Aak1 (NM_001173450) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1563580
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217367 representing NM_001173450 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAGTTTTTCGACTCCAGGCGGGAGCAGGGGAGCTCTGGCCTGGGTTCGGGCTCCAGCGGAGGAG GGGGCAGCAGCTCAGGCCTGGGCAGTGGCTACATCGGGAGGGTCTTCGGCATCGGGAGGCAGCAAGTCAC CGTGGACGAGGTCTTGGCAGAAGGTGGATTCGCTCTCGTCTTTCTGGTGAGAACAAGCAATGGGGTGAAA TGTGCCTTGAAACGCATGTTTGTCAACAATGAGCATGACCTTCAGGTGTGCAAGAGGGAGATCCAGATCA TGAGGGACCTATCAGGGCACAAGAACATTGTGGGCTACATTGATTCTAGTATCAACAATGTGAGCAGTGG CGACGTATGGGAAGTTCTCATTCTCATGGACTTCTGCAGAGGAGGCCAGGTGGTAAACCTGATGAACCAG CGTCTCCAGACAGGCTTTACAGAGAATGAAGTGCTGCAGATCTTCTGTGACACCTGTGAGGCTGTGGCCC GCCTGCATCAGTGCAAAACTCCCATTATCCACCGGGACCTGAAGGTTGAAAATATCCTCTTGCATGACCG AGGCCACTATGTCTTGTGTGACTTTGGAAGTGCCACCAACAAATTCCAGAATCCACAGGCCGAGGGAGTC AATGCAGTAGAAGATGAGATTAAGAAATACACAACGCTCTCCTATCGAGCCCCAGAAATGGTCAACTTGT ACAGTGGCAAAATCATCACCACGAAGGCAGATATTTGGGCTCTTGGCTGTTTGTTGTATAAATTATGCTA CTTCACTTTGCCATTTGGGGAGAGCCAGGTGGCAATTTGTGATGGAAGCTTCACAATTCCTGATAACTCT CGATATTCACAAGATATGCACTGCCTTATTAGGTATATGTTGGAACCAGACCCTGACAAAAGGCCGGATA TTTACCAGGTTTCTTACTTCTCATTTAAACTACTCAAGAAAGAATGCCCAGTTCCAAATGTACAGAATTC TCCCATTCCTACAAAACTTCCTGAACCAGTGAAAGCCAGTGAGGCAGCTGTAAAAAAGACCCAGCCAAAG GCCAGACTGACAGATCCCATTCCCACCACAGAGACTTCAATTGCACCCCGCCAGAGGCCTAAAGCTGGGC AGACTCAGCCAAACCCCGGAATCCTTCCCATTCAGCCAGCCCTGACTCCTCGTAAGAGGGCCACTGTTCA GCCCCTACCTCAGGCTACAGGACCCAGCAATCAGCCTAGCCTTCTAGCCAGTGTTTCCCAGCCTAAAGCC CAAGCCACGCCCAGTCAACCTCTTCAGTCATCTCAACCCAAGCAGCCACAAGCTCCACCCACCCCACAGC AGACGCCTGCTCCCCAGACCCAGGGTCTGCCCACCCAGGCCCAGGCTACTCCTCAGCACCAGCAGCAGCT CCTCCTCAAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAACAGCCACAGCAGCCAACAGCACCA CCACAGCCATCAGGCACCTTTTACCAGCAGCAGCAGCCCCAGCAGCAGCAGGCTCAGACTCAGCAGTTTC AGGCAGTGCATCCAGCAGCCCAGCAATCAGTCACTGCTCAGTTCCCTGTGGTGTCCCAGGGAGGCTCTCA GCAACAGCTGATGCAGAACTTCTACCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAACAGCTGATGGCT CAGCAGGCCGCCCTGCAGCAGAAGACTGCTGTGGTAGTACCACAGCCTCAGGCACAGCCAGCCACAGCCC CACAGGCAGCTGCTGCCCAGGAGCCTGCGCAGATTCAAGCCCCAGCAAGACAACAGCCAAAAGTTCAGAC CACTCCACCTCCAACCATCCAGGGACAGAAAGTTGGCTCTCTCACTCCTCCATCATCTCCGAAAACCCAA CGTGCTGGGCACAGGCGGATTCTCAGTGATGTAACCCACAGTGCAGTCTTCGGGGTTCCTGCCAGCAAGT CAACCCAGCTGCTCCACGCGGCTGCAGCTGAGGCCAGTCTCAGTAAATCTAAGTCTGCAACCACCACTCC TTCAGGCTCTCCACGGACTTCCCAGCAGAATGTCTCTAATGCTTCCGAAGGCTCCACATGGAATCCTTTT GATGATGACAACTTCTCCAAACTCACGGCTGAAGAGCTGCTGAACAAGGATTTCGCGAAGCTTGGGGAAG GAAAGCTTCCTGAGAAGCTTGGAGGCTCTGCAGAGAGTTTGATCCCAGGTTTCCAGGCAACACAAGGAGA TGCCTTTGCCACTTCCTCCTTTTCTGCTGGAACCGCTGAGAAGAGAAAGGGGGGACAGGCTGTGGATTCT GGCATCCCACTTCTAAGTGTGTCTGATCCTTTCATTCCTCTTCAGGTACCTGATGCTCCAGAAAAACTAA TCGAGGGACTCAAATCTCCTGACACTTCTCTTCTGCTCCCTGACCTCTTGCCTATGACAGATCCTTTTGG CAGCACTTCCGATGCTGTGATTGAAAAAGCTGATGCTGCTGTTGAGAGTCTCATACCTGGGCTGGAGCCA CCTGTTGCCCAGCGCCTCCCATCTCACACGGAATCTGTGACTTCAAACCGCACAGACTCTCTCACGGGGG AAGACTCTCTCCTTGACTGTTCTCTGCTTTCTAACCCTACTGCTGACCTTCTGGACGAGTTTGCCCCCAT AGCACTCTCTGCTTCCACTCATAAAGCTGCAGAAGATAGTAATCTCATCTCAGGGTTTGGTGTCGCTGAG GGCTCCGAAAAGGTGGCAGAAGATGAGTTTGACCCTATTCCTGTACTGATAACCAAAAACACACAAGGTG GGCACTCTAGGAACAGCAGTGGGAGCTCTGAGTCCAGTCTTCCCAACCTAGCCAGGTCTTTGCTGCTGGT GGATCAGCTCATAGACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217367 representing NM_001173450 Red=Cloning site Green=Tags(s) MKKFFDSRREQGSSGLGSGSSGGGGSSSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFALVFLVRTSNGVK CALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQ RLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQAEGV NAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGSFTIPDNS RYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPVPNVQNSPIPTKLPEPVKASEAAVKKTQPK ARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPLPQATGPSNQPSLLASVSQPKA QATPSQPLQSSQPKQPQAPPTPQQTPAPQTQGLPTQAQATPQHQQQLLLKQQQQQQQQQQQQQPQQPTAP PQPSGTFYQQQQPQQQQAQTQQFQAVHPAAQQSVTAQFPVVSQGGSQQQLMQNFYQQQQQQQQQQQQLMA QQAALQQKTAVVVPQPQAQPATAPQAAAAQEPAQIQAPARQQPKVQTTPPPTIQGQKVGSLTPPSSPKTQ RAGHRRILSDVTHSAVFGVPASKSTQLLHAAAAEASLSKSKSATTTPSGSPRTSQQNVSNASEGSTWNPF DDDNFSKLTAEELLNKDFAKLGEGKLPEKLGGSAESLIPGFQATQGDAFATSSFSAGTAEKRKGGQAVDS GIPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADAAVESLIPGLEP PVAQRLPSHTESVTSNRTDSLTGEDSLLDCSLLSNPTADLLDEFAPIALSASTHKAAEDSNLISGFGVAE GSEKVAEDEFDPIPVLITKNTQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001173450
ORF Size	2889 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001173450.1, NP_001166921.1
RefSeq Size	7976 bp
RefSeq ORF	2892 bp
Locus ID	500244
MW	103.9 kDa

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