Sec24b (NM_001106474) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR215085 representing NM_001106474 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCCCCGCCGGGTCCCCTCACCCGGCCGCCGGCGCCCGGATGCCGCCCAAGCTCGGCGGAGCAG TCTCTGGCCTGGCGCCGCCGCAGCAGAATGGTCCAGCACAGAGTCAAATGCAAGTTCCATCTGGGTATGG GCTGCCTCATCAAAACTATATGGCCCCCTCAGGACACTACTCTCAAGGACCTGGGAAAATGACCTCCTTG CCGTTGGATAACCAGTGTGAGAATTACTACTCTCGTCCATATACAGCACCAACACAGAATGTGGGGACTC CCAGCTCAGCAAACCAACCAGGAGCACAGCTCATGTATGGCAGAGGTCCTTCTGCCCCTCACATGGGGGC ATCCATGCCAGGACCTTTCCAAGGAGCTCCAGCATCGGCGTCCCATTCGTACCCGAGTGCCTCCCAGCCC TATTCCTCTCTTGGGAACCGCTACAGTAGTCCTGCCACATACTCCGCCACCGCTTCTGTTGCTTCTCAGG GATACCCCTCCACTTGTAGTCATTATCCCATCTCCACTGTGTCTAATGTCGTGTATCCTAACGTTTCATA CCCCTCGCTGCCTGCCAGTGAGCCGTATGGGCAGATGTTTACCTCACAGAGTGCTCCTCCGCCTGCCAGG CCACTTAAAGAGTCATACTCTGGCCCAAGCACAGCTGTTGCCTACCCATCGAGACCTCCGCCTCCACCTT CCCAGCACCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGAGTCATTCAGGATACAGTTCTCT ACCATGGTCAGGTCCAGGCCTTCCACCAGCCCAAGACAGTCTCATCCGAAACCAAATGGGATCCTTGGCT ATACCGAACAGCCACCCAGCAATTAACGAAAATGTCCAACCACCTAAGCCCAGCTCCGTAGTAGCCACAG TTTTGCCAGGACCCTCATCAACAAGAATGCCACCTGCCCCAAGTCACCCAGTTGGGCCTGTGCCGTCTGC TCCACCCCCCCCAGAGCAGATGCAGACAAAAGGCATGCAGTATGGTGACTATGTTAATAATCAAGCTAGC TCCACACCAACTCCCTTGTCATCAGCTTCCGATGATGAGGAAGAGGAGGATGAGGATGAGGAAGCAGGTG TTGACAGCTCTTCTACCACAAGCAGCGCTTCGCCCTTGCCCAACAGTTATGATGCCTTGGAAGGAGGAAG CTACCCAGATATGCATTCTTCTTCAGCAAGCAGCCCCGTTCCTGATCGTGCCCCGGAACCTAACTCCACC CTCGTCCCCACTCCCACTGCTGCTCAGCCAGCCAAGGTAGCAAAGCCTTTTGGCTATGGCTACCCAACGC TGCAGCCTGCTTATCAGAATGCAGCAGCACCACCCACCACAGCCCACCCCAGCGGGCCAGCGTATTCTGG ATATCCTCAGCAGTATCCAGGTGTGCACCAACTGTCCTCAGGTCTGGGAGGGCTAAGCCTGCAGAGCTCT CCACAGCCAGAAAGCCTACGGCCTGTGAACCTCACCCAGGAGAAGAACATCTTACCTGCAACTCCCATTT GGGCACCTGTACCTAACCTGAGTGCAGAGCTCAGCAAGTTAAACTGTAGCCCAGACTCGTTTCGATGTAC CTTGACCAGTATCCCACAGACACAGGCTTTACTGAATAAAGCTAAGCTTCCCTTGGGACTGCTGCTGCAC CCCTTCAGAGACCTGACGCAATTGCCAGTGATAACCTCCAACACCATTGTGAGGTGCCGGTCCTGTCGGA CGTACATCAACCCTTTTGTGTCCTTCATCGATCAGCGCAGGTGGAAATGCAACCTGTGCTACAGAGTGAA TGATGTTCCAGAAGAGTTTTTGTACAACCCCCTGACTCGGTCTTACGGAGAGCCTCATAAACGGCCGGAA GTTCAGAATTCAACTGTGGAGTTCATCGCTTCCTCAGACTACATGCTCCGGCCTCCTCAGCCTGCAGTCT ACTTGTTTGTTTTAGATGTGTCTCATAACGCAGTGGAAGCTGGGTACTTGACAGTTCTATGCCAGTCCTT ATTAGAAAACTTAGACAAGCTTCCTGGAGACTCACGAACAAGAATAGGATTCATGACTTTCGACAGCACT ATTCATTTCTACAATTTACAAGAAGGATTATCCCAGCCTCAGATGTTGATTGTATCTGATATAGACGATG TTTTCTTGCCCACACCGGATAGTCTGCTTGTGAATCTGTATGAAAGTAAAGAGCTTATAAAAGACTTACT GAACGCATTACCAAATATGTTCATCAATACCAGAGAAACGCACAGTGCTCTTGGACCTGCACTTCAGGCT GCCTTCAAACTAATGTCTCCCACGGGCGGACGAGTGTCTGTGTTTCAGACACAGTTACCTTCCTTGGGGG CAGGACTTCTGCAGTCCAGAGAAGATCCGAATCAGAGGTCGAGCACCAAGGTGGTGCACCATCTCGGCCC TGCCACCGACTTTTATAAGAAACTTGCTTTAGATTGCTCAGGACAGCAGACCGCAGTGGATTTGTTCCTG CTAAGTTCACAGTATTCTGACCTCGCTTCTCTGGCCTGCATGTCCAAGTACTCCGCAGGGTGCATATTCT ACTACCCATCGTTCCACTCCACCCACAACCCTTCGCAAGCAGAGAAGTTACAGAAAGACCTAAAACGGTA CCTCACCAGGAAGATCGGATTTGAAGCTGTTATGAGGATAAGATGTACCAAAGGACTTTCGATGCACACT TTCCACGGGAACTTCTTTGTCCGGTCCACTGACTTGCTGTCTCTTGCCAACATCAATCCTGATGCTGGGT TTGCAGTGCAGTTGTCAATTGAGGAAAGCCTGGCGGACACTTCCCTGGTGTGCTTCCAGACTGCCCTGCT GTACACATCAAGCAAAGGTGAGCGGAGAATTCGAGTTCACACACTGTGCTTGCCAGTGGTGAGTTCACTG GCTGATGTTTATGCAGGAGTGGATGTGCAAGCTGCCATCTGCCTTCTGGCCAACATGGCTGTGGACCGCT CAGTGTCATCCAGCCTGTCGGATGCCAGGGACGCCCTGGTGAATGCCGTGGTGGATCCCCTGTCTGCCTA CAGCTCTGCAGTGTCGAGTGTGCCCCGCTCCACCCTGACAGCACCGAGCTCACTGAAGCTGCTCCCTCTC TATGTTCTGGCACTTCTCAAACAGAAAGCATTTAGAACAGGTACAAGCACGCGCCTGGACGACCGTGTGT ACGCCATGTGCCAGATGAAGTCGCAGCCACTTGTGCATCTAATGAAAATGATTCATCCCAACTTGTACAG GATAGACCGGCTGACGGATGAGGGTGCCATACATGTCAACGACAGGGTAGTACCCCAGCCACCTCTTCAA AAACTGTCTGCAGAGAAACTGACCAGAGAAGGTGCTTTCCTCATGGACTGTGGCTCAGTTTTCTACATTT GGATTGGCAAAGGCTGTGACAGTAATTTCATAGAGAATGTGCTTGGGTATCCTGATTTTGGATCAATACC ACAGAAAATGACACATCTTCCAGAGCTAGACACACTTCCGTCAGAGAGAACTAGATCCTTTGTAACTTGG CTCAGAGACAGCCGACCATTAAGCCCTGTCCTTCACGTGGTAAAAGATGAGAGTCCTGCCAGAACAGACT TTTTCCAGCATTTGGTGGAAGACCGCACCGAAGCCGCACTCTCCTACTATGAGTTTCTGATTCACGTTCA GCAGCAGGTTTGTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR215085 representing NM_001106474 Red=Cloning site Green=Tags(s) MSAPAGSPHPAAGARMPPKLGGAVSGLAPPQQNGPAQSQMQVPSGYGLPHQNYMAPSGHYSQGPGKMTSL PLDNQCENYYSRPYTAPTQNVGTPSSANQPGAQLMYGRGPSAPHMGASMPGPFQGAPASASHSYPSASQP YSSLGNRYSSPATYSATASVASQGYPSTCSHYPISTVSNVVYPNVSYPSLPASEPYGQMFTSQSAPPPAR PLKESYSGPSTAVAYPSRPPPPPSQHQQQQQQQQQQQQQSHSGYSSLPWSGPGLPPAQDSLIRNQMGSLA IPNSHPAINENVQPPKPSSVVATVLPGPSSTRMPPAPSHPVGPVPSAPPPPEQMQTKGMQYGDYVNNQAS STPTPLSSASDDEEEEDEDEEAGVDSSSTTSSASPLPNSYDALEGGSYPDMHSSSASSPVPDRAPEPNST LVPTPTAAQPAKVAKPFGYGYPTLQPAYQNAAAPPTTAHPSGPAYSGYPQQYPGVHQLSSGLGGLSLQSS PQPESLRPVNLTQEKNILPATPIWAPVPNLSAELSKLNCSPDSFRCTLTSIPQTQALLNKAKLPLGLLLH PFRDLTQLPVITSNTIVRCRSCRTYINPFVSFIDQRRWKCNLCYRVNDVPEEFLYNPLTRSYGEPHKRPE VQNSTVEFIASSDYMLRPPQPAVYLFVLDVSHNAVEAGYLTVLCQSLLENLDKLPGDSRTRIGFMTFDST IHFYNLQEGLSQPQMLIVSDIDDVFLPTPDSLLVNLYESKELIKDLLNALPNMFINTRETHSALGPALQA AFKLMSPTGGRVSVFQTQLPSLGAGLLQSREDPNQRSSTKVVHHLGPATDFYKKLALDCSGQQTAVDLFL LSSQYSDLASLACMSKYSAGCIFYYPSFHSTHNPSQAEKLQKDLKRYLTRKIGFEAVMRIRCTKGLSMHT FHGNFFVRSTDLLSLANINPDAGFAVQLSIEESLADTSLVCFQTALLYTSSKGERRIRVHTLCLPVVSSL ADVYAGVDVQAAICLLANMAVDRSVSSSLSDARDALVNAVVDPLSAYSSAVSSVPRSTLTAPSSLKLLPL YVLALLKQKAFRTGTSTRLDDRVYAMCQMKSQPLVHLMKMIHPNLYRIDRLTDEGAIHVNDRVVPQPPLQ KLSAEKLTREGAFLMDCGSVFYIWIGKGCDSNFIENVLGYPDFGSIPQKMTHLPELDTLPSERTRSFVTW LRDSRPLSPVLHVVKDESPARTDFFQHLVEDRTEAALSYYEFLIHVQQQVCK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106474
ORF Size	3726 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106474.1, NP_001099944.1
RefSeq Size	5001 bp
RefSeq ORF	3729 bp
Locus ID	295461
MW	134.7 kDa

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