Atp12a (NM_001301664) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217338 representing NM_001301664 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGGGGCTTTTCCATCCTCCTGTGGATAGGTGCTGCCTTGTGCTGGATCGCATTTGTGATCCAGT ACGTCAACAATTCCGCATCCCTAGACAACGTCTACTTGGGCGCCATACTTGTCCTGGTTGTTATTTTAAC GGGGATTTTTGCTTATTACCAAGAAGCCAAAAGCACCAACATCATGGCCAGCTTTAGTAAGATGATCCCC CAGCAAGCTCTTGTCATCCGAGATGCCGAAAAGAAGGTCATTTCAGCAGAGCAACTGGTGGTGGGGGACG TTGTGGAGATTAAAGGAGGGGACCAGATCCCTGCAGACATCAGACTGGTATTTTCCCAAGGGTGCAAAGT AGATAACTCCTCTCTCACTGGGGAATCTGAGCCCCAGGCTCGTTCAACTGAGTTTACCCATGAAAACCCC TTGGAAACCAAGAACATAGGCTTCTATTCCACAACCTGCCTGGAAGGTACAGCAACTGGCATTGTCATCA ACACGGGTGACCGTACCATCATCGGGCGCATAGCCTCCTTGGCTTCAGGCGTTGGGAGTGAGAAAACACC CATTGCCATTGAGATCGAGCATTTTGTCCATATTGTGGCAGGAGTGGCTGTGTCCATTGGCATCATTTTC TTCATCACCGCCGTGTGCATGAAGTACTATGTCCTCGACGCCATCATCTTCCTCATTAGCATCATTGTGG CTAACGTGCCAGAGGGCCTCCTGGCTACTGTCACTGTTACCCTGTCACTGACGGCAAAACGGATGGCCAA GAAGAACTGCCTGGTAAAGAATCTGGAGGCAGTGGAGACTCTTGGCTCCACCTCCATCATCTGCTCAGAC AAGACGGGGACCCTGACCCAGAACAGGATGACTGTGGCTCACTTGTGGTTTGACAATCAGATCTTTGTGG CTGATACTAGTGAAAACCAAACAAAACAAGCCTTTGACCAAAGCTCTGGAACCTGGGCTTCCTTGTCCAA GATAATAACACTGTGTAACCGAGCAGAGTTCAGGCCAGGCCAGGAGAGTGTCCCCATCATGAAGAGAACT GTGGTTGGAGACGCCTCAGAAACTGCTCTGCTAAAGTTCTCAGAGGTCATTTTGGGTGACGTGATGGGAA TTAGGAAAAGAAACCACAAAGTGGCTGAAATTCCTTTTAATTCTACCAACAAATTTCAGCTCTCCATACA TGAGACAGAGGACCCTAATGACAAACGCTTCCTCGTGGTGATGAAAGGAGCCCCTGAGAGGATCTTAGAG AAGTGCAGCACCATCATGATCAATGGCCAGGAGCAGCCACTGGACAAGAGCTCTGCTGATAGCTTCCACA CAGCCTACATGGAGCTGGGCGGTCTGGGCGAGCGTGTGTTGGGTTTCTGTCACCTCTACTTGCCAGCAGA GCAGTTCCCCCAAAGTTACATCTTTGATGTCGACTCCGTAAACTTTCCCACTTCCAACTTCTGCTTTGTG GGGCTTCTGTCCATGATCGACCCTCCTCGGTCTACTGTGCCAGACGCAGTCTCCAAATGTCGGAGTGCCG GGATCAAGGTTATCATGGTCACAGGTGATCACCCTATCACCGCCAAAGCTATTGCCAAGAGTGTAGGAAT CATATCGGCCAACAACGAGACAGTGGAAGACATCGCGAAACGCCGCAATATCGCTGTGGAGCAGGTCAAC AAAAGAGAAGCTAAAGCCGCTGTGGTGACTGGCATGGAGCTGAAGGACATGACCCCAGAACAACTGGATG AGCTTTTGACCAACTACCAAGAAATCGTATTTGCCCGGACATCACCCCAGCAGAAGTTGATCATTGTGGA GGGCTGTCAGAGGCAGGACGCCATCGTTGCCGTGACGGGTGATGGAGTGAACGACTCTCCAGCACTGAAG AAGGCAGACATCGGGATTGCTATGGGGATAGCAGGTTCTGACGCTGCTAAGAACGCAGCTGACATGGTCT TACTAGACGACAACTTCGCGTCTATAGTCACAGGGGTGGAGGAAGGTCGCCTGATCTTCGACAACCTGAA GAAAACCATTGCGTACACCCTGACAAAGAACATCGCCGAGCTCTGCCCTTTTTTGATCTACATCGTTGCT GGGCTCCCCCTGCCTATTGGAACCATCACTATCCTATTCATCGACCTGGGCACAGACATTATCCCCTCCA TTGCCTTGGCCTATGAGAAAGCAGAAAGTGACATTATGAATAGGAAACCTCGCCACAAGAAGAAAGACAG ACTGGTGAACACGCAGCTGGCCATCTACTCTTACCTTCACATTGGTCTCATGCAAGCTCTGGGAGGTTTC CTCGTCTATTTCACTGTCTATGCACAGCAGGGCTTTTGGCCCACTTCTCTCATCAACCTGAGGGTAGCAT GGGAAACAGATGACATAAATGACTTGGAAGACAGCTACGGGCAGGAATGGACAAGGTATCAGAGAAAATA CCTGGAATGGACAGGCTCAACGGCTTTCTTTGTTGCCATCATGATCCAACAAATTGCGGATCTGATCATC AGGAAAACCCGGAGGAATTCCATCTTTCAGCAGGGGCTCTTCAGAAATAAAGTCATCTGGGTGGGGATCG CCTCACAGGTCATCGTTGCACTGATCCTCTCCTATGGTCTCGGGAGTGTCCCAGCTCTGAGCTTTACCAT GCTCAGGGTTCAGTACTGGTTTGTGGCTGTGCCACATGCCATTTTGATTTGGGTGTATGACGAGATGCGA AAGTTGTTCATCAGGCTCTACCCTGGAAGCTGGTGGGATAAGAACATGTATTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217338 representing NM_001301664 Red=Cloning site Green=Tags(s) MVGGFSILLWIGAALCWIAFVIQYVNNSASLDNVYLGAILVLVVILTGIFAYYQEAKSTNIMASFSKMIP QQALVIRDAEKKVISAEQLVVGDVVEIKGGDQIPADIRLVFSQGCKVDNSSLTGESEPQARSTEFTHENP LETKNIGFYSTTCLEGTATGIVINTGDRTIIGRIASLASGVGSEKTPIAIEIEHFVHIVAGVAVSIGIIF FITAVCMKYYVLDAIIFLISIIVANVPEGLLATVTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSD KTGTLTQNRMTVAHLWFDNQIFVADTSENQTKQAFDQSSGTWASLSKIITLCNRAEFRPGQESVPIMKRT VVGDASETALLKFSEVILGDVMGIRKRNHKVAEIPFNSTNKFQLSIHETEDPNDKRFLVVMKGAPERILE KCSTIMINGQEQPLDKSSADSFHTAYMELGGLGERVLGFCHLYLPAEQFPQSYIFDVDSVNFPTSNFCFV GLLSMIDPPRSTVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKSVGIISANNETVEDIAKRRNIAVEQVN KREAKAAVVTGMELKDMTPEQLDELLTNYQEIVFARTSPQQKLIIVEGCQRQDAIVAVTGDGVNDSPALK KADIGIAMGIAGSDAAKNAADMVLLDDNFASIVTGVEEGRLIFDNLKKTIAYTLTKNIAELCPFLIYIVA GLPLPIGTITILFIDLGTDIIPSIALAYEKAESDIMNRKPRHKKKDRLVNTQLAIYSYLHIGLMQALGGF LVYFTVYAQQGFWPTSLINLRVAWETDDINDLEDSYGQEWTRYQRKYLEWTGSTAFFVAIMIQQIADLII RKTRRNSIFQQGLFRNKVIWVGIASQVIVALILSYGLGSVPALSFTMLRVQYWFVAVPHAILIWVYDEMR KLFIRLYPGSWWDKNMYY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301664
ORF Size	2784 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301664.1, NP_001288593.1
RefSeq Size	4160 bp
RefSeq ORF	2787 bp
Locus ID	171028
MW	102.5 kDa
Gene Summary	may act as a H+,K(+)-ATPase in distal colon; may also play a role in K+ absorption and H+ secretion in the distal nephron [RGD, Feb 2006]

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