Vps25 (NM_001173451) Rat Tagged ORF Clone
CAT#: RR215960
- TrueORF®
Vps25 (myc-DDK-tagged) - Rat vacuolar protein sorting 25 homolog (S. cerevisiae) (Vps25)
"NM_001173451" in other vectors (1)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR215960 representing NM_001173451
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATGAGTTTCGAGTGGCCGTGGCAGTACCGCTTCCCGCCCTTCTTTACGTTACAGCCGAACGTGG ACACCCGGCAGAAGCAGCTGGCCGCCTGGTGCTCTCTGGTTCTGTCCTTCTGCCGCCTGCACAAACAGTC CAGCATGACGGTGATGGAAGCCCAGGAGAGCCCGCTCTTCAACAACGTCAAGCTACAGCGGAAACTTCCT GTGGAGTCGATTCAGATTGTATTAGAAGAACTGAGGAAGAAAGGGAACCTCGAGTGGTTGGATAAGAATA AGTCTAGCTTCCTGATCATGTGGCGGAGGCCAGAAGAATGGGGGAAACTCATTTATCAATGGGTTTCCAG GAGTGGCCAGAATAACTCTGTGTTCACCCTGTATGAACTGACCAGTGGGGAAGACACAGAGGAGGAAGAG TTCCATGGGCTGGATGAGGCGACCCTGCTGCGTGCTCTGCAGGCCCTACAGCAGGAGCACAAGGCTGAGA TTATCACTGTCAGCGATGGCCGAGGAGTCAAATTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR215960 representing NM_001173451
Red=Cloning site Green=Tags(s) MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLP VESIQIVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTSGEDTEEEE FHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001173451 |
ORF Size | 528 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001173451.1, NP_001166922.1 |
RefSeq Size | 1090 bp |
RefSeq ORF | 531 bp |
Locus ID | 681059 |
UniProt ID | P0C0A1 |
MW | 20.8 kDa |
Gene Summary | Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex (By similarity). The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN215960 | Vps25 (untagged) - Rat vacuolar protein sorting 25 homolog (S. cerevisiae) (Vps25) |
CNY 2,000.00 |