Sema3g (NM_001100882) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CSG53; RGD1306079
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217203 representing NM_001100882 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGCTGCTCCGCTTGGGCCATCTGCTGCCTCCTGGGGAGCCTTCTGTTCCACATGGGCAGCCCGA ACCCTGGTCCCAGCACCAGTGTGCCTCGTCTGCGGCTCTCCTACAGAGATCTCCTGACTACCAACCGCTC TGCCATCTTTCTGGGTCCACGGGGCTCCCTAGACCTCCAAGTCATGTACCTGGATGAGTATCGGGACCGC CTCTTTCTGGGAGGCCGAGATGCCCTTTACTCTCTGAGACTGGATCAGGCATGGCCAGATCCCCGGGAGG TCTTGTGGCCGCCGCAGCCCGGACAGAAGGTGGAATGTGTCCGAAAGGGAAAAGACCCTTTGACCGAGTG TGCCAACTTCGTGCGGGTCTTACAACCCCACAACCGGACCCATCTGCTGGCCTGCGGCACTGGCGCCTTC CAGCCCATCTGCACCTTCATCACAGTGGGGCATCGGGGGGAGCATGTGCTCCACTTGGATCCTAGCAGCA TCGAAAATGGAAGGGGGCGTTGCCCACACGAGCCCAGCCGTCCCTTCGCCAGTACCTTTGTAGGTGGGGA GCTGTACACGGGCCTCACTGCTGATTTCCTCGGACGTGAGGCCATGATTTTCCGGAGTGGGGGTCCCCGA CCAGCCCTGCGTTCTGACTCTGACCAGAGCCTCCTACATGATCCCCGGTTTGTGATGGCTGCTCGGATCC CAGATAACTCCGACCGGGATGATGACAAAGTGTACTTCTTCTTCTCTGAGACTGTCCCTTCACCAGATGG TGGCCCAGGTCATGTCACCGTCAGCCGCGTGGGTCGAGTCTGTGTGAATGATGCTGGTGGCCAGCGGGTG CTGGTGAATAAATGGAGCACGTTCCTCAAGGCCAGGCTGGTATGCTCTGTGCCTGGCCCTGGCGGGGCTG AGACTCACTTTGACCAGCTGGAGGATGTGTTCCTGCTGTGGCCAAAGGCAGGGAAGAACCTCGAGGTGTA TGCGCTGTTCAGCACTGTCAGTGCTGTGTTCCGGGGCTTTGCTGTCTGTGTGTACCACATGGTAGACATC TGGGAGGTCTTCAACGGGCCCTTCGCTCACCGAGATGGCCCTCAACATCAGTGGGGACCCTATGGGGGCA AGGTGCCCTTCCCCCGGCCTGGTGTGTGTCCCAGCAAGATGACCGCACAGCCCGGCCGACCCTTTGGCAG CACCAAGGACTACCCGGATGAGGTGCTGCAGTTTGTCCGAGGCCACCCGCTCATGTTCCAGCCTGTGAGG CCTCGGCGTGGCCGCCCCGTCCTGGTCAAAACTCACCTGGCTCAGCAACTGTGCCAGATCGTGGTGGACA GAGTGGAGGCTGAGGATGGGACCTACGATGTCATCTTCCTAGGGACTGATTCAGGTTCTGTGCTCAAAAT CATCGCTCTCCAGGGTAGTGGCTTACCCGAACCGGAAGAGGTGGTTCTGGAGGAGCTCCAGGTGTTTAAG GTACCAACACCCATCACTGAGATGGAGATCTCTGTCAAAAGGCAAACGCTGTATGTGGGCTCTCCACTGG GCGTGGCCCGGCTGCAGCTGCACCAGTGTGAGACTTATGGCAGTGCCTGCGCTGAGTGCTGCCTGGCCCG GGACCCATACTGTGCTTGGGACGGCACTTCCTGTGCCCGCTACCGCCCCAGCTCGGGCAAGCGCAGGTTC CGACGACAGGACATCCGACATGGCAATCCTGCTGTGCAGTGTCTGGGCCAGGGCCAGAGCCAAGACAAAG CCTCGGGACTGGTGACCAGAGTCTTTGGCACAGAGCACAACAGTACCTTCCTTGAGTGCCGGCCCAAGTC TCCCCAGGCTGCAGTGCGCTGGTTCTTACAAAGGCCAGGGGATAAGGGGGCTGACCAGGTGAAGACAGAT GAGCGAGTTGTGCAGACGGACCAGGGGCTTCTGTTCCGAAGGCTCAGCCGCCTTGATGCAGGGAACTACA CCTGTACCACTCTGGAGCACGGCTTCTCCCAGACCGTGGTTCGTTTTGCCCTGGAGGTGATTGCGGCTGT GCAGCTGGACAGCCTGTTCCTTCGGGAGCCAAGGCTGGAGGAGCCCTCAGCCTGGCGAAGTGTGGCTTCT GCCTCACCCAAGACTTGGTATAAGGACATCCTTCAGCTCACTGGTTTCGCCAACCTGCCCCGCGTGGATG AGTACTGCGAACGTGTGTGGTGCAGGGGTGTCGGGGAGCGCTCGGGCTCCTTCCGCGGAAAGGGAAAGCA GGCGAAGGGCAAGAGCTGGGCGGGGCTAGAGCTGGGCAGGAAGATGAAGAGCCGGGTGCAGGCTGAGCAC AATCGGACCCCCCGGGAGGTAGAAGCCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217203 representing NM_001100882 Red=Cloning site Green=Tags(s) MARCSAWAICCLLGSLLFHMGSPNPGPSTSVPRLRLSYRDLLTTNRSAIFLGPRGSLDLQVMYLDEYRDR LFLGGRDALYSLRLDQAWPDPREVLWPPQPGQKVECVRKGKDPLTECANFVRVLQPHNRTHLLACGTGAF QPICTFITVGHRGEHVLHLDPSSIENGRGRCPHEPSRPFASTFVGGELYTGLTADFLGREAMIFRSGGPR PALRSDSDQSLLHDPRFVMAARIPDNSDRDDDKVYFFFSETVPSPDGGPGHVTVSRVGRVCVNDAGGQRV LVNKWSTFLKARLVCSVPGPGGAETHFDQLEDVFLLWPKAGKNLEVYALFSTVSAVFRGFAVCVYHMVDI WEVFNGPFAHRDGPQHQWGPYGGKVPFPRPGVCPSKMTAQPGRPFGSTKDYPDEVLQFVRGHPLMFQPVR PRRGRPVLVKTHLAQQLCQIVVDRVEAEDGTYDVIFLGTDSGSVLKIIALQGSGLPEPEEVVLEELQVFK VPTPITEMEISVKRQTLYVGSPLGVARLQLHQCETYGSACAECCLARDPYCAWDGTSCARYRPSSGKRRF RRQDIRHGNPAVQCLGQGQSQDKASGLVTRVFGTEHNSTFLECRPKSPQAAVRWFLQRPGDKGADQVKTD ERVVQTDQGLLFRRLSRLDAGNYTCTTLEHGFSQTVVRFALEVIAAVQLDSLFLREPRLEEPSAWRSVAS ASPKTWYKDILQLTGFANLPRVDEYCERVWCRGVGERSGSFRGKGKQAKGKSWAGLELGRKMKSRVQAEH NRTPREVEAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100882
ORF Size	2340 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100882.1, NP_001094352.1
RefSeq Size	3626 bp
RefSeq ORF	2343 bp
Locus ID	290562
MW	86.9 kDa

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