Pja2 (NM_001277278) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Neurodap1; praja2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217038 representing NM_001277278 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACAGTATACAGAAAAAGAACCATCAGTAATGGATCAAGACTCCAGCAAGGCTGCGTGGCCTAGAG CAGCAGGAGGATACCAGACGATTACAGGCAGGAGGTACGGAAGGAGACATGCGTATGTCAGTTTTAAGCC ATGTATGACCAGGCATGAACGGAGCTTGGGTCGGGCTGGCGATGACTATGAAGTTCTGGAACTGGATGAC GTTGCCAAGGAAAACACCGCAGGTTCCAGTTCATTGGATCAAGTCCATCCTTCTTTACCCAGTGAAACTA CAGTTGAAAAAAGTGAAACAGAAATTCCTACTTGTGGTCCAGCACTGAATCAAAGCACGGAGAGCAACCC ATCCGTTGCCACAGTGTGTCATAGTGAGGAAGTCAGGGAGACCTTAGACAGCAGTACGAATCTTCAGAAT CACGCTGAGAGAGAGTGTACGCCAGCAGTTTGTAATGCCTCAAGTGTCCAGAATGGAATTGTGTTGGTTC ATACTGACTCTTATGATCCAGACAGCAAGCATGATGAGAATGACTCTCTTCAACTTTGTGCCCAAGCTGT GGAAGGTGGTAGACGTCAGAAGGTATTAGGCAATGCAGTCTTTGAGCTGGAAAATGGAGAGGTAGAGAGA TATGCTGATCTGTGTCCCTCAGTTCCCTCTCTCAGTGGTGAAATAAGGGAGGAGTCTGAAGAGCTAGGTT CAGCACTCTTAGAGAAAAATTCTGCTGGCGATGCGGAGGCTGTCCATCAGGATGGGCAGGAATTTCAGAG GTCTTCTGAAGACGGCATTGTTAGAAAGAGGCGACAAGATGATACCGATCAGGGAAGACAGACAGAAAAT TCAACTGAAGATGCAGACTGTGTTCCAGGCCATGTTGAACAAAATACTAGTGAGAGAGCCAATCACCATG GAAGTTCTCCTGAACAGGTAGTGAGGCCCAAAGTTAGAAAAGTGATCAGTTCAAGCCAGGTGGACCAAGA GAGCGGTTTTAATAGGCACGAGGCTAAGCAAAGAAGTGTTCAGAGGTGGAGAGAGGCTCTGGAAGTTGAG GAATGCAGTTCAGATGACCCTATAATCAAGTGTGACGATTATGATGGAGACCATGACTGCATGTTCCTAA CCCCATCCTACTCAAGAGTTACGCCAAGGGAAGCAGAACGTCACCGTGCGACAGCAGAAAATGGAGCCAC AGCTTCAGGAAGGCAAGAGGCTCGGGAAAATGCCTTTTGGAATGCCTGTGGAGAGTATTACCAGCTCTTT GACAAAGACGAAGACAGAGAACAGACGTCCTTGGAGGAGGGGGAGATTCCCTGGTTACAGTACAATGAGG TCAATGAGAGCAGCAGCGATGAAGGGAACGAGCCTGCCAATGAGTTTGCACAACCAGAAGCTTTCATGTT GGATGGGAACAACAACCTGGAGGACGACTCGAGCGTGAGTGAAGACCTGGATGTGGACTGGAGCCTATTT GATGGTTTTGCGGATGGACTTGGTGTTGCTGAAGCTATTTCCTACGTGGATCCTCAGTTCCTCACCTACA TGGCACTAGAAGAACGCTTAGCCCAGGCTATGGAGACTGCACTGGCACACTTAGAGTCTCTTGCTGTAGA CGTTGAGGTGGCTAACCCACCTGCCAGTAAGGAAAGCATCGACGGACTTCCAGAAACCCTTGTCCTAGAA GATCACACCGCTATTGGTCAGGAGCAGTGCTGTCCCATCTGCTGCAGTGAGTACATTAAGGATGACATCG CAACAGAGCTGCCCTGCCATCACTTCTTTCATAAGCCTTGTGTCTCCATTTGGCTACAGAAGTCCGGAAC GTGCCCTGTGTGCCGCCGCCACTTCCCACCTGCAGTGATTGACGCATCTGCAGCTGCTTCCTCTGAACCA GACCTTGATGCCTCGCCTGCAAACGACAATGCTGAGGAAGCCCCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217038 representing NM_001277278 Red=Cloning site Green=Tags(s) MSQYTEKEPSVMDQDSSKAAWPRAAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDD VAKENTAGSSSLDQVHPSLPSETTVEKSETEIPTCGPALNQSTESNPSVATVCHSEEVRETLDSSTNLQN HAERECTPAVCNASSVQNGIVLVHTDSYDPDSKHDENDSLQLCAQAVEGGRRQKVLGNAVFELENGEVER YADLCPSVPSLSGEIREESEELGSALLEKNSAGDAEAVHQDGQEFQRSSEDGIVRKRRQDDTDQGRQTEN STEDADCVPGHVEQNTSERANHHGSSPEQVVRPKVRKVISSSQVDQESGFNRHEAKQRSVQRWREALEVE ECSSDDPIIKCDDYDGDHDCMFLTPSYSRVTPREAERHRATAENGATASGRQEARENAFWNACGEYYQLF DKDEDREQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPEAFMLDGNNNLEDDSSVSEDLDVDWSLF DGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLE DHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIDASAAASSEP DLDASPANDNAEEAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277278
ORF Size	1935 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277278.1, NP_001264207.1
RefSeq Size	4528 bp
RefSeq ORF	1938 bp
Locus ID	192256
UniProt ID	Q63364
MW	71.2 kDa
Gene Summary	may play a role in synaptic communication and plasticity through the control of the formation of post synaptic density [RGD, Feb 2006]

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