Hyou1 (NM_001034028) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cab140; Orp150
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217402 representing NM_001034028 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCTACAGTAAGGAGGCAGAGGCCAAGGAGGCTACTCTGTTGGGCCTTGGTGGCTGTCCTCTTGG CAGACCTGTTGGCACTGAGTGACACACTGGCTGTGATGTCTGTGGACCTGGGCAGTGAATCCATGAAGGT GGCCATTGTCAAGCCTGGAGTGCCCATGGAGATTGTATTGAACAAGGAATCTCGGAGGAAAACTCCGGTG ACTGTGACCTTGAAGGAAAACGAAAGGTTTCTAGGTGACAGTGCAGCTGGCATGGCCATCAAGAACCCAA AGGCTACGCTCCGTTATTTCCAGCACCTCCTTGGAAAGCAGGCAGATAACCCTCATGTGGCTCTTTACCG GTCCCGTTTCCCAGAACATGAGCTCAATGTTGACCCACAGAGGCAGACTGTGCGCTTCCAGATCAGTCCG CAGCTGCAGTTCTCTCCCGAGGAGGTGCTGGGCATGGTTCTCAACTACTCCCGTTCCCTGGCTGAAGATT TTGCAGAACAACCTATTAAGGATGCAGTGATCACCGTGCCAGCCTTTTTCAACCAGGCCGAGCGCCGAGC TGTGCTGCAGGCTGCTCGTATGGCTGGCCTCAAGGTGCTGCAGCTCATCAATGACAACACTGCCACAGCC CTCAGCTATGGTGTCTTCCGCCGGAAAGATATCAATTCCACTGCACAGAATATCATGTTCTATGACATGG GCTCGGGCAGCACTGTGTGTACCATCGTGACCTACCAAACGGTGAAGACTAAGGAGGCTGGGACGCAGCC ACAGCTACAGATCCGGGGCGTGGGATTTGACCGCACCCTGGGTGGCCTGGAGATGGAGCTTCGGCTGCGA GAGCACCTGGCTAAGCTCTTCAATGAGCAGCGCAAGGGCCAGAAAGCCAAGGATGTTCGGGAAAACCCCC GAGCCATGGCCAAACTGCTTCGGGAAGCCAATCGGCTTAAAACCGTCCTGAGTGCCAATGCTGATCACAT GGCACAGATTGAAGGCTTGATGGACGATGTGGACTTCAAGGCAAAAGTAACTCGAGTGGAGTTTGAGGAG CTGTGTGCAGATTTGTTTGATCGAGTGCCTGGGCCTGTACAGCAGGCCCTGCAGAGTGCTGAGATGAGCC TGGATCAAATTGAGCAGGTGATCCTGGTGGGTGGGGCCACTCGTGTTCCCAAAGTTCAAGAGGTGCTGCT GAAGGCTGTGGGCAAGGAGGAACTAGGAAAGAACATCAATGCCGATGAAGCAGCTGCCATGGGGGCCGTG TACCAGGCAGCGGCACTGAGCAAAGCCTTCAAAGTGAAGCCATTTGTTGTGCGTGATGCTGTTATTTACC CCATCCTGGTGGAGTTCACAAGGGAGGTGGAGGAGGAGCCTGGGCTTCGAAGCCTGAAGCACAATAAACG TGTGCTCTTCTCCCGAATGGGGCCCTACCCTCAGCGCAAAGTCATCACCTTTAACCGATACAGCCATGAT TTCAACTTTCACATCAACTACGGTGACCTGGGCTTCCTGGGGCCTGAGGATCTTCGGGTATTTGGCTCCC AGAATCTGACCACAGTGAAACTAAAAGGTGTGGGAGAGAGCTTCAAGAAATATCCTGACTATGAGTCCAA AGGCATCAAGGCCCACTTTAACCTAGACGAGAGTGGAGTGCTCAGTTTAGACAGGGTGGAGTCCGTATTC GAGACCCTGGTGGAGGACAGCCCAGAGGAAGAGTCTACTCTTACCAAACTTGGCAACACCATTTCCAGCC TGTTTGGCGGTGGTACCTCATCAGATGCCAAAGAGAATGGTACTGATGCTGTACAGGAGGAGGAGGAGAG CCCTGCTGAGGGGAGCAAGGATGAGCCTGCAGAACAGGGGGAACTCAAGGAGGAAGCTGAAGCCCCAATG GAGGATACCTCCCAGCCTCCACCCTCTGAGCCTAAGGGGGATGCAGCCCGTGAGGGAGAAACACCTGATG AAAAAGAAAGTGGGGACAAGTCTGAGGCCCAGAAGCCCAATGAGAAGGGGCAGGCAGGGCCTGAGGGTGT CCCTCCAGCTCCCGAGGAAGAAAAAAAGCAGAAACCTGCCCGGAAGCAGAAAATGGTGGAGGAGATAGGT GTGGAACTGGCTGTCTTGGACCTGCCAGACTTGCCAGAGGATGAGCTGGCCCATTCCGTGCAGAAACTTG AGGACTTGACCCTGCGAGACCTTGAAAAGCAGGAGAGGGAGAAAGCTGCCAACAGCTTAGAAGCTTTTAT CTTTGAGACCCAGGACAAACTGTACCAACCTGAGTACCAGGAAGTGTCCACTGAGGAACAACGGGAGGAG ATCTCTGGAAAACTCAGTGCCACTTCTACCTGGCTGGAGGATGAGGGATTTGGAGCCACCACTGTGATGT TGAAGGACAAGCTGGCTGAGCTGAGAAAGCTGTGCCAAGGGCTGTTTTTTCGGGTGGAAGAGCGCAGGAA ATGGCCAGAGCGGCTTTCAGCTCTGGATAATCTCCTCAATCACTCCAGCATTTTCCTCAAGGGTGCCCGA CTCATCCCAGAGATGGACCAGATCTTCACTGACGTGGAGATGACAACGTTGGAGAAAGTCATCAATGACA CCTGGACCTGGAAGAATGCAACCCTGGCCGAGCAGGCCAAGCTTCCTGCCACAGAGAAACCCGTGCTGCT TTCAAAAGACATCGAGGCCAAAATGATGGCCCTGGACCGGGAGGTGCAGTATCTACTCAATAAGGCCAAG TTTACTAAACCCCGGCCACGGCCCAAGGACAAGAATGGCACCCGGACAGAGCCTCCCCTCAATGCCAGTG CTGGTGACCAAGAGGAAAAGGTCATTCCACCTACAGGCCAGACTGAAGAGGCGAAGCCCATCTTAGAACC TGACAAAGAGACTGGTACAGAGCCAGCAGACTCTGAGCCTCTGGAATTAGGAGGTCCTGGTGCAGAATCT GAACAGGCAGAGCAGACAGCAGGGCAGAAGCGGCCTTTGAAGAATGATGAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217402 representing NM_001034028 Red=Cloning site Green=Tags(s) MAATVRRQRPRRLLCWALVAVLLADLLALSDTLAVMSVDLGSESMKVAIVKPGVPMEIVLNKESRRKTPV TVTLKENERFLGDSAAGMAIKNPKATLRYFQHLLGKQADNPHVALYRSRFPEHELNVDPQRQTVRFQISP QLQFSPEEVLGMVLNYSRSLAEDFAEQPIKDAVITVPAFFNQAERRAVLQAARMAGLKVLQLINDNTATA LSYGVFRRKDINSTAQNIMFYDMGSGSTVCTIVTYQTVKTKEAGTQPQLQIRGVGFDRTLGGLEMELRLR EHLAKLFNEQRKGQKAKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLMDDVDFKAKVTRVEFEE LCADLFDRVPGPVQQALQSAEMSLDQIEQVILVGGATRVPKVQEVLLKAVGKEELGKNINADEAAAMGAV YQAAALSKAFKVKPFVVRDAVIYPILVEFTREVEEEPGLRSLKHNKRVLFSRMGPYPQRKVITFNRYSHD FNFHINYGDLGFLGPEDLRVFGSQNLTTVKLKGVGESFKKYPDYESKGIKAHFNLDESGVLSLDRVESVF ETLVEDSPEEESTLTKLGNTISSLFGGGTSSDAKENGTDAVQEEEESPAEGSKDEPAEQGELKEEAEAPM EDTSQPPPSEPKGDAAREGETPDEKESGDKSEAQKPNEKGQAGPEGVPPAPEEEKKQKPARKQKMVEEIG VELAVLDLPDLPEDELAHSVQKLEDLTLRDLEKQEREKAANSLEAFIFETQDKLYQPEYQEVSTEEQREE ISGKLSATSTWLEDEGFGATTVMLKDKLAELRKLCQGLFFRVEERRKWPERLSALDNLLNHSSIFLKGAR LIPEMDQIFTDVEMTTLEKVINDTWTWKNATLAEQAKLPATEKPVLLSKDIEAKMMALDREVQYLLNKAK FTKPRPRPKDKNGTRTEPPLNASAGDQEEKVIPPTGQTEEAKPILEPDKETGTEPADSEPLELGGPGAES EQAEQTAGQKRPLKNDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001034028
ORF Size	2994 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001034028.1, NP_001029200.1
RefSeq Size	4346 bp
RefSeq ORF	2997 bp
Locus ID	192235
MW	111.2 kDa
Gene Summary	a stress protein induced by hypoxia [RGD, Feb 2006]

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