Pnlip (NM_013161) Rat Tagged ORF Clone
CAT#: RR213572
- TrueORF®
Pnlip (Myc-DDK-tagged ORF) - Rat pancreatic lipase (Pnlip), (10 ug)
"NM_013161" in other vectors (3)
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CNY 6,080.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PANLI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR213572 representing NM_013161
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGATGCTGTGGACATTTGCAGTGCTGCTGGGAGCAGTAGCTGGAAAAGAAGTTTGCTTTGACAAAC TCGGCTGCTTCAGTGATGATGCCCCGTGGTCAGGAACCATAGACAGACCCCTGAAAGCACTGCCCTGGAG CCCAGCACAGATCAACACCCGCTTTCTCCTCTACACCAACGAGAACCAAGACAACTACCAGAAAATTACT TCAGATGCATCGAGTATCAGAAACTCCAATTTCAAAACGAACAGAAAAACCCGCATCATTATCCACGGCT TCATTGACAAGGGAGAGGAGAACTGGCTATCTGACATGTGCAAGAACATGTTCAAAGTGGAGAGTGTGAA CTGCATCTGTGTGGACTGGAAAGGTGGCTCCCGGGCTACATACACCCAGGCTACCCAGAACGTCCGGGTC GTGGGTGCAGAAGTGGCATTATTGGTTAACGTTCTGAAGTCTGACCTCGGTCATCCACCTGACAACGTCC ACCTGATTGGCCACAGCCTGGGTTCCCACGTTGCTGGGGAGGCAGGAAAGAGGACATTCGGAGCTATTGG GAGGATCACAGGGTTGGACGCGGCTGAACCTTACTTCCAGGGCACACCTGAAGAAGTCCGTCTGGACCCC ACCGATGCCCAGTTTGTGGATGCAATTCACACAGATGCTGCACCCATCATCCCCAACTTAGGATTTGGAA TGAGCCAGACTGTGGGTCACCTAGATTTCTTTCCTAATGGAGGGATGGAAATGCCTGGATGTCAGAAGAA TATCCTTTCCCAAATCGTTGACATCGACGGGATCTGGGAAGGAACTCGCGACTTTGCTGCCTGTAACCAC TTGAGAAGCTACAAATATTACACCGACAGTATCGTCAACCCGACTGGCTTCTCTGGCTTCTCCTGTAGCT CTTACAATGTTTTTTCTGCAAATAAGTGCTTCCCATGTGGGAGTGAAGGATGCCCACAGATGGGTCACTA TGCTGACAAATACCCTGGGAAGACAAAAGAACTCTACCAGAAATTTTACCTGAACACTGGTGACAAGAGC AACTTCGCACGTTGGCGGTACCAGGTGACTGTCACCCTCTCTGGACAGAAGGTCACTGGGCACATTCTAG TTTCTTTGTTTGGAAATGGAGGAAACTCTAAACAGTATGAAGTTTTCAAGGGCTCTCTGCATCCAGGTGA CACTCATGTCAAGGAATTTGACTCTGATATGGATGTTGGAGATTTGCAGAAAGTTAAATTTATTTGGTAC AACAATGTGATCAACCCAACTCTACCCAAAGTGGGAGCATCAAGGATTTCAGTGGAACGAAATGACGGCA GAGTGTTCAACTTCTGTAGTCAAGATACAGTGAGGGAGGATGTCCTGCTTACACTGTCTGCATGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213572 representing NM_013161
Red=Cloning site Green=Tags(s) MLMLWTFAVLLGAVAGKEVCFDKLGCFSDDAPWSGTIDRPLKALPWSPAQINTRFLLYTNENQDNYQKIT SDASSIRNSNFKTNRKTRIIIHGFIDKGEENWLSDMCKNMFKVESVNCICVDWKGGSRATYTQATQNVRV VGAEVALLVNVLKSDLGHPPDNVHLIGHSLGSHVAGEAGKRTFGAIGRITGLDAAEPYFQGTPEEVRLDP TDAQFVDAIHTDAAPIIPNLGFGMSQTVGHLDFFPNGGMEMPGCQKNILSQIVDIDGIWEGTRDFAACNH LRSYKYYTDSIVNPTGFSGFSCSSYNVFSANKCFPCGSEGCPQMGHYADKYPGKTKELYQKFYLNTGDKS NFARWRYQVTVTLSGQKVTGHILVSLFGNGGNSKQYEVFKGSLHPGDTHVKEFDSDMDVGDLQKVKFIWY NNVINPTLPKVGASRISVERNDGRVFNFCSQDTVREDVLLTLSAC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013161 |
ORF Size | 1395 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013161.2, NP_037293.1 |
RefSeq Size | 1491 bp |
RefSeq ORF | 1398 bp |
Locus ID | 25702 |
UniProt ID | P27657 |
MW | 51.4 kDa |
Gene Summary | Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN213572 | Pnlip (untagged ORF) - Rat pancreatic lipase (Pnlip), (10 ug) |
CNY 6,080.00 |
|
RR213572L3 | Lenti ORF clone of Pnlip (Myc-DDK-tagged ORF) - Rat pancreatic lipase (Pnlip), (10 ug) |
CNY 7,890.00 |
|
RR213572L4 | Lenti ORF clone of Pnlip (mGFP-tagged ORF) - Rat pancreatic lipase (Pnlip), (10 ug) |
CNY 8,650.00 |