Otud4 (NM_001191700) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1305606
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217478 representing NM_001191700 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCAGCTGTCGGCGCGCCCGACGGCGTGGACCAGGGCGGCGCGGGACCGCTGGAGGATGCGACGC CCATGGACGCCTATCTGCGCAAGCTGGGATTGTATCGGAAATTGGTCGCCAAGGACGGGTCGTGCTTGTT CCGGGCTGTGGCCGAGCAGGTATTACACTCTCAGTCTCGACATGTGGAAGTTAGAATGGCTTGTATTCGC TACCTTCGAGATAACAGAGAGAAATTTGAAGCGTTTATAGAAGGGTCATTTGAAGAATATTTAAAACGTT TGGAAAACCCACAGGAATGGGTAGGACAAGTGGAGATAAGCGCCCTCTCTCTTATGTACAGGAAAGATTT TGTAATATATCAGGAGCCAAATGTTTCTCCTTCGCATGTAACTGAAAATAATTTTCCTGAGAAGGTGTTA CTGTGCTTTTCGAATGGAAATCATTATGACATTGTCTATCCTATAACATACAGAGATAGTTCTGCTGTGT GTCAGTCTCTCCTTTATGAATTGCTGTATGAGAAGGTATTTAAAACTGACGTCAGTAAAATCATGATGGG ACTAGAAACTTCTGAAGTGGCTGATGAAAGCAACAGTGAAATATCAGATTCTGAGGATGACAGTTGCAAG AGTAAAAGCACTGCTGCTACTGATGTGAATGGATTTAAGTCCTCAGGCAGTGAGAACCCTAAGAACCATG GGAACTCAGCTGACCTTCCTTTGTCCAGAAAGGTTCTTAAGTCCCTCAGCCCAGCAGTCTATAGAAATGT GGAGTATGAAATTTGGTTGAAGTCTAAGCAAGCTCAACAAAAACGTGATTATTCCATTGCTGCTGGCTTA CAATATGAAGGAGGAGAGAGATGCCATGTTAGATTGGATCATAATGGGAAGTTATCTAATGCAGACATTC ATGGGGTTCACTCTGAGAATGGACCGGTTTTGTCTGAAGAACTGGGGAAAAAGCAAACACCGAAGAACCT CAAGCCACCTCCCCCAGAAAGCTGGAACACGGTGTCCGGAAAGAAGATGAAAAAACCTAATTCTGGGCAA AATTTCCATTCAGATACGGATTACAGAGGGCCAAAGAATCTAAACAAGCCAATCAAAGCCCCGTCTGCAC TACCTCCTCGCCTGCAGCATCCTCCAGCGGGTGTAAGGCAGCACGCGCTCTCCAGTCATTCTACGGGGCC CCAGTCTCAGAAATCCTCCAGTGAGCATAAGAATCTAAGTCGGGTGCCATCACAGATCACAAGAAAACCT GATCGTGAAAGAGCTGAGGACTTTGATCATGTGAGTCGTGAATCTTACTATTTTGGCCTCTCGCCAGAAG AACGCAGAGAGAAGCAAGCTATTGAAGAGTCTCGGCTACTCTATGAGATTCAGAACCGGGATGAACAGGC TTTTCCGGCTCTTTCTAGTTCATCAGTCAGTCAGTCACCTCAGAATAGCAACGCGTGCGTCCCAAGGAAG TCTTCACATGCGAGGGACAGGAAAGGAAGCATGCGGAGAGTAGATGCAGAGGAACGGAAGGACAAAGACT CTCTACGTGGCCATACTCACATGGATAAAAAACCCGAGCCAAGCACACTGGAGATCAGCGATGATAAATG TACAAGAGTTTCATCACCATCTAAGTCAAAGAAAGAGTGCCCATCTCCTGTAGAACAAAAGCCAGCAGAA CATATACCTTTGTCAAATCCAGCCCCCCTCCTAGTCTCTCCAGAAGTTCATCTCACTCCTGCGGTGCCTT CTTTACCAGCCACTGTGCCAGCCTGGCCAAGTGAACCCACAACTTTCGGACCAACAGGTGTCCCTGCTCA GATTCCCATTTTGTCAGTGACACAGACCACTGGACCTGATGCTGCCGTGTCACAAGCGCATTTAACACCC TCTCCGGTTCCTGTGTCAATTCAGGCAGTTAACCAGCCCTTGATGCCTTTGCCTCAGACAATGAGCCTCT ATCAAGACCCACTCTATCCTGGGTTTCCTTGTAGTGAGAAGGGAGATCGAGCCATTGCACCACCCTATTC ACTGTGTCAGACCGGCGAGGACCTGCCTAAAGATAAGAATATTCTTCGATTCTTCTTCAATCTTGGTGTA AAGGCATATAGTTGCCCTATGTGGGCCCCACACTCTTACCTGTATCCTCTGCACCAGGCGTACATGGCAG CCTGCAGGGTGTACCCAAAGGTCCCTGTTCCTGTGTATCCTCAGAGTACTTGGTTCCAAGAAGCCCCTCC TGCTCAGAGTGAAAGTGACTGCCCCTGTGCTGACGCCCACTACTCTATGCACCCTGAGGCCAGTGTTAAT GGCCAGATGCCACAGGCAGAGATCGGACCACCTGCGTTTTCTTCCCCTCTGGTTATCCCTCCATCTCAGG TGTCTGAAGGTCATGGACAGTTGTCTTACCAACCTGAACTGGAGTCTGAGAGCCCAGGGCAGCTTCTTCA CGCTGAGTATGAAGAGTCACTTAGCGGCAAGAACATGTACCCACAGCAGTCCTTTGGGCCTAATCCATTC TTAGGTCCTGTTCCCATTGCACCTCCTTTCTTCCCTCATGTTTGGTATGGGTATCCTTTTCAGGGATTTG TGGAAAATCCTGTAATGAGGCAAAATATTGTCCTGCCCCCTGATGATAAAGGAGAGTTGGATTTGCCTTT GGAAAATCTAGATCTGTCTAAAGAGTGCGATTCTGTCTCAGCAGTAGACGAGTTCCCAGAAGCCAGAGTT GAAGGCGCACATTCTCTCTCTGAAGCAAGTGTGAGCAGCAAGCATGAAGGCCGGGTGGAGCAGTCGTCCC AGACGCGGAAGGCAGACATAGACTTGTCTTCAGGTTCTTCTGGAGTGGAGGGAAAGGCTCACCCCCCTAC TCAGATTCTAAACCGAGAGAGAGAGGCTGTGTCTGCTGAACCTGAGCCTAAGAGGACCATTCAAAGTCTG AAAGAAAAACCAGAGAAAGTCAAAGACCCCAAGACTGCTGCCGATGTGCTCAGCTCTGGGGCCAACTCTG TGGATAGATTGCAAAGACCAAAAGAAGAGAGTTCAGAAGATGAGAACGAAGTGTCTAATATTTTGAGAAG TGGCAGATCCAAGCAGTTTTATAATCAAACTTATGGAAGCAGGAAGTACAAAAGTGATTGGGGCTCTTCT GGTAGAGGTGGCTATCAACATGTGAGAGGCGAGGAGTCCTGGAAAGGGCAGCCTAACAGAAGCCGGGATG AAGGTTATCAGTACCATCGACATGTTAGAGGACGCCCTTACAGGGGAGATAGGAGGAGGTCAGGGATGGG AGATGGCCACAGGGGACAACACACT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217478 representing NM_001191700 Red=Cloning site Green=Tags(s) MEAAVGAPDGVDQGGAGPLEDATPMDAYLRKLGLYRKLVAKDGSCLFRAVAEQVLHSQSRHVEVRMACIR YLRDNREKFEAFIEGSFEEYLKRLENPQEWVGQVEISALSLMYRKDFVIYQEPNVSPSHVTENNFPEKVL LCFSNGNHYDIVYPITYRDSSAVCQSLLYELLYEKVFKTDVSKIMMGLETSEVADESNSEISDSEDDSCK SKSTAATDVNGFKSSGSENPKNHGNSADLPLSRKVLKSLSPAVYRNVEYEIWLKSKQAQQKRDYSIAAGL QYEGGERCHVRLDHNGKLSNADIHGVHSENGPVLSEELGKKQTPKNLKPPPPESWNTVSGKKMKKPNSGQ NFHSDTDYRGPKNLNKPIKAPSALPPRLQHPPAGVRQHALSSHSTGPQSQKSSSEHKNLSRVPSQITRKP DRERAEDFDHVSRESYYFGLSPEERREKQAIEESRLLYEIQNRDEQAFPALSSSSVSQSPQNSNACVPRK SSHARDRKGSMRRVDAEERKDKDSLRGHTHMDKKPEPSTLEISDDKCTRVSSPSKSKKECPSPVEQKPAE HIPLSNPAPLLVSPEVHLTPAVPSLPATVPAWPSEPTTFGPTGVPAQIPILSVTQTTGPDAAVSQAHLTP SPVPVSIQAVNQPLMPLPQTMSLYQDPLYPGFPCSEKGDRAIAPPYSLCQTGEDLPKDKNILRFFFNLGV KAYSCPMWAPHSYLYPLHQAYMAACRVYPKVPVPVYPQSTWFQEAPPAQSESDCPCADAHYSMHPEASVN GQMPQAEIGPPAFSSPLVIPPSQVSEGHGQLSYQPELESESPGQLLHAEYEESLSGKNMYPQQSFGPNPF LGPVPIAPPFFPHVWYGYPFQGFVENPVMRQNIVLPPDDKGELDLPLENLDLSKECDSVSAVDEFPEARV EGAHSLSEASVSSKHEGRVEQSSQTRKADIDLSSGSSGVEGKAHPPTQILNREREAVSAEPEPKRTIQSL KEKPEKVKDPKTAADVLSSGANSVDRLQRPKEESSEDENEVSNILRSGRSKQFYNQTYGSRKYKSDWGSS GRGGYQHVRGEESWKGQPNRSRDEGYQYHRHVRGRPYRGDRRRSGMGDGHRGQHT SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191700
ORF Size	3315 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191700.1, NP_001178629.1
RefSeq Size	3832 bp
RefSeq ORF	3318 bp
Locus ID	307774
MW	122.7 kDa

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