Cp (NM_001270961) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CERP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217464 representing NM_001270961 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTTTTGCTGCTTAGTGCACTTTTATTTTTGCATAGTTCCTTAGCTTGGACAAGAGAAAAGCATT ATTACATCGGAATTACTGAAGCAGTTTGGGACTATGCTTCTGGCAGTGAAGAAAAGGAACTTATTTCAGT TGACACGGAACAGTCCAATTTCTATCTTCGAAATGGTCCAGATCGTATTGGAAGAAAGTATAAGAAGGCC CTTTATTCTGAGTACACAGATGGCACCTTTACGAAGACTATAGACAAACCAGCCTGGCTAGGGCTTTTAG GCCCTGTCATCAAAGCTGAAGTTGGAGACAAAGTTTCTGTTCACGTAAAGAACTTTGCCTCTAGGCCCTA CACTTTTCATGCTCATGGGGTAACTTACACCAAGGCGAACGAGGGGGCCATCTACCCTGACAACACCACT GATTTTCAAAGAGCCGATGACAAACTGTTTCCTGGACAGCAGTATTTGTACGTGCTGCGTGCCAATGAGC CAAGTCCTGGCGAGGGAGACAGCAATTGTGTGACCAGGATTTACCACTCTCATGTGGATGCTCCAAAAGA TATTGCATCAGGACTCATAGGACCGTTGATACTCTGTAAAAAAGGTTCTCTGCATAAGGAAAAAGAGGAA AATATTGACCAAGAATTCGTACTGATGTTCTCTGTGGTGGATGAAAATCTCAGCTGGTATCTAGAAGATA ACATCAAAACCTTCTGCTCGGAACCAGAGAAAGTCGATAAAGACAATGAAGACTTCCAGGAAAGCAACAG GATGTACTCTATAAATGGATATACATTTGGAAGCCTCCCAGGGCTCTCGATGTGTGCAGAAGACAGAGTG AAGTGGTACCTTTTTGGGATGGGGAATGAAGTTGACGTGCATTCAGCGCTCTTTCATGGTCAAGCCCTGA CCAGCAAGAACTATCATACTGATATAATCAACCTGTTCCCTGCCACTCTAATTGATGTTTCTATGGTGGC CCAGAATCCTGGAGTCTGGATGCTCAGTTGCCAGAACCTGAACCATCTGAAAGCTGGTTTGCAGGCCTTT TTCCAGGTTCGTGACTGCAACAAGCCCTCACCGGACGACGATATCCAAGACAGACATGTGAGACATTATT ACATCGCTGCCGAGGAGACCATTTGGGACTATGCTCCGTCTGGGACAGACACCTTCACTGGAGAGAACTT AACCAGTCTGGGAAGTGATTCAAGGGTATTTTTTGAGCAAGGTGCTACAAGAATTGGTGGCTCTTATAAA AAATTGGTTTATCGTGAGTACACAGATGATTCCTTCACAAACCGGAAGCAAAGAGGCCCTGATGAGGAAC ATCTTGGAATCCTTGGTCCTGTCATTTGGGCAGAAGTAGGAGACATCATTAGAGTCACCTTTCATAACAA AGGACAATTTCCTCTCAGCATTCAGCCAATGGGGGTAAGATTCACCAAGGAAAATGAGGGAACATACTAT GGCCCAGATGGCCGTTCCTCAAAGCAAGCCTCCCATGTGGCTCCCAAAGAAACCTTTACGTATGAATGGA CTGTCCCCAAAGAAATGGGACCCACTTATGCAGATCCTGTGTGCCTATCTAAGATGTATTATTCTGGAGT TGACCTCACCAAAGATATATTTACTGGGCTTATTGGGCCAATGAAAATATGCAAGAAAGGCAGCTTACTT GCAGATGGGAGACAGAAAGATGTAGACAAGGAGTTCTACTTGTTTGCAACAGTGTTTGATGAGAATGAGA GTTTACTCTTGGATGATAATATCAGAATGTTCACAACTGCACCTGAGAATGTGGACAAGGAAGATGAAGA CTTTCAGGAGTCCAACAAGATGCACTCCATGAATGGATTCATGTATGGCAATCTGCCTGGCCTCAATATG TGTCTAGGAGAATCCATCGTGTGGTATTTGTTCAGCGCTGGAAATGAGGCAGACGTGCATGGGATATACT TTTCAGGAAATACCTATCTGTCCAAAGGAGAAAGAAGAGACACTGCAAATCTATTTCCTCATAAAAGTCT CACCCTTCTCATGACACCTGACACAGAAGGGTCTTTTGATGTTGAGTGTCTTACAACAGATCACTACACC GGTGGCATGAAGCAAAAGTACACTGTGAACCAGTGCAAGGGGCAGTTTGAAGATGTCACTCTCTACCAGG GAGAAAGGACCTACTATATTGCAGCAGTGGAGGTGGAATGGGATTATTCACCAAGCAGGGACTGGGAAAT GGAGCTGCACCATTTGCAAGAGCAAAATGTTTCAAATGCATTTTTGGATAAGGAAGAGTTTTTCATAGGC TCAAAGTACAAGAAGGTTGTGTATCGAGAGTTTACTGACAGCACATTCAGAGAACAGGTGAAGAGAAGAG CTGAAGAGGAGCACTTGGGCATCCTCGGTCCACTGATTCATGCAGATGTTGGAGACAAAGTTAAAGTTGT CTTTAAAAATATGGCAAGCAGGCCATATTCAATACATGCCCACGGAGTGAAAACAAAGAGTTCTACAGTT GCTCCAACGTTACCAGGTGAAGTTCGCACTTATATATGGCAAATTCCAGAAAGATCAGGTGCTGGAACGG AGGATTCACCTTGTATCCCATGGGCTTATTACTCAACCGTGGATCGAGTTAAGGATCTCTATAGTGGGCT AATAGGCCCATTGATTGTTTGTCGGAAATCTTATGTGAAAGTATTCAATCCTAAAAAGAAAATGGAGTTT TCCCTTTTGTTTCTAGTATTTGATGAGAATGAATCTTGGTACTTAGATGATAACATCAATACATACTCTG ATCACCCTGAGAAAGTAAACAAAGACAACGAGGAATTCATAGAAAGCAATAAAATGCATGCTATCAATGG GAAAATGTTCGGAAACCTACAAGGTCTCACGATGCACGTGGGAGACGAGGTCAACTGGTATGTGATGGCT ATGGGCAATGAAATAGACCTGCACACTGTACACTTCCACGGCCACAGCTTCCAATACAAGCACAGGGGAA TTCATAGTTCTGATGTCTTTGACCTTTTTCCTGGAACATACCAAACCCTAGAGATGTTTCCCCAAACGCC TGGAACCTGGTTACTCCACTGCCATGTGACTGACCATATTCATGCGGGGATGGTAACTACCTACACTGTT TTACCAAATCAAGCATCGTCTCAGAGCTACAGGATGACCTGGAACATACTCTATACACTGTTAATCAGCA TGACTACTTTATTCCAAATATCTACCAAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217464 representing NM_001270961 Red=Cloning site Green=Tags(s) MKFLLLSALLFLHSSLAWTREKHYYIGITEAVWDYASGSEEKELISVDTEQSNFYLRNGPDRIGRKYKKA LYSEYTDGTFTKTIDKPAWLGLLGPVIKAEVGDKVSVHVKNFASRPYTFHAHGVTYTKANEGAIYPDNTT DFQRADDKLFPGQQYLYVLRANEPSPGEGDSNCVTRIYHSHVDAPKDIASGLIGPLILCKKGSLHKEKEE NIDQEFVLMFSVVDENLSWYLEDNIKTFCSEPEKVDKDNEDFQESNRMYSINGYTFGSLPGLSMCAEDRV KWYLFGMGNEVDVHSALFHGQALTSKNYHTDIINLFPATLIDVSMVAQNPGVWMLSCQNLNHLKAGLQAF FQVRDCNKPSPDDDIQDRHVRHYYIAAEETIWDYAPSGTDTFTGENLTSLGSDSRVFFEQGATRIGGSYK KLVYREYTDDSFTNRKQRGPDEEHLGILGPVIWAEVGDIIRVTFHNKGQFPLSIQPMGVRFTKENEGTYY GPDGRSSKQASHVAPKETFTYEWTVPKEMGPTYADPVCLSKMYYSGVDLTKDIFTGLIGPMKICKKGSLL ADGRQKDVDKEFYLFATVFDENESLLLDDNIRMFTTAPENVDKEDEDFQESNKMHSMNGFMYGNLPGLNM CLGESIVWYLFSAGNEADVHGIYFSGNTYLSKGERRDTANLFPHKSLTLLMTPDTEGSFDVECLTTDHYT GGMKQKYTVNQCKGQFEDVTLYQGERTYYIAAVEVEWDYSPSRDWEMELHHLQEQNVSNAFLDKEEFFIG SKYKKVVYREFTDSTFREQVKRRAEEEHLGILGPLIHADVGDKVKVVFKNMASRPYSIHAHGVKTKSSTV APTLPGEVRTYIWQIPERSGAGTEDSPCIPWAYYSTVDRVKDLYSGLIGPLIVCRKSYVKVFNPKKKMEF SLLFLVFDENESWYLDDNINTYSDHPEKVNKDNEEFIESNKMHAINGKMFGNLQGLTMHVGDEVNWYVMA MGNEIDLHTVHFHGHSFQYKHRGIHSSDVFDLFPGTYQTLEMFPQTPGTWLLHCHVTDHIHAGMVTTYTV LPNQASSQSYRMTWNILYTLLISMTTLFQISTKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270961
ORF Size	3252 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270961.1, NP_001257890.1
RefSeq Size	3607 bp
RefSeq ORF	3255 bp
Locus ID	24268
MW	123.7 kDa
Gene Summary	copper-containing ferroxidase that promotes iron incorporation into transferrin; plays a role in iron metabolism and homeostatis [RGD, Feb 2006]

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