Bfsp2 (NM_001277434) Rat Tagged ORF Clone
CAT#: RR216614
- TrueORF®
Bfsp2 (myc-DDK-tagged) - Rat beaded filament structural protein 2, phakinin (Bfsp2)
"NM_001277434" in other vectors (1)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR216614 representing NM_001277434
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAGAGGAGAGTAGCAATGGACTTGCCCTCAGGATCCAACGCCAGCATGCCCCTGCAAAGGCACA GGGTGTCATCCCTTAGGGGAACACGATCTCCTTCCTCCCTGGATAGCCCCCCAGCATCCAGGACCAGTGC TGTAGGTAGCCTCGTCCGTGCCCCTGGGGTCTATGTAGGAGTAGCACCCAGTGGTGGCATAGGTGGCCTC GGTGCCCGAGTGACCCGCCGGGCCCTGGGCATCAGCAGTGTCTTCCTGCAGGGCCTGCGGAGCTCAGGCC TTGCCACTGCACCTGCTCCAGGCCCAGAAAGGAATCACGCCACTGCTGAGGACCTGGGGGGCTGCCTAGT GGAGTATATGACCAAGGTGCACGCTCTGGAACAAGTCAGCCAGGAGCTGGAGACACAACTACGGGCTCAC TTGGAGAGCAAGGCCAAGCGCTCCGGAGGCTGGGATGCCCTCCGTGCCTCCTGGGCCAGCAGCTACCAGC AGGTGGGAGAGGCCGTCTTGGAAAATGCCCGACTCATGCTGCAGATGGAGACCATCCAGGCCGGTGCGGA TGACTTTAAAGAGAGGTATGAGAACGAGCAGCCATTCAGGAAGGCGGCGGAAGAGGAAGTCAGCTCCCTG TACAAAGTCATCGATGAAGCTAATTTGACGAAGACGGATCTGGAGCATCAAATCGAAAGCCTGAAAGAAG AACTGGGCTCTCTGTCAAGAAGCTATGAAGAGGATGTGAAGGTTCTGTACAAACAGCTGGCGGGGTCTGA GCTGGAGCAAACAGATGTTCCCATGGGCACTGGTCTGGATGATGTCCTTGAGACGATCCGAGTTCAGTGG GAGAGAGATGTGGAAAAGAACCGAGCAGAAGCAGGAGCTGTGCTCCAAGCTAAGCAGCAGACAGAGGTAG TCCATGTATCCCAGACCCAAGAAGAAAAGCTGGCCGCTGCCCTCAGTGTAGAGTTACACGACACTTCACG CCAAGTCCAGAGTCTCCAGGCTGAGACAGAATCTTTACGGGCTCTGAAACGAGGCCTGGAAAACACCTTG CACGATGCCAAGCACTGGCATGATATGGAACTGCAGAACCTGGGTGCCGTGGTGGGCAGGCTGGAGGCAG AGCTGGCAGAGATCCACTCAGAGACAGAGCAGCAACAGCAGGAGCGGGCACACCTGCTGGCGTGCAAGGG CCAGCTACAGAAGGATGTGGCATCCTACCACGCCCTGCTGGACAGAGAGGAGAGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR216614 representing NM_001277434
Red=Cloning site Green=Tags(s) MSERRVAMDLPSGSNASMPLQRHRVSSLRGTRSPSSLDSPPASRTSAVGSLVRAPGVYVGVAPSGGIGGL GARVTRRALGISSVFLQGLRSSGLATAPAPGPERNHATAEDLGGCLVEYMTKVHALEQVSQELETQLRAH LESKAKRSGGWDALRASWASSYQQVGEAVLENARLMLQMETIQAGADDFKERYENEQPFRKAAEEEVSSL YKVIDEANLTKTDLEHQIESLKEELGSLSRSYEEDVKVLYKQLAGSELEQTDVPMGTGLDDVLETIRVQW ERDVEKNRAEAGAVLQAKQQTEVVHVSQTQEEKLAAALSVELHDTSRQVQSLQAETESLRALKRGLENTL HDAKHWHDMELQNLGAVVGRLEAELAEIHSETEQQQQERAHLLACKGQLQKDVASYHALLDREESN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001277434 |
ORF Size | 1248 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001277434.1, NP_001264363.1 |
RefSeq Size | 1549 bp |
RefSeq ORF | 1251 bp |
Locus ID | 501046 |
UniProt ID | D3ZER2 |
MW | 45.7 kDa |
Gene Summary | Required for the correct formation and organization of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2, AND CRYAA.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN216614 | Bfsp2 (untagged) - Rat beaded filament structural protein 2, phakinin (Bfsp2) |
CNY 3,990.00 |