Igf2bp2 (NM_001270598) Rat Tagged ORF Clone

CAT#: RR216970

  • TrueORF®

Igf2bp2 (myc-DDK-tagged) - Rat insulin-like growth factor 2 mRNA binding protein 2 (Igf2bp2), transcript variant 1



  "NM_001270598" in other vectors (1)

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CNY 5,700.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms RGD1305614
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR216970 representing NM_001270598
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGAACAAGCTGTACATCGGGAACCTGAGCCCCGCCGTCACTGCGGACGACCTCCGGCAGCTCTTCG
GGGACAGGAAGCTGCCCCTGGCGGGACAGGTCCTACTCAAGTCCGGCTACGCCTTCGTGGACTACCCCGA
CCAGAACTGGGCCATCCGCGCCATCGAGACCCTCTCGGGTAAAGTGGAGTTGCATGGGAAAATCATGGAA
GTTGATTACTCAGTCTCTAAAAAGCTAAGGAGCAGAAAAATCCAGATTCGAAACATCCCGCCTCACCTGC
AGTGGGAGGTGTTGGATGGCCTGTTGGCTGAGTATGGGACGGTAGAGAACGTGGAGCAAGTCAACACGGA
CACAGAAACAGCCGTTGTCAACGTCACCTATATGACAAGGGAAGAGGCAAAGGTAGCTATTGAGAAGTTA
AGTGGGCATCAGTTCGAGGACTACTCCTTCAAGATTTCCTACATCCCCGACGAAGAATTGAGCTCCCCTT
CACCCCCTCATCGAGCCCGGGAACAAGGCCATGGCCCCGGGAGCTCTTCTCAGGCCAGACAGATTGACTT
CCCGCTGAGGATCCTGGTCCCCACCCAGTTTGTTGGTGCCATCATCGGAAAGGAGGGCTTGACCATAAAG
AACATCACTAAACAGACCCAGTCCCGGGTAGACATCCACAGGAAGGAGAACTCTGGGGCTGCCGAGAAGC
CTGTCACAATCCACGCCACCCCAGAAGGGACATCTGAAGCCTGCCGCATGATTCTTGAGATTATGCAGAA
AGAGGCTGATGAGACCAAACTGGCAGAAGAGGTTCCTTTGAAAATCTTGGCCCACAACGGCTTCGTCGGA
AGACTGATTGGCAAAGAAGGCAGAAACCTGAAGAAAATTGAACACGAGACAGGGACCAAGATAACTATCT
CATCCTTGCAGGATTTGAGCATATATAACCCCGAGAGAACCATCACGGTGAAAGGTACCATTGAAGCCTG
TGCCAGTGCTGAGATGGAGATTATGAAGAAGCTACGCGAGGCCTTTGAAAACGACATGCTGGCCGTTAAT
CAACAAGCCAATCTGATCCCAGGGCTGAACCTCAGTGCACTCGGCATCTTTTCGACAGGACTGTCTGTGC
TTCCTCCACCAGCAGGGCCCCGTGGAGCTCCCCCGAGTCCCCCCTATCACCCATTTGCTACCCACTCCGG
ATACTTCTCCAGTCTGTACCCTCATCACCATTTCGGCCCATTCCCGCATCACCACTCGTACCCAGAGCAA
GAGACGGTGAGTCTCTTCATCCCAACGCAGGCTGTGGGTGCTATCATCGGGAAAAAGGGGGCACACATCA
AACAGCTGGCTCGATTTGCCGGTGCCTCCATCAAGATCGCTCCAGCGGAAGGTCCAGATGTCAGTGAGAG
GATGGTCATCATCACCGGGCCTCCTGAAGCCCAGTTCAAGGCTCAGGGACGGATCTTTGGGAAACTGAAA
GAAGAAAACTTCTTTAATCCCAAAGAAGAGGTGAAGCTGGAGGCCCACATCCGAGTTCCCTCCTCGACAG
CTGGCCGGGTGATTGGCAAAGGCGGTAAAACCGTAAATGAGCTGCAGAACTTGACAAGTGCAGAAGTTAT
AGTGCCTCGTGACCAAACGCCAGATGAGAACGAGGAAGTGATCGTCAGAATTATCGGGCATTTTTTTGCT
AGCCAGACTGCACAACGCAAGATCAGGGAAATTGTACAGCAGGTGAAGCAGCAGGCGCAGAAATACCCTC
AGGGAGTCGCCCCACAGCGCAGCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR216970 representing NM_001270598
Red=Cloning site Green=Tags(s)

MMNKLYIGNLSPAVTADDLRQLFGDRKLPLAGQVLLKSGYAFVDYPDQNWAIRAIETLSGKVELHGKIME
VDYSVSKKLRSRKIQIRNIPPHLQWEVLDGLLAEYGTVENVEQVNTDTETAVVNVTYMTREEAKVAIEKL
SGHQFEDYSFKISYIPDEELSSPSPPHRAREQGHGPGSSSQARQIDFPLRILVPTQFVGAIIGKEGLTIK
NITKQTQSRVDIHRKENSGAAEKPVTIHATPEGTSEACRMILEIMQKEADETKLAEEVPLKILAHNGFVG
RLIGKEGRNLKKIEHETGTKITISSLQDLSIYNPERTITVKGTIEACASAEMEIMKKLREAFENDMLAVN
QQANLIPGLNLSALGIFSTGLSVLPPPAGPRGAPPSPPYHPFATHSGYFSSLYPHHHFGPFPHHHSYPEQ
ETVSLFIPTQAVGAIIGKKGAHIKQLARFAGASIKIAPAEGPDVSERMVIITGPPEAQFKAQGRIFGKLK
EENFFNPKEEVKLEAHIRVPSSTAGRVIGKGGKTVNELQNLTSAEVIVPRDQTPDENEEVIVRIIGHFFA
SQTAQRKIREIVQQVKQQAQKYPQGVAPQRSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001270598
ORF Size 1776 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001270598.1, NP_001257527.1
RefSeq Size 3629 bp
RefSeq ORF 1779 bp
Locus ID 303824
MW 65.4 kDa
Gene Summary The protein encoded by this gene is a member of a family of mRNA binding proteins that have been shown to bind to several different mRNAs in mammalian cells, including insulin-like growth factor 2 mRNA. Generation of two different isoforms by alternative translation initiation at an internal start codon, has been described for this gene (PMID:22427968). [provided by RefSeq, Jul 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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