Adcy6 (NM_001270785) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AC6; ACVI; ADCYB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217528 representing NM_001270785 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCTGCCCGTGGCCCGATCCGGTTCTGGGCGCAGCAGCATGTCATGGTTTAGCGGCCTCCTGGTTC CCAAAGTGGATGAACGGAAAACAGCCTGGGGCGAACGCAATGGACAGAAGCGCCCACGCCAGGCGACCCG AGCCCGTGGCTTCTGCGCGCCCCGCTACATGAGCTGCCTCAAGAATGTGGAGCCACCCAGCCCCACTCCT GCAGCTCGCACTCGGTGCCCCTGGCAGGATGAAGCCTTCATCAGGAGGGCTGGCCCGGGAAGGGGTGTGG AGCTGGGGCTGCGGTCAGTGGCCTTGGGTTTTGATGACACTGAGGTGACCACACCGATGGGGACAGCTGA AGTGGCACCCGACACATCGCCTCGAAGCGGTCCGTCCTGCTGGCACCGGCTAGCGCAGGTGTTCCAGTCT AAGCAGTTCCGCTCCGCCAAGCTGGAGCGTCTGTACCAGCGGTACTTCTTCCAGATGAACCAGAGCAGCC TCACGCTGCTCATGGCGGTGCTTGTGCTCCTCATGGCTGTACTGTTGACCTTCCACGCCGCGCCTGCCCT GCCTCAGCCTGCTTATGTGGCCCTGCTGACCTGTGCCTCCGTCCTTTTTGTGGTACTCATGGTAGTGTGT AACCGACATAGCTTCCGCCAGGACTCCATGTGGGTAGTGAGCTATGTGGTTCTGGGCATCCTAGCAGCCG TGCAAGTCGGGGGTGCCCTGGCAGCCAACCCACGCAGCCCCTCAGCAGGCCTTTGGTGCCCCGTGTTCTT CGTCTACATCACCTACACACTTCTTCCCATTCGTATGCGAGCGGCCGTGCTCAGTGGCCTGGGTCTTTCC ACCCTGCATTTGATTTTGGCCTGGCATCTCAACAATGGTGACCCCTTCCTTTGGAAGCAGCTCGGTGCTA ACGTGGTGCTCTTCCTGTGCACCAATGCCATCGGTGTCTGCACGCACTACCCCGCTGAAGTGTCTCAGCG CCAAGCCTTTCAGGAGACCCGTGGTTACATCCAGGCCCGGCTGCACTTGCAGCATGAGAATCGACAGCAG GAACGGCTGCTGCTGTCGGTGTTGCCCCAGCATGTTGCCATGGAAATGAAAGAGGATATCAACACAAAAA AGGAAGACATGATGTTCCACAAGATTTACATCCAGAAGCATGACAATGTCAGCATCCTGTTTGCCGACAT CGAGGGCTTCACCAGCCTGGCCTCCCAGTGCACTGCCCAGGAACTGGTCATGACCTTGAATGAGCTCTTT GCCCGGTTCGACAAGCTGGCTGCGGAGAATCACTGTCTGAGGATCAAGATCTTAGGAGACTGTTACTACT GTGTGTCGGGGCTGCCGGAGGCCCGGGCAGACCATGCCCACTGCTGTGTGGAGATGGGGGTAGACATGAT CGAGGCCATCTCGCTGGTGCGTGAGGTAACGGGTGTAAATGTGAACATGCGCGTGGGCATCCACAGCGGG CGTGTACACTGCGGTGTCCTTGGTCTGCGGAAATGGCAGTTTGATGTCTGGTCCAACGATGTGACCCTGG CCAACCACATGGAGGCGGGGGGCCGGGCCGGCCGCATCCACATCACTCGGGCCACACTGCAGTACCTGAA CGGGGACTATGAGGTGGAGCCAGGCCGTGGCGGTGAGCGCAACGCGTACCTCAAGGAGCAGTGCATTGAG ACCTTCCTCATACTAGGAGCCAGCCAGAAACGGAAAGAGGAGAAGGCCATGCTGGTCAAGCTGCAGCGGA CGCGGGCCAACTCCATGGAAGGACTGATGCCCCGCTGGGTTCCTGACCGTGCCTTCTCCCGGACCAAGGA CTCTAAGGCATTCCGACAGATGGGCATCGATGACTCTAGCAAAGAGAACCGGGGTGCCCAAGATGCTCTG AACCCTGAGGATGAGGTGGACGAGTTTCTGGGCCGAGCCATCGATGCCCGAAGCATCGACCAGCTGCGTA AGGACCATGTGCGCCGGTTCCTGCTCACCTTCCAGAGGGAGGATCTCGAGAAGAAGTATTCACGGAAAGT AGACCCTCGTTTCGGAGCCTACGTCGCCTGTGCCCTCCTGGTTTTCTGCTTCATCTGTTTCATCCAGTTC CTCGTATTCCCACACTCCGCCCTGATACTCGGGATTTATGCCGGGATCTTCCTTTTGCTGCTGGTCACCG TGCTCATCTGTGCTGTGTGCTCCTGTGGGTCTTTCTTCCCCAACGCCCTGCAGCGCCTGTCCCGCAGTAT CGTCCGCTCACGGGTGCACAGCACGGCTGTTGGAGTCTTCTCGGTTCTGCTTGTGTTCATCTCTGCCATT GCCAACATGTTCACCTGCAGTCACACCCCACTGAGGACCTGTGCGGCCCGGATGCTGAACTTAACACCGT CCGATGTCACCGCCTGCCACCTACGACAGCTCAATTACTCTCTGGGACTGGAAGCTCCCCTGTGTGAGGG CACCGCACCCACCTGCAGCTTCCCTGAGTACTTTGTCGGGAGTGTGCTGCTGAGTCTCTTGGCCAGCTCC GTCTTCCTCCACATCAGCAGCATTGGCAAGCTAGTTATGACCTTTGTCTTGGGGTTCATCTACTTGCTTC TGCTTTTGCTGGGTCCCCCAGCCACCATCTTTGACAACTATGATCTACTGCTTAGCGTCCATGGCTTGGC TTCCTCCAATGAGACCTTCGATGGGCTGGACTGCCCAGCCGTAGGGAGGGTAGCGCTCAAATACATGACC CCTGTGATTCTCCTCGTGTTCGCCCTGGCACTGTATCTACACGCACAACAGGTGGAATCTACCGCCCGCC TGGACTTCCTGTGGAAACTGCAGGCCACAGGGGAGAAGGAGGAGATGGAGGAGTTGCAGGCCTACAACCG GCGGCTGCTGCATAACATCCTTCCCAAGGACGTGGCTGCCCACTTCCTGGCCCGGGAGCGCCGCAACGAC GAGCTGTACTACCAATCCTGCGAGTGCGTGGCTGTCATGTTTGCCTCCATCGCCAACTTCTCTGAGTTCT ATGTGGAACTGGAGGCGAACAATGAGGGCGTGGAGTGCCTGCGACTGCTCAATGAGATCATCGCGGACTT TGATGAGATCATCAGTGAGGAGAGGTTCCGGCAGCTGGAGAAGATCAAGACCATCGGTAGCACTTACATG GCCGCCTCCGGGCTAAATGCCAGCACCTATGACCAGGTCGGCCGCTCGCACATCACCGCCCTGGCAGACT ACGCCATGCGGCTTATGGAGCAAATGAAACACATCAACGAACACTCTTTCAACAACTTCCAGATGAAGAT CGGGTTGAACATGGGTCCGGTTGTAGCAGGTGTCATTGGGGCCCGGAAGCCACAGTATGACATCTGGGGA AACACGGTGAATGTTTCCAGCCGTATGGACAGCACAGGAGTTCCTGACCGAATACAGGTGACCACGGATC TGTACCAGGTTCTAGCTGCCAAGGGCTACCAACTGGAGTGTCGAGGGGTGGTCAAGGTGAAGGGAAAGGG GGAGATGACCACCTACTTCCTCAATGGGGGCCCCAGCAGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217528 representing NM_001270785 Red=Cloning site Green=Tags(s) MPLPVARSGSGRSSMSWFSGLLVPKVDERKTAWGERNGQKRPRQATRARGFCAPRYMSCLKNVEPPSPTP AARTRCPWQDEAFIRRAGPGRGVELGLRSVALGFDDTEVTTPMGTAEVAPDTSPRSGPSCWHRLAQVFQS KQFRSAKLERLYQRYFFQMNQSSLTLLMAVLVLLMAVLLTFHAAPALPQPAYVALLTCASVLFVVLMVVC NRHSFRQDSMWVVSYVVLGILAAVQVGGALAANPRSPSAGLWCPVFFVYITYTLLPIRMRAAVLSGLGLS TLHLILAWHLNNGDPFLWKQLGANVVLFLCTNAIGVCTHYPAEVSQRQAFQETRGYIQARLHLQHENRQQ ERLLLSVLPQHVAMEMKEDINTKKEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELF ARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGVDMIEAISLVREVTGVNVNMRVGIHSG RVHCGVLGLRKWQFDVWSNDVTLANHMEAGGRAGRIHITRATLQYLNGDYEVEPGRGGERNAYLKEQCIE TFLILGASQKRKEEKAMLVKLQRTRANSMEGLMPRWVPDRAFSRTKDSKAFRQMGIDDSSKENRGAQDAL NPEDEVDEFLGRAIDARSIDQLRKDHVRRFLLTFQREDLEKKYSRKVDPRFGAYVACALLVFCFICFIQF LVFPHSALILGIYAGIFLLLLVTVLICAVCSCGSFFPNALQRLSRSIVRSRVHSTAVGVFSVLLVFISAI ANMFTCSHTPLRTCAARMLNLTPSDVTACHLRQLNYSLGLEAPLCEGTAPTCSFPEYFVGSVLLSLLASS VFLHISSIGKLVMTFVLGFIYLLLLLLGPPATIFDNYDLLLSVHGLASSNETFDGLDCPAVGRVALKYMT PVILLVFALALYLHAQQVESTARLDFLWKLQATGEKEEMEELQAYNRRLLHNILPKDVAAHFLARERRND ELYYQSCECVAVMFASIANFSEFYVELEANNEGVECLRLLNEIIADFDEIISEERFRQLEKIKTIGSTYM AASGLNASTYDQVGRSHITALADYAMRLMEQMKHINEHSFNNFQMKIGLNMGPVVAGVIGARKPQYDIWG NTVNVSSRMDSTGVPDRIQVTTDLYQVLAAKGYQLECRGVVKVKGKGEMTTYFLNGGPSS TRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-NotI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270785
ORF Size	3540 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270785.1, NP_001257714.1
RefSeq Size	5878 bp
RefSeq ORF	3543 bp
Locus ID	25289
MW	131.9 kDa
Gene Summary	may play a role in intracellular signaling [RGD, Feb 2006]

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