Smc3 (NM_031583) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bamacan; Cspg6; SMC-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213612 representing NM_031583 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACATCAAGCAGGTGATTATCCAGGGCTTCCGAAGTTACCGAGATCAAACAATTGTAGATCCCTTCA GTTCAAAACATAATGTTATTGTGGGCAGAAATGGATCTGGAAAAAGCAACTTTTTTTATGCAATCCAGTT TGTTCTCAGTGATGAATTTAGTCATCTTCGTCCAGAGCAGCGATTGGCTTTGTTGCACGAGGGTACAGGT CCTCGTGTTATTTCTGCTTTTGTGGAAATCATTTTTGACAATTCAGACAACCGGTTACCAATTGATAAAG AGGAAGTTTCGCTTCGAAGAGTTATTGGTGCCAAAAAAGATCAGTATTTCCTAGATAAGAAGATGGTCAC GAAAAATGATGTGATGAATCTCCTTGAAAGTGCTGGGTTTTCGAGAAGTAATCCTTATTACATTGTTAAA CAAGGAAAGATCAACCAAATGGCAACAGCACCGGATTCTCAGAGATTAAAGCTGTTGAGAGAAGTAGCTG GCACTCGAGTGTATGACGAGCGCAAAGAGGAAAGCATCTCCCTGATGAAAGAAACAGAGGGCAAACGGGA AAAGATCAATGAGTTGTTAAAATACATTGAAGAGCGATTACATACTCTAGAGGAGGAGAAGGAGGAACTG GCCCAGTATCAGAAGTGGGATAAAATGAGGCGTGCCCTGGAGTACACCATCTACAACCAGGAGCTCAACG AGACTCGCGCCAAGCTTGATGAGCTTTCTGCTAAGCGAGAAACAAGCGGAGAGAAATCCAGACAATTAAG AGATGCCCAGCAGGATGCAAGAGATAAAATGGAGGATATTGAGCGCCAAGTTAGAGAACTGAAAACAAAA ATTTCAGCCATGAAAGAAGAAAAAGAACAGCTCAGTGCGGAGAGACAAGAGCAGATTAAGCAAAGGACCA AGCTGGAGCTTAAAGCCAAGGACTTACAGGATGAGCTGGCAGGGAACAGTGAGCAGCGGAAACGTTTATT AAAAGAAAGACAGAAGCTGCTTGAAAAAATAGAAGAAAAGCAGAAAGAACTGGCAGAAACAGAACCGAAA TTCAACAGCGTAAAGGAAAAAGAAGAGCGAGGAATTGCTAGATTGGCTCAAGCTACACAGGAAAGAACTG ATCTTTATGCAAAGCAGGGTCGAGGAAGCCAGTTTACATCAAAAGAAGAAAGAGATAAGTGGATTAAGAA GGAACTGAAGTCTTTAGATCAGGCTATTAATGATAAGAAAAGACAGATTGCTGCTATACACAAGGATTTG GAGGATACCGAGGCCAATAAAGAGAAAAATCTGGAGCAATATAATAAACTGGATCAGGATCTCAATGAAG TCAAAGCTCGAGTTGAAGAACTGGACAGAAAATATTATGAAGTGAAAAATAAGAAAGATGAACTACAAAG TGAAAGAAATTACTTGTGGAGAGAGGAGAATGCAGAACAGCAAGCACTTGCCGCTAAGAGAGAAGACCTT GAGAAGAAGCAGCAGCTTCTTAGAGCGGCAACAGGAAAGGCCATTTTAAATGGAATCGACAGCATTAACA AAGTGTTAGACCATTTCCGGCGGAAGGGTATAAACCAGCATGTGCAAAATGGCTACCATGGCATCGTGAT GAATAACTTTGAGTGCGAACCAGCTTTCTATACTTGTGTGGAAGTCACTGCCGGTAACAGGTTATTCTAT CACATTGTCGATTCAGATGAAGTCAGCACGAAGATCTTAATGGAGTTCAATAAAATGAATCTTCCTGGAG AGGTGACTTTCCTGCCTCTTAACAAGTTAGATGTGAGGGACACTGCCTATCCTGAAACTAACGATGCTAT TCCTATGATCAGTAAGCTGAGGTACAATCCCAGATTCGACAAAGCTTTCAAACATGTGTTTGGAAAGACA CTCATCTGTCGGAGCATGGAGGTTTCCACTCAGCTAGCTCGTGCCTTCACTATGGACTGCATTACTCTGG AAGGTGATCAAGTCAGCCATCGAGGTGCTCTGACTGGAGGCTATTACGACACAAGAAAGTCTCGCCTTGA GTTACAGAAGGATGTTAGGAAAGCAGAGGAGGAGCTGGGTGAGCTCGAAGCTAAGCTCAATGAAAACCTG CGCAGAAACATTGAAAGGATTAATAATGAAATTGATCAATTGATGAACCAAATGCAGCAGATAGAGACCC AACAGAGAAAATTTAAAGCATCCAGAGATAGCATCTTATCAGAGATGAAGATGCTAAAAGAGAAGAGACA GCAGTCAGAAAAGACCTTCATGCCAAAGCAACGCAGTTTGCAGAGTCTGGAGGCCAGTCTGCACGCTATG GAGTCGACCAGAGAGTCGCTGAAGGCAGAGCTAGGGACAGATTTGCTCTCTCAGCTCAGCCTGGAAGACC AGAAAAGAGTCGACGCACTGAATGACGAGATCCGGCAGCTTCAGCAGGAAAACAGACAGCTGCTGAATGA AAGAATTAAACTAGAAGGCATTATTACTCGAGTAGAGACTTACCTCAATGAGAATCTGAGGAAACGTCTG GACCAAGTAGAGCAGGAACTTAATGAACTGAGAGAGACAGAAGGTGGCACTGTTCTTACTGCCACCACGT CAGAACTTGAAGCTATTAATAAAAGAGTGAAAGATACTATGGCAAGATCAGAAGATTTGGATAATTCCAT TGACAAAACAGAAGCTGGAATTAAAGAGCTCCAGAAAAGCATGGAGCGCTGGAAGAACATGGAGAAGGAG CACATGGATGCCATAAATCACGACACTAAAGAGCTGGAGAAGATGACCAACCGACAGGGCATGCTGTTGA AGAAGAAAGAGGAGTGCATGAAGAAGATCCGAGAGCTGGGGTCCCTTCCCCAGGAAGCATTTGAAAAGTA CCAGACACTGAGCCTGAAGCAGTTGTTTCGGAAACTTGAGCAGTGCAACACAGAGTTGAAGAAGTACAGC CATGTCAACAAGAAGGCTCTGGACCAGTTTGTGAATTTCTCTGAGCAGAAGGAGAAACTGATAAAGCGAC AGGAAGAATTGGACAGGGGCTACAAGTCAATCATGGAGTTGATGAATGTACTCGAACTTAGAAAATATGA AGCTATCCAGTTAACCTTCAAACAGGTATCTAAGAACTTTAGTGAAGTTTTCCAGAAGTTGGTACCTGGG GGAAAAGCGACCTTGGTGATGAAGAAAGGAGACGTGGAGGGCAGCCAGTCTCAGGACGAAGGAGAGGGGA GCGGCGAGAGCGAGAGGGGCTCTGGGTCACAAAGCAGCGTTCCATCAGTTGACCAGTTCACAGGAGTCGG GATCAGGGTATCATTTACAGGAAAGCAAGGCGAGATGAGAGAAATGCAGCAGCTTTCAGGAGGGCAGAAG TCTCTGGTAGCCCTTGCTCTCATTTTTGCCATTCAGAAGTGTGACCCTGCTCCTTTCTACCTGTTTGATG AGATCGACCAAGCTTTGGATGCTCAGCACAGAAAAGCTGTGTCAGATATGATTATGGAACTAGCTGTACA CGCCCAGTTTATCACTACCACTTTTAGGCCTGAACTGCTTGAGTCTGCTGATAAATTCTACGGTGTAAAG TTCAGAAATAAGGTTAGTCACATCGACGTGATCACAGCAGAGATGGCCAAAGACTTTGTAGAAGACGATA CCACGCATGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213612 representing NM_031583 Red=Cloning site Green=Tags(s) MYIKQVIIQGFRSYRDQTIVDPFSSKHNVIVGRNGSGKSNFFYAIQFVLSDEFSHLRPEQRLALLHEGTG PRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVK QGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEEL AQYQKWDKMRRALEYTIYNQELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTK ISAMKEEKEQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELAETEPK FNSVKEKEERGIARLAQATQERTDLYAKQGRGSQFTSKEERDKWIKKELKSLDQAINDKKRQIAAIHKDL EDTEANKEKNLEQYNKLDQDLNEVKARVEELDRKYYEVKNKKDELQSERNYLWREENAEQQALAAKREDL EKKQQLLRAATGKAILNGIDSINKVLDHFRRKGINQHVQNGYHGIVMNNFECEPAFYTCVEVTAGNRLFY HIVDSDEVSTKILMEFNKMNLPGEVTFLPLNKLDVRDTAYPETNDAIPMISKLRYNPRFDKAFKHVFGKT LICRSMEVSTQLARAFTMDCITLEGDQVSHRGALTGGYYDTRKSRLELQKDVRKAEEELGELEAKLNENL RRNIERINNEIDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKRQQSEKTFMPKQRSLQSLEASLHAM ESTRESLKAELGTDLLSQLSLEDQKRVDALNDEIRQLQQENRQLLNERIKLEGIITRVETYLNENLRKRL DQVEQELNELRETEGGTVLTATTSELEAINKRVKDTMARSEDLDNSIDKTEAGIKELQKSMERWKNMEKE HMDAINHDTKELEKMTNRQGMLLKKKEECMKKIRELGSLPQEAFEKYQTLSLKQLFRKLEQCNTELKKYS HVNKKALDQFVNFSEQKEKLIKRQEELDRGYKSIMELMNVLELRKYEAIQLTFKQVSKNFSEVFQKLVPG GKATLVMKKGDVEGSQSQDEGEGSGESERGSGSQSSVPSVDQFTGVGIRVSFTGKQGEMREMQQLSGGQK SLVALALIFAIQKCDPAPFYLFDEIDQALDAQHRKAVSDMIMELAVHAQFITTTFRPELLESADKFYGVK FRNKVSHIDVITAEMAKDFVEDDTTHG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031583
ORF Size	3651 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031583.2, NP_113771.2
RefSeq Size	4158 bp
RefSeq ORF	3654 bp
Locus ID	29486
MW	141.5 kDa
Gene Summary	core protein of a chondroitin sulfate proteoglycan that is a component of basement membranes [RGD, Feb 2006]

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