Ap4e1 (NM_001191742) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1562199
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217490 representing NM_001191742 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGACATGGTGGAGAGGACGCTGACCGCACTGCCGGGGCTCTTCTTGCAGAACCAGCTGGGAGGGC CTGCGGCCTCCAGAGCACCCTTCTTCTCCAGGCTGGGCGGCCTCATCCGTGGCGTCACTGCGCTCTCATC CAAGCACGAAGAAGAGAAGTTAATCCAGCAGGAACTGAGTAGTCTGAAGGCGACTGTTTCTGCTCCTACT ACAACACTGAAAATGATGAAGGAATGTATGGTGAGACTTATATACTGTGAGATGCTTGGATACGATGCTT CTTTTGGTTATATACATGCAATCAAGTTGGCCCAACAAGGAAACCTCTTAGAAAAAAGAGTGGGTTATTT GGCTGTTTCTCTGTTTCTACATGAAAGTCATGAACTTTTGCTTCTCCTTGTGAATACAGTGGTAAAGGAC CTGCAGAGCACTAATCTGGTAGAAGTGTGTATGGCACTCACTGTCGTCAGCCAGATTTTCCCTCGAGAAA TGATTCCAGCTGTCCTTCCACTAATAGAAGAGAAACTCCAACATTCTAAGGAGATTATACGAAGAAAAGC AGTTCTGGCATTATACAAATTTTATCTCATTGCCCCTAATCAAGTACAGCATATCCATACTAAGTTTCGG AAAGCACTCTGTGACAGAGATGTTGGGGTCATGGCTGCCTCTTTGCATATATACCTTAGGATGATTAAGG AAAATTCTTCTGGATATAAAGACTTGACTGAGAGTTTTGTAACAATTTTAAAGCAGGTGGTGGGGGGAAA GCTCCCTGTAGAGTTCAGTTACCACAGTGTGCCAGCGCCATGGTTACAGATTCAGCTCTTGAGAATACTG GGACTGCTGGGAAAAGACGATGAAAGGACAAGTGAACTAATGTATGATGTTCTTGATGAATCCTTACGAA GAGCCGAGTTAAATCACAATGTCACTTACGCTATTCTGTTTGAGTGTGTACACACAATCTATTCTATTTA TCCTAAATCAGAATTACTTGAGAAGGCTGCCAAGTGCATTGGAAAATTTGTTCTGTCACCTAAAATAAAC CTCAAATATTTAGGACTGAAGGCTCTTACCTATGTTATCCAGCAGGATCCTTCTCTGGCTCTTCAACACC AGATAACCATAATTGAATGCCTAGACCACCCTGATCCCATTATTAAAAGAGAGACTCTGGAACTTCTTTA CAGAATTACAAATGCACAGAATGTAGTAGTTATTGTACAGAAAATGCTTGAATATTTACATCAGAGCAAA GAAGAGTACATCATCATCAACTTAGTCGGCAAAATAGCTGAATTGGCTGAGAAATATGCTCCTGACAACG TGTGGTTCATTCAGACGATGAATGCTGTGTTTTCAGTGGGAGGAGATGTAATGCACCCAGACATTCTCAG TAACTTTCTGAGACTGCTAGCAGAAGGTTTTGATGATGAAACAGAAGATCAACAGTTAAGACTCTATGCA GTTCAGTCTTACCTCACCTTACTAGACATGGAAAACACTTTCTATCCACAGAGATTTCTTCAAGTTATGA GTTGGGTATTAGGAGAGTATTCCTACCTCTTAGATAGAGAAAGCCCCGAGGCAGTTATGACCAGGCTCTA CAAGTTACTGAGGAGTGACTCCATTTCTTCAGAAACGAAAGCATGGTTGTTTGCTGCAGTAACCAAGTTA ACACCTCAAGCTCATTCTTCTCCCATAGTTGAGAACTTAATCCAAGAATTCACTGTATCCTTGAATACTT GTCTGAGACAACATGCATTTGAATTGAAACATCTACATGAAAATGAAGAGCTTATGAAGAGCTTGCTTCA GAGTGCTCAGAATTGTGACGACATCATGGCAGATGCTTCTTTATCTTTTCTGGATGGTTTTGTGGCTGAA GGACTCAGTCAGGGAGCAGCACCTTATAAGCCCCGTCACCAGCGCCAAGAGGAGCAGCTCTCTCAGGAAA AAGTTCTCAATTTTGAACCATATGGACTATCATTTTCTTCATCTGGCTTCACTGGACGACAGTCTCCTGC TGGCGTTTCTCTGGGCTCAGATATATCTGGAAACAGTGCTGAAACAGGGCTGAAAGAGGCAGGTAGCCTG AAGATGGACGGCATAAAGAAATTATGGGGTAAAGAAGGTTATCTCCCCAAGAAGGAGAGTGGAGCTGCTG ATAAACTGGAAGCCGCACATGCTCCTGCAGAGAGTGCAATGATGGAGAATGTAGACCAAGCCATGACTAG GAAGGACCAAGCTCAAGGCCACACCCCTTCTACAGAGGAGAAAGAAAAGCAGCTGTTGGCATCATCGCTG TTTGTTGGGCTAGGACCAGAAAATACGATCAATTTGTTGGGAAAAGCAGATGTTGTGTCTTACAAGTTCA GGAGGAAGTCAAAACCCAAGGTAGCCCAAAGTGACAAGATGCCCAGCGCTCATGCCGCTCCCTCCTCCAC CCTTAGCCCTGTGTCCGATGTGGCTGGGGGGGATGAGGACAGTCTGAGCGCTGCGGATAGAGGAGACGGG GAGCTTGGTTCAGAGCTGTTGGGTTCTGAGTCTGTCTTGGAGCTGCCCTCAGTTGAAAACCTCTCGAGTG TCAGCTTGCCCGTGTCTTCTTTGTTTGCGGGTAACGACATGGAAGTATTTCATCCCCCTTCATCCTCTGC AACTTCAGCCGTCAAGGAAGTCAGTTTGGCTGCTCTGTTGGAAGAAACTCCTGAATGCAGGCGTTCAGCT CTCGTGGAAATCTGTAGTAATGAAACTGTGGCTGTGTCTTCTTACAAAGTCTGGAAGGATGAGTGTTTAT TGCTGGTGTGCTCAGTCACTAGTAAGACTGATTCAGAGTTCACAGATGCTCAATTAGAAATTTTTCCTTT GGAAAATTTCAAGATCATTGAGCAGCCTGGATGTCCTTCACCTGTGATGGAAGCAGAAAGCACCAAATCC TTCCAATATGGCGTGCAGATGGAAAGCCCTTGTGTAGAGGGGACTCTCTCTGGTTTTATAAAGTATCAAA TGATGGATACACATCCTGTTCAACTGGAATTTTCCATGAACTTATCACTACTAGATTTTATTAGGCCATT AAAGATCTCAACTGAAGACTTTGGCAAACTCTGGTTGTCTTTTGCAAATGATGTGAAGCAAAATATAAAA ATATCAGAGCCTCGAGTTGCACTGGCTTCTGTCCTGACAGAGCTGCAGCAGAAACTCAGACTTTGCGTGG TTGACGTCATAGGCAGTGAAGGTCTGCTGGCCTGTAAGCTGCTTCCGTCCACCCCCTGTATCCTGCACTG CCGAGTACACGCTGACGTGGTAGCCCTGTGGTTCCGATCCTCTAGCTCTGTTCTTTCAGACTGTTTATCT TGTCACTGTCAGAAAGTGATGCAGACATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217490 representing NM_001191742 Red=Cloning site Green=Tags(s) MSDMVERTLTALPGLFLQNQLGGPAASRAPFFSRLGGLIRGVTALSSKHEEEKLIQQELSSLKATVSAPT TTLKMMKECMVRLIYCEMLGYDASFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVKD LQSTNLVEVCMALTVVSQIFPREMIPAVLPLIEEKLQHSKEIIRRKAVLALYKFYLIAPNQVQHIHTKFR KALCDRDVGVMAASLHIYLRMIKENSSGYKDLTESFVTILKQVVGGKLPVEFSYHSVPAPWLQIQLLRIL GLLGKDDERTSELMYDVLDESLRRAELNHNVTYAILFECVHTIYSIYPKSELLEKAAKCIGKFVLSPKIN LKYLGLKALTYVIQQDPSLALQHQITIIECLDHPDPIIKRETLELLYRITNAQNVVVIVQKMLEYLHQSK EEYIIINLVGKIAELAEKYAPDNVWFIQTMNAVFSVGGDVMHPDILSNFLRLLAEGFDDETEDQQLRLYA VQSYLTLLDMENTFYPQRFLQVMSWVLGEYSYLLDRESPEAVMTRLYKLLRSDSISSETKAWLFAAVTKL TPQAHSSPIVENLIQEFTVSLNTCLRQHAFELKHLHENEELMKSLLQSAQNCDDIMADASLSFLDGFVAE GLSQGAAPYKPRHQRQEEQLSQEKVLNFEPYGLSFSSSGFTGRQSPAGVSLGSDISGNSAETGLKEAGSL KMDGIKKLWGKEGYLPKKESGAADKLEAAHAPAESAMMENVDQAMTRKDQAQGHTPSTEEKEKQLLASSL FVGLGPENTINLLGKADVVSYKFRRKSKPKVAQSDKMPSAHAAPSSTLSPVSDVAGGDEDSLSAADRGDG ELGSELLGSESVLELPSVENLSSVSLPVSSLFAGNDMEVFHPPSSSATSAVKEVSLAALLEETPECRRSA LVEICSNETVAVSSYKVWKDECLLLVCSVTSKTDSEFTDAQLEIFPLENFKIIEQPGCPSPVMEAESTKS FQYGVQMESPCVEGTLSGFIKYQMMDTHPVQLEFSMNLSLLDFIRPLKISTEDFGKLWLSFANDVKQNIK ISEPRVALASVLTELQQKLRLCVVDVIGSEGLLACKLLPSTPCILHCRVHADVVALWFRSSSSVLSDCLS CHCQKVMQTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191742
ORF Size	3390 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191742.1, NP_001178671.1
RefSeq Size	3448 bp
RefSeq ORF	3393 bp
Locus ID	311404
MW	125.5 kDa

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