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Fem1a (NM_001025706) Rat Tagged ORF Clone
CAT＃: RR214192
- TrueORF®
Fem1a (Myc-DDK-tagged ORF) - Rat feminization 1 homolog a (C. elegans) (Fem1a), (10 ug)
"NM_001025706" in other vectors (3)
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CNY 8,460.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC114562 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214192 representing NM_001025706
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTACACACAGCGGTGTACAATGCGGCGCACGACGGCAAGCTGCTGCTGCTTCAGAAGCTGCTGG CCGGCCGCGGGCGTGAGGAGATAGAGGAGCTGCTGGGTGAGGTGGCCGGCGGTGGCACACCACTGCTAAT CGCGGCGCGCCGTGGACACCTGGACGTGGTGGAGTACCTGGTGGACAACTGCGGCGCCAGTGTGGAGGCG AGCGGCTCCGTACACTTCGATGGCGAGACTATTGAGGGCGCGCCGCCGCTATGGGCAGCGTCAGCAGCGG GTCACTTAGCTGTGGTGCGTAGCCTTCTGCGCCGAGGCGCCTCGGTCAACCGCACTACGCGCACCAACTC TACGCCGCTACGCGCCGCGTGCTTTGACGGCCACCTGGACGTGGTGCGCTACCTGGTGGGCGAGCACAAG GCGGACCTAGAGGTAGCCAACCGCCACGGCCACACGTGCCTTATGATATCATGCTACAAGGGCCACCGCG AGATTGCGCGCTACCTCCTGGAACGGGGCGCGCAGGTGAATCGTCGCAGCGCCAAGGGCAACACCGCCCT TCACGACTGTGCGGAATCTGGCAGCCTGGAGATTCTGCAGCTGCTGCTGGGCTGTCACGCGCGTATGGAA CGCGACGGCTATGGCATGACCCCGCTACTGGCTGCCAGCGTCACCGGGCACACCAACATCGTGGAGTACC TCATCCAGGAACAGCCCGGCCACGGGCAGCTCTCAGGGACAGAGCTTCCCGGAGAAGGCTCCTCCCAGAT GGCAGGAAACCACTGTTCCACCCCAGAGGAAGCAGAACCGTATGAGAGCTGCTGCCCTACCAGCCGGGAA GCGGCTGTGGAAGCTTTGGAGCTGCTAGGTGCTACCTATGTCGATAAGAAAAGGGATCTGCTTGGAGCCC TGAAGCATTGGAGACGGGCAATGGAACTCCGCCACCAGGGTGGGGACTACCTTCCTAAGCCTGAACCCCA GCAACTGGTTCTAGCCTATGACTATTCCAGGGAGGTGAGCACGCCCCAAGAGTTGGAAGCCCTCATCACA GATCCTGATGAGATGCGGATGCAGGCACTGCTGATACGGGAGAGGATCCTGGGCCCCTCGCATCCTGACA CTTCATATTACATAAGATACCGGGGTGCTGTCTATGCAGACTCTGGAAATTTCGAGCGCTGTATCCGTCT GTGGAAGTATGCCTTGGACATGCAACAGAACAACCTGGAGCCCCTGAGCCCCATGACCGCCAGCAGCTTC CTGTCGTTTGCAGAGCTCTTCTCCTACGTGCTGCAGGACCGCTCAGCCAAGGGCAACCTAGGCATGCAGC TTGGCTTTGCTGACCTCATGGGTGTGCTCAGCAAAGGGGTTCGGGAAGTGGAGCGGGCTCTACAGCTGCC CAAGGAACCGGGTGACTCTGCACAGTTCACCAAAGCCATTGCCATCATCCTCCACCTTCTGTATCTGCTG GAGAAGGTGGAGTGCACCCCCAGCCAGGAACACCTCAAGCACCAGACAGTCTACCGCCTGCTCAAGTGTG CCCCTCGAGGTAAGAACGGCTTCACCCCACTGCACATGGCAGTGGATAAGGAGACCACCAACGTGGGCCG GTACCGTGTGGGCATCTTCCCGTCGTTGCAGGTGGTGAAGGTACTGTTGGACTGCGGAGCAGACCCTGAC AGCAGGGACTTCGACAACAACACACCACTGCATGTCGCTGCCCAGAACAATTGCCCTGCCATCATGGATG CCCTCATCGAAGCTGGAGCCCACATGGATGCCACCAATGCCTTCAAGAAGACTGCCTACGAGCTGCTGGA CTCGAAGCTGTTAGCCAAGAGCACCATGCAGCCCTTCAATTATGTGACACTCCAGTGCCTAGCTGCTCGT GCCCTGGACAGGAACAAGGTCCCTTATAAGGGCTTTATCCCAGAGGAGCTGGAGGCCTTCATCCAGTTAC AC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214192 representing NM_001025706
Red=Cloning site Green=Tags(s) MDLHTAVYNAAHDGKLLLLQKLLAGRGREEIEELLGEVAGGGTPLLIAARRGHLDVVEYLVDNCGASVEA SGSVHFDGETIEGAPPLWAASAAGHLAVVRSLLRRGASVNRTTRTNSTPLRAACFDGHLDVVRYLVGEHK ADLEVANRHGHTCLMISCYKGHREIARYLLERGAQVNRRSAKGNTALHDCAESGSLEILQLLLGCHARME RDGYGMTPLLAASVTGHTNIVEYLIQEQPGHGQLSGTELPGEGSSQMAGNHCSTPEEAEPYESCCPTSRE AAVEALELLGATYVDKKRDLLGALKHWRRAMELRHQGGDYLPKPEPQQLVLAYDYSREVSTPQELEALIT DPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFERCIRLWKYALDMQQNNLEPLSPMTASSF LSFAELFSYVLQDRSAKGNLGMQLGFADLMGVLSKGVREVERALQLPKEPGDSAQFTKAIAIILHLLYLL EKVECTPSQEHLKHQTVYRLLKCAPRGKNGFTPLHMAVDKETTNVGRYRVGIFPSLQVVKVLLDCGADPD SRDFDNNTPLHVAAQNNCPAIMDALIEAGAHMDATNAFKKTAYELLDSKLLAKSTMQPFNYVTLQCLAAR ALDRNKVPYKGFIPEELEAFIQLH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025706 |
| ORF Size | 1962 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025706.1, NP_001020877.1 |
| RefSeq Size | 2222 bp |
| RefSeq ORF | 1965 bp |
| Locus ID | 316131 |
| UniProt ID | Q4V890 |
| MW | 72.1 kDa |
| Gene Summary | Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. May participate in antiinflammatory signaling via its interaction with PTGER4 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214192 | Fem1a (untagged ORF) - Rat feminization 1 homolog a (C. elegans) (Fem1a), (10 ug) |
CNY 8,460.00 |
|
| RR214192L3 | Lenti ORF clone of Fem1a (Myc-DDK-tagged ORF) - Rat feminization 1 homolog a (C. elegans) (Fem1a), (10 ug) |
CNY 10,260.00 |
|
| RR214192L4 | Lenti ORF clone of Fem1a (mGFP-tagged ORF) - Rat feminization 1 homolog a (C. elegans) (Fem1a), (10 ug) |
CNY 11,210.00 |
