Magi3 (NM_139084) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Slipr
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213790 representing NM_139084 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAAGACGTTGAAGAAGAAGAAGCATTGGCTCAGCAAGGTGCAGGAGTGTGCGGTGTCCTGGGCCG GGCCCCCGGGCGACTTGGGCGCTGAGATCCGCGGCGGCGCGGAGCGCGGCGAGTTCCCTTACCTGGGGCG GCTCCGTGACGAGCCTGGCGGCGGCGGAGGCACCTGCTGCGTGGTCTCGGGCAAGGCGCCCAGTCCTGGC GATGTGCTGTTGGAGGTGAACGGGACGCCCGTCAGTGGGCTCACCAACCGGGACACCCTGGCTGTCATCC GCCACTTCCGCGAGCCAATCCGTCTGAAGACCGTGAAGCCAGGCAAAGTCATAAATAAAGATTTGCGACA TTACCTGAGTCTGCAGTTTCAGAAAGGATCGATTGACCACAAACTACAGCAGGTCATCAGAGATAACCTC TACTTGATAACCATCCCATGCACTACAAGGGCTCCCAGGGATGGGGAAGTTCCAGGGGTGGACTATAATT TCATTTCTGTTGAACAATTCAAGGCACTGGAAGAAAGCGGAGCATTGTTAGAAAGTGGAACGTATGATGG AAATTTCTATGGCACGCCCAAGCCTCCAGCAGAGCCTAGTCCTTTCCAGCCCGATCCAGTTGATCAGGTC CTCTTTGACAATGAGTTTGACACAGAATCCCAAAGAAAACGAACTACATCTGTCAGCAAGATGGAAAGGA TGGACAGCTCTCTCCCTGAGGAAGAGGAAGATGAGGACAAAGAAGCTGTCAATGGCAGCGGAAGCATGGA AACTAGAGAGATGCATTCTGAGTCGTCTGACTGCTGGATGAAGACTGTTCCAAGTTATAACCAAACAAAT CGCTCCATGGACTTTAGAAATTATATGATGAGAGACGAGAATCTGGAGCCGCTGCCCAAAAACTGGGAAA TGGCCTACACTGACACAGGAACGATCTACTTCATTGATCACAATACGAAGACAACCACCTGGCTGGATCC TCGTCTCTGCAAGAAAGCCAAAGCCCCTGAAGACTGTGAGGATGGAGAGCTTCCATATGGCTGGGAGAAA ATAGAGGACCCTCAGTATGGAACATACTATGTTGATCACCTCAACCAGAAAACTCAGTTTGAAAATCCAG TGGAGGAAGCGAAAAGGAAAAAGCAGATAGGACAGGCTGAAACTCATTCCGCAAAAACAGATGTGGAAAG AGCACACTTTACCCGGGACCCATCCCAACTTAAAGGTGTCCTTGTTCGAGCATCACTGAAAAAAAGCACA ATGGGCTTTGGTTTCACCATTATAGGTGGAGATCGGCCTGATGAATTTCTACAAGTAAAAAATGTGCTCA AAGACGGTCCCGCTGCTCAGGATGGGAAAATGGCACCAGGTGATGTTATTGTAGACATCAATGGCAACTG TGTCCTTGGTCACACCCATGCAGATGTTGTCCAGATGTTTCAATTGGTACCTGTCAACCAGTATGTCAAC CTTACTTTATGCCGTGGTTATGCACTTCCTGACGACAGTGAAGACCCTGTTGTGGACATTGTTGCTGCTA CCCCTGTTATCAATGGACAATCATTAGCAAAGGGAGAGGCGTGCATGAGTACTCAGGATTTTAAACTGGG AGCAATGGTTTTGGATCAGAATGGAAAATCAGGAAAACTTTTGTCCAGTGATCGTCTCAATGGCCCATCA GATTCAAATGAGCAAAGAGCATCCCTGGCTTCATCAGGCAGCTCCCAGCCTGAACTAGTGACTATCCCGC TGGTTAAGGGTCCCAAAGGCTTCGGGTTTGCAATTGCCGATAGCCCAACTGGGCAGAAGGTGAAAATGAT ACTGGATAGCCAGTGGTGTCAAGGCCTTCAAAAAGGGGATATCATCAAAGAAATTTACCATCAGAATGTG CAGAATTTAACACATCTCCAAGTCGTAGAAGTACTGAAACAGTTCCCAGTAGGTGCAGATGTCCCGTTGC TTATCTTAAGAGGAGGTCCTTGTTCACCAACTAAAACTGCCAAAATGAAAACAGATACAAAAGAAACTTC AGGAAGTTTGGAGACTATAAATGAGCCTACTCCCCAGCCTATGCCTTTTCCACCCAGCATAATCAGATCA GGATCCCCGAAATTGGATCCTTCTGAAGTCTACCTGAAATCTAAGACTTTATATGAAGATAAACCACCCA ACACCAAAGACCTAGATGTCTTTCTTCGGAAACAAGAATCGGGGTTTGGCTTCAGGGTGCTGGGAGGAGA TGGACCTGACCAGTCTATATATATTGGGGCCATCATTCCCCTGGGAGCAGCGGAAAAGGATGGTAGGCTC CGTGCTGCTGATGAATTGATGTGTATTGATGGGATTCCTGTTAAAGGGAAATCACATAAGCAAGTCTTGG ACTTAATGACCACTGCTGCTCGAAATGGCCACGTGTTACTCACTGTGAGGAGGAAGATCTTCTATGGAGA GAAACAACCCGAGGATGAAAGCCCTCAAGCCTTCTCACAGAGTGGATCTCCTCGCCTAAATCGGACAGAG CTTCCAACCAGGTCTGCCCCACAGGAGTCCTATGATGTTATCTTACAGAGGAAAGAAAATGAAGGGTTTG GTTTTGTCATCCTCACCTCTAAAAGCAAGCCGCCCCCAGGAGTTATTCCTCATAAAATTGGCCGGGTCAT AGATGGAAGTCCAGCTGATCGTTGTGGAAGACTGAAAGTTGGAGACCACATCTCTGCGGTGAATGGGCAG TCCATTGTTGACCTGTCCCATGATAATATTGTTCAGCTCATCAAAGATGCTGGGGTCACCGTCACGCTGA CAGTGGTTGCTGAAGAAGAGCATCATGGTCCACCATCAGGAACAAACTCAGCCAGGCAGAGCCCAGCACT GCAGCACAGACCCATGGGACAGGCGCAGGCCACCCACATACCTGGGGACAGAACTGCCCTAGAAGGTGAA GTTGGGAAAGATGTCTGCAGCTCTTACAGACACTCCTGGTCTGACCACAAGCACCTTGCACAGCCGGACA CCGCGGTGATTTCCGTGGTGGGCAGTCGGCACAGTCAGAGCCTTGGGTGTTACCCTGTGGAGCTGGAGAG AGGCCCTCGAGGCTTTGGGTTCAGCCTCCGAGGAGGGAAGGAGTACAACATGGGGCTGTTCATCCTTCGC CTTGCTGAGGACGGGCCTGCCATCAAAGACGGCAGAATTCACGTTGGTGACCAGATCGTGGAAATCAATG GGGAACCTACACAAGGCATCACACACACGCGAGCCATTGAGCTCATTCAGGCTGGCGGGAATAAAGTCCT CCTCCTTCTGAGGCCAGGAACTGGCTTGATACCTGATCATGGTGATTGGGATATTTATAGTCCTTCATCT TCCAATGTCATTTATGATGAACAGCCACCACCTCTCCCCTCTTCACATTCTGCTGCCACATTTGAAGAGT CTCATGTGCCAGTAACTGAAGACTCTCTGATTAGAGTTCAGACATGTGAAAAGGCAGAAGAATTAAAGGA CACTGTACAAGAAAAGAAAAGCACTTTAAATGGAAGCCAGCCTGAGATGAAGTATCAGTCTGTCCAGAAA AATGTGAGTAAGAAGGATCCATCCAGAAGTCATGGGCATGGTGACAAGAATCTACCTAAAGGAGAAAATG GTGTTACACGGAGAGGTAGATCTGCTAGTCCCAAAAAGTCAGTTAATCGACATTCAGAGGAACATCTGGA AAAGATTCCCAGGCCCCTTAGAAGTGACCCCAAAGGAAAATCTAGAGACCGGTCACTCAGCCCTAGGAAA GGAGAAAATAAAGGTCAGGTCACCATTAAGGCGGGCTCTGGACAAGATCCCTGTAGAAAAGACAGAGGAC GGTCTTCTAGCCCCAGGAAGCAGCAAAAGATTGGAGGCAACAGCCTGTCAAACACTGAAGGCAAATTATC AGAGGCTGGGAGCCGAAGAGCAGCAGGCCTCTCTAGTGACAGCCCTGAACAGCTCCCAGAGGGGAAAGAA AAGTCAGGGGTCAGCAGGAAAGATCTGAAGCTCAGTCAGCTGGGGAAAAACAGAACAAGGTCTCCAGAGA AAAGGAGCAGCAAAGTTGATGAAGCGTCTCTCCCATCCAAAAAGACTAGTGACACTGCTAGTAGGGTAGT GTCTGAAAAGGAGAAAGGAAGGAAGCCAGGCACAGGAGAAAGAAGTAGAGACAAAACAGGAGAAAGCGTC CAGACCTCAGCAAAGCCGCTCACGCAGGAAGCGGGAGAGAAGATGGCATTAAGCAAAGCATCAGAGGTCA CTGACAGAGGCAAGGAGCGTGCAGGGGGCGCACCTGAGAGTAGCTCTCCAGTCAAGAAAGCACCCATCAC TCCAGGACCCTGGAGGGTGCCACGTGCAAATAAAGTCACAGGCACTGCTGGCATGGCTGACAAACAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213790 representing NM_139084 Red=Cloning site Green=Tags(s) MSKTLKKKKHWLSKVQECAVSWAGPPGDLGAEIRGGAERGEFPYLGRLRDEPGGGGGTCCVVSGKAPSPG DVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNL YLITIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQV LFDNEFDTESQRKRTTSVSKMERMDSSLPEEEEDEDKEAVNGSGSMETREMHSESSDCWMKTVPSYNQTN RSMDFRNYMMRDENLEPLPKNWEMAYTDTGTIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEK IEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQIGQAETHSAKTDVERAHFTRDPSQLKGVLVRASLKKST MGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKMAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVN LTLCRGYALPDDSEDPVVDIVAATPVINGQSLAKGEACMSTQDFKLGAMVLDQNGKSGKLLSSDRLNGPS DSNEQRASLASSGSSQPELVTIPLVKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNV QNLTHLQVVEVLKQFPVGADVPLLILRGGPCSPTKTAKMKTDTKETSGSLETINEPTPQPMPFPPSIIRS GSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRL RAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDESPQAFSQSGSPRLNRTE LPTRSAPQESYDVILQRKENEGFGFVILTSKSKPPPGVIPHKIGRVIDGSPADRCGRLKVGDHISAVNGQ SIVDLSHDNIVQLIKDAGVTVTLTVVAEEEHHGPPSGTNSARQSPALQHRPMGQAQATHIPGDRTALEGE VGKDVCSSYRHSWSDHKHLAQPDTAVISVVGSRHSQSLGCYPVELERGPRGFGFSLRGGKEYNMGLFILR LAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGDWDIYSPSS SNVIYDEQPPPLPSSHSAATFEESHVPVTEDSLIRVQTCEKAEELKDTVQEKKSTLNGSQPEMKYQSVQK NVSKKDPSRSHGHGDKNLPKGENGVTRRGRSASPKKSVNRHSEEHLEKIPRPLRSDPKGKSRDRSLSPRK GENKGQVTIKAGSGQDPCRKDRGRSSSPRKQQKIGGNSLSNTEGKLSEAGSRRAAGLSSDSPEQLPEGKE KSGVSRKDLKLSQLGKNRTRSPEKRSSKVDEASLPSKKTSDTASRVVSEKEKGRKPGTGERSRDKTGESV QTSAKPLTQEAGEKMALSKASEVTDRGKERAGGAPESSSPVKKAPITPGPWRVPRANKVTGTAGMADKQL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_139084
ORF Size	4410 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_139084.2, NP_620784.2
RefSeq Size	5863 bp
RefSeq ORF	4413 bp
Locus ID	245903
UniProt ID	Q9JK71
MW	160.5 kDa
Gene Summary	associates with tyrosine-phosphorylated proteins to link the receptor with its substrate in the plasma membrane [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.