Cit (NM_001029911) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR215463 representing NM_001029911 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGAAGTTCAAGTATGGAGTGCGGAACCCGTCCGAGGCCAGTGCCCCCGAGCCCATTGCCAGTCGGG CCTCCAGGCTAAATCTCTTCTTCCAGGGGAAACCGCCCCTCATGACTCAACAGCAGATGTCTGCTCTTTC CCGGGAAGGGGTGTTAGATGCCCTCTTTGTTCTCTTGGAAGAGTGCAGTCAGCCTGCCCTCATGAAGATA AAGCACGTGAGCAGCTTCGTCCGGAAGTATTCTGACACGATAGCCGAGTTACGGGAGCTCCAGCCGTCGG TGAGGGACTTCGAAGTGCGAAGTCTTGTGGGCTGCGGTCACTTCGCCGAAGTGCAGGTGGTTAGAGAGAA GGCCACCGGGGATGTCTACGCCATGAAGATCATGAAGAAGGCGGCTTTGCGGGCCCAGGAGCAGGTTTCA TTTTTCGAGGAGGAGAGGAACATATTATCCCAGAGCACGAGCCCTTGGATCCCCCAATTACAGTACGCCT TTCAGGACAAAAATAACCTTTACCTGGTCATGGAGTATCAGCCTGGAGGGGATTTGCTGTCTCTTCTGAA TAGATATGAGGACCAGTTAGATGAAAACATGATTCAGTTTTACCTTGCTGAGCTGATCTTGGCTGTCCAC AGCGTGCACCAGATGGGATATGTGCATCGAGACATCAAGCCGGAGAACATCCTCATTGACCGAACGGGAC ACATCAAGCTGGTGGATTTTGGATCAGCCGCTAAGATGAATTCAAATAAGGTGGATGCCAAACTCCCTAT TGGGACCCCAGATTACATGGCTCCGGAAGTGTTGACTGTGATGAACGAGGACCGAAGGGGCACCTACGGC CTGGACTGTGACTGGTGGTCTGTCGGAGTGGTTGCCTATGAGATGCTTTACGGGAAGACCCCATTCACGG AGGGAACCTCCGCCCGGACCTTCAACAACATCATGAACTTCCAGCGGTTTTTGAAGTTCCCGGATGACCC CAAAGTTAGCAGTGAGCTCCTTGATCTGATTCAGAGTTTGCTGTGTGTCCAGAAAGAGAGACTGAAGTTC GAGGGTCTCTGTTGCCACCCTTTCTTTGCCAGAACCGACTGGAATAACATCCGGAACTCTCCTCCCCCCT TCGTTCCCACCCTCAAGTCTGACGATGACACCTCCAATTTTGATGAACCAGAGAAGAATTCGTGGGTTTC ATCCTCTCCGTGCCAGCTGAGCCCTTCGGGTTTCTCGGGCGAAGAGCTGCCCTTTGTGGGGTTTTCATAC AGCAAGGCACTGGGGTATCTTGGTAGATCTGAGTCTGTTGTGTCGGGTCTGGACTCCCCTGCCAAGATTA GCTCCATGGAAAAGAAACTTCTCATCAAAAGCAAAGAGCTGCAAGACTCTCAGGACAAGTGTCACAAGAT GGAGCAGGAAATGGCCCGGTTGCATCGCAGAGTGTCAGAGGTGGAGGCTGTGCTTAGTCAGAAGGAGGTG GAGCTGAAGGCCTCTGAGACTCAGAGATCCCTCCTGGAGCAGGACCTTGCCACCTACATCACAGAATGCA GTAGCTTAAAGCGAAGTCTGGAGCAAGCGCGGATGGAGGTGTCTCAGGAGGATGACAAAGCTCTTCAGCT TCTCCACGACATCAGAGAGCAGAGCCGGAAGCTCCAGGAGATCAAGGAGCAGGAGTACCAGGCTCAGGTG GAGGAGATGAGGCTGATGATGAATCAGCTGGAAGAGGACCTCGTGTCAGCCCGAAGACGCAGCGATCTCT ACGAGTCTGAGCTGAGGGAGTCTCGGCTCGCCGCCGAGGAATTCAAGCGGAAGGCAAACGAATGTCAGCA TAAACTGATGAAGGCTAAGGACCTAGGGAAGCCTGAAGTGGGAGAATGTTCCAGACTGGAGAAGATCAAT GCTGAGCAGCAGCTGAAGATTCAGGAGCTCCAGGAGAAGCTGGAGAAGGCTGTAAAAGCCAGCACCGAGG CCACAGAGCTCCTGCAGAACATCCGGCAGGCAAAGGAGCGAGCTGAGAGGGAGCTGGAGAAGCTGCACAA CAGGGAAGATTCTTCCGAAGGCATCAAAAAGAAACTGGTGGAAGCTGAGGAACGCCGCCACTCCCTGGAG AACAAGGTAAAGAGACTAGAGACCATGGAGCGTAGAGAGAACAGACTGAAGGATGACATCCAGACAAAGT CCGAACAGATCCAGCAGATGGCTGATAAAATTCTGGAGCTGGAGGAGAAGCATCGGGAGGCTCAGGTCTC AGCTCAACACCTAGAAGTACACCTGAAGCAGAAGGAACAGCACTATGAGGAAAAGATCAAAGTGTTGGAC AATCAGATAAAGAAGGACTTGGCCGACAAGGAGAGCCTAGAGACCATGATGCAGAGGCACGAAGAGGAGG CCCACGAGAAGGGCAAGATCCTCAGCGAGCAGAAGGCGATGATCAACGCAATGGATTCCAAGATCAGATC CCTGGAGCAGAGGATTGTGGAGCTGTCAGAAGCCAACAAGCTTGCGGCAAACAGCAGTCTCTTCACCCAG AGGAACATGAAGGCCCAGGAAGAGATGATCTCAGAACTCAGGCAGCAGAAATTTTACCTGGAGACACAGG CCGGGAAGCTGGAGGCCCAGAACCGAAAACTGGAAGAGCAACTAGAGAAGATCAGCCACCAAGATCACAG TGACAAGAATCGGCTGCTGGAGCTGGAGACAAGGTTGAGAGAGGTCAGCCTGGAGCATGAGGAGCAGAAG CTGGAGCTGAAGCGGCAGCTCACGGAGCTGCAGCTGTCCCTGCAGGAGCGTGAGTCCCAGCTGACGGCCC TGCAGGCCGCCCGGGCTGCCCTTGAGAGCCAGCTCCGCCAGGCGAAGACGGAGCTGGAGGAGACAACTGC AGAAGCGGAGGAGGAGATCCAGGCACTCACGGCACATCGAGATGAAATCCAGCGCAAATTCGATGCCCTT CGCAACAGCTGTACTGTCATCACCGACCTGGAGGAGCAGCTGAACCAGCTCACCGAGGACAACGCCGAGC TCAACAACCAAAACTTCTACCTGTCCAAACAACTCGATGAGGCTTCGGGTGCCAACGATGAGATTGTGCA GCTGCGAAGCGAGGTGGACCATCTCCGCCGTGAGATCACGGAGCGGGAGATGCAGCTCACCAGCCAGAAG CAAACAATGGAGGCTCTGAAGACGACCTGCACCATGCTGGAGGAGCAGGTCATGGACCTGGAGGCCCTGA ACGACGAGCTGCTGGAGAAGGAGCGCCAGTGGGAGGCCTGGAGGAGCGTCCTCGGGGACGAGAAGTCTCA GTTTGAGTGTCGAGTTCGAGAGCTACAGAGGATGCTGGACACCGAGAAGCAGAGCAGGGCTAGAGCTGAT CAGCGGATCACGGAGTCTCGCCAGGTGGTGGAGCTGGCAGTGAAGGAGCACAAAGCCGAGATTCTCGCGC TGCAGCAGGCTCTCAAAGAGCAGAAGCTGAAAGCTGAGAGCCTGTCGGACAAGCTCAACGACCTGGAGAA GAAGCACGCCATGCTTGAGATGAATGCCCGGAGCTTACAGCAGAAACTGGAGACAGAGCGGGAGCTCAAA CAGAGGCTTCTGGAGGAGCAAGCCAAGTTGCAGCAGCAGATGGACCTGCAGAAGAACCACATCTTCAGAC TGACACAAGGGCTGCAGGAGGCGCTGGACCGGGCGGATCTGCTGAAGACCGAAAGGAGCGACCTGGAATA TCAGCTGGAAAACATTCAGGTTCTCTACTCACACGAGAAGGTGAAAATGGAAGGCACTATTTCTCAACAA ACCAAACTCATTGATTTCCTGCAAGCCAAAATGGACCAGCCCGCTAAAAAGAAAAAGGTTCCTCTGCAGT ACAATGAGCTGAAGCTCGCCCTGGAAAAGGAGAAAGCGCGCTGTGCGGAGCTGGAGGAAGCCCTTCAGAA GACCCGCATCGAACTCCGGTCCGCACGGGAGGAAGCTGCTCACCGCAAAGCCACGGACCACCCGCACCCA TCTACGCCAGCCACTGCGAGGCAGCAGATCGCCATGTCCGCCATCGTGCGGTCGCCTGAGCACCAGCCCA GTGCCATGAGCCTGCTCGCCCCACCATCCAGCCGCAGAAAGGAGGCGTCAACTCCAGAGGAGTTCAGCCG GCGTCTTAAAGAACGTATGCACCACAACATTCCTCACCGGTTTAACGTGGGCCTGAATATGCGAGCCACC AAGTGCGCCGTGTGTCTGGATACTGTGCACTTTGGACGCCAGGCATCCAAATGTCTCGAATGTCAGGTGA TGTGCCATCCCAAGTGTTCCACCTGCTTGCCCGCCACCTGTGGCCTACCTGCCGAATATGCCACACACTT CACCGAGGCCTTCTGCCGAGACAAAGTGAGCTCCCCGGGGCTCCAGAGCAAGGAGCCCAGCAGCAGCTTG CACCTGGAAGGGTGGATGAAAGTGCCCAGGAATAACAAACGAGGACAGCAAGGCTGGGACAGAAAGTACA TTGTCTTAGAGGGTTCAAAAGTCCTCATCTATGACAACGAAGCCAGAGAAGCTGGACAGAGGCCGGTGGA AGAATTTGAGCTGTGCCTTCCCGATGGGGATGTATCTATTCATGGTGCCGTTGGTGCTTCTGAACTTGCA AATACAGCCAAAGCAGATGTCCCATACATACTGAAGATGGAGTCCCATCCGCATACCACCTGCTGGCCTG GGAGAACCCTCTACCTGCTGGCACCCAGCTTTCCTGACAAGCAGCGCTGGGTCACAGCCCTAGAGTCTGT CGTCGCAGGTGGGAGAGTTTCTAGGGAAAAGGCAGAGGCCGATGCTAAATTACTTGGAAACTCTCTGCTG AAGCTGGAAGGTGACGACCGGCTTGACATGAACTGTACCCTGCCCTTCAGCGACCAGGTGGTGCTGGTGG GCACCGAGGAGGGCCTCTATGCGCTGAACGTCTTGAAAAACTCCTTAACCCACATCCCAGGAATCGGAGC AGTCTTCCAGATTTACATCATCAAGGACCTGGAGAAGCTGCTCATGATAGCAGGGGAAGAGCGGGCTCTG TGTTTGGTGGACGTGAAGAAGGTGAAGCAGTCGTTGGCACAGTCACACCTTCCTGCCCAGCCTGACGTCT CCCCCAACATATTTGAAGCCGTCAAAGGCTGCCACCTGTTTGCTGCTGGCAAGATCGAGAACAGCCTCTG CATCTGTGCCGCCATGCCGAGCAAAGTCGTCATCCTGCGCTACAACGACAACCTCAGCAAGTTCTGCATC CGCAAGGAGATTGAGACCTCTGAGCCCTGCAGCTGTATCCACTTCACCAATTACAGTATCCTCATCGGAA CCAACAAGTTCTATGAGATCGACATGAAACAGTACACGCTTGAGGAGTTCCTGGACAAGAATGACCATTC CTTGGCACCTGCTGTGTTCGCATCCTCGACCAACAGCTTCCCCGTCTCCATCGTGCAGGCAAACAGCACC GGGCAGCGGGAAGAGTACCTGCTGTGTTTCCACGAATTTGGGGTGTTCGTGGATTCTTATGGAAGACGTA GCCGCACAGACGATCTCAAGTGGAGTCGCTTACCTCTGGCCTTTGCCTACAGAGAGCCTTATCTGTTTGT GACTCACTTTAACTCCCTGGAAGTAATTGAGATCCAGGCACGTTCCTCACTGGGGACCCCTGCCCGAGCC TATCTGGAAATCCCAAACCCTCGCTACCTGGGCCCCGCAATTTCCTCCGGAGCAATTTACCTGGCCTCCT CATACCAGGACAAGTTAAGGGTCATATGCTGCAAAGGAAACCTCGTGAAGGAGTCTGGCACTGAGCAGCA CCGCGTGCCCTCCACATCCCGCAGCAGCCCTAACAAGCGAGGCCCACCAACATACAACGAGCACATCACC AAACGTGTGGCCTCCAGCCCAGCACCACCAGAAGGCCCCAGCCATCCCCGAGAGCCAAGCACACCCCACC GCTACCGAGACAGAGAGGGCCGGACAGAGCTGCGCAGGGACAAGTCTCCAGGCCGTCCTCTGGAGCGGGA GAAGTCGCCAGGCCGGATGCTCAGCACGAGGAGAGAGCGGTCCCCGGGGAGACTGTTTGAAGACAGCAGC AGGGGCCGGCTGCCTGCGGGAGCCGTGAGGACCCCACTGTCCCAGGTTAACAAGGTCTGGGACCAGTCTT CAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR215463 representing NM_001029911 Red=Cloning site Green=Tags(s) MLKFKYGVRNPSEASAPEPIASRASRLNLFFQGKPPLMTQQQMSALSREGVLDALFVLLEECSQPALMKI KHVSSFVRKYSDTIAELRELQPSVRDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKAALRAQEQVS FFEEERNILSQSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDENMIQFYLAELILAVH SVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYG LDCDWWSVGVVAYEMLYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLIQSLLCVQKERLKF EGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSPCQLSPSGFSGEELPFVGFSY SKALGYLGRSESVVSGLDSPAKISSMEKKLLIKSKELQDSQDKCHKMEQEMARLHRRVSEVEAVLSQKEV ELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQV EEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDLGKPEVGECSRLEKIN AEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLE NKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLD NQIKKDLADKESLETMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQ RNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKNRLLELETRLREVSLEHEEQK LELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDAL RNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQK QTMEALKTTCTMLEEQVMDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARAD QRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELK QRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQ TKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHP STPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKEASTPEEFSRRLKERMHHNIPHRFNVGLNMRAT KCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKVSSPGLQSKEPSSSL HLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELA NTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLL KLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERAL CLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKFCI RKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLEEFLDKNDHSLAPAVFASSTNSFPVSIVQANST GQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGTPARA YLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHIT KRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSS RGRLPAGAVRTPLSQVNKVWDQSSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001029911
ORF Size	6165 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001029911.1, NP_001025082.1
RefSeq Size	6291 bp
RefSeq ORF	6168 bp
Locus ID	83620
UniProt ID	E9PSL7
MW	235.3 kDa
Gene Summary	a serine/threonine kinase; involved in regulating G2/M transition [RGD, Feb 2006]

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