Edc4 (NM_001033068) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	LRRG00115; Nrn1l; RGD1309136
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214641 representing NM_001033068 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCCTGCGCGAGCATCGACATTGAGGACGCCACGCAGCATCTGCGGGACATCCTCAAACTGGACC GGCCCGCCGGAGGCTCCAATGTCGAAAGCCAAAGGCCATCCAGTGCCTACAATGGAGACCTCAATGGGCT CCTGGTCCCAGATCCGCTCAGTTCAGGAGATGGTAACTCGACAAGCAAACCTGGTATACGGACCATGCCA CCCATTAATCTGCAGGAAAAGCAGGTCATTTGCCTCTCTGGAGATGACAGCTCCACGTGCATTGGGATTT TGGCCAAGGAGGTAGAAATTGTGGCCAGCAGTGATTCTAGCATTTCCAGCAAGGCGCGAGGGAGCAACAA GGTGAAAATCCAGCCTGTAGCCAAGTATGACTGGGAGCAGAAATACTACTATGGCAACCTGATTGCAGTG TCTAACTCCTTCTTGGCATACGCCATCAGGGCTGCCAACAATGGCTCAGCCATGGTCCGAGTGATCAGTG TGAGCACTTCGGAGCGGACCCTGCTCAAGGGCTTCACAGGCAGTGTGGCTGACCTTGCATTTGCACACCT CAACTCTCCACAGCTTGCCTGCCTGGACGAGGCCGGCGATCTCTTTGTGTGGCGACTGGCTCTGGTTAAG GGCAAAATTCAAGAAGAGATCTTAGTCCATATCCGGCAACCAGAGGGCACACCACTGAACCACTTCCGAA GGATCATCTGGTGCCCTTTCATCCCTGAGGAAAGCGAGGACTGTTGTGAGGAGAGTAGCCCAACGGTGGC CCTGCTGCATGAAGACCGGGCTGAGGTGTGGGACTTGGATATGCTTCGCTCCAGCCACAGCACATGGCCT GTGGATGTCAGCCAAATCAAGCAGGGCTTTATCGTGGTAAAAGGCCATAGCACGTGCCTAAGTGAAGGAG CCCTTTCCCCTGATGGGACTGTGCTGGCCACCGCAAGCCATGATGGCTTTGTCAAGTTCTGGCAGATCTA TATTGAAGGTCAGGATGAGCCAAGGTGTCTGCATGAATGGAAACCTCATGATGGGCGGCCTCTTTCTTGC CTCCTGTTCTGTGATAACCATAAGAAACAGGATCCTGAGGTCCCATTCTGGAGGTTCCTCATTACTGGCG CTGACCAGAATCGAGAGCTGAAGATGTGGTGCACAGTGTCCTGGACCTGCCTGCAAACCATTCGCTTCTC CCCAGATATCTTCAGTTCAGTGAGTGTGCCTCCCAGTCTCAAAGTTTGCTTGGACCTCTCAGCAGAATAT TTGATTCTTAGTGATGTGCAACGAAAGGTCCTCTATGTGATGGAGCTGCTGCAGAACCAGGACGAGGGCC GTGCTTGCTTCAGTTCCATCTCTGAGTTCCTGCTCACCCACCCTGTGCTGAGCTTTGGCATCCAGGTTGT GAGTCGCTGCCGGCTGCGGCACACTGAGGTGCTGCCTGCTGAGGAGGAGAGTGACAGTCTGGGGACTGAG AGTTCCCATGGAGCTGGTACCTTGGAATCTGCAGCTGGTGTGCTTATCAAGCTCTTTTGTGTGCACACTA AGGCACTCCAAGATGTGCAGATCCGCTTCCAGCCACAGCTGAACCCTGATGTGGTGGCCCCACTCTCTAC CCACACTGCTCATGAGGACTTCACATTTGGAGAGTCTCGACCTGAACTAGGCTCTGAGGGGCTAGCTTCA GCTCCTCATGGTTCCCAGCCTGACCTCCGACGCATTGTGGAGCTGCCTGCACCTGCAGACTTCCTCAGTC TGAGCAGTGAGACCAAGCCTAAGTTGATGACACCTGATGCTTTCATGACACCTACCGCCTCCCTGCAGCA GATCTCTGCATCCCCTAGTAGTAGCAGCAGCAGTAGCAGCAGCAGCAGTAGCAGCAGCAGCAGTAGCAGC AGCAGCTCTCTAACAGCTGTGTCTGCTGTGAGCAGCTCCTCAGCCATGGACCCCTCCTTGCCCAGTAGGC CACCCGAGGAACTGACCTTGAGCCCCAAACTGCAGCTAGATGGCAGCCTGACTATAAGCAGCAGCAGCAG CCTGCAGGCAAGCCCTCGGAGCCTTCTTCCGGGCCTGCTCCCAGGCCCAGCTGACAAACTAATTCCCAAG GGACCTGGTCAGGTATCTTCTGGTACTTCTGCACTATCCTTGGATTTGCAGGAAGTGGAACCATTAGGGC TACCCCAGGCCTCCCCAAGTCGCACACGTTCTCCTGATGTGATCTCCTCAGCATCCACTGCCCTGTCCCA GGACATCCCTGAAATTGCATCTGAGGCCCTGTCCCGTGGCTTTGGCTCCTCTGTACCTGAGGGCCTCATT GAGCCAGACAGCATGGCCTCAGCTGCCTCAGCACTACACCTACTGTCTCCTCGGCCCAGGCAAGGCCCTG AGCTTAGTTCTCAGCTTGGTCTAGATGGAGGCCCTGGGGATGGGGATAGGCATAGTACCCCTTCCCTACT GGAAGCAGCCTTGACCCAGGAAGTTGCAACCTCTGACAGTCAAGTCTGGCCTACAGCACCTGACATTACT CGTGAGACCTGTAGCACACTAACAGAAAGCCCCAGGAATGGCCTCCAGGAAAAGCACAAAAGCCTGGCCT TCCACAGGCCACCTTATCACCTGCTGCAGCAACATGACAGTCAGGACACAAGTGCTGAGCAAAGTGACCA TGACGATGAGGTTGCCAGCCTTGCCTCTGCTTCAGGAGGTTTTGGCAGCAAAATTCCTACTCCACGGCTG CCTGCCAAGGATTGGAAAACCAAGGGATCCCCTAGGACTTCACCTAAGCTCAAGAGGAAAAGCAAGAAGG ATGATGGGGATTCAGCTGTGGGATCTCGGCTCACCGAGCACCAGGTGGTAGAGCCCCCTGAAGACTGGCC AGCACTAATTTGGCAGCAGCAAAGAGAGCTGGCAGAACTATGGCACAACCAAGAAGAGCTGCTACAGCGT CTTTGTGCCCAACTTGAAGGTCTTCAGAGCACTGTCACAGACCATGTAGAACGTGCCCTGGAGACACGGC ATGAGCAAGAGCAACGGCGACTGGAGCGGGCACTGGCTGAGGGGCAGCAACGGGGTGGGCAGCTACAGGA GCAGCTGACACAGCAGCTATCCCAGGCTTTGTCTTCAGCTGTGGCTGGGCGGCTAGAGCGCAGCATAAGG GATGAAATCAAGAAAACAGTTCCTCCATGTGTCTCCAGAAGTCTGGAGCCTGTGGCAGGCCAACTAAGCA ACTCAGTGGCTACCAAGCTTACAGCTGTGGAAGGCAGCATGAAAGAGAATATCTCTAAGCTACTCAAGTC CAAGAACTTAACAGATGCCATTGCCCGCGCAGCTGCAGATACGCTACAGGGACCAATGCAGGCTGCCTAC CGAGAAGCCTTCCAGAGTGTGGTGCTGCCGGCTTTTGAGAAGAGTTGTCAGGCTATGTTCCAGCAAATCA ATGACAGCTTCAGGCTGGGCACTCAGGAATATTTGCAGCAGCTGGACAGTCACATGAAGAGCCGAAAGGC ACGTGAACAAGAAGCTAGGGAGCCTGTGTTGGCCCAGCTTCGGGGCCTGGTCAGCACACTGCAGAATGCC ACTGAGCAGATGGCAGCCACTGTGTCTAGCAGTGTTCGGGCCGAGGTGCAACACCAGCTGCATGTGGCTG TGGGCAGCTTGCAGGAATCAATCTTAGCACAAGTACAACGCATTGTCAAGGGTGAAGTGAGTGTGGCACT GAAGGAGCAGCAGGCCACTGTCACTTCCAGCATCATGCAGGCTATGCGCTCAGCTGCTGGCACACCTGTC CCCTCTGCCCACCTTGACTGCCAGGCCCAACAAGCCCATATCTTGCAGTTACTGCAGCAGGGCCACCTCA ATCAGGCCTTCCAGCAGGCTCTGACTGCTGCTGATCTCAACCTGGTGCTGTATGTGTGTGAAACTGTGGA CCCAGCCCAGGTTTTTGGGCAGCCGCCCTGTCCACTGTCCCAGCCCGTTCTCCTTTCCCTAATCCAGCAG CTAGCATCTGACCTTGGAACTAGAAGTGACCTCAAGCTCAGCTACTTGGAAGAGGCTGTGATGCACCTGG ACCACAGCGATCCCATCACTCGAGACCACATGGGCTCTGTCATGGCTCAGGTGCGCCAGAAGCTCTTCCA GTTCCTGCAGGCTGATCCACACAACTCACTTGGCAAAGCTGCCCGCCGCCTCAGCCTAATGTTACACGGC CTTGTGACCCCTAGCCTCCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214641 representing NM_001033068 Red=Cloning site Green=Tags(s) MASCASIDIEDATQHLRDILKLDRPAGGSNVESQRPSSAYNGDLNGLLVPDPLSSGDGNSTSKPGIRTMP PINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAV SNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGDLFVWRLALVK GKIQEEILVHIRQPEGTPLNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHSTWP VDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGFVKFWQIYIEGQDEPRCLHEWKPHDGRPLSC LLFCDNHKKQDPEVPFWRFLITGADQNRELKMWCTVSWTCLQTIRFSPDIFSSVSVPPSLKVCLDLSAEY LILSDVQRKVLYVMELLQNQDEGRACFSSISEFLLTHPVLSFGIQVVSRCRLRHTEVLPAEEESDSLGTE SSHGAGTLESAAGVLIKLFCVHTKALQDVQIRFQPQLNPDVVAPLSTHTAHEDFTFGESRPELGSEGLAS APHGSQPDLRRIVELPAPADFLSLSSETKPKLMTPDAFMTPTASLQQISASPSSSSSSSSSSSSSSSSSS SSSLTAVSAVSSSSAMDPSLPSRPPEELTLSPKLQLDGSLTISSSSSLQASPRSLLPGLLPGPADKLIPK GPGQVSSGTSALSLDLQEVEPLGLPQASPSRTRSPDVISSASTALSQDIPEIASEALSRGFGSSVPEGLI EPDSMASAASALHLLSPRPRQGPELSSQLGLDGGPGDGDRHSTPSLLEAALTQEVATSDSQVWPTAPDIT RETCSTLTESPRNGLQEKHKSLAFHRPPYHLLQQHDSQDTSAEQSDHDDEVASLASASGGFGSKIPTPRL PAKDWKTKGSPRTSPKLKRKSKKDDGDSAVGSRLTEHQVVEPPEDWPALIWQQQRELAELWHNQEELLQR LCAQLEGLQSTVTDHVERALETRHEQEQRRLERALAEGQQRGGQLQEQLTQQLSQALSSAVAGRLERSIR DEIKKTVPPCVSRSLEPVAGQLSNSVATKLTAVEGSMKENISKLLKSKNLTDAIARAAADTLQGPMQAAY REAFQSVVLPAFEKSCQAMFQQINDSFRLGTQEYLQQLDSHMKSRKAREQEAREPVLAQLRGLVSTLQNA TEQMAATVSSSVRAEVQHQLHVAVGSLQESILAQVQRIVKGEVSVALKEQQATVTSSIMQAMRSAAGTPV PSAHLDCQAQQAHILQLLQQGHLNQAFQQALTAADLNLVLYVCETVDPAQVFGQPPCPLSQPVLLSLIQQ LASDLGTRSDLKLSYLEEAVMHLDHSDPITRDHMGSVMAQVRQKLFQFLQADPHNSLGKAARRLSLMLHG LVTPSLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001033068
ORF Size	4221 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001033068.3, NP_001028240.3
RefSeq Size	4741 bp
RefSeq ORF	4224 bp
Locus ID	361399
UniProt ID	Q3ZAV8
MW	152.6 kDa
Gene Summary	In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).[UniProtKB/Swiss-Prot Function]

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