Rad54b (NM_001191755) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Fsbp; RGD1306507
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217301 representing NM_001191755 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACGTTCTGCAGCACCAAGCCAGGTGCGGGGAAATTCCTCTAAAAAAGCAAGATTTGTACCTCCAG GAAGAAGTACTACAGGTGTGAGTAAGGAGATTTCGATGAGTCCAGAGGCAAAACTGGTTCAGGGCGCCCA CCAGTCACAAGGGGATGCAGGTGTGTGCAGTTCCAATCCTTGGCTCACTGAGGGAAGCCCTGTGGAGATG GGTGACGGGGCTAGAGTAGATCCGCTTTCTCCAGTTCACTTGGCCTCTAAAGAAATCATAGAGTCCAAGG CACAAGAAGAGGAAGCTAGCAGCTTGCTTAAATATTTCAGTGTCGTTTGGTGTAAAGCTTCAAAGAAAAA ACACAAGAAATGGGAAGGCGATGCTATTCTTATTGTAAAAGGAAGGTCGTTCATATTGAAAGATATGGAA GGAAAAGATATTGGAAGAGGCATGGGATATAAATTCAAAGAGCTTGAAAATGTAGAAGAAGGCCAAACAT TGATGCTTGGTGGGAAAGAAATAGAGATCCTGGGTACAGTCTCTGCTGAGGACTTCAATAGTGGCAAGTG TTTTCAGGCTGGAAGCGGAAGTCCTGCCGTCCCAAGTTCTCAGGCTGCCAGGAAATGCTTTTCTAACCCT TTCAAGAGTGTGTGTCAGTCAACTCAAGCAAGGAGAGACAGAGGGAATGACTGGCAAAATTGCAAACCTC GCTATGACCCATACACACCAAATGCCCTTGTTATGCCTCGACCAGACAAGAGTCACCAGTGGGCGTTTAA TAGGAGCTGCCTTCCTACTGTGGATGTGGTGATAGACCCTCACCTTGTACGTCACCTCCGACCACACCAG AAAGATGGGGTCGCCTTCCTCTATGAGTGTGTGATGGGAATGAGAGCAGTTGGCAAATGTGGTGCCATTC TTGCTGATGAGATGGGATTAGGGAAAACCCTGCAGTGTATCTCGCTCATCTGGACCCTCCAGTGTCAGGG GCCTTACGGGGGCAAGCCAATAGTAAAGAGGACACTTATTGTTACCCCTGGAAGCTTGGTGAATAATTGG AGAAAAGAATTTCAAAAATGGCTGGGAAGCGAAAGGATCAAGATATTCACTGTTGATCAGGACCATAAAG TCGAGGAGTTTATCAATTCTGCATTTCACTCTGTTCTTATCATCAGTTATGAAATGTTACTTCGTTCTCT AGATCGAATTAAGACCATAACATTTGGCCTTCTGATTTGTGATGAGGGGCACCGGTTGAAGAACAGTGGT ATTAAGACAACAGCAGCCCTTTCTAGCCTCTCTTGTGAGAAGAGAGTAATTCTGACTGGTACCCCAGTTC AGAATGATTTGCAAGAGTTTTTTGCATTAGTTGATTTTGTTAATCCAGGAATCTTAGGGTCTCTGTCATC TTATAGGAGGATATATGAAGAGCCCATTGTTATGTCAAGAGAGCCTTCTGCTTCCAAGGAAGAGAGAGAG TTAGGGGAGAGAAGAACAACTGAGCTCACACGCCTCACTGGATGTTTCATCCTTAGAAGAACTCAGGAGG TCATCAATAAATATCTCCCCCCTAAGATAGAGAACGTAGTCTTTTGCCGACCAGGAGCACTGCAGATTGA GCTTTACCGAAAGCTGCTGAGTTCTCAATCTGTCAGGTTCTGTCTGCAAGGCCTTTTGGGAAATAGTGCT CACCTCATTTGCATAGGCGCCCTGAAGAAGCTATGCAATCATCCTCGCCTCTTGTTCAGCTTTGTGAAGG GTAAAGAATTTAACTCGAGTCGTGATGAAAATGAAGAAAGGAGCCTTTGCCAAGGCTTGCTGACTGTGTT CCCTGCTGGCTACAACCTGCTGCAGCTCTCCGAGAGCGAGTCAGGGAAACTACAGGTGTTAGTAAAGCTC CTGGCGGCGATCTCTGAACTTCGTCCCACTGAAAAAGTGATTTTGGTATCCAACTACAGACAAACCTTGA ATCTTTTAGAAGAAGTATGCAAGTGTCATGGATATGCTTGTGCAAGACTTGATGGACAAACACCAGTCTC TCAAAGACAGCAAATTGTTGATAATTTCAATAGTAAATACTCTACTGATTTTATTTTTTTATTAAGTTCA AAAGCTGGTGGTGTGGGACTTAATCTTATTGGAGGATCTCACTTAATTCTTTATGATATTGATTGGAACC CAGCTACTGACATCCAGGCCATGGCTAGAGTGTGGAGAGACGGCCAGAAACACCCTGTCCATGTTTACAG ACTCCTGACTACAGGTACAATAGAGGAAAAGATCTATCAAAGGCAGATCAGTAAGCAAGGCCTTTCTGGG GCGGTTGTAGACCTAACCAGATCATCCGAACAGATCCAGTTTTCAGTGGAAGAGCTTAAAAACTTGTTCA CATTACATGAAAATTCACATTGTGTTACTCATGACCTGCTTGACTGTGAGTGCACAGGAGAGAAAGATCA CTCTGATGATGCGTCGGAAAACCCTGTCACCTCTAGACAGTGTCAGCTTGGCCCTCAGAAGTCCAGCTCC TCGAGACCTCTGTCCATGTCACAGCTGAAGCAGTGGAAACATTTTTCAGGAGACCATTTAAATCTCCCAG ACCCATTTCTCGAAAGAATAAGGGAAAATGTGTCCTTCTTTTTCCAGAATATAACCAACCAAGCTCCTGC CATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217301 representing NM_001191755 Red=Cloning site Green=Tags(s) MRRSAAPSQVRGNSSKKARFVPPGRSTTGVSKEISMSPEAKLVQGAHQSQGDAGVCSSNPWLTEGSPVEM GDGARVDPLSPVHLASKEIIESKAQEEEASSLLKYFSVVWCKASKKKHKKWEGDAILIVKGRSFILKDME GKDIGRGMGYKFKELENVEEGQTLMLGGKEIEILGTVSAEDFNSGKCFQAGSGSPAVPSSQAARKCFSNP FKSVCQSTQARRDRGNDWQNCKPRYDPYTPNALVMPRPDKSHQWAFNRSCLPTVDVVIDPHLVRHLRPHQ KDGVAFLYECVMGMRAVGKCGAILADEMGLGKTLQCISLIWTLQCQGPYGGKPIVKRTLIVTPGSLVNNW RKEFQKWLGSERIKIFTVDQDHKVEEFINSAFHSVLIISYEMLLRSLDRIKTITFGLLICDEGHRLKNSG IKTTAALSSLSCEKRVILTGTPVQNDLQEFFALVDFVNPGILGSLSSYRRIYEEPIVMSREPSASKEERE LGERRTTELTRLTGCFILRRTQEVINKYLPPKIENVVFCRPGALQIELYRKLLSSQSVRFCLQGLLGNSA HLICIGALKKLCNHPRLLFSFVKGKEFNSSRDENEERSLCQGLLTVFPAGYNLLQLSESESGKLQVLVKL LAAISELRPTEKVILVSNYRQTLNLLEEVCKCHGYACARLDGQTPVSQRQQIVDNFNSKYSTDFIFLLSS KAGGVGLNLIGGSHLILYDIDWNPATDIQAMARVWRDGQKHPVHVYRLLTTGTIEEKIYQRQISKQGLSG AVVDLTRSSEQIQFSVEELKNLFTLHENSHCVTHDLLDCECTGEKDHSDDASENPVTSRQCQLGPQKSSS SRPLSMSQLKQWKHFSGDHLNLPDPFLERIRENVSFFFQNITNQAPAI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191755
ORF Size	2664 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191755.1, NP_001178684.1
RefSeq Size	2806 bp
RefSeq ORF	2667 bp
Locus ID	313063
MW	99.2 kDa

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