Ascl1 (NM_022384) Rat Tagged ORF Clone
CAT#: RR215503
- TrueORF®
Ascl1 (Myc-DDK-tagged ORF) - Rat achaete-scute complex homolog 1 (Drosophila) (Ascl1), (10 ug)
"NM_022384" in other vectors (5)
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CNY 2,400.00
CNY 3,990.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Mash1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR215503 representing NM_022384
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGCTCTGGCAAGATGGAGAGCGGAGCCGGCCAGCAGCCGCAGCCTCCGCAGCCCTTCCTGCCGC CTGCAGCCTGCTTCTTTGCGACCGCGGCGGCCGCGGCGGCGGCGGCGGCGGCAGCGGCAGCTCAGAGCGC GCAGCAGCAACAGCAGCAGCAGGCGCCGCAGCAGCAGGCGCCGCAGCTGAGCCCGGTGGCGGACGGCCAG CCCTCAGGGGGCGGTCACAAGTCAGCGGCCAAGCAGGTCAAGCGCCAGCGCTCGTCCTCTCCCGAACTGA TGCGCTGCAAACGCCGGCTCAACTTCAGTGGCTTCGGCTACAGCCTTCCACAGCAGCAGCCGGCAGCCGT GGCGCGCCGCAACGAGCGCGAGCGCAACCGGGTCAAGTTGGTTAACCTGGGCTTTGCCACCCTCCGGGAG CATGTCCCCAACGGCGCTGCCAACAAGAAGATGAGCAAGGTGGAGACGCTGCGTTCGGCGGTCGAATACA TCCGCGCGCTGCAGCAGCTGCTGGACGAGCACGATGCGGTGAGCGCTGCCTTCCAGGCGGGCGTCCTGTC GCCCACCATCTCCCCCAACTACTCCAACGACTTGAACTCTATGGCGGGTTCTCCGGTTTCGTCCTACTCC TCCGACGAGGGATCCTACGACCCCCTTAGTCCAGAGGAACAAGAGCTGCTGGACTTTACCAACTGGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR215503 representing NM_022384
Red=Cloning site Green=Tags(s) MESSGKMESGAGQQPQPPQPFLPPAACFFATAAAAAAAAAAAAAQSAQQQQQQQAPQQQAPQLSPVADGQ PSGGGHKSAAKQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPAAVARRNERERNRVKLVNLGFATLRE HVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAGSPVSSYS SDEGSYDPLSPEEQELLDFTNWF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_022384 |
ORF Size | 699 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 1656 bp |
RefSeq ORF | 702 bp |
Locus ID | 64186 |
UniProt ID | P19359 |
MW | 25 kDa |
Gene Summary | plays a role in neurogenesis of the autonomic nervous system [RGD, Feb 2006] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Significance of achaete-scute complex homologue 1 (ASCL1) in pulmonary neuroendocrine carcinomas; RNA sequence analyses using small cell lung cancer cells and Ascl1-induced pulmonary neuroendocrine carcinoma cells.
,null,
Histochemistry and cell biology
,PubMed ID 32170367
[Ascl1]
|
Combinatorial programming of human neuronal progenitors using magnetically-guided stoichiometric mRNA delivery
,null,
eLife
,PubMed ID 29714688
[Ascl1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN215503 | Ascl1 (untagged ORF) - Rat achaete-scute complex homolog 1 (Drosophila) (Ascl1), (10 ug) |
CNY 3,990.00 |
|
RR215503L1 | Lenti ORF clone of Ascl1 (Myc-DDK-tagged ORF) - Rat achaete-scute complex homolog 1 (Drosophila) (Ascl1), (10 ug) |
CNY 6,080.00 |
|
RR215503L2 | Lenti ORF clone of Ascl1 (mGFP-tagged ORF) - Rat achaete-scute complex homolog 1 (Drosophila) (Ascl1), (10 ug) |
CNY 4,800.00 |
|
RR215503L3 | Lenti ORF clone of Ascl1 (Myc-DDK-tagged ORF) - Rat achaete-scute complex homolog 1 (Drosophila) (Ascl1), (10 ug) |
CNY 6,080.00 |
|
RR215503L4 | Lenti ORF clone of Ascl1 (mGFP-tagged ORF) - Rat achaete-scute complex homolog 1 (Drosophila) (Ascl1), (10 ug) |
CNY 6,650.00 |