Gne (NM_053765) Rat Tagged ORF Clone

CAT＃: RR214376

  • TrueORF®

Gne (Myc-DDK-tagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)

Datasheet
SDS
Plasmid Map

  "NM_053765" in other vectors (3)

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CNY 9,220.00

货期*
6周
规格
    • 10 ug

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

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【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Uae1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR214376 representing NM_053765
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGAACGGGAATAACCGGAAGCTTCGGGTTTGCGTTGCCACCTGCAACCGAGCCGATTACTCCA
AACTGGCCCCCATCATGTTCGGCATTAAGACGGAGCCTGCGTTCTTCGAGCTCGACGTGGTGGTGCTGGG
CTCTCACCTGATCGACGACTACGGAAACACATACCGCATGATTGAGCAGGACGACTTTGACATCAACACC
AGGCTACACACGATTGTTAGAGGGGAAGACGAAGCAGCCATGGTAGAGTCAGTGGGCCTAGCGCTAGTGA
AGCTACCGGATGTCCTCAACCGCCTGAAGCCTGACATCATGATTGTTCACGGAGACCGATTTGACGCCCT
CGCTCTGGCTACATCTGCTGCCCTGATGAACATCCGCATCCTTCACATTGAAGGAGGAGAGGTCAGCGGG
ACTATTGATGACTCTATCAGACACGCCATAACAAAACTGGCTCACTACCACGTGTGCTGCACCAGGAGTG
CAGAGCAACACCTGATCTCCATGTGTGAGGACCACGACCGCATCCTTTTGGCTGGCTGCCCTTCCTATGA
CAAACTGCTCTCAGCCAAGAATAAAGACTATATGAGCATCATTCGGATGTGGCTAGGTGATGATGTAAAA
TGTAAAGATTACATTGTTGCCCTGCAACACCCGGTGACCACCGACATTAAGCATTCCATAAAGATGTTCG
AACTGACACTGGATGCTCTTATCTCATTTAACAAGAGGACCCTAGTTCTGTTTCCAAATATCGATGCAGG
CAGCAAGGAGATGGTTCGAGTGATGCGGAAGAAGGGCATCGAGCATCACCCCAATTTCCGCGCAGTCAAG
CACGTCCCGTTTGACCAGTTCATTCAGCTGGTCGCCCACGCTGGCTGCATGATTGGGAATAGCAGCTGTG
GAGTGCGTGAGGTTGGCGCCTTTGGAACCCCTGTGATCAACCTGGGCACGCGGCAGATAGGAAGAGAAAC
GGGGGAGAATGTTCTTCATGTCCGGGATGCTGACACCCAAGACAAAATATTACAAGCACTACACCTCCAG
TTCGGTAAACAGTACCCTTGCTCAAAGATATATGGGGATGGAAATGCTGTTCCAAGGATTTTAAAGTTTC
TCAAATCCATCGACCTTCAAGAGCCACTACAGAAGAAATTCTGCTTCCCTCCCGTGAAGGAGAACATCTC
TCAGGATATTGACCATATCCTCGAAACTCTGAGTGCCTTGGCTGTTGATCTCGGGGGGACGAATCTGAGA
GTGGCGATAGTTAGCATGAAGGGTGAAATAGTTAAGAAGTACACCCAGTTCAATCCTAAAACCTATGAGG
AAAGGATTAGTCTAATCCTGCAGATGTGTGTGGAAGCGGCAGCAGAAGCCGTGAAGCTCAATTGCAGAAT
TCTGGGAGTAGGCATCTCCACAGGTGGCCGTGTGAATCCCCAGGAAGGAGTTGTGCTGCACTCGACCAAG
CTGATACAGGAGTGGAACTCTGTGGACCTCAGGACACCACTCTCCGACACCCTGCATCTCCCCGTGTGGG
TGGACAACGACGGCAACTGCGCTGCCATGGCGGAGAGGAAGTTTGGCCAAGGAAAAGGACAGGAGAACTT
TGTGACGCTCATCACAGGGACAGGGATCGGTGGGGGAATCATCCACCAGCACGAGCTGATCCACGGCAGC
TCCTTCTGTGCGGCAGAGCTTGGCCACCTCGTGGTGTCTCTGGATGGTCCTGACTGCTCCTGTGGAAGCC
ATGGGTGCATTGAAGCCTACGCCTCTGGAATGGCCTTGCAGAGGGAAGCAAAGAAGCTCCACGACGAGGA
CCTGCTCTTGGTGGAAGGGATGTCAGTGCCAAAAGACGAAGCTGTGGGCGCCCTCCATCTCATCCAAGCC
GCCAAGCTGGGCAACGTGAAGGCCCAGAGCATCTTACGGACAGCTGGAACTGCTTTGGGACTCGGAGTTG
TGAATATCCTCCACACTATGAATCCTTCCCTGGTGATCCTGTCTGGAGTCCTGGCTAGTCACTACATCCA
CATTGTGAGGGACGTCATCCGCCAGCAAGCCCTGTCCTCCGTGCAGGATGTGGATGTAGTGGTTTCAGAC
TTGGTTGACCCGGCCCTGCTTGGTGCGGCCAGCATGGTTCTGGACTACACGACCCGCAGGATCCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RR214376 representing NM_053765
Red=Cloning site Green=Tags(s)

MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPAFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINT
RLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSG
TIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVK
CKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVK
HVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQ
FGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLR
VAIVSMKGEIVKKYTQFNPKTYEERISLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPQEGVVLHSTK
LIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAAMAERKFGQGKGQENFVTLITGTGIGGGIIHQHELIHGS
SFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQA
AKLGNVKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVRDVIRQQALSSVQDVDVVVSD
LVDPALLGAASMVLDYTTRRIH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_053765
ORF Size 2166 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_053765.2, NP_446217.1
RefSeq Size 2843 bp
RefSeq ORF 2169 bp
Locus ID 114711
UniProt ID O35826
MW 79.2 kDa
Gene Summary bifunctional enzyme, catalyzes the first two steps of biosynthesis of Neu5Ac (UDP-GlcNAc 2-epimerase and ManNAc kinase), precursor of sialic acids [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
RN214376 Gne (untagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)
    • 10 ug

CNY 9,220.00

RR214376L3 Lenti ORF clone of Gne (Myc-DDK-tagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)
    • 10 ug

CNY 11,020.00

RR214376L4 Lenti ORF clone of Gne (mGFP-tagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)
    • 10 ug

CNY 12,160.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

其它Gne产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
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RR214376L3

Lenti ORF clone of Gne (Myc-DDK-tagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)

    • 10 ug

CNY 11,020.00

RN214376

Gne (untagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)

    • 10 ug

CNY 9,220.00

RR214376L4V

Lenti ORF particles, Gne (GFP-tagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), 200ul, >10^7 TU/mL

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CNY 13,395.00 暂停销售

RR214376L4

Lenti ORF clone of Gne (mGFP-tagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)

    • 10 ug

CNY 12,160.00

RR214376L3V

Lenti ORF particles, Gne (Myc-DDK-tagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), 200ul, >10^7 TU/mL

    • 200 ul

CNY 12,046.00 暂停销售

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