Grin3a (NM_138546) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	chi-1; GluN3A; NR3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217494 representing NM_138546 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGACTGAGTTTGTGGTGGCTGCTGAGCAGGGTCTGTCTGCTGCTGCCGCCGCCCTGCGCACTGG TGCTGGCCGGGGTGCCCAGCTCCTCCTCGCACCCGCAACCCTGCCAGATCCTCAAGCGCATCGGACACGC GGTGAGGGTGGGCGCGGTGCACTTGCAACCCTGGACCACGGCCCCACGCGCAGCCAGTCGCGCTCAGGAA GGCGGCAGGGCGGGTGCCCAGAGGGATGATCCAGAGTCCGGGACGTGGCGGCCACCGGCGCCCTCGCAAG GCGCACGCTGGTTGGGGAGCGCCCTGCATGGCCGGGGTCCACCCGGCTCCCGAAAGCTCGGGGAGGGCGC GGGGGCCGAGACCCTGTGGCCGCGGGATGCCCTACTGTTCGCTGTGGAAAACTTGAACCGTGTGGAAGGG CTCCTACCCTACAACCTGTCTTTGGAAGTAGTGATGGCCATTGAGGCGGGCCTGGGCGATCTGCCGCTTA TGCCCTTCTCTTCCCCAAGCTCACCGTGGAGCAGTGACCCTTTCTCCTTTCTGCAGAGCGTGTGCCACAC CGTAGTGGTACAAGGGGTGTCGGCGCTGCTGGCCTTCCCCCAGAGCCAGGGCGAAATGATGGAGCTGGAC TTGGTCAGCTCTGTCCTGCACATCCCAGTGCTCAGCATAGTGCGCCACGAGTTTCCGCGGGAGAGTCAGA ATCCCCTGCACCTACAGCTGAGTTTAGAAAACTCACTAAGTTCTGATGCTGATGTCACTGTCTCAATCCT GACCATGAACAACTGGTACAATTTTAGCTTGTTGCTCTGCCAGGAAGACTGGAATATCACGGACTTCCTC CTCCTTACGGAGAATAACTCCAAGTTCCACCTCGAGTCTGTTATCAACATCACTGCTAATCTGTCCTCCA CAAAGGACCTTCTAAGTTTCCTGCAGGTCCAGATGGACAACATTAGGAACAGCACACCCACAATGGTGAT GTTTGGCTGTGACATGGACAGTATCCGGCAGATATTTGAAATGTCCACACAGTTTGGGCTATCACCTCCT GAACTTCACTGGGTTTTAGGAGACTCCCAGAATGTGGAGGAACTGAGGACAGAAGGCCTGCCCTTAGGGC TCATTGCTCATGGAAAAACCACACAATCTGTCTTTGAGTACTACGTTCAGGATGCCATGGAGTTGGTTGC AAGAGCTGTAGCCACAGCCACCATGATCCAGCCAGAGCTTGCTCTCCTTCCCAGCACAATGAACTGCATG GATGTGAAAACCACAAATCTCACTTCTGGACAGTATTTATCAAGGTTTTTAGCCAACACCACTTTCAGAG GTCTCAGTGGTTCCATCAAAGTAAAGGGCTCCACCATCATCAGCTCAGAGAACAACTTTTTCATCTGGAA CTTGCAGCACGACCCTATGGGAAAGCCAATGTGGACTCGCCTGGGCAGCTGGCAAGGAGGAAGGATAGTC ATGGACTCTGGAATATGGCCAGAGCAGGCCCAGAGGCACAAAACCCACTTCCAGCACCCAAACAAGTTAC ACTTGAGAGTGGTGACATTGATTGAACACCCATTTGTTTTCACGAGAGAAGTAGACGATGAAGGCTTATG CCCTGCTGGACAGCTCTGTCTAGACCCTATGACTAACGACTCTTCCATGCTGGATCGTCTGTTTAGCAGC CTGCATAGCAGTAATGACACAGTGCCAATCAAGTTCAAGAAGTGCTGTTATGGGTACTGCATCGATCTCC TGGAGCAGCTAGCAGAAGACATGAACTTTGACTTTGACCTCTACATTGTAGGGGATGGAAAGTACGGAGC TTGGAAAAATGGTCACTGGACTGGGCTGGTTGGTGATCTCCTGAGTGGGACAGCCAACATGGCGGTCACT TCTTTCAGCATCAATACCGCACGAAGCCAGGTGATAGATTTCACCAGCCCTTTCTTCTCAACCAGTTTGG GGATCTTAGTGAGGACTCGAGACACAGCAGCTCCAATTGGAGCCTTCATGTGGCCACTCCACTGGACCAT GTGGCTGGGAATTTTCGTGGCTCTCCATATCACTGCCATTTTTCTCACTCTGTATGAATGGAAGAGCCCC TTTGGTATGACCCCTAAGGGGAGGAACAGAAACAAAGTCTTCTCCTTCTCCTCAGCTTTGAATGTCTGCT ATGCCCTTCTGTTTGGCAGAACAGCAGCCATCAAACCCCCAAAATGCTGGACTGGAAGGTTTCTGATGAA TCTTTGGGCCATTTTCTGTATGTTTTGCCTTTCTACATACACAGCGAACTTGGCTGCTGTCATGGTAGGT GAGAAGATCTATGAAGAGCTTTCTGGAATTCATGACCCTAAGCTTCATCATCCTTCTCAAGGCTTCCGCT TTGGAACTGTCCGGGAAAGCAGTGCTGAAGACTATGTGCGCCAGAGCTTCCCAGAGATGCATGAGTACAT GAGAAGGTACAACGTGCCAGCCACCCCTGATGGAGTGCAGTATCTGAAGAATGATCCAGAGAAACTAGAC GCCTTCATCATGGACAAAGCCCTTCTGGATTATGAAGTGTCAATAGATGCTGACTGCAAGCTTCTGACCG TAGGAAAGCCATTTGCCATCGAAGGATATGGCATTGGTCTCCCTCCAAACTCTCCATTGACCTCTAATAT ATCTGAGCTCATCAGTCAGTACAAGTCTCACGGGTTTATGGATGTGCTCCATGACAAGTGGTACAAGGTG GTTCCCTGCGGAAAGAGAAGCTTTGCCGTCACTGAGACTTTGCAAATGGGCATCAAGCACTTCTCTGGAC TCTTCGTGCTGTTGTGCATAGGATTTGGTCTCTCCATCCTGACCACCATTGGTGAACACATAGTGCACAG ACTGCTGTTACCACGCATCAAAAACAAATCCAAGCTGCAGTACTGGCTGCACACGAGTCAGAGGTTTCAC AGAGCATTAAACACGTCATTCGTAGAAGAAAAGCAGCCACGTTCCAAGACAAAACGTGTGGAGAAGAGCA GATGGAGAAGATGGACCTGCAAGACAGAAGGGGACTCTGAATTGTCTCTGTTTCCTAGGTCCAACCTGGG ACCCCAGCAGCTCATGGTATGGAATACTTCCAATCTGAGTCATGACAACCAACGAAAATACATCTTTAAT GACGAGGAAGGACAAAACCAGCTGGGTACCCAGGCCCACCAGGACATCCCTCTCCCTCAGAGGAGAAGAG AGCTCCCTGCCTCACTGACCACCAATGGGAAAGCAGACTCCCTCAATGTAACTCGGAGCTCCGTGATTCA GGAACTCTCTGAGTTGGAGAAGCAGATCCAAGTGATCCGCCAGGAGCTGCAGTTGGCTGTAAGCAGGAAG ACAGAGCTGGAGGAGTATCAAAAGACAAATCGGACTTGTGAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217494 representing NM_138546 Red=Cloning site Green=Tags(s) MRRLSLWWLLSRVCLLLPPPCALVLAGVPSSSSHPQPCQILKRIGHAVRVGAVHLQPWTTAPRAASRAQE GGRAGAQRDDPESGTWRPPAPSQGARWLGSALHGRGPPGSRKLGEGAGAETLWPRDALLFAVENLNRVEG LLPYNLSLEVVMAIEAGLGDLPLMPFSSPSSPWSSDPFSFLQSVCHTVVVQGVSALLAFPQSQGEMMELD LVSSVLHIPVLSIVRHEFPRESQNPLHLQLSLENSLSSDADVTVSILTMNNWYNFSLLLCQEDWNITDFL LLTENNSKFHLESVINITANLSSTKDLLSFLQVQMDNIRNSTPTMVMFGCDMDSIRQIFEMSTQFGLSPP ELHWVLGDSQNVEELRTEGLPLGLIAHGKTTQSVFEYYVQDAMELVARAVATATMIQPELALLPSTMNCM DVKTTNLTSGQYLSRFLANTTFRGLSGSIKVKGSTIISSENNFFIWNLQHDPMGKPMWTRLGSWQGGRIV MDSGIWPEQAQRHKTHFQHPNKLHLRVVTLIEHPFVFTREVDDEGLCPAGQLCLDPMTNDSSMLDRLFSS LHSSNDTVPIKFKKCCYGYCIDLLEQLAEDMNFDFDLYIVGDGKYGAWKNGHWTGLVGDLLSGTANMAVT SFSINTARSQVIDFTSPFFSTSLGILVRTRDTAAPIGAFMWPLHWTMWLGIFVALHITAIFLTLYEWKSP FGMTPKGRNRNKVFSFSSALNVCYALLFGRTAAIKPPKCWTGRFLMNLWAIFCMFCLSTYTANLAAVMVG EKIYEELSGIHDPKLHHPSQGFRFGTVRESSAEDYVRQSFPEMHEYMRRYNVPATPDGVQYLKNDPEKLD AFIMDKALLDYEVSIDADCKLLTVGKPFAIEGYGIGLPPNSPLTSNISELISQYKSHGFMDVLHDKWYKV VPCGKRSFAVTETLQMGIKHFSGLFVLLCIGFGLSILTTIGEHIVHRLLLPRIKNKSKLQYWLHTSQRFH RALNTSFVEEKQPRSKTKRVEKSRWRRWTCKTEGDSELSLFPRSNLGPQQLMVWNTSNLSHDNQRKYIFN DEEGQNQLGTQAHQDIPLPQRRRELPASLTTNGKADSLNVTRSSVIQELSELEKQIQVIRQELQLAVSRK TELEEYQKTNRTCES TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_138546
ORF Size	3405 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_138546.2, NP_612555.1
RefSeq Size	4386 bp
RefSeq ORF	3408 bp
Locus ID	191573
UniProt ID	Q9R1M7
MW	127.6 kDa
Gene Summary	glutamate receptor subunit involved in neuronal signaling [RGD, Feb 2006]

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