Dusp27 (NM_001191929) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1560598
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217497 representing NM_001191929 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACCAGTGGAGACACAGAGGAGGAGCAGGTAGTTCCAAACGAGGAGGATGAAGCCGACGTGAGAG CTGTACAGGCCCACTACCTCCGGAGTCCCTCCCCAAGCCAGTACTCAGTGGTCTCAGACGCAGAAACTGA AAGTATTTTCATGGAGCCCATCCATCTCTCCTCGGCCGTAGCTGCCAAGCAGATCATCAACGAAGAACTC AAGCCTCGGGGTCTCAGAACAGACACGGAGTGCCCAGGCATGCTGGAGTCTGCTGAACAGCTGCTGGTGG AAGACCTGTACAACCGTGTCCGGGAGAAGATGGATGACAAAAGCCTGTTCAACACCCCCTGTGTGCTGGA CCTGCAGCGGGCACTGATCCAGGACCGGCAAGAGGCCCCTCGGAATGAGGTGGACGAGGTCTGGCCCAAC GTCTTCATAGCTGAGAAGAGTGTGGCTGTGAACAAGGGGCGACTGAAAAGGCTGGGAATCACCCACATCC TGAACGCTGCCCATGGCACTGGCGTTTACACTGGTCCCGAATTCTACACTGGCCTGGAGATCCAGTACCT GGGGGTGGAAGTGGATGATTTCCCAGAGGTGGACATCTCCCAGCATTTCCGGAAGGCAGCTGAGTTTCTT GATGAAGCCCTGCTGACCTATAGAGGGAAAGTCCTGGTCAGCAGTGAGATGGGTATCAGCAGGTCAGCAG TGCTGGTGGTCGCCTACCTGATGATCTTCCACAACATGGCCATCCTGGAAGCCTTGATGACTGTCCGCCG AAAGCGGGCTATCTACCCCAACGACGGCTTCCTCAAACAGCTGCGGGAGCTCAACGAGAAACTGATGGAG GAGAGGGAAGAAGAGGATGATGAAGAGGAGCCTGAGGAGGATGCCGGGAGCACACTGGGTGCTAGAGTAA ATTCCTTGATGGTGGAAGAAGAAGATGATGCCACCAGCCACCTGAGTGGCTCCTCCTTGGGGAAGGCCAG CCAGGTCTCCCAGCCAGTCACCCTCATTGATGAAGAGGAGGAAGAGAGGCTGTATGAGGAGTGGAGGAAG GGGCAGGGCTTCCCCAAGGCTGAGGCTTCTCAGGGTAGAAAGGGTAGCTGTTCTGCGTCCTCCGAGCAAG ACGGGGACGACTGTGAGGATGGGGCTGTGGAGAGGATAATCCAGGAGTGGCAGAGCCGAAATGAGAGGTA CCAAGCCAAAGGGCACAAGCAGTGGAATCGGGAGGAAGACGAGGAGGAAGAGAACTCCTACACCAGCAGA AGGCGCAGACACACCCTAAGCGAGAGCAGCGCCTCTGAGAGCGTGAGCAGCCATGACATCCGGGTCCTGA AGCAGCAACTGAAGAGGACCAGCCAAAGCCGCCACGACAGACACCGCTCTGACTCCGAGTCCACGGAGAG CACCTGGGACATGTGGAACGAGAGGCTGGTGGAGATCGAGAAGGAGGCTGCCCGGAAGTATCGCTCCAAG AGCAAGAGGGAGGAGCTGGATGCCGATTCAGAGGCAGGGAGCAGGGTCCGAGAGGACGATGAGGAGAGTG TATTGTCCGAGGCCAGCTCCTTCTACAACTTCTGCAGTAGGAACAAGGACAAGCTCACTGCCCTGGAAAG GTGGAAGGTTAAGAGAATCCAATTTGGATTTCACAAGAAAGACTCAGAGGTGGTAGACGCGGGCAGTGAG GATGGTACGGAAGAGGCAACAGGAGAGAAGAACCTTTCTGATGTCAACCTGACAGCCTACCAGGCCTGGA AGCTGAAACACCAGAAGAAGGTGGGCAATGAGAACAAGGAGGAGGTGGTTGAGATGAGCAAAGGAGAGGA CACAGCCTTAGCTAAGAAGAGACAACGGAGACTAGAGTTACTGGAGAGAAGCAGGCAGACGCTGGAGGAG AGCCGGTCCATGGGAAGCTGGGAGGGAGACAGCTCAACTGCCAGCAGGAGCATCCCCCTGTCTGCATTCT GGTCTGCAGCCCCCTCTGTCAGTGTGGATGGGGACACAGCGTCAGTACTCAGCGCCCAGAGCCAGCATTC TCACCTGTCTCAGCCTGCAAGTAACATGCCCACCACACCCCTGCCTAACCTGCCAGTGGGTCCGGGAGAC ACCATTTCCATTGCCAGTATCCAGAACTGGATCGCCAGTGTCGTCAATGAAACCCTTGCCCAAAAGCAAA ACGAAATGCTCACGTTGTCCCGCGCCCCCTCTGTTGCAAGCATGAGGGCAGCTCCAGCAGCCAGCTGCCT TGGGGATGACCAACTCTCGGTGCTCAGCACCTCCCTGAGTGGCTGCCTACCGCCTCAGAGCCAGGGGAGA CCCAGCTCCGACGTGCAATCTGTGCTGTCCTCCACCAGCTCCCTGACCTCCAGGGCTGAAGGCAGTGAGA ACAAAGTGAGAGGGACCAGCAAGCCCATCTACAGCCTCTTTGCTGACAACGTGAACCTGAAGGAGCTCGG CCGGAAGGAGAAAGAAATGCAGATGGAGCTGCAGGAGAAGATGTCCGAATATAAAATGGAGAAGCTGGCC TCTGATAACAAGCGTAGCTCTCTCTTCAAGAAGAAGAAGGCCAAGGACGACGAGGACATCAGCGTGGGTG ACAGAGACGAGGACACAGACAGTGCCATTGGAAGCTTCCGGTATTCCTCCCGAAGTAATTCCCAGAAGCC TGAGACAGATGCCTCCTCCTCTCTGGCCATCTCCGATCACTTTAGAAATGGCCGCAGCGTGGGCAACGAG GTAGGTAGCAATATTAATACATGGCTCAGTGGCCTCAGGATGGAAGAAAAATCTCCTCCTCAAAGTGATT GGTCTGGAAGCTCCAGGGGGAGGTACTCCAGGTCTTCCCTGTTCCGGGAAACAGAGTCTAAATCCTGCAG TTACAAGTTCTCCAAATCTAGGTCACAGGAACAGGACTCCTCCTTCCACGAAACCGATGGCAACACTGTG CGAAACACCTCCCGGTTCTCCTCTTCCACCACCAAGGAGGGCAGAGAGATGCACAAGTTCTCCAGGTCCA CATTCAGCGAGACCTCGAGCTCCCGAGAGGAGAGCCCAGAACCCTATTTCTTCCGCCGAACCCCTGAACC GTCCGATGGGGAAGAGTCCCCCGAGCTAAGGCGTCCAAACTGGACCAGGCCCAGGGATTGGGAAGACGTA GAAGAGTCATCTAAATCAGACTTCGCTGAGTTTGGGGCCAAGAGGAAATTCACCCAGAGCTTCATGAGGT CTGAAGAGGAGGGAGAGAAGGAGAGGACAGAAAACCGAGAGGAAGGGAGGTTTGCATCTGGGCGTCAGTC CCAGTATCGGAGAAGCACAAACCGGCAAGAGGAAGAAGAAATGGATGATGAAGCCATCATTGCTGCTTGG AGAAAGCGGCAAGAAGAAACCAGAACCAAACTGCAAAGAAGGAGAGAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217497 representing NM_001191929 Red=Cloning site Green=Tags(s) MATSGDTEEEQVVPNEEDEADVRAVQAHYLRSPSPSQYSVVSDAETESIFMEPIHLSSAVAAKQIINEEL KPRGLRTDTECPGMLESAEQLLVEDLYNRVREKMDDKSLFNTPCVLDLQRALIQDRQEAPRNEVDEVWPN VFIAEKSVAVNKGRLKRLGITHILNAAHGTGVYTGPEFYTGLEIQYLGVEVDDFPEVDISQHFRKAAEFL DEALLTYRGKVLVSSEMGISRSAVLVVAYLMIFHNMAILEALMTVRRKRAIYPNDGFLKQLRELNEKLME EREEEDDEEEPEEDAGSTLGARVNSLMVEEEDDATSHLSGSSLGKASQVSQPVTLIDEEEEERLYEEWRK GQGFPKAEASQGRKGSCSASSEQDGDDCEDGAVERIIQEWQSRNERYQAKGHKQWNREEDEEEENSYTSR RRRHTLSESSASESVSSHDIRVLKQQLKRTSQSRHDRHRSDSESTESTWDMWNERLVEIEKEAARKYRSK SKREELDADSEAGSRVREDDEESVLSEASSFYNFCSRNKDKLTALERWKVKRIQFGFHKKDSEVVDAGSE DGTEEATGEKNLSDVNLTAYQAWKLKHQKKVGNENKEEVVEMSKGEDTALAKKRQRRLELLERSRQTLEE SRSMGSWEGDSSTASRSIPLSAFWSAAPSVSVDGDTASVLSAQSQHSHLSQPASNMPTTPLPNLPVGPGD TISIASIQNWIASVVNETLAQKQNEMLTLSRAPSVASMRAAPAASCLGDDQLSVLSTSLSGCLPPQSQGR PSSDVQSVLSSTSSLTSRAEGSENKVRGTSKPIYSLFADNVNLKELGRKEKEMQMELQEKMSEYKMEKLA SDNKRSSLFKKKKAKDDEDISVGDRDEDTDSAIGSFRYSSRSNSQKPETDASSSLAISDHFRNGRSVGNE VGSNINTWLSGLRMEEKSPPQSDWSGSSRGRYSRSSLFRETESKSCSYKFSKSRSQEQDSSFHETDGNTV RNTSRFSSSTTKEGREMHKFSRSTFSETSSSREESPEPYFFRRTPEPSDGEESPELRRPNWTRPRDWEDV EESSKSDFAEFGAKRKFTQSFMRSEEEGEKERTENREEGRFASGRQSQYRRSTNRQEEEEMDDEAIIAAW RKRQEETRTKLQRRRED TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191929
ORF Size	3411 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191929.1, NP_001178858.1
RefSeq Size	3899 bp
RefSeq ORF	3414 bp
Locus ID	498267
MW	128.7 kDa

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