Mapk8ip3 (NM_001100673) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	JIP3; JSAP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213400 representing NM_001100673 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAGATCCAGATGGACGAGGGAGGCGGTGTGGTGGTGTACCAAGATGACTACTGCTCCGGCTCGG TGATGTCGGAGCGTGTGTCGGGCCTGGCGGGCTCCATCTACCGCGAGTTCGAGCGCCTCATTCACTGCTA TGATGAGGAGGTGGTCAAGGAGCTCATGCCGCTGGTGGTGAACGTGCTGGAGAACCTTGACTCGGTGCTG AGCGAGAACCAGGAGCACGAGGTGGAGCTGGAGCTCCTACGCGAGGACAACGAGCAGCTGCTCACGCAAT ACGAGCGCGAGAAGGCGCTGCGCAAACAGGCCGAGGAGAAATTCATCGAATTTGAAGATGCCTTGGAACA AGAGAAGAAAGAACTCCAGATCCAGGTAGAACATTATGAGTTTCAGACACGCCAGCTGGAGCTAAAGGCC AAAAACTATGCAGATCAGATTTCCCGACTGGAGGAGCGGGAGTCGGAGATGAAGAAGGAATACAACGCGC TGCACCAGCGGCACACGGAGATGATCCAGACCTATGTGGAACACATCGAAAGATCGAAGATGCAGCAGGT TGGGGGAGGCGGCCAAACAGAAAGCAGCCTGCCCGGGCGGAGCAGGAAGGAGCGTCCCACCTCTCTGAAT GTCTTCCCCCTGGCTGATGGAATGGTACGTGCACAGATGGGGGGCAAGCTCGTGCCTGCGGGGGACCACT GGCACCTGAGTGACCTCGGCCAACTACAGTCCAGCTCCAGCTACCAGTGTCCAAACGATGAGATGTCTGA GTCAGGCCAGTCCTCAGCAGCTGCAACACCCAGTACCACAGGTACCAAATCCAACACACCCACATCCTCC GTGCCCTCAGCAGCTGTCACACCACTCAACGAGAGCCTACAGCCCCTGGGGGACTATGGCAGTGTCACAA AGAACAACAAACGGGCCCGAGAGAAGCGCAACAGCCGTAACATGGAGGTCCAGGTCACCCAAGAGATGCG GAACGTCAGTATTGGTATGGGTAGCAGTGACGAGTGGTCCGATGTTCAGGACATTATCGACTCCACCCCA GAGCTGGATGTGTGTCCGGAAACCCGTCTGGACCGCACAGGAAGCAGCCCAACCCAGGGGATCGTAAACA AAGCTTTTGGAATCAACACCGATTCCCTGTACCATGAACTATCTACAGCGGGGTCTGAGGTCATCGGGGA TGTGGACGAGGGAGCTGATCTTCTAGGGGAGTTCTCAGGAATGGGCAAAGAAGTGGGGAACCTGCTGCTG GAGAACTCACAGCTTCTAGAGACAAAAAATGCTTTGAATGTGGTGAAGAATGACCTCGTTGCCAAGGTTG ACCAACTGTCAGGAGAGCAGGAGGTACTGAAGGGTGAATTGGAAGCAGCCAAGCAAGCCAAAGTCAAGCT GGAGAACCGAATCAAAGAGCTTGAAGAAGAACTAAAAAGAGTCAAGTCAGAGGCAGTAACCGCCCGCCGT GAGCCCAGAGAAGAGGTGGAGGATGACAAAATCCCCATGGCCCAACGCCGACGCTTCACAAGAGTGGAGA TGGCCCGAGTGCTCATGGAACGTAACCAGTACAAGGAACGCCTCATGGAGCTGCAGGAGGCTGTGCGGTG GACTGAAATGATCAGAGCATCCAGGGAACACCCATCTGTCCAGGAAAAGAAGAAATCCACTATCTGGCAG TTCTTTAGCCGCCTCTTCAGCTCCTCATCCAGCCCCCCTCCGGCCAAACGGTCCTACCCGTCTGTGAACA TTCACTACAAGTCACCCACTACAGCTGGCTTTAGCCAGCGTCGCAACCATGCTTTGTGCCAGATCTCAGC CGGCAGCCGACCCCTGGAGTTCTTCCCTGATGATGACTGCACCTCTTCTGCCCGGCGTGAGCAGAAGCGG GAGCAGTACCGCCAGGTCCGTGAACACGTGCGCAATGATGACGGGAGGCTGCAGGCCTGTGGGTGGAGCC TGCCTGCCAAGTACAAGCAGCTGAGCCCCAATGGAGGCCAGGAAGATACCCGGATGAAAAATGTGCCTGT CCCTGTGTACTGCCGCCCTCTGGTGGAGAAGGACCCCTCAACAAAGCTGTGGTGTGCTGCTGGTGTCAAC CTGAGTGGGTGGAAACCAAACGAGGAGGACTCTAGCAATGGACCCAAGCCTGCACCAGGTCGAGACCCTC TGACCTGCGACCGGGAAGGAGAAGGCGAACCCAAGAGCACCCACCCATCACCTGAGAAGAAGAAGGCAAA GGAGGTCCCTGAGGCGGATGCTACCTCCAGTCGGGTATGGATCCTCACCAGCACCCTGACTACCAGCAAG GTGGTGATCATTGATGCCAACCAACCAGGCACAGTTGTGGATCAGTTCACAGTCTGCAATGCCCACGTCC TGTGTATCTCCAGCATTCCTGCGGCCAGCGACAGTGATTATCCCCCTGGAGACATGTTCCTAGACAGTGA TGTGAACCCCGAAGACTCAGGTGCTGACGGTGTGCTGGCTGGCATCACCCTGGTGGGATGTGCTACCCGC TGCAATGTACCACGTAGCAACTGTTCCTCCCGAGGAGACACCCCAGTACTGGACAAGGGGCAGGGGGATG TGGCAGCCACTGCCAATGGGAAGGTCAACCCATCCCAATCCACAGAAGAAGCCACAGAAGCCACGGAGGT GCCAGATCCTGGTCCCAGTGAGTCAGAAGCAACAACAGTCCGGCCTGGGCCTCTCACAGAGCATGTCTTT ACTGACCCAGCACCCACCCAATCCTCCAGCACCCAGCCTGCCAGTGAGAATGGGTCAGAGTCGGATGGCA GCATTGTACAGCCTCAGATGGAGCCCAGTGGGGAGTCCTCAGCAACCACCAGTAGTGCTGCACCCACCAT GTGGCTAGGAGCCCAGAATGGCTGGCTCTATGTGCACTCAGCGGTAGCCAACTGGAAGAAGTGTCTGCAC TCCATCAAACTAAAAGACTCTGTGCTGAGCCTGGTGCATGTCAAAGGCCGAGTGCTGGTAGCTCTTGCAG ATGGAACCCTGGCTATCTTCCATCGTGGAGAGGATGGCCAGTGGGACCTGAGCAACTACCACCTAATGGA CCTGGGCCACCCACACCACTCCATCCGCTGCATGGCTGTTGTGGATGACCGAGTTTGGTGTGGCTACAAG AACAAGGTGCATGTCATCCAGCCCAAGACAATGCAAATTGAGAAATCATTTGATGCCCACCCACGGCGGG AAAGCCAGGTACGACAGCTAGCCTGGATCGGTGATGGAGTATGGGTCTCTATTCGCTTGGATTCTACACT TCGGCTGTACCACGCTCACACGCACCAGCACCTGCAGGATGTGGACATTGAGCCCTATGTTAGCAAGATG CTGGGTACCGGCAAGCTGGGCTTCTCCTTCGTGCGCATCACAGCCCTACTCATTGCAGGCAACCGTCTGT GGGTGGGCACTGGCAATGGGGTTGTCATCTCCATCCCCTTGACTGAGACTGTGGTCCTGCATCGAGGCCA GCTCTTAGGTCTCCGAGCCAACAAGACATCCCCAACGTCTGGGGAGGGGACCCGCCCAGGGGGCATCATC CATGTGTATGGGGATGACAGCAGTGACAAGACTGCCAGTAGTTTCATCCCCAACTGCTCCATGGCCCAGG CTCAGCTCTGCTTCCACGGGCACCGTGATGCTGTCAAATTCTTTGTCTCTGTACCAGGAAATGTGCTGGC CACGCTCAATGGCAGTGTGCTAGACAGCCCATCAGAGGGCCCTGGGCCTGCTGCACCTGCTGCAGATGCT GAGGGCCAGAAGTTGAAGAATGCACTGGTGCTGAGTGGTGGTGAAGGTTACATTGACTTCCGCATCGGGG ATGGAGAGGATGATGAAACTGAGGAAGGTACCGGGGATGTGAACCAGACGAAGCCCTCGTTGTCCAAGGC TGAGCGCAGCCACATCATCGTGTGGCAGGTGTCCTACACCCCTGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213400 representing NM_001100673 Red=Cloning site Green=Tags(s) MMEIQMDEGGGVVVYQDDYCSGSVMSERVSGLAGSIYREFERLIHCYDEEVVKELMPLVVNVLENLDSVL SENQEHEVELELLREDNEQLLTQYEREKALRKQAEEKFIEFEDALEQEKKELQIQVEHYEFQTRQLELKA KNYADQISRLEERESEMKKEYNALHQRHTEMIQTYVEHIERSKMQQVGGGGQTESSLPGRSRKERPTSLN VFPLADGMVRAQMGGKLVPAGDHWHLSDLGQLQSSSSYQCPNDEMSESGQSSAAATPSTTGTKSNTPTSS VPSAAVTPLNESLQPLGDYGSVTKNNKRAREKRNSRNMEVQVTQEMRNVSIGMGSSDEWSDVQDIIDSTP ELDVCPETRLDRTGSSPTQGIVNKAFGINTDSLYHELSTAGSEVIGDVDEGADLLGEFSGMGKEVGNLLL ENSQLLETKNALNVVKNDLVAKVDQLSGEQEVLKGELEAAKQAKVKLENRIKELEEELKRVKSEAVTARR EPREEVEDDKIPMAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASREHPSVQEKKKSTIWQ FFSRLFSSSSSPPPAKRSYPSVNIHYKSPTTAGFSQRRNHALCQISAGSRPLEFFPDDDCTSSARREQKR EQYRQVREHVRNDDGRLQACGWSLPAKYKQLSPNGGQEDTRMKNVPVPVYCRPLVEKDPSTKLWCAAGVN LSGWKPNEEDSSNGPKPAPGRDPLTCDREGEGEPKSTHPSPEKKKAKEVPEADATSSRVWILTSTLTTSK VVIIDANQPGTVVDQFTVCNAHVLCISSIPAASDSDYPPGDMFLDSDVNPEDSGADGVLAGITLVGCATR CNVPRSNCSSRGDTPVLDKGQGDVAATANGKVNPSQSTEEATEATEVPDPGPSESEATTVRPGPLTEHVF TDPAPTQSSSTQPASENGSESDGSIVQPQMEPSGESSATTSSAAPTMWLGAQNGWLYVHSAVANWKKCLH SIKLKDSVLSLVHVKGRVLVALADGTLAIFHRGEDGQWDLSNYHLMDLGHPHHSIRCMAVVDDRVWCGYK NKVHVIQPKTMQIEKSFDAHPRRESQVRQLAWIGDGVWVSIRLDSTLRLYHAHTHQHLQDVDIEPYVSKM LGTGKLGFSFVRITALLIAGNRLWVGTGNGVVISIPLTETVVLHRGQLLGLRANKTSPTSGEGTRPGGII HVYGDDSSDKTASSFIPNCSMAQAQLCFHGHRDAVKFFVSVPGNVLATLNGSVLDSPSEGPGPAAPAADA EGQKLKNALVLSGGEGYIDFRIGDGEDDETEEGTGDVNQTKPSLSKAERSHIIVWQVSYTPE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100673
ORF Size	3966 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100673.1, NP_001094143.1
RefSeq Size	3969 bp
RefSeq ORF	3969 bp
Locus ID	302983
UniProt ID	E9PSK7
MW	145.6 kDa
Gene Summary	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity). Promotes neuronal axon elongation in a kinesin- and JNK-dependent manner. Activates cofilin at axon tips via local activation of JNK, thereby regulating filopodial dynamics and enhancing axon elongation. Its binding to kinesin heavy chains (KHC), promotes kinesin-1 motility along microtubules and is essential for axon elongation and regeneration. Regulates cortical neuronal migration by mediating NTRK2/TRKB anterograde axonal transport during brain development. Acts as an adapter that bridges the interaction between NTRK2/TRKB and KLC1 and drives NTRK2/TRKB axonal but not dendritic anterograde transport, which is essential for subsequent BDNF-triggered signaling and filopodia formation.[UniProtKB/Swiss-Prot Function]

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