQEHNKALRTIEELTKQQA EKVDKVQLQELSEKLELAEQALASKQLQMDEMKQTIAKQEEDLETMAVLRAQMEVYCSDFHAERAAREKI HEEKEQLALQLAILLKENNDFEDGGSRQSLMEMQCRHGARTSDSDQQAYLFQRGAKDMSWQHGQQPRSIP IHSCPKCGEVLPDIDTLQIHVMDCIIVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145081 |
| ORF Size | 1758 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145081.3, NP_659549.2 |
| RefSeq Size | 3577 bp |
| RefSeq ORF | 1761 bp |
| Locus ID | 246294 |
| UniProt ID | Q8R5M4 |
| MW | 66.5 kDa |
| Gene Summary | an injury induced protein; human homolog may be linked to glaucoma [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213190 | Optn (untagged ORF) - Rat optineurin (Optn), (10 ug) |
CNY 7,510.00 |
|
| RR213190L3 | Lenti ORF clone of Optn (Myc-DDK-tagged ORF) - Rat optineurin (Optn), (10 ug) |
CNY 9,410.00 |
|
| RR213190L4 | Lenti ORF clone of Optn (mGFP-tagged ORF) - Rat optineurin (Optn), (10 ug) |
CNY 10,260.00 |
