Chat (NM_001170593) Rat Tagged ORF Clone
CAT#: RR217035
- TrueORF®
Chat (myc-DDK-tagged) - Rat choline O-acetyltransferase (Chat)
"NM_001170593" in other vectors (1)
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CNY 6,270.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR217035 representing NM_001170593
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCATCCTGGAAAAGGCTCCCCAAAAGATGCCTGTAAAGGCTTCTAGCTGGGAGGAGCTGGACTTAC CTAAATTACCAGTGCCCCCGCTGCAGCAAACCCTGGCCACCTACCTACAGTGCATGCAGCATCTGGTACC TGAAGAGCAGTTCAGGAAGAGCCAGGCCATTGTGAAGCGGTTTGGGGCCCCTGGTGGCCTGGGTGAGACC CTGCAGGAAAAGCTCTTGGAGAGACAGGAGAAGACAGCCAATTGGGTCTCTGAATACTGGCTGAATGACA TGTACCTGAACAACCGCCTGGCCCTGCCAGTCAACTCCAGCCCTGCTGTGATCTTTGCTCGGCAGCACTT CCAAGACACCAATGACCAGCTAAGATTTGCAGCCTGCCTCATCTCTGGTGTGCTTAGCTACAAAACTCTG CTGGACAGCCACTCCCTTCCCACTGACTGGGCCAAGGGGCAGCTCTCAGGGCAGCCCCTCTGTATGAAGC AATATTACAGACTCTTCTCCTCTTACCGGCTTCCTGGCCATACCCAGGACACACTGGTGGCCCAGAAGAG CAGTATCATGCCCGAGCCTGAGCATGTCATCGTGGCCTGCTGCAACCAGTTCTTTGTCTTGGATGTTGTC ATTAATTTCCGCCGTCTCAGTGAGGGTGATCTGTTCACTCAGTTGAGAAAGATAGTCAAAATGGCGTCCA ACGAGGATGAACGCCTGCCTCCAATCGGCCTGCTGACGTCAGACGGGAGGAGCGAGTGGGCCAAGGCCAG GACGGTCCTCTTAAAAGACTCCACCAACCGGGACTCCCTGGACATGATCGAGCGCTGCATCTGCCTGGTA TGCCTGGATGGTCCAGGCACAGGAGAGCTCAGTGACACCCACAGGGCCCTCCAACTCCTTCACGGTGGAG GCTGCAGCCTGAACGGAGCCAATCGCTGGTATGACAAGTCCCTGCAGTTCGTGGTGGGCCGAGATGGCAC CTGCGGTGTGGTGTGTGAGCATTCTCCATTTGATGGCATCGTCCTGGTGCAGTGCACAGAGCACCTGCTG AAACATATGATGACAAGCAACAAGAAGCTCGTCAGGGCTGACTCAGTGAGTGAGCTCCCTGCCCCCAGAA GGCTGAGGTGGAAATGTTCCCCAGAAACCCAAGGCCATCTCGCCTCCTCAGCAGAGAAACTTCAAAGAAT AGTAAAGAATCTGGATTTCATTGTTTACAAGTTTGACAACTATGGAAAAACATTTATCAAGAAGCAGAAA TACAGCCCCGACGGCTTCATCCAGGTGGCCCTCCAGCTGGCTTACTACAGGCTTTACCAGAGACTGGTGC CCACCTATGAGAGCGCATCCATCCGCCGCTTCCAGGAAGGTCGGGTGGACAACATCAGATCAGCCACTCC AGAGGCTCTGGCTTTTGTGCAAGCCATGACTGACCACAAGGCTGCCATGCCGGCTTCTGAGAAACTGCAG CTGCTGCAGACAGCCATGCAGGCCCAGACTGAGTACACAGTCATGGCCATAACCGGCATGGCCATTGACA ACCATCTTCTGGCACTGAGGGAGCTGGCCCGAGACCTGTGCAAAGAGCCACCTGAGATGTTCATGGATGA AACATACCTGATGAGCAACCGGTTTGTCCTCTCCACCAGCCAGGTGCCCACAACCATGGAGATGTTCTGC TGTTATGGACCCGTGGTCCCCAATGGCTATGGAGCCTGCTACAACCCCCAGCCCGAGGCCATCACCTTCT GCATCTCCAGTTTTCACAGCTGCAAAGAGACCTCGTCTGTGGAGTTTGCGGAAGCTGTGGGAGCGAGCCT TGTTGACATGAGAGACCTCTGCAGTTCAAGGCAGCCTGCTGACAGCAAGCCACCAGCACCCAAGGAAAAA GCTAGGGGCCCAACCAAGCCAAGCAATCTTGACTCCAGCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR217035 representing NM_001170593
Red=Cloning site Green=Tags(s) MPILEKAPQKMPVKASSWEELDLPKLPVPPLQQTLATYLQCMQHLVPEEQFRKSQAIVKRFGAPGGLGET LQEKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFQDTNDQLRFAACLISGVLSYKTL LDSHSLPTDWAKGQLSGQPLCMKQYYRLFSSYRLPGHTQDTLVAQKSSIMPEPEHVIVACCNQFFVLDVV INFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAKARTVLLKDSTNRDSLDMIERCICLV CLDGPGTGELSDTHRALQLLHGGGCSLNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLL KHMMTSNKKLVRADSVSELPAPRRLRWKCSPETQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQK YSPDGFIQVALQLAYYRLYQRLVPTYESASIRRFQEGRVDNIRSATPEALAFVQAMTDHKAAMPASEKLQ LLQTAMQAQTEYTVMAITGMAIDNHLLALRELARDLCKEPPEMFMDETYLMSNRFVLSTSQVPTTMEMFC CYGPVVPNGYGACYNPQPEAITFCISSFHSCKETSSVEFAEAVGASLVDMRDLCSSRQPADSKPPAPKEK ARGPTKPSNLDSSH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001170593 |
ORF Size | 1932 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001170593.1, NP_001164064.1 |
RefSeq Size | 3599 bp |
RefSeq ORF | 1935 bp |
Locus ID | 290567 |
MW | 72.4 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN217035 | Chat (untagged) - Rat choline O-acetyltransferase (Chat) |
CNY 6,270.00 |