Il20rb (NM_001173438) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	IL20R2; RGD1566151
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216305 representing NM_001173438 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTTTCCAAGGAGTCTGGACAAGTCCGATCATGTGGTTTTTCTACAGCATGACCTCAGGTTTTCTCA CTGATGGAGCGACCATTCTGCCTGCCCCACAAAACTTCTCTGTACGGTCAACCAACATGAAGCATCTGTT GATGTGGAACCCAGTGACCCAGCCGGGGGAGACGGTATTCTATTTTGTGCAGTACCAGGGGGAGTACGAG AGCCTGTACATGAGTCACATCTGGATCCCCAGCAGCCAGTGCTCACCTACCAAAGGTCTGGAGTGCGATG TCACTGATGACGTCACTGCCACAGTGCCATATAACTTCAGGGTCATGGCCATGCTGGGTTCGCAATCTTC AGCCTGGAGCAACCTGGAGCACCCCTTTAATCGGAATGCAACTGTTCTCACCCCACCCAGGATGGAGGTC ACTGAACATGGGCTGCATCTGGTTGTTGAGCTGGAAGACCTGGGGCCCCAGTTTGAATTCCTTGTGGTCT ACTGGAGGATGGAGCCCGGTGCCGTGGAACGTGTCAAGGTGGTGAGGAGTGGGGACATTCCGGTGCACCT AGAAACCATGGATCCAGGGGTCATGTACTGCGTGAAGGCGCAGGCGCTGGTGAAAACCATCGGGAGGCAC AGTGCCTTCAGCCAGCCTACGTGTGTGGAGATGCAAGGAGAGTCTCTTCCGCTGGCACTGGCTCTGTTTG CATTTGTTGGCTTCGTGCTGATTCTTGTGGTTGCACTGCTCTCCGTCTGGAAGATGGGCCAGCTGCTCCG CTATTCTTGCTGCCCCGCTGTTGTCCTTCCTGACACCTTGAAAGTAACCAGCTCATCCCAGAAGCTGATC CGCTGCAGAAAGGAGGAGGTGGATGCCTGTGCCGTGACCGTGCTGTCCTCGGAGCATCTCTTTGGGGTCT GGATCTCGCAGACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216305 representing NM_001173438 Red=Cloning site Green=Tags(s) MVFQGVWTSPIMWFFYSMTSGFLTDGATILPAPQNFSVRSTNMKHLLMWNPVTQPGETVFYFVQYQGEYE SLYMSHIWIPSSQCSPTKGLECDVTDDVTATVPYNFRVMAMLGSQSSAWSNLEHPFNRNATVLTPPRMEV TEHGLHLVVELEDLGPQFEFLVVYWRMEPGAVERVKVVRSGDIPVHLETMDPGVMYCVKAQALVKTIGRH SAFSQPTCVEMQGESLPLALALFAFVGFVLILVVALLSVWKMGQLLRYSCCPAVVLPDTLKVTSSSQKLI RCRKEEVDACAVTVLSSEHLFGVWISQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001173438
ORF Size	924 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001173438.1, NP_001166909.1
RefSeq Size	1107 bp
RefSeq ORF	927 bp
Locus ID	501043
MW	34.5 kDa

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