Hdac9 (NM_001200045) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1310748; RGD1563092
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217451 representing NM_001200045 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACAGTATGATCAGCTCAGTGGACGTGAAATCAGAGGTTCCTATGGGACTGGAGCCCATCTCACCTT TAGACCTAAGGACAGACCTCAGGATGATGATGCCTGTGGTGGATCCTGTCGTCCGCGAGAAGCAACTGCA GCAAGAGTTACTTCTTATCCAACAGCAGCAGCAAATCCAGAAGCAGCTCCTGATAGCCGAGTTTCAGAAG CAGCACGAGAATTTGACAAGGCAGCATCAAGCACAACTTCAGGAGCACATCAAGTTACAACAGGAACTTC TAGCCATAAAACAACAGCAAGAACTCCTAGAAAAGGAGCAGAAACTGGAGCAGCAGAGGCAAGAACAGGA AGTAGAGAGGCATCGCAGAGAGCAGCAGCTTCCTCCTCTCAGAGGCAAAGATAGAGGACGAGAAAGGGCG GTGGCAAGCACAGAGGTTAAGCAGAAGCTTCAAGAATTCCTATTGAGTAAATCAGCAACGAAAGACACTC CAACCAATGGAAAGAATCATTCCGTGGGCCGCCATCCCAAGCTCTGGTACACGGCTGCCCACCACACATC ACTGGATCAAAGCTCTCCACCCCTCAGTGGAACGTCTCCATCCTACAAATACACATTACCAGGAGCCCAA GATACCAAGGATGATTTCCCCTTGCGAAAAACAGCCTCAGAGCCCAACTTGAAGGTGCGGTCCAGGTTAA AACAGAAAGTGGCAGAGAGGAGAAGCAGCCCCTTACTCAGGCGGAAGGATGGAAATCTTGTCACTTCATT CAAGAAGCGAGTGTTTGAGGTGGCAGAATCCTCGGTCAGTAGCAGCTCTCCAGGGTCAGGTCCCAGTTCA CCAAACAATGGGCCTGCTGGAAATGTTACCGAAAACGAGGCTTCAGTTCTGCCTCCCACGCCTCACCCCG AGCAGCTGGTTCCACAGCAGCGCATTCTGATTCACGAAGATTCCATGAACCTGCTGAGTCTCTACACCTC CCCGTCCCTGCCCAATATCACCCTGGGACTTCCAGCAGTGCCATCCCCACTCAGTGCTTCTAATTCCCTC AAAGACAAGCAGAAGTGTGAGACACAGATGCTCAGACAAGGTGTCCCTCTGCCCGGCCAGTATGGAAGTG GCATTGCAGCATCCTCCAGCCATGCTCATGTAGCAATGGAGGGCAAACCGAACAGCAGTCACCAAGCTCT CCTGCAGCACCTCCTGCTGAAGGAACAAATGAGACAGCAAAAGCTTCTTGTGGCTGGCGGAGTTCCATTA CACCCCCAGTCTCCTTTGGCAACAAAAGAAAGAATTTCACCAGGCATTAGAGGTACCCACAAATTGCCCC GCCACAGACCCCTCAATCGAACTCAGTCTGCACCTTTGCCTCAGAGCACACTGGCTCAGCTGGTCATTCA GCAGCAGCACCAGCAGTTCCTGGAGAAGCAGAAGCAATACCAGCAGCAGATCCACATGAACAAACTGCTA TCGAAATCTATTGAGCAACTGAAGCAACCAGGCAGCCATCTTGAAGAGGCAGAGGAGGAGCTTCAGGGGG ACCAAGCCATGGAAGAGAGAGCAACGTCTAAGGACAGCGGTACCGGGAAGGACAGCAGTGCTTGTGTGGA GGACACACTGGGACAAGTTGGGGCTGTGAAGGTCAAGGAGGAACCCGTGGACAGCGACGAAGATGCTCAG ATGCAGGAAATGGAATGTGGGGAGCAGGCTGCTTTTATGCAACAGACGCCCTTGCTGGAGTCCTCACACA CGCGTGCACTCTCAGTGCACCAGGCTCGGCTGGCTGCAGCTGGCATCGATGGATTAGAGAAACATGGACT CCTCTCCAGGACCCATTCTTCTCCTGCACCCTCCATGTCACCTCACCCAGCCGCAGACTGTCCCCGCCAG CCTGCCTCTGCCACTGGAATTGCCTATGACTGTCTGATGCTGAAACACCAGTGCATCTGTGGCAATTCCA CCACCCACCCTGAGCATGCTGGGCGAATACAGAGCATCTGGTCACGGCTGCAAGAAACCGGGCTGCTAAA TAAATGTGAGCGAATTCAAGGTCGAAAAGCCAGCCTGGAGGAAATTCAGCTTGTTCATTCTGAACATCAC TCACTACTGTATGGCACCAGCCCCCTGGACGGACAGAAGCTGGACCCCAGGACACTCCTAGGTGATGATT CTCGAAAATTCTTTTCCTCGTTACCTTGTGGTGGACTCGGGGTGGACAGTGACACCATTTGGAATGAGCT GCACTCGTCCGGTGCTGCACGCATGGCTGTTGGCTGTGTCATTGAGCTGGCTTCCAAAGTGGCCTCAGGA GAGCTGAAGAATGGGTTTGCTGTTGTGAGGCCCCCAGGCCATCATGCTGAAGAATCTGCAGCCATGGGGT TCTGCTTTTTTAATTCAGTCGCAATTACGGCCAAATACTTGAGAGACCAACTAAATATAAGCAAGATATT GATCGTAGATCTGGATGTTCACCATGGAAACGGTACACAGCAGGCCTTTTATGCTGACCCCAGCATCCTG TACATTTCACTCCATCGCTATGATGAAGGGAACTTTTTCCCTGGCAGTGGCGCCCCGAATGAGGTTGGAG TAGGTCTGGGAGAAGGCTACAATGTAAACATTGCCTGGACAGGCGGCCTTGACCCCCCTATGGGAGATGT TGAGTACCTTGAAGCATTCAGGACTGTCGTGATGCCTGTGGCCAGAGAGTTTGATCCAGACATGGTCCTG GTTTCTGCTGGATTTGATGCATTGGAAGGCCACACCCCTCCCCTAGGGGGTTACAAAGTGACAGCAAAAT GCTTTGGTCATTTGACGAAGCAGCTGATGACATTGGCTAATGGACGTGTGGCATTGGCTCTTGAAGGGGG ACATGATCTGACAGCCATCTGTGATGCCTCGGAGGCCTGCATAAATGCCCTTCTAGGAAATGAGCCCGGG TCTCTTGAGGAAGATGTTCTCCACCAGTCTGTGAATACGAATGCAGCCGCTTCTTTACAGAAGACCATTG AAATCCAAAGCAAGTATTGGAAGTCGATCAAGATGGTGGCTGTTGCAAGGGGCTGTGCTCTGCCTGCTTC TCAGCTGCAAGAGGAGACAGAGACCGTTTCTGCATTAGCCTCCCTGACGGTAGATGTGGAACAGCCATTT GCTCAGGAAGATGGCAGAACTGCTGGTGAGCCCATGGAAGAGGAGCCAGCCTTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217451 representing NM_001200045 Red=Cloning site Green=Tags(s) MHSMISSVDVKSEVPMGLEPISPLDLRTDLRMMMPVVDPVVREKQLQQELLLIQQQQQIQKQLLIAEFQK QHENLTRQHQAQLQEHIKLQQELLAIKQQQELLEKEQKLEQQRQEQEVERHRREQQLPPLRGKDRGRERA VASTEVKQKLQEFLLSKSATKDTPTNGKNHSVGRHPKLWYTAAHHTSLDQSSPPLSGTSPSYKYTLPGAQ DTKDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGNLVTSFKKRVFEVAESSVSSSSPGSGPSS PNNGPAGNVTENEASVLPPTPHPEQLVPQQRILIHEDSMNLLSLYTSPSLPNITLGLPAVPSPLSASNSL KDKQKCETQMLRQGVPLPGQYGSGIAASSSHAHVAMEGKPNSSHQALLQHLLLKEQMRQQKLLVAGGVPL HPQSPLATKERISPGIRGTHKLPRHRPLNRTQSAPLPQSTLAQLVIQQQHQQFLEKQKQYQQQIHMNKLL SKSIEQLKQPGSHLEEAEEELQGDQAMEERATSKDSGTGKDSSACVEDTLGQVGAVKVKEEPVDSDEDAQ MQEMECGEQAAFMQQTPLLESSHTRALSVHQARLAAAGIDGLEKHGLLSRTHSSPAPSMSPHPAADCPRQ PASATGIAYDCLMLKHQCICGNSTTHPEHAGRIQSIWSRLQETGLLNKCERIQGRKASLEEIQLVHSEHH SLLYGTSPLDGQKLDPRTLLGDDSRKFFSSLPCGGLGVDSDTIWNELHSSGAARMAVGCVIELASKVASG ELKNGFAVVRPPGHHAEESAAMGFCFFNSVAITAKYLRDQLNISKILIVDLDVHHGNGTQQAFYADPSIL YISLHRYDEGNFFPGSGAPNEVGVGLGEGYNVNIAWTGGLDPPMGDVEYLEAFRTVVMPVAREFDPDMVL VSAGFDALEGHTPPLGGYKVTAKCFGHLTKQLMTLANGRVALALEGGHDLTAICDASEACINALLGNEPG SLEEDVLHQSVNTNAAASLQKTIEIQSKYWKSIKMVAVARGCALPASQLQEETETVSALASLTVDVEQPF AQEDGRTAGEPMEEEPAL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001200045
ORF Size	3204 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001200045.1, NP_001186974.1
RefSeq Size	3503 bp
RefSeq ORF	3207 bp
Locus ID	687001
MW	117 kDa

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