Adamts4 (NM_023959) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217263 representing NM_023959 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCAATCCATCCCAGCTGCAGCACTGGTACCATGTCCAGGATGGGCTTGCATCCCAGGAGGGGCT TGACTGGGCACAGACTGCGAAGACTCCAACCCTGCCTGCCGCCCCGCACTGTGCCTTTCTCTGGTTTGGA GCGGTGGCAGCAGCTGCTGCTGCTGGCCTCCCTCCTGCCTTTAGCCTGGTCAGCCAGCCCCCTCCCCCGA GAGGAGGAGATCGTGTTTCCAGAGAAGCTCAACGGCAGTAGTATCATACCTGGCTCAGGCGTCCCTGCCA GGCTGCTGTACCGATTACCAGCCTTTGGGGAGATATTGTTACTAGAACTAGAGCAGGATCCTGGGGTGCA GGTAGAGGGGCTGACAGTACAGTACCTGGGCCAGGCACCTGAGATGCTGGGTGGGGCAGAGCCAGGTACC TACCTGACTGGCACCATCAATGGAGATCCGGAGTCGGTGGCGTCTCTGCACTGGGACAGGGGAGCCCTAT TGGGGGTATTGCAGTACCGGGGGGCCGAACTCCACCTCCAGCCTCTGGAAGGAGGCGCCCTTAACTCTGC TGGGGGACCAGGGGCTCACATCCTACGCCGGAAGAGTCCTGCCAGCAACCGAGGTCCCATATGCAACGTC AAGGCTCCTTCTGGGAGCCCCAGTCCCATTTCCCGCAGAAACAAGCGCTTCGCTTCGCTGAGTAGATTCG TGGAGACACTGGTGGTGGCAGATGACAAGATGGCAGCATTCCACGGGGCAGGGTTAAAGCACTACCTGCT GACGGTCATGGCCGCCGCTGCCAAAGCCTTTAAGCATCCAAGCATCCGAAACCCTGTCAACTTGGTGGTG ACTCGTCTAGTAATCCTGGGGTCGGGCCAGGAAGTGCCCCAAGTGGGGCCAAGTGCCGCCCAGACCCTAC GCAGCTTCTGCACCTGGCAGAAAGGCCTAAACCCTCCGAACGACTCAGATCCTGACCACTTTGACACGGC CATTCTGTTCACCCGGCAGGACCTGTGTGGGGTCTCCACCTGTGACGCTCTGGGTATGGCTGGTGTGGGC ACAGTGTGTGACCCAGCTAGGAGCTGTGCTATTGTGGAGGACGATGGGCTCCAGTCGGCCTTCACCGCCG CTCATGAACTGGGCCATGTCTTCAACATGCTCCATGATAACTCCAAACCGTGTGCTAATTTGAATGGGCA GGGGAGTTCCTCTCGCCATGTCATGGCTCCTGTCATGGCTCACGTGGACCCTGAAGAGCCCTGGTCCCCC TGCAGTGCCCGATTCATCACTGACTTCCTGGACAATGGCTATGGACACTGCCTCTTAGACAAACCAGAGG CTCCCCTGCATCTGCCAGTGACTTTTCCTGGCAAGGACTATGATGCTGACCGCCAGTGCCAACTGACCTT TGGTCCTGACTCAAGCCATTGTCCACAGCTGCCACCGCCCTGTGCTGCCCTCTGGTGCTTTGGCCACCTC AATGGCCATGCCATGTGTCAGACTAAGCACTCACCCTGGGCCGATGGTACTCCCTGCGGACCTGCACAGG CCTGTATGGGTGGCCGCTGTCTCCACGTGGACCAGCTCAAGGACTTCAATATTCCTCAGGCTGGAGGCTG GGGTCCCTGGGGACCATGGGGTGACTGCTCCAGGACTTGTGGAGGTGGTGTCCAATTCTCCTCCCGGGAC TGCACAAAGCCTGTCCCCCGGAATGGTGGAAAGTATTGTGAGGGCCGCCGTACCCCGTTCCGCTCCTGTA ACACTAAGAACTGCCCACATGGCTCAGCATTGACCTTCCGTGAAGAGCAGTGTGCTGCCTACAACCACCG AACCGACCTCTTCAAGAGTTTTCCGGGGCCCATGGACTGGGTTCCGCGCTACACAGGTGTAGCCCCTCGA GACCAGTGCAAACTCACCTGCCAGGCCCGGGCACTGGGCTACTACTATGTACTGGAGCCACGGGTGGCAG ATGGAACTCCCTGTTCCCCAGACTCTTCCTCTGTCTGTGTCCAGGGCCGCTGTATCCACGCTGGCTGTGA TCGAATCATTGGCTCCAAAAAGAAATTTGACAAGTGCATGGTGTGCGGTGGGAATGGCTCTAGCTGCAGC AAGCAGTCAGGCTCCTTCAAAAAATTCAGGTACGGATACAGCGATGTGGTCACGATCCCTGCGGGACGCA CCCATATCCTTGTACGGCAGCAGGGAGGATCTGGCCTCAAGAGCATCTACCTGGCCCTGAAGCTAGCAGA TGGTTCTTACGCCCTCAATGGTGAATACACGCTGATGCCCTCCTCAACAGATGTGGTACTTCCTGGGGCA GTCAGCTTGCGCTATAGCGGACGCACGGCAGCCTCAGAGACACTGTCTGGACATGGGCCGCTGGCCCAGC CCTTGACGCTGCAAGTTCTGGTGGCTGGCAACCCACAGAATGTACGTCTGCGGTACAGTTTCTTTGTCCC GCGGCCAGTCCCTTCAACGCCACGCCCTCCTCCCCAAAACTGGCTGCAACGCAGGGCAGAGATTCTGGAG ATCCTTCGGAAGCGAACCTGGGCAGGCCGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217263 representing NM_023959 Red=Cloning site Green=Tags(s) MASIHPSCSTGTMSRMGLHPRRGLTGHRLRRLQPCLPPRTVPFSGLERWQQLLLLASLLPLAWSASPLPR EEEIVFPEKLNGSSIIPGSGVPARLLYRLPAFGEILLLELEQDPGVQVEGLTVQYLGQAPEMLGGAEPGT YLTGTINGDPESVASLHWDRGALLGVLQYRGAELHLQPLEGGALNSAGGPGAHILRRKSPASNRGPICNV KAPSGSPSPISRRNKRFASLSRFVETLVVADDKMAAFHGAGLKHYLLTVMAAAAKAFKHPSIRNPVNLVV TRLVILGSGQEVPQVGPSAAQTLRSFCTWQKGLNPPNDSDPDHFDTAILFTRQDLCGVSTCDALGMAGVG TVCDPARSCAIVEDDGLQSAFTAAHELGHVFNMLHDNSKPCANLNGQGSSSRHVMAPVMAHVDPEEPWSP CSARFITDFLDNGYGHCLLDKPEAPLHLPVTFPGKDYDADRQCQLTFGPDSSHCPQLPPPCAALWCFGHL NGHAMCQTKHSPWADGTPCGPAQACMGGRCLHVDQLKDFNIPQAGGWGPWGPWGDCSRTCGGGVQFSSRD CTKPVPRNGGKYCEGRRTPFRSCNTKNCPHGSALTFREEQCAAYNHRTDLFKSFPGPMDWVPRYTGVAPR DQCKLTCQARALGYYYVLEPRVADGTPCSPDSSSVCVQGRCIHAGCDRIIGSKKKFDKCMVCGGNGSSCS KQSGSFKKFRYGYSDVVTIPAGRTHILVRQQGGSGLKSIYLALKLADGSYALNGEYTLMPSSTDVVLPGA VSLRYSGRTAASETLSGHGPLAQPLTLQVLVAGNPQNVRLRYSFFVPRPVPSTPRPPPQNWLQRRAEILE ILRKRTWAGRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_023959
ORF Size	2553 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_023959.1, NP_076449.1
RefSeq Size	3339 bp
RefSeq ORF	2556 bp
Locus ID	66015
UniProt ID	Q9ESP7
MW	91.9 kDa
Gene Summary	a disintegrin and metalloproteinase; may play a role in the progression of Alzheimers disease [RGD, Feb 2006]

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