Lats1 (NM_001134543) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1564085
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217489 representing NM_001134543 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGGGGTGAAAAGCCAGAAGGGTACAGACAGATGAGGCCTAAGACCTTTCCTGCCAGCAACTACT CTGGCAGCAGCCGACAAATGCTGCAGGAGATCCGAGAATCCCTTAGGAATTTATCAAAGCCATCTGATGC CGCAAAGGCCGAGCATAACCTGAACAAGATGTCGACCGAAGATCCCAGGCAGGTGAGAAATCCGCCCAAA TTTGGCACACATCATAAAGCCTTGCAAGAAATTCGAAACTCTCTACTACCTTTCGCAAACGAGACGAGTT CTTCCCGGATCCCTTCAGAAGTTAATCCACAGATGTTTCAGGATTTGCAGGCTGCTGGCTTTGATGAGGA TATGGTTATTCAAGCTCTTCAGAAAACCAATAACAGAAGCATAGAAGCAGCTGTTGAATTCATTAGTAAA ATGAGTTATCAGGATCCTCGTCGAGAGCAGATGGCTGCAGCAGCTGCCAGACCTATCAATGCCACCATGA AACCAGGAAATGTGCAACATTCAATTAGCCGAAAACAAAGCTGGAAAGGTTCTAAAGAGTCTTTAGTTCC TCAGAGACACGGTCCATCTCTAGGAGAAAATGTGGTTTATCGATCTGAAAGTCCCAACTCACAGGCGGAT GTAGGAAGACCTCTGTCTGGATCCGGCATTACGGCATTTGCTCAAGCTCACCCAAGCAATGGACAGAGAG TGAACCCACCACCACCACCTCAAGTTAGGAGTGTTACTCCTCCACCACCTCCACGAGGGCAGACCCCACC TCCACGAGGCACAACTCCCCCTCCCCCCTCGTGGGAGCCTAACTCTCAGACAAAGCGCTACTCTGGGAAC ATGGAATATGTAATCTCCCGAATCTCTCCTGTTCCACCTGGGGCGTGGCAGGAGGGCTACCCTCCACCAC CTCTTAACACTTCCCCCATGAATCCCCCCAACCAGGGACAGAGAGGCATCAGTTCTGTTCCAGTTGGCAG GCAACCAATCATCATGCAAAGTACTAGCAAATTTAACTTTACACCAGGGAGACCTGGAGTTCAGAATGGT GGTGGTCAGACTGATTTTATCATGCACCAAAATGTTGTCCCCACTGGTGCTGTGAATCGGCAACCACCAC CACCATATCCTCTGACCCCAGCTAATGGACAAAGCCCTTCTGCTTTGCAAGCAGGCGGTCCTGCTGCTCC ACCATCATTCTCCAATGGAAATGTTCCTCCAACGATGATGGTGCCGAACAGGAACAGTCATAACATGGAG CTGTATAATATCAATGTCCCTGGACTGCAAACAACTTGGCCCCAGTCGTCTTCTGCCCCTGTGCAATCAT CTCCAAGCAGTGGGCATGAAATTCCTACGTGGCAACCTAACATACCAGTGAGGTCAAATTCTTTTAATAA CCCACTAGGAAGTAGAGCAAGTCACTCTGCTAATTCTCAGCCTTCCGCCACGACAGTTACTGCCATCACA CCAGCTCCTATTCAGCAGCCCGTGAAAAGCATGCGCGTTCTGAAACCAGAGCTGCAGACTGCCTTAGCCC CAACCCATCCTTCTTGGATGCCACAGCCAGTTCAGACCGTTCAGCCTAGCCCTTTTTCTGAGGGTGCAGC TTCAAACGTGCCAGTCATCCCACCTGTGGCGGAAGCTCCAAGCTATCAGGGCCCACCACCACCTTATCCA AAACATCTGCTACACCAAACCCCATCTGTTCCTCCATATGAGTCAGCGAGTAAACCCTGCAAAGACGATC AGCCTAGCTCAGCCAAGGAAGAGGATAGTGAAAAGAGTTTGGACAGTGTTGACTCTGGGGATAAAGAGAA GAAACAGATTACAACTTCACCTATCACTGTTCGGAAAAACAAGAAAGACGAAGAGCGAAGAGAGTCTCGC ATTCAGAGTTACTCCCCGCAGGCCTTTAAATTCTTCATGGAGCAACACGTAGAGAACGTCCTGAAGTCTC ATCAGCAGCGTCTGCATCGCAAGAAGCAACTAGAGAATGAAATGATGCGGGTTGGATTATCTCAAGATGC CCAGGATCAAATGAGAAAGATGCTTTGCCAGAAAGAGTCTAACTATATTCGTCTTAAAAGGGCTAAAATG GACAAGTCTATGTTTGTAAAGATAAAGACATTAGGAATAGGAGCATTTGGTGAAGTCTGTCTAGCAAGAA AAGTCGATACGAAAGCTTTGTATGCAACAAAAACTCTACGAAAGAAAGACGTTCTGCTCCGAAATCAGGT GGCTCATGTGAAAGCTGAGCGAGATATCCTAGCAGAAGCTGACAACGAGTGGGTGGTCCGCTTGTACTAC TCATTCCAGGACAAGGACAACTTGTACTTTGTGATGGACTACATTCCTGGGGGTGATATGATGAGCCTGT TAATTAGAATGGGCATCTTTCCCGAAAACCTGGCACGATTCTACATAGCAGAACTTACCTGTGCAGTTGA AAGTGTTCATAAAATGGGTTTTATTCATAGAGACATTAAACCTGATAACATTTTGATTGACCGAGATGGA CATATTAAATTGACTGACTTTGGCTTGTGCACTGGCTTCAGATGGACACATGACTCCAAGTACTACCAGA GTGGGGATCACCCACGGCAAGATAGCATGGATTTCAGTAGCGAATGGGGAGATCCTTCCAACTGTCGGTG TGGGGACAGACTGAAGCCACTAGAGCGGAGAGCTGCTCGCCAGCACCAGCGATGTCTAGCTCACTCTCTG GTTGGGACTCCCAACTATATAGCACCTGAAGTGCTACTACGGACAGGATATACACAGCTGTGTGATTGGT GGAGTGTTGGTGTTATTCTTTTTGAAATGTTGGTGGGACAACCTCCTTTCTTGGCACAAACACCATTAGA AACCCAAATGAAGGTTATCAACTGGCAAACTTCTCTTCACATCCCACCTCAAGCCAAGCTCAGTCCTGAA GCCTCTGACCTCATTATCAAACTTTGCCGAGGACCAGAAGACCGTCTAGGCAAGAACGGTGCTGATGAAA TAAAGGCTCATCCATTTTTTAAGACCATTGATTTCTCTAGTGATCTGAGACAGCAGTCTGCTTCATACAT CCCCAAAATCACGCACCCAACAGACACGTCCAATTTTGACCCTGTTGACCCTGATAAGTTGTGGAGCGAT GGTAACGAGGAAGAAAATATCAACGACACTCTGAACGGATGGTATAAAAACGGAAAGCACCCTGAGCATG CTTTCTATGAGTTTACCTTTCGGAGGTTCTTTGATGACAATGGCTACCCATATAATTATCCAAAGCCCAT CGAATATGAATACGTTAGTTCCCAGGGCTCAGAGCAGCAGTCCGATGAAGACGATCAACACACAGGCTCA GATGTAAACAACCGTGATCTAGTGTATGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217489 representing NM_001134543 Red=Cloning site Green=Tags(s) MKRGEKPEGYRQMRPKTFPASNYSGSSRQMLQEIRESLRNLSKPSDAAKAEHNLNKMSTEDPRQVRNPPK FGTHHKALQEIRNSLLPFANETSSSRIPSEVNPQMFQDLQAAGFDEDMVIQALQKTNNRSIEAAVEFISK MSYQDPRREQMAAAAARPINATMKPGNVQHSISRKQSWKGSKESLVPQRHGPSLGENVVYRSESPNSQAD VGRPLSGSGITAFAQAHPSNGQRVNPPPPPQVRSVTPPPPPRGQTPPPRGTTPPPPSWEPNSQTKRYSGN MEYVISRISPVPPGAWQEGYPPPPLNTSPMNPPNQGQRGISSVPVGRQPIIMQSTSKFNFTPGRPGVQNG GGQTDFIMHQNVVPTGAVNRQPPPPYPLTPANGQSPSALQAGGPAAPPSFSNGNVPPTMMVPNRNSHNME LYNINVPGLQTTWPQSSSAPVQSSPSSGHEIPTWQPNIPVRSNSFNNPLGSRASHSANSQPSATTVTAIT PAPIQQPVKSMRVLKPELQTALAPTHPSWMPQPVQTVQPSPFSEGAASNVPVIPPVAEAPSYQGPPPPYP KHLLHQTPSVPPYESASKPCKDDQPSSAKEEDSEKSLDSVDSGDKEKKQITTSPITVRKNKKDEERRESR IQSYSPQAFKFFMEQHVENVLKSHQQRLHRKKQLENEMMRVGLSQDAQDQMRKMLCQKESNYIRLKRAKM DKSMFVKIKTLGIGAFGEVCLARKVDTKALYATKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYY SFQDKDNLYFVMDYIPGGDMMSLLIRMGIFPENLARFYIAELTCAVESVHKMGFIHRDIKPDNILIDRDG HIKLTDFGLCTGFRWTHDSKYYQSGDHPRQDSMDFSSEWGDPSNCRCGDRLKPLERRAARQHQRCLAHSL VGTPNYIAPEVLLRTGYTQLCDWWSVGVILFEMLVGQPPFLAQTPLETQMKVINWQTSLHIPPQAKLSPE ASDLIIKLCRGPEDRLGKNGADEIKAHPFFKTIDFSSDLRQQSASYIPKITHPTDTSNFDPVDPDKLWSD GNEEENINDTLNGWYKNGKHPEHAFYEFTFRRFFDDNGYPYNYPKPIEYEYVSSQGSEQQSDEDDQHTGS DVNNRDLVYV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001134543
ORF Size	3390 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001134543.2, NP_001128015.2
RefSeq Size	7087 bp
RefSeq ORF	3393 bp
Locus ID	308265
MW	126.4 kDa

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