Trpm5 (NM_001191896) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217513 representing NM_001191896 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGATGGCCCAGAGCTCTTGTCCTGGAAGCCCCCCAGATACTGGGGATGGATGGGAGCCAGTCCTAT GCAAGGGAGAGGTCAACTTCGGAGGGTCTGGGAAAAAGCGAAGCAAGTTTGTGAAGGTGCCAAGCAATGT GGCCCCCTCCATGCTCTTTGAACTCCTGCTCACCGAGTGGCACCTGCCAGCCCCCAACCTGGTGGTGTCC CTGGTGGGCGAGGAACGGCTTTTTGCTATGAAGTCCTGGCTTCGGGATGTCTTGCGCAAGGGGCTGGTGA AAGCAGCTCAGAGCACAGGTGCCTGGATCCTGACCAGTGCCCTCCATGTGGGCCTGGCACGCCATGTTGG ACAGGCTGTACGTGATCACTCTCTGGCTAGCACGTCCACCAAGGTCCGTGTGGTGGCCATCGGAATGGCC TCTCTGGACCGAATCCTTCACCGCCAACTTCTAGATGGTGTCCAGGAGGATACTCCCATCCACTACCCAG CAGATGAGGGCAGCACTCAGGGACCCCTCTGCCCTCTGGACAGCAATCTCTCCCACTTCATCCTCGTGGA GCCAGGCACCCTTGGGAGTGGGAACGACGGACTGGCAGAGCTGCAGCTGAGCCTGGAGAAGCACATCTCT CAGCAGAGGACAGGTTATGGGGGTACCAGCAGCATCCAGATACCTGTCCTTTGCTTGCTAGTCAATGGTG ACCCCAGCACCCTAGAGAGGATGTCCAGGGCAGTGGAGCAGGCTGCCCCATGGCTGATCCTGGCAGGTTC TGGGGGCATTGCTGATGTACTCGCTGCCCTGGTGGGCCAGCCTCATCTCCTGGTGCCCCAGGTGACCGAG AAGCAGTTCAGAGAGAAATTCCCAAGCGAGTGTTTCTCTTGGGAAGCCATTGTACACTGGACAGAGCTGC TACAGAACATTGCTGCACACCCCCACCTGCTCACAGTGTACGACTTTGAGCAGGAGGGTTCCGAGGACCT GGACACCGTCATCCTCAAGGCACTTGTGAAAGCCTGCAAGAGTCACAGCCGAGACGCACAAGACTACCTA GATGAGCTCAAGTTAGCAGTGGCCTGGGATCGCGTGGACATTGCCAAGAGTGAAATCTTCAATGGGGACG TGGAGTGGAAGTCCTGTGACTTGGAAGAGGTGATGACAGATGCCCTAGTGAGCAACAAGCCTGACTTCGT GCGCCTCTTTGTGGACAGTGGTGCTGACATGGCCGAGTTCTTGACCTATGGGCGGCTGCAGCAGCTTTAC CACTCTGTGTCCCCCAAGAGCCTCCTCTTTGAACTGCTGGAGCGTAAGCATGAGGAGGGTCGGCTGACAC TGGCTGGCCTGGGTGCCCAGCAGACCCGGGAGCTGCCCGTTGGTCTGCCTGCCTTTTCACTCCATGAGGT CTCCCGAGTTCTCAAAGATTTCCTGCATGACGCCTGCCGTGGCTTCTACCAGGATGGGCGCAGGATGGAG GAGAGAGGGCCACCCAAGCGGCCTGCAGGCCAGAAATGGCTGCCGGACCTCAGTCGGAAGAGTGAAGACC CATGGAGGGACCTGTTCCTTTGGGCTGTGCTGCAGAACCGTTATGAGATGGCCACATACTTCTGGGCCAT GGGCCGGGAGGGTGTGGCTGCTGCTCTGGCGGCCTGCAAGATCATCAAGGAAATGTCCCACCTGGAGAAA GAGGCAGAGGTGGCCCGCACTATGCGTGAGGCCAAGTATGAGCAGCTGGCCCTCGATCTTTTCTCAGAGT GCTACAGCAACAGTGAGGACCGTGCCTTTGCCCTGTTGGTGCGCAGGAACCACAGCTGGAGCAGGACCAC CTGCCTGCACCTGGCCACTGAGGCCGATGCCAAGGCCTTCTTTGCCCATGATGGTGTGCAAGCATTCCTG ACGAAGATCTGGTGGGGAGACATGGCCACAGGCACACCCATCTTACGACTTCTGGGTGCCTTCACCTGCC CAGCCCTCATCTACACAAATCTCATCTCCTTCAGTGAGGATGCCCCGCAGAGGATGGACCTGGAAGATCT GCAGGAGCCAGACAGTTTGGATATGGAAAAGAGCTTCCTGTGCAGCCATGGTGGCCAATTGGAGAAGTTA ACAGAGGCGCCAAGGGCTCCTGGCGATCTAGGCCCACAAGCTGCCTTCCTGCTCACACGGTGGAGGAAGT TCTGGGGCGCTCCTGTGACTGTGTTCTTGGGGAATGTGGTCATGTACTTTGCATTCCTCTTCCTATTCTC CTACGTCCTGCTGGTGGATTTCAGGCCACCACCCCAGGGGCCATCTGGGTCGGAAGTTACCCTGTATTTC TGGGTCTTCACACTGGTGCTGGAGGAAATCCGACAGGGATTCTTCACAAACGAGGACACCCGTCTGGTGA AGAAGTTCACTCTGTACGTAGAAGACAACTGGAACAAATGTGACATGGTGGCCATCTTCCTGTTCATTGT TGGTGTCACCTGTAGAATGGTGCCCTCCGTGTTTGAGGCTGGCCGGACTGTTCTGGCCATTGACTTCATG GTGTTCACACTTCGGCTCATCCACATCTTTGCTATTCACAAGCAGCTGGGTCCTAAGATCATCATTGTAG AGCGGATGATGAAAGATGTCTTCTTCTTCCTCTTCTTCCTGAGCGTGTGGCTCGTGGCCTATGGCGTGAC CACTCAGGCCCTGCTGCACCCCCACGATGGCCGTCTGGAGTGGATTTTCCGCCGTGTGCTCTACAGGCCT TACCTGCAGATCTTTGGGCAAATCCCTCTGGATGAAATTGATGAGGCCCGTGTGAACTGCTCTCTTCACC CGTTGCTGCTGGACAGCTCAGCTTCCTGCCCTAATCTCTATGCCAACTGGCTGGTCATTCTCCTGCTGGT TACCTTCCTCCTCGTCACTAATGTGCTACTTATGAACCTTCTGATCGCCATGTTCAGCTACACATTCCAG GTGGTGCAGGGCAATGCAGACATGTTCTGGAAGTTTCAACGCTACCACCTCATCGTTGAATACCACGGAA GGCCGGCTCTGGCCCCGCCCTTCATCCTGCTCAGCCACCTGAGCCTGGTGCTCAAGCAGGTCTTCAGGAA GGAAGCCCAGCACAAACAGCAACACCTGGAGAGAGACTTGCCTGACCCCGTGGACCAGAAGATCATTACC TGGGAAACAGTTCAAAAGGAGAACTTCCTGAGTACCATGGAGAAACGGAGGAGGGACAGTGAGGAGGAGG TGCTGAGGAAAACGGCACACAGAGTGGACTTGATTGCCAAATACATCGGGGGTCTGAGAGAGCAAGAAAA GAGGATCAAGTGTCTGGAGTCACAGGCCAACTACTGTATGCTCCTCTTGTCCTCCATGACTGACACACTG GCTCCTGGAGGCACCTACTCAAGTTCTCAAAACTGTGGTCGCAGGAGTCAGCCAGCCTCTGCTAGAGACA GGGAGTACCTAGAGGCTGGCTTGCCACACTCAGACACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217513 representing NM_001191896 Red=Cloning site Green=Tags(s) MPMAQSSCPGSPPDTGDGWEPVLCKGEVNFGGSGKKRSKFVKVPSNVAPSMLFELLLTEWHLPAPNLVVS LVGEERLFAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKVRVVAIGMA SLDRILHRQLLDGVQEDTPIHYPADEGSTQGPLCPLDSNLSHFILVEPGTLGSGNDGLAELQLSLEKHIS QQRTGYGGTSSIQIPVLCLLVNGDPSTLERMSRAVEQAAPWLILAGSGGIADVLAALVGQPHLLVPQVTE KQFREKFPSECFSWEAIVHWTELLQNIAAHPHLLTVYDFEQEGSEDLDTVILKALVKACKSHSRDAQDYL DELKLAVAWDRVDIAKSEIFNGDVEWKSCDLEEVMTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQLY HSVSPKSLLFELLERKHEEGRLTLAGLGAQQTRELPVGLPAFSLHEVSRVLKDFLHDACRGFYQDGRRME ERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHLEK EAEVARTMREAKYEQLALDLFSECYSNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQAFL TKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSHGGQLEKL TEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFSYVLLVDFRPPPQGPSGSEVTLYF WVFTLVLEEIRQGFFTNEDTRLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRMVPSVFEAGRTVLAIDFM VFTLRLIHIFAIHKQLGPKIIIVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVLYRP YLQIFGQIPLDEIDEARVNCSLHPLLLDSSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQ VVQGNADMFWKFQRYHLIVEYHGRPALAPPFILLSHLSLVLKQVFRKEAQHKQQHLERDLPDPVDQKIIT WETVQKENFLSTMEKRRRDSEEEVLRKTAHRVDLIAKYIGGLREQEKRIKCLESQANYCMLLLSSMTDTL APGGTYSSSQNCGRRSQPASARDREYLEAGLPHSDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191896
ORF Size	3468 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191896.1, NP_001178825.1
RefSeq Size	3471 bp
RefSeq ORF	3471 bp
Locus ID	365391
MW	130.6 kDa

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