Lefty1 (NM_001109080) Rat Tagged ORF Clone
CAT#: RR213636
- TrueORF®
Lefty1 (Myc-DDK-tagged ORF) - Rat left right determination factor 1 (Lefty1), (10 ug)
"NM_001109080" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | RGD1561867 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR213636 representing NM_001109080
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGTTCCTATGGCTCTGTTGGGCACTCTGGGCACTATTCCAGGTTAGCCCTGGAGAAGCCCTGACTG GAGAGCAGGTCCTGGGCAGCCTTCTGCAACAGCTGCGGCTTGATCGACCGCCAGTCTTGGACAAGGCTGA TGTGGAGGGAATGGTCATCCCCTCGCATGTGAGGGCCCAGTATGTGGCCCTGCTCCAGCACAGCCATGAC AGCCGCTCCCGAGGCAAGAGGTTTAGCCAGAACTTTCGAGAGGTGGCAGGCAGGTTCCTGGTGTCAGAGA CCTCCAGTCACCTGCTGGTGTTCGGCATGGAGCAGCGGCTGCCGCCTAACAGTGAGCTGGTGCAGGCTGT GCTGCGGCTGTTCCAGGAGCCTGTGCCCAGAACAGCTCTCCGGAGGCAAAAGAGGCTGTCCCCACACAGC GCCCGGGCTCGGGTCACCATTGAATGGCTGCGCTTCCGCGAGGATGGCTCCAACCGTACCGCCCTTATCG ATTCTAGGCTCGTGTCCATCCACGAGAGCGGCTGGAAGGTCTTCGATGTGACCGAGGCCGTGAACTTCTG GCAGCAGCTGAGCCGGCCGAGGCAGCCGCTGCTGCTCCAGGTGTCGGTGCAGAGGGAGCATCTGGGACCG GGGACCTGGAGCGCACACAAGTTGGTCCGGTTCGCGGCGCAGGGGACGCCGGACGGCAAGGGGCTGGGCG AGCCACAGCTGGAGCTGCACACGCTGGACCTCAAGGACTATGGGGCTCAAGGCAATTGTGACCCCGAGAC ACCAGTGACTGAAGGCACCCGATGCTGTCGCCAAGAGATGTACCTGGACCTGCAGGGGATGAAGTGGGCT GAGAACTGGATCCTAGAACCCCCAGGATTCCTGACTTACGAATGTGTGGGCAGCTGCCTGCAGCCACCAG AGTCCCTGACCATCAGGTGGCCATTTCTGGGGCCTCGGCAGTGTGTGGCCTCAGAGATGACTTCCTTGCC CCTGATTGTCAGCATAAAGGAGGACGGCAGGACCAGGCCTCAAGTGGTCAGCCTGCCCAACATGAGGGTG CAGAGGTGTAGCTGTGCCTCGGATGGGGCGCTCATACCCAGGAGGCTGGAGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213636 representing NM_001109080
Red=Cloning site Green=Tags(s) MRFLWLCWALWALFQVSPGEALTGEQVLGSLLQQLRLDRPPVLDKADVEGMVIPSHVRAQYVALLQHSHD SRSRGKRFSQNFREVAGRFLVSETSSHLLVFGMEQRLPPNSELVQAVLRLFQEPVPRTALRRQKRLSPHS ARARVTIEWLRFREDGSNRTALIDSRLVSIHESGWKVFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGP GTWSAHKLVRFAAQGTPDGKGLGEPQLELHTLDLKDYGAQGNCDPETPVTEGTRCCRQEMYLDLQGMKWA ENWILEPPGFLTYECVGSCLQPPESLTIRWPFLGPRQCVASEMTSLPLIVSIKEDGRTRPQVVSLPNMRV QRCSCASDGALIPRRLEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001109080 |
ORF Size | 1104 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001109080.1, NP_001102550.1 |
RefSeq Size | 1572 bp |
RefSeq ORF | 1107 bp |
Locus ID | 498299 |
MW | 41.8 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN213636 | Lefty1 (untagged ORF) - Rat left right determination factor 1 (Lefty1), (10 ug) |
CNY 3,990.00 |
|
RR213636L3 | Lenti ORF clone of Lefty1 (Myc-DDK-tagged ORF) - Rat left right determination factor 1 (Lefty1), (10 ug) |
CNY 6,080.00 |
|
RR213636L4 | Lenti ORF clone of Lefty1 (mGFP-tagged ORF) - Rat left right determination factor 1 (Lefty1), (10 ug) |
CNY 6,650.00 |