Grin1 (NM_001270602) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GluN1; NMDAR1; NR1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217364 representing NM_001270602 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCACCATGCACCTGCTGACATTCGCCCTGCTTTTTTCCTGCTCCTTCGCCCGCGCCGCCTGCGACC CCAAGATCGTCAACATCGGCGCGGTGCTGAGCACGCGCAAGCATGAACAGATGTTCCGCGAGGCAGTAAA CCAGGCCAATAAGCGACACGGCTCTTGGAAGATACAGCTCAACGCCACTTCTGTCACCCACAAGCCCAAC GCCATACAGATGGCCCTGTCAGTGTGTGAGGACCTCATCTCTAGCCAGGTCTACGCTATCCTAGTTAGCC ACCCGCCTACTCCCAACGACCACTTCACTCCCACCCCTGTCTCCTACACAGCTGGCTTCTACAGAATCCC TGTCCTGGGACTGACTACCCGAATGTCCATCTACTCTGACAAGAGTATCCACCTGAGTTTCCTTCGCACG GTGCCGCCCTACTCCCACCAGTCCAGCGTCTGGTTTGAGATGATGCGAGTCTACAACTGGAACCACATCA TCCTGCTGGTCAGCGACGACCACGAGGGACGGGCAGCGCAGAAGCGCTTGGAGACGTTGCTGGAGGAACG GGAGTCCAAGAGTAAAAAAAGGAACTATGAAAACCTCGACCAACTGTCCTATGACAACAAGCGCGGACCC AAGGCAGAGAAGGTGCTGCAGTTTGACCCAGGAACCAAGAATGTGACGGCTCTGCTGATGGAGGCCCGGG AACTGGAGGCCCGGGTCATCATCCTTTCTGCAAGCGAGGACGACGCTGCCACAGTGTACCGCGCAGCCGC AATGCTGAACATGACGGGCTCTGGGTACGTGTGGCTGGTCGGGGAACGCGAGATCTCTGGGAACGCCCTG CGCTACGCTCCTGATGGCATCATCGGACTTCAGCTCATCAATGGCAAGAATGAGTCAGCCCACATCAGTG ACGCCGTGGGCGTGGTGGCACAGGCAGTTCACGAACTCCTAGAGAAGGAGAATATCACTGACCCACCGCG GGGTTGCGTGGGCAACACCAACATCTGGAAGACAGGACCATTGTTCAAGAGGGTGCTGATGTCTTCTAAG TATGCGGACGGAGTGACTGGCCGTGTGGAATTCAATGAGGATGGGGACCGGAAGTTTGCCAACTATAGTA TCATGAACCTGCAGAACCGCAAGCTGGTGCAAGTGGGCATCTACAATGGTACCCATGTCATCCCAAATGA CAGGAAGATCATCTGGCCAGGAGGAGAGACAGAGAAACCTCGAGGATACCAGATGTCCACCAGACTAAAG ATAGTGACAATCCACCAAGAGCCCTTCGTGTACGTCAAGCCCACAATGAGTGATGGGACATGCAAAGAGG AGTTCACAGTCAATGGTGACCCAGTGAAGAAGGTGATCTGTACGGGGCCTAATGACACGTCCCCAGGCAG CCCACGCCACACAGTGCCCCAGTGCTGCTATGGCTTCTGCATAGACCTGCTCATCAAGCTGGCGCGGACC ATGAATTTTACCTATGAGGTGCACCTGGTGGCAGATGGCAAGTTTGGCACACAGGAGCGGGTAAACAACA GCAACAAAAAGGAGTGGAACGGAATGATGGGCGAGCTACTCAGTGGCCAAGCGGACATGATTGTGGCACC ACTGACCATCAACAATGAGCGTGCGCAGTACATAGAGTTCTCCAAGCCCTTCAAGTACCAGGGCCTGACC ATTTTGGTCAAGAAGGAGATTCCCAGGAGCACACTGGACTCATTTATGCAGCCTTTTCAGAGCACACTGT GGTTGCTAGTAGGACTGTCAGTTCATGTGGTGGCTGTGATGCTGTACCTGCTGGACCGCTTCAGTCCCTT TGGCCGATTCAAGGTGAACAGTGAGGAGGAGGAGGAAGATGCACTGACCCTGTCCTCTGCCATGTGGTTT TCCTGGGGCGTCCTGCTCAACTCCGGCATTGGGGAAGGTGCCCCCCGGAGTTTCTCTGCACGTATCCTAG GCATGGTGTGGGCTGGTTTCGCCATGATCATAGTGGCTTCCTACACTGCCAACTTGGCAGCTTTCCTGGT GCTGGATCGGCCTGAGGAGCGCATCACGGGCATCAATGACCCCAGGCTCAGAAACCCCTCAGACAAGTTC ATCTACGCAACTGTAAAGCAGAGCTCCGTGGACATCTACTTCCGGAGGCAGGTGGAGTTGAGTACCATGT ACCGGCACATGGAAAAACACAATTACGAGAGCGCAGCTGAGGCCATCCAGGCTGTGCGGGACAACAAGCT GCACGCCTTTATCTGGGACTCGGCCGTGCTGGAGTTTGAGGCTTCACAGAAGTGCGATCTGGTGACCACG GGTGAGCTGTTCTTCCGCTCAGGCTTTGGCATCGGCATGCGCAAGGACAGCCCCTGGAAGCAGAACGTTT CCCTGTCCATACTCAAGTCCCATGAGAATGGCTTCATGGAAGATCTGGATAAGACATGGGTTCGGTATCA GGAATGCGACTCCCGCAGCAATGCTCCTGCAACCCTCACTTTTGAGAACATGGCAGGGGTCTTCATGCTG GTGGCTGGAGGCATCGTAGCTGGGATTTTCCTCATTTTCATTGAGATCGCCTACAAGCGACACAAGGATG CCCGTAGGAAGCAGATGCAGCTGGCTTTTGCAGCCGTGAACGTGTGGAGGAAGAACCTGCAGGATAGAAA GAGTGGTAGAGCAGAGCCCGACCCTAAAAAGAAAGCCACATTTAGGGCTATCACCTCCACCCTGGCCTCC AGCTTCAAGAGACGTAGGTCCTCCAAAGACACGAGCACCGGGGGTGGACGCGGCGCTTTGCAAAACCAAA AAGACACAGTGCTGCCGCGACGCGCTATTGAGAGGGAGGAGGGCCAGCTGCAGCTGTGTTCCCGTCATAG GGAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217364 representing NM_001270602 Red=Cloning site Green=Tags(s) MSTMHLLTFALLFSCSFARAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPN AIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRT VPPYSHQSSVWFEMMRVYNWNHIILLVSDDHEGRAAQKRLETLLEERESKSKKRNYENLDQLSYDNKRGP KAEKVLQFDPGTKNVTALLMEARELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNAL RYAPDGIIGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSK YADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLK IVTIHQEPFVYVKPTMSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCIDLLIKLART MNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLT ILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWF SWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKF IYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTT GELFFRSGFGIGMRKDSPWKQNVSLSILKSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFML VAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQDRKSGRAEPDPKKKATFRAITSTLAS SFKRRRSSKDTSTGGGRGALQNQKDTVLPRRAIEREEGQLQLCSRHRES TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270602
ORF Size	2877 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270602.1, NP_001257531.1
RefSeq Size	4343 bp
RefSeq ORF	2880 bp
Locus ID	24408
UniProt ID	P35439
MW	108 kDa
Gene Summary	subunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD, Feb 2006]

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