Abcc4 (NM_133411) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mrp4; RGD1565953
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214716 representing NM_133411 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCGGTGCACACCGAGGTGAAACCCAACCCGCTGCAGGACGCCAACCTCTGCTCGCGCTTGTTCT TCTGGTGGCTCAACCCGTTGTTTAAAGCTGGACATAAGCGGAGATTGGAAGAAGATGACATGTTTTCAGT GCTTCCAGAAGATCGCTCAAAGCACCTTGGAGAGGAGTTGCAAGGGTACTGGGATAAAGAAGTTCTGCGA GCCAAGAAGGACGCTCGGAAGCCTTCCTTAACGAAGGCAATCGTGAAGTGTTACTGGAAATCTTACCTGA TTTTGGGAATTTTTACGTTAATTGAGGAGACCACCCGAGTAGTTCAGCCCATATTTTTAGGGAAAATTAT TGATTATTTTGAGAAGTATGACTCTGACGACTCGGCCGCTTTGCACACAGCTTACGGCTACGCGGCGGTG CTGTCGCTGTGCACGCTCATCCTGGCCATACTACATCACTTGTATTTCTACCACGTGCAGTGCGCCGGGA TGAGGATACGGGTCGCCATGTGCCACATGATTTACCGGAAGGCTCTCCGGCTAAGTAACTCGGCCATGGG GAAGACAACCACGGGCCAGATAGTCAACCTGCTGTCCAACGACGTGAACAAGTTTGACCAGGTGACCATC TTCTTGCACTTCCTGTGGGCGGGGCCGCTGCAAGCCATAGGGGTAACCATCCTTCTCTGGGTGGAAATCG GAATCTCCTGTCTGGCGGGCCTGGCCATTCTGGTTATTCTGCTGCCTCTGCAAAGCTGCATCGGGAAACT GTTCTCGTCACTGCGGAGTAAAACAGCGGCTTTCACGGATGCCAGGTTCCGGACCATGAATGAAGTGATA ACGGGCATGAGGATAATAAAGATGTACGCGTGGGAGAAGTCATTCGCCGACCTCATAACCAATCTGAGAA AGAAGGAAATTTCTAAGATTCTGGGCAGCTCCTACCTCAGAGGGATGAACATGGCGTCGTTTTTCATTGC AAACAAAGTCATCCTGTTCGTGACCTTCACCACCTATGTGCTGCTTGGCAATAAGATTACATCTAGCCAC GTGTTCGTGGCCATGACTCTGTATGGTGCTGTCCGGTTGACAGTTACCCTCTTCTTCCCATCAGCCATTG AGAGAGTGTCAGAGGCCGTCGTCAGCGTTCGGAGGATCAAGAACTTTCTGTTACTCGATGAACTGCCAGA GCGCAAAGCCCAGGAACCATCTGATGGCAAAGCCATCGTTCACGTGCAAGATTTTACCGCTTTCTGGGAC AAGGCATTAGACACTCCTACCCTGCAAGGTCTTTCCTTCACTGCCAGACCTGGTGAGTTGTTAGCTGTGG TCGGCCCAGTCGGAGCAGGCAAGTCGTCCCTGTTGAGCGCGGTGCTCGGTGAGCTGCCTCCCACCAGTGG GCTGGTCAGCGTGCACGGGAGGATCGCCTACGTTTCCCAGCAGCCCTGGGTGTTCTCAGGAACCGTGAGG AGCAATATTTTATTTGGGAGGAAATACGAGAAAGAACGCTACGAGAAAGTCATCAAGGCCTGTGCTCTGA AGAAGGACTTGCAACTTCTGGAGGACGGGGATCTGACTGTCATAGGCGACCGGGGAGCCACGCTGAGCGG AGGCCAGAAAGCACGGGTGAACTTGGCACGGGCAGTCTACCAGGATGCCGACATCTACCTCCTCGATGAT CCGCTCAGTGCTGTTGATGCAGAAGTGGGCAAGCACCTGTTCCAGCTGTGTATCTGCCAGACTTTGCACG AGAAGATCACCATTTTAGTGACTCACCAGTTACAGTACCTCAAAGCTGCAAGCCACATCCTCATACTAAA AGATGGCGAAATGGTGCAGAAGGGGACTTACACCGAGTTTCTGAAGTCTGGTGTAGATTTCGGTTCCCTG CTAAAGAAGGAAAATGAGGAAGCAGAGCCCTCCCCTGTCCCAGGAACACCGACACTCAGGAACCGAACCT TCTCCGAGGCCTCGATTTGGTCTCAACAGTCATCCAGACCCTCGTTGAAAGACGGGGTCCCAGATGCCCA AGATGCAGAGAATACTCAGGCAGCTCAACCCGAGGAGAGCCGTTCAGAAGGGAGAATCGGCTTCAAGGCC TACAAGAACTACTTCTCTGCAGGCGCATCCTGGTTTTTCATTATTTTCCTCGTTCTGCTTAACCTGATGG GTCAGGTTTTCTACGTTCTTCAGGACTGGTGGCTTTCCCACTGGGCGAATAGGCAAGGTGCCCTGAATGA CACCAAAAACGCGAACGGGAATGTAACCGGGACCCTCGATCTCAGCTGGTACTTAGGGATTTACACAGGT TTAACTGCGGTCACAGTCCTCTTTGGCATAGCGAGATCCCTGCTGGTGTTCTATGTCCTTGTGAATGCTT CCCAGACTCTGCACAACAGGATGTTCGAGTCGATCCTGAAGGCACCGGTGTTGTTCTTCGATAGAAATCC AATAGGAAGGATTTTAAATCGTTTCTCCAAGGACATCGGACACATGGACGACTTGCTTCCCCTAACGTTT CTAGACTTCATCCAGACCTTGCTCCTTGTTGTAAGTGTGATCGCCGTGGCGGCGGCCGTGATCCCTTGGA TCCTCATACCCCTGGTTCCACTCTCCATCATCTTTGTGGTCCTTCGGAGATATTTCTTAGAAACGTCGCG GGACGTCAAGCGCCTGGAGTCCACAACACGGAGCCCGGTATTCTCCCATTTATCATCCTCCCTCCAGGGA CTCTGGACCATCCGTGCCTATAAGGCTGAAGAGAGGTGTCAGGAGCTGTTTGATGCACACCAGGACTTGC ATTCAGAGGCTTGGTTCTTGTTCCTGACGACATCAAGATGGTTCGCCGTGCGTCTGGACGCCATCTGCGC CGTCTTTGTCATCGTCGTTGCCTTCGGGTCCCTGGTTCTGGCAAAGACCTTGGATGCTGGGCAGGTTGGC TTGGCCTTGTCCTACTCCCTCACGCTCATGGGGATGTTCCAGTGGTCTGTGAGACAGAGCGCCGAAGTAG AGAATATGATGATTTCTGTGGAGAGGGTGATTGAATACACGGACCTAGAGAAGGAGGCGCCTTGGGAATG CAGGAAGCGCCCACCCCCAGGCTGGCCCCACGAAGGAGTAATTGTCTTCGACAATGTGAACTTCACCTAC AGCTTGGACGGGCCCCTTGTTCTGAAGCACCTGACCGCACTCATCAAGTCACGAGAAAAGGTTGGAATTG TGGGAAGAACTGGAGCTGGAAAAAGTTCCCTCATCTCAGCCCTCTTCAGGTTGTCAGAACCTGAGGGGAA AATCTGGATTGATAAGATCTTGACGACTGAAATTGGACTTCACGATTTAAGGAAGAAAATGTCAATCATA CCTCAGGAACCTGTCCTGTTCACTGGAACCATGAGGAAGAACCTGGACCCCTTTAATGAGCACTCGGACG AGGAGCTGTGGAAAGCCCTAGAGGAGGTACAACTTAAAGAGGCCATAGAAGATCTTCCTGGTAAAATGGA CACTGAACTAGCAGAATCTGGATCCAATTTCAGTGTTGGACAGAGACAGTTAGTGTGCCTTGCGAGGGCA ATTCTGAAGAAAAACCGGATACTGATCATTGATGAAGCAACTGCAAATGTGGATCCGAGAACTGACGAGT TAATACAACAGAAAATCCGGGAGAAGTTTGCCCAGTGCACTGTTCTCACCATTGCTCACAGACTGAACAC CATCATTGACAGCGACAAGATAATGGTTTTGGATTCGGGAAGACTGAGAGAATATGATGAGCCGTATGTT TTGCTGCAGAATCCAGAGAGCCTCTTTTACAAGATGGTTCAGCAGCTGGGTAAGGGCGAAGCCGCTGCCC TCACCGAAACAGCAAAACAGGTGTACTTCAGACGGAATTATCCGGATATTGCATTCAGCAGCCCTGCGGT TATGAGCACCTCCAACGGACAGCCCTCCGCCTTAACGATATTTGAAACAGCATTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214716 representing NM_133411 Red=Cloning site Green=Tags(s) MLPVHTEVKPNPLQDANLCSRLFFWWLNPLFKAGHKRRLEEDDMFSVLPEDRSKHLGEELQGYWDKEVLR AKKDARKPSLTKAIVKCYWKSYLILGIFTLIEETTRVVQPIFLGKIIDYFEKYDSDDSAALHTAYGYAAV LSLCTLILAILHHLYFYHVQCAGMRIRVAMCHMIYRKALRLSNSAMGKTTTGQIVNLLSNDVNKFDQVTI FLHFLWAGPLQAIGVTILLWVEIGISCLAGLAILVILLPLQSCIGKLFSSLRSKTAAFTDARFRTMNEVI TGMRIIKMYAWEKSFADLITNLRKKEISKILGSSYLRGMNMASFFIANKVILFVTFTTYVLLGNKITSSH VFVAMTLYGAVRLTVTLFFPSAIERVSEAVVSVRRIKNFLLLDELPERKAQEPSDGKAIVHVQDFTAFWD KALDTPTLQGLSFTARPGELLAVVGPVGAGKSSLLSAVLGELPPTSGLVSVHGRIAYVSQQPWVFSGTVR SNILFGRKYEKERYEKVIKACALKKDLQLLEDGDLTVIGDRGATLSGGQKARVNLARAVYQDADIYLLDD PLSAVDAEVGKHLFQLCICQTLHEKITILVTHQLQYLKAASHILILKDGEMVQKGTYTEFLKSGVDFGSL LKKENEEAEPSPVPGTPTLRNRTFSEASIWSQQSSRPSLKDGVPDAQDAENTQAAQPEESRSEGRIGFKA YKNYFSAGASWFFIIFLVLLNLMGQVFYVLQDWWLSHWANRQGALNDTKNANGNVTGTLDLSWYLGIYTG LTAVTVLFGIARSLLVFYVLVNASQTLHNRMFESILKAPVLFFDRNPIGRILNRFSKDIGHMDDLLPLTF LDFIQTLLLVVSVIAVAAAVIPWILIPLVPLSIIFVVLRRYFLETSRDVKRLESTTRSPVFSHLSSSLQG LWTIRAYKAEERCQELFDAHQDLHSEAWFLFLTTSRWFAVRLDAICAVFVIVVAFGSLVLAKTLDAGQVG LALSYSLTLMGMFQWSVRQSAEVENMMISVERVIEYTDLEKEAPWECRKRPPPGWPHEGVIVFDNVNFTY SLDGPLVLKHLTALIKSREKVGIVGRTGAGKSSLISALFRLSEPEGKIWIDKILTTEIGLHDLRKKMSII PQEPVLFTGTMRKNLDPFNEHSDEELWKALEEVQLKEAIEDLPGKMDTELAESGSNFSVGQRQLVCLARA ILKKNRILIIDEATANVDPRTDELIQQKIREKFAQCTVLTIAHRLNTIIDSDKIMVLDSGRLREYDEPYV LLQNPESLFYKMVQQLGKGEAAALTETAKQVYFRRNYPDIAFSSPAVMSTSNGQPSALTIFETAL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133411
ORF Size	3975 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133411.1, NP_596902.1
RefSeq Size	4526 bp
RefSeq ORF	3978 bp
Locus ID	170924
UniProt ID	Q6QMG6
MW	148.9 kDa
Gene Summary	ATP-binding cassette (ABC) multiple drug resistance protein (MRP) that mediates reduced glutatione release from hepatocytes into blood by cotransport with monoanionic bile salts [RGD, Feb 2006]

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