Zhx2 (NM_001271056) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217250 representing NM_001271056 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGCAAACGGAAATCGACAACGCCGTGCATGGTCCGGACATCACAGGTGGTAGAACAAGACGTGC TGGAGGAGGCAGACAGGGCCAAAGACAAAGGACTGGGCGTGCCACCGTCCGATGTGAGCAAGGAGAGGTG GGCAGCAGAGCCGGAACCCTCGTCCAAAGAAAGCGAGGTGGTGGAGGTGAGATCGGTGGGGGAGAGCCAG TCCAAGAAACTCCAGGGTGGGTATGAGTGCAAGTACTGCCCTTACTCCACGCAAAACCTGAATGAGTTCA CGGAGCACGTGGATATGCAGCACCCCAACGTGATTCTCAACCCCCTCTACGTATGTGCCGAATGTAACTT CACAACCAAAAAGTATGACTCCCTGTCTGACCACAACTCCAAGTTCCATCCCGGGGAGACCAACTTCAAG CTGAAGTTAATCAAGCGTAATAATCAGACGGTCCTAGAGCAGTCCATCGAGGCCACCAACCACGTTGTGT CCATCACTGCCAGTGCTCCTGGAAGTAGTGATAATGACCCCGGGGTCTCAGTAGGTAAGACTGCCACGGT GAAGACAGGAAAGCAGAAGGCAGATGCCAAGAAAGTGCCCAAGAAGCCTGATGAGGCTGCCCCAGACAAC CATATGGAAGGGACTGCCCGCCTGGTGACAGACACAGCCGAGATCCTGTCCAGACTTGGAAGTGTGGAGC TCCTTCACGATTCACTGGGACACGTCATGCCTTCCGTACAGCTGCCACCAAATATCAACCTTGTCCCCAA GGTCCCCGTCCCGCTGAATACTACCAAATACAACTCTGCCCTGGACACAAATGCTACCATGATCAACTCC TTCAACAAGTTCCCTTACCCCACCCAGGCTGAGCTCTCCTGGCTGACCGCAGCCTCCAAACACCCAGAGG AGCATATCCGAATCTGGTTTGCCACCCAGCGTTTAAAGCATGGTATCAGCTGGTCCCCAGAGGAGGTGGA GGAGGCCCGAAAGAAGATGTTTAACGGCACGATTCAGTCAGTACCCCCAACTATCACTGTGCTTCCTGCA CAGCTGACCCCCACAAAAGTGTCACAGCCCATCCTCCAGACAGCCCTACCGTGCCAGATCCTTGGCCAGC CCAGCCTGGTGCTGACTCAAGTGACAAGTGGGTCAACAGCTGTCTCGTGTTCTCCCATCACACTTGCCGT GGCTGGAGTGACCAACCATGGCCAGAAGAGACCTCTGGTGACTCCTCAAGCTGCCCCTGAGCCGAAGCGT CCACACATCGCCCAGGTGCCAGAACCTCCACCCAAGGTGGCCAATACCCCACTCACGCCAGCTAGTGATC GCAAGAAGACCAAGTTACAGATCGCCCACCTCAAGGCAAGCTTCTTACAGAGCCAGTTCCCTGACGATGC CGAGGTGTACCGGCTCATTGAGGTGACAGGCCTTGCCAGGAGTGAGATCAAGAAGTGGTTCAGCGACCAT CGCTACCGGTGTCAGAGAGGCATTGTCCACATCACCAGCGAATCCCTTGCCAAAGACCAGATGGCCATTA CTGGCACCCGACACGGTCGCACCTACCACGTATACCCAGACTTTGCCGCCCAGAAGTTCAAAGAGAAAAG CCAGGGACAGTTGAAAACCCTGGAAGACAGCTTTCTGAAAAGCTCTTTTCCCACCCAGGCAGAAGTAGAA CGGCTGAGGGTGGAAACCAAGCTAAGCAGGAGGGAAATCGACTCCTGGTTTTCCGAGAGGCGAAAGCTTC GAGACAGCATGGAGCAGGCTGTCTTGGATTCCATGGGGTCTGGCAAAAAGGGCTCGGATGTGGTAGCCCC CAATGGTGCTCTATCCCGACTTGATCAGCTCTCTGGTGCCCAGTTAGCTGGTCCTTTGCCCAGTCCTTCA TCAGCAGTTGTACAAAATCAAGAGCAGGTTCACCTACTCAGGAGCACCTTTGCAAGAACCCAGTGGCCCA CTCCTCAGGAGTACGACCAGTTAGCTGCCAAGACTGGCCTGGTCCGAACTGAGATTGTGCGCTGGTTCAA GGAGAACAGATGCTTACTAAAAACTGGAACGTTGAGCTGGTTGGAGCAGTATCAGAGGCATCACCTGTCA GATGACCATGGCCACGATGTCGCATCAAGAAGAGCGACAAAACACGTTGCTGAGAGCCCAAAGAATGGAA GCGAGGTGGCTCACCAGTATGCCAAGGACCCCAAAGCCCTCGGCGAGGAGGAATCCGAGAAGCTGGTCCC CAGGGTGAAATTGGTCGGCGATCCATCCAAGGACTGTCTGGCGGGCAAGCCCTCAGAGGCCACCTCAGAC AGATCCGAGGGTAGCCGAGATGGCCAGGGCAGCGAGGAAAACGAGGAGTCAGGCATCGTGGACTTTGTGG AGGTGACGGTTGGAGAGGAGGACGCCATCTCAGAGAAGTGGGGTAGCTGGAGTCAGAGAGTAGCAGAAGG CACAGTAGAAAGAGCGGACTCCGACTCGGACAGCACCCCTGCAGAGGCCGGCCAAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217250 representing NM_001271056 Red=Cloning site Green=Tags(s) MASKRKSTTPCMVRTSQVVEQDVLEEADRAKDKGLGVPPSDVSKERWAAEPEPSSKESEVVEVRSVGESQ SKKLQGGYECKYCPYSTQNLNEFTEHVDMQHPNVILNPLYVCAECNFTTKKYDSLSDHNSKFHPGETNFK LKLIKRNNQTVLEQSIEATNHVVSITASAPGSSDNDPGVSVGKTATVKTGKQKADAKKVPKKPDEAAPDN HMEGTARLVTDTAEILSRLGSVELLHDSLGHVMPSVQLPPNINLVPKVPVPLNTTKYNSALDTNATMINS FNKFPYPTQAELSWLTAASKHPEEHIRIWFATQRLKHGISWSPEEVEEARKKMFNGTIQSVPPTITVLPA QLTPTKVSQPILQTALPCQILGQPSLVLTQVTSGSTAVSCSPITLAVAGVTNHGQKRPLVTPQAAPEPKR PHIAQVPEPPPKVANTPLTPASDRKKTKLQIAHLKASFLQSQFPDDAEVYRLIEVTGLARSEIKKWFSDH RYRCQRGIVHITSESLAKDQMAITGTRHGRTYHVYPDFAAQKFKEKSQGQLKTLEDSFLKSSFPTQAEVE RLRVETKLSRREIDSWFSERRKLRDSMEQAVLDSMGSGKKGSDVVAPNGALSRLDQLSGAQLAGPLPSPS SAVVQNQEQVHLLRSTFARTQWPTPQEYDQLAAKTGLVRTEIVRWFKENRCLLKTGTLSWLEQYQRHHLS DDHGHDVASRRATKHVAESPKNGSEVAHQYAKDPKALGEEESEKLVPRVKLVGDPSKDCLAGKPSEATSD RSEGSRDGQGSEENEESGIVDFVEVTVGEEDAISEKWGSWSQRVAEGTVERADSDSDSTPAEAGQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271056
ORF Size	2508 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271056.1, NP_001257985.1
RefSeq Size	2855 bp
RefSeq ORF	2511 bp
Locus ID	314988
UniProt ID	Q80VX4
MW	92.1 kDa
Gene Summary	putative transcriptional repressor [RGD, Feb 2006]

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