Pdx1 (NM_022852) Rat Tagged ORF Clone
CAT#: RR215611
- TrueORF®
Pdx1 (Myc-DDK-tagged ORF) - Rat pancreatic and duodenal homeobox 1 (Pdx1), (10 ug)
"NM_022852" in other vectors (3)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Idx1; Ipf1; Stf1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR215611 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATAGTGAGGAGCAGTACTACGCGGCCACACAGCTCTACAAGGACCCGTGCGCATTCCAGAGGGGTC CGGTGCCAGAGTTCAGTGCTAATCCCCCTGCGTGCCTGTACATGGGCCGCCAGCCCCCACCTCCGCCGAC ACCCCAGTTTGCAGGCTCGCTGGGAACGCTGGAACAGGGAAGTCCCCCGGACATCTCCCCATACGAAGTG CCCCCGCTCGCCGATGACCCGGCTGGCGCGCACCTCCACCACCACCTCCCAGCTCAGCTCGGGCTCGCCC ATCCACCTCCCGGACCTTTCCCGAATGGAACCGAGACTGGGGGCCTGGAAGAGCCCAGCCGCGTTCATCT CCCTTTCCCGTGGATGAAATCCACCAAAGCTCACGCGTGGAAAAGCCAGTGGGCAGGAGGTGCATACGCA GCAGAACCGGAGGAGAATAAGAGGACCCGTACAGCCTACACTCGGGCCCAGCTGCTGGAGCTGGAGAAGG AATTCTTATTTAACAAATACATCTCCCGGCCTCGCCGGGTGGAGCTGGCAGTGATGCTCAACTTGACTGA GAGACACATCAAAATCTGGTTCCAAAACCGTCGCATGAAGTGGAAGAAAGAGGAAGATAAGAAACGTAGT AGCGGGACAACGAGCGGGGGCGGTGGGGGCGAAGAGCCGGAGCAGGATTGTGCCGTAACCTCGGGCGAGG AGCTGCTGGCATTGCCAAAGCCACCACCTCCCGGAGGTGTTGTGCCCTCAGGCGTCCCTGCTGCTGCCCG GGAGGGCCGACTGCCTTCCGGCCTTAGTGCGTCCCCACAGCCCTCCAGCATCGCGCCACTGCGACCGCAG GAACCCCGG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR215611 protein sequence
Red=Cloning site Green=Tags(s) MNSEEQYYAATQLYKDPCAFQRGPVPEFSANPPACLYMGRQPPPPPTPQFAGSLGTLEQGSPPDISPYEV PPLADDPAGAHLHHHLPAQLGLAHPPPGPFPNGTETGGLEEPSRVHLPFPWMKSTKAHAWKSQWAGGAYA AEPEENKRTRTAYTRAQLLELEKEFLFNKYISRPRRVELAVMLNLTERHIKIWFQNRRMKWKKEEDKKRS SGTTSGGGGGEEPEQDCAVTSGEELLALPKPPPPGGVVPSGVPAAAREGRLPSGLSASPQPSSIAPLRPQ EPR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_022852 |
ORF Size | 849 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022852.3, NP_074043.3 |
RefSeq Size | 1403 bp |
RefSeq ORF | 852 bp |
Locus ID | 29535 |
UniProt ID | P52947 |
MW | 30.9 kDa |
Gene Summary | Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN215611 | Pdx1 (untagged ORF) - Rat pancreatic and duodenal homeobox 1 (Pdx1), (10 ug) |
CNY 2,400.00 |
|
RR215611L3 | Lenti ORF clone of Pdx1 (Myc-DDK-tagged ORF) - Rat pancreatic and duodenal homeobox 1 (Pdx1), (10 ug) |
CNY 6,080.00 |
|
RR215611L4 | Lenti ORF clone of Pdx1 (mGFP-tagged ORF) - Rat pancreatic and duodenal homeobox 1 (Pdx1), (10 ug) |
CNY 6,650.00 |