Gtpbp1 (NM_001199315) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217082 representing NM_001199315 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAGCGGAGTCGCTCCCCGGTGGACTCGCCGGTGCCGGCCTCGATGTTCGCCCCCGAGCCCA GCTCTCCGGGGGCGGCCCGGGCCGCCGCGGCCGCGGCCCGACTCCACGGCGGCTTCGACTCCGACTGCAG CGAGGACGGCGAGGCGCTCAACGGCGAGCCGGAGCTGGACCTCACCAGCAAGCTGGTTCTAGTGAGCCCT ACATCAGAGCAGTATGACAGTCTACTTCGGCAGATGTGGGAGAGGATGGACGAGGGATGCGGAGAGACCA TATATGTCATTGGGCAGGGATCAGATGGGACTGAGTACGGGCTGAGTGAGGCTGACATGGAGGCCTCCTA CGCCACAGTGAAGAGCATGGCGGAGCAGATAGAGGCTGACGTCATCCTCCTGCGGGAACGTCAAGAATCG GGTGGCCGTGTGCGTGATTACTTAGTCAGGAAACGAGTCGGAGACAATGACTTCCTGGAGGTCAGGGTGG CAGTGGTGGGCAACGTGGATGCTGGCAAAAGCACACTCCTGGGAGTCCTAACACATGGGGAGCTGGACAA TGGCCGGGGCTTTGCCCGCCAAAAACTCTTTCGCCACAAACATGAGATTGAATCTGGTCGAACCAGCAGC GTGGGCAATGACATTCTGGGCTTTGACAGTGAAGGCAATGTGGTGAACAAGCCCGACAGCCACGGCGGCA GCCTTGAGTGGACAAAGATCTGTGAGAAGTCCTCCAAAGTGATCACCTTCATTGACTTGGCTGGCCACGA GAAGTACCTTAAGACCACTGTTTTTGGCATGACTGGCCATTTGCCTGACTTCTGCATGCTCATGGTGGGC AGCAATGCTGGCATCGTGGGGATGACCAAGGAGCACCTGGGCTTGGCACTGGCACTCAATGTACCTGTCT TTGTGGTTGTCACCAAGATTGACATGTGTCCTGCCAACATCCTTCAAGAAACCCTGAAGCTGCTACAGCG TCTGCTGAAGTCACCAGGCTGCCGGAAGATCCCTGTCTTGGTGCAGAGCAAAGATGATGTCATTGTCACA GCCTCCAACTTCAGCTCTGAGAGGATGTGCCCGATATTCCAGATCTCCAATGTCACAGGTGAGAACCTTG ATCTGCTCAAGATGTTCCTCAACCTCCTGTCCCCCCGCACCAGCTACAGGGAGGAAGAACCTGCTGAGTT CCAGATAGATGATACCTACTCTGTTCCTGGTGTGGGGACAGTGGTGTCTGGGACCACACTGCGGGGCCTG ATCAAACTGAATGACACACTGCTGCTGGGCCCAGACCCTTTGGGTAACTTCCTGTCCATTGCTGTCAAGT CGATCCATCGCAAGCGAATGCCTGTCAAGGAGGTACGGGGGGGCCAGACAGCCTCCTTTGCACTGAAGAA GATCAAGCGCTCATCCATCCGGAAAGGCATGGTGATGGTTTCCCCACGCCTGAATCCCCAGGCCTCCTGG GAGTTTGAGGCTGAGATTCTTGTCCTCCACCACCCCACCACAATTAGTCCACGATACCAAGCCATGGTGC ACTGTGGGAGCATCCGGCAGACAGCTACAATTCTGAGCATGGATAAAGACTGTCTGCGCACAGGAGATAA AGCCACTGTCCACTTCCGCTTCATCAAGACCCCTGAGTACCTGCACATTGACCAGCGGTTGGTGTTCCGG GAAGGTCGAACCAAGGCTGTTGGCACCATCACCAAGCTCCTCCAGACAACCAACAACTCCCCCATGAACT CCAAGCCCCAGCAGATTAAAATGCAGTCCACAAAAAAGGGTCCCCTGAGCAAACGAGAAGAAGGTGGTCC ATCTGGTGTGCCAGCGGCAGGGCCCCCCTCAACTGGAGATGAGGCTTCCTCGCTAGGGACTACACAAGCT GCCACATCCAGTGGTCTCCAGCCACAGCCCAAGCCCAGCAGTGGGGGCCGGCGACGAGGGGGCCAGCGCC ACAAGGTGAAGTCCCAGGGGGCCTGTGTGACTCCTGCCAGCGGCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217082 representing NM_001199315 Red=Cloning site Green=Tags(s) MAAERSRSPVDSPVPASMFAPEPSSPGAARAAAAAARLHGGFDSDCSEDGEALNGEPELDLTSKLVLVSP TSEQYDSLLRQMWERMDEGCGETIYVIGQGSDGTEYGLSEADMEASYATVKSMAEQIEADVILLRERQES GGRVRDYLVRKRVGDNDFLEVRVAVVGNVDAGKSTLLGVLTHGELDNGRGFARQKLFRHKHEIESGRTSS VGNDILGFDSEGNVVNKPDSHGGSLEWTKICEKSSKVITFIDLAGHEKYLKTTVFGMTGHLPDFCMLMVG SNAGIVGMTKEHLGLALALNVPVFVVVTKIDMCPANILQETLKLLQRLLKSPGCRKIPVLVQSKDDVIVT ASNFSSERMCPIFQISNVTGENLDLLKMFLNLLSPRTSYREEEPAEFQIDDTYSVPGVGTVVSGTTLRGL IKLNDTLLLGPDPLGNFLSIAVKSIHRKRMPVKEVRGGQTASFALKKIKRSSIRKGMVMVSPRLNPQASW EFEAEILVLHHPTTISPRYQAMVHCGSIRQTATILSMDKDCLRTGDKATVHFRFIKTPEYLHIDQRLVFR EGRTKAVGTITKLLQTTNNSPMNSKPQQIKMQSTKKGPLSKREEGGPSGVPAAGPPSTGDEASSLGTTQA ATSSGLQPQPKPSSGGRRRGGQRHKVKSQGACVTPASGC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199315
ORF Size	2007 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199315.1, NP_001186244.1
RefSeq Size	2647 bp
RefSeq ORF	2010 bp
Locus ID	300077
UniProt ID	D2XV59
MW	72.5 kDa
Gene Summary	Promotes degradation of target mRNA species. Plays a role in the regulation of circadian mRNA stability. Binds GTP and has GTPase activity.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.