Diaph1 (NM_001107393) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Diap1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217569 representing NM_001107393 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGTCCGGCGGGGGCCTAGGGCCCGGCCGCGGGACCCGGGACAAGAAGAAGGGTCGGAGCCCGG ATGAGCTGCCTGCGACGGGCGGCGACGGCGGCAAACATAAGAAATTTACTTTGAAGCGGCTCATGGCAGA CGAGCTGGAGAGATTTACCAGCATGAGGATTAAGAAGGAGAAGGAAAAGCCCAATTCTGCTCATAGAAAC TCCTCTGCATCGTATGGAGATGACCCCACTGCTCAGTCACTGCAGGACATCTCAGATGATCAAGTTCTTG TCCTCTTTGAGCAGATGCTGGTGGATATGAACCTGAATGAGGAGAAACAGCAGCCTTTGCGAGAGAAGGA CATTGTCATCAAGAGGGAGATGGTGTCCCAGTATCTGCATACTTCCAAAGCTGGCATGAACCAGAAGGAG AGTTCCAGGTCTGCCATGATGTACATTCAGGAGCTGAGGTCAGGCTTGCGGGATATGCACCTGCTTAGCT GCCTGGAGTCCCTTCGAGTCTCTCTCAACAATAACCCTGTCAGTTGGGTGCAGACATTTGGTGCTGAGGG CCTAGCCTCCTTATTGGACATCCTCAAACGACTCCATGATGAGAAAGAGGAGACTTCTGGAAACTACGAC AGCCGGAACCAGCATGAGATTATCCGCTGTTTGAAAGCTTTCATGAACAACAAGTTTGGAATCAAAACCA TGTTGGAGACGGAAGAAGGAATCCTACTGCTGGTCAGAGCCATGGATCCTGCTGTTCCCAATATGATGAT TGATGCAGCAAAGCTGCTGTCTGCCCTCTGTATCCTACCGCAGCCAGAGGACATGAATGAACGAGTTTTA GAGGCTATGACAGAGAGAGCTGAAATGGAAGAGGTTGAACGCTTCCAGCCACTGCTGGATGGGTTAAAAA GTGGGACGTCTATTGCACTCAAAGTGGGATGCCTACAGCTGATCAATGCTCTCATCACTCCCGCTGAGGA ACTTGACTTCCGGGTTCACATCCGAAGTGAGCTGATGCGCTTGGGACTGCATCAAGTGTTGCAGGAGCTT CGAGAGATTGACAATGATGATATGCGAGTACAGCTCAATGTGTTTGATGAACAAGGGGATGAAGATTTCT TCGATCTGAAGGGACGACTGGATGATATCCGCATGGAGATGGATGACTTTGGTGAAGTTTTCCAGATTAT TTTAAACACAGTGAAAGATTCCAAGGCAGAACCACACTTCCTGTCTATCTTGCAGCATCTCCTGTTGGTC CGAAATGATTATGAAGCTAGGCCGCAGTACTATAAATTGATTGAAGAATGTGTTTCTCAGATAGTTCTAC ACAAAAATGGAACTGATCCTGACTTCAAGTGCCGGCACCTGCAGATTGATATTGAGGGGTTGGTTGATCA AATGATTGATAAAACGAAGGTGGAAAAATCTGAGGCCAAAGCTACAGAGCTGGAAAAAAAGTTGGACTCA GAATTAACAGCCCGGCATGAGTTACAAGTAGAAATGAAAAAGATGGAAAATGACTTTGAGCAGAAACTTC AGGATCTTCAAGGAGAAAAGGATGCCTTGGATTCTGAAAAGCAGCAGATCACTACACAAAAACAAGACCT GGAAGCAGAGGTGTCCAAGCTGACAGGAGAGGTTGCCAAGCTGTCAAAAGAACTAGAAGATGCCAAGAAG GAAATGGCTTCTCTCTCTGCTGTGGCTGTTGCACCTTCAGTTTCTAGTAGTGCTGCTGTTCCCCAAGCCC CTCCTCTGCCTGGCACTTCAGGCACTGTTATTCCACCTCCCCCACCACCCCCTCTCCCTGGAGGTGCCGT CCCACCCCCTCCTCCTCCTCCTCTGCCTGCAGGTACTGGTATCCCTCCACCTCCTCCTTTACCTGGAGGT GCTTGTATATCCTCTTCCCCACAGTTGTTTGGCAGTACTGCCATTCCCCCACCTCCTCCTTTGCCTGGAG CTACTGCCATCCCCCCACCTCCCCCTTTGCCTGGAGATACTGCCATCCCCCCACCTCCCCCTTTGCCTGG AGNTACTGCCATCCCCCCTCCTCCTCCTTTGCCTGGAGCTACTGGTGTACCACCACCTCCTCCCCCTTTA CCTGGAAGTGTTGGTGTTCCCCCACCCCCTCCCTTGCCTGGAGGACCAGGACTGCCTCCTCCTCCTCCCC CCTTTCCTGGAGCACCTGGCATTCCCCCCCCACCGCCTGGTATGGGCGTGCCTCCACCTCCCCCCTTTGG ATTTGGGATTCCTGCAGCCCCAGTTCTGCCATTTGGATTAACCCCCAAAAAAGTTTATAAGCCAGAGGTG CAACTCCGGAGGCCAAACTGGTCCAAGTTCGTGGCTGAGGACCTTTCCCAGGACTGCTTCTGGACAAAGG TGAAGGAGGACCGCTTTGAGAACAATGAACTATTTGCCAAACTTACCCTTGCCTTCTCTGCCCAGACCAA GACTTCTAAAGCCAAGAAGGATCAAGAAGGTGGAGAAGAAAAGAAATCTGTTCAAAAGAAGAAAGTAAAA GAGCTGAAAGTGCTGGATTCAAAGACAGCCCAGAATCTCTCAATCTTTTTGGGTTCATTCCGTATGCCCT ATCAAGAGATTAAGAACGTTATCCTGGAGGTGAATGAGGCTGTTCTCACGGAGTCTATGATCCAGAACCT CATTAAGCAGATGCCAGAACCAGAGCAGTTAAAAATGCTTTCTGAACTGAAGGAGGAGTACGATGATCTG GCTGAGTCAGAGCAGTTCGGCGTGGTGATGGGCACGGTTCCCCGTCTTCGGCCTCGCCTCAATGCCATCC TTTTCAAGCTACAGTTCAGTGAGCAAGTTGAGAATATCAAGCCAGAGATTGTATCTGTCACTGCGGCATG TGAGGAGCTGCGTAAGAGTGAGAACTTCTCTAGCCTCCTGGAGCTCACACTGCTCGTTGGAAACTATATG AATGCGGGCTCCAGGAATGCTGGAGCTTTTGGCTTCAATATCAGCTTCCTTTGTAAGCTTCGAGACACCA AGTCTGCAGATCAGAAGATGACTCTGTTGCATTTCTTGGCTGAGTTATGTGAGACTGACCACCCCGATGT CCTCAAGTTTCCTGATGAGCTTGCCCATGTAGAGAAAGCCAGCAGAGTTTCTGCTGAGAACTTGCAGAAG AATTTAGATCAGATGAAGAAACAGATTGCTGATGTGGAACGCGATGTTCAAAATTTCCCAGCTGCCACAG ACGAAAAAGACAAGTTTGTTGAGAAGATGACTAGCTTTGTGAAGGATGCACAGGAACAGTATAACAAGCT ACGGATGATGCACTCCAACATGGAGACTCTCTATAAGGAGCTAGGCGACTACTTCGTCTTTGACCCTAAG AAGTTGTCTGTAGAAGAATTTTTTATGGATCTGCACAACTTTAGGAATATGTTTTTGCAAGCAGTCAAGG AGAACCAGAAGCGCCGGGAAACAGAAGAAAAGATGAGGAGAGCAAAACTAGCCAAGGAAAAGGCGGAAAA AGAGCGACTGGAGAAGCAGCAGAAGCGAGAGCAGCTCATAGACATGAATGCAGAGGGGGATGAGACAGGT GTGATGGACAGTCTTCTAGAAGCTCTGCAGTCAGGAGCAGCATTCCGACGGAAGAGAGGGCCCCGGCAGG TCAACAGGAAAGCTGGGTGTGCAGTCACATCTCTGCTAGCCTCGGAGCTGACCAAGGATGATGCCGTGGC TGCTAGTTCTGCTAAGGTGCCCAAGAAAAGTGAAGGAGTCACCACAATCCTTGAAGAAGCCAAGGAACTG GTTGGCCGTGCAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217569 representing NM_001107393 Red=Cloning site Green=Tags(s) MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRN SSASYGDDPTAQSLQDISDDQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKE SSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYD SRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVL EAMTERAEMEEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQEL REIDNDDMRVQLNVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLV RNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIEGLVDQMIDKTKVEKSEAKATELEKKLDS ELTARHELQVEMKKMENDFEQKLQDLQGEKDALDSEKQQITTQKQDLEAEVSKLTGEVAKLSKELEDAKK EMASLSAVAVAPSVSSSAAVPQAPPLPGTSGTVIPPPPPPPLPGGAVPPPPPPPLPAGTGIPPPPPLPGG ACISSSPQLFGSTAIPPPPPLPGATAIPPPPPLPGDTAIPPPPPLPGXTAIPPPPPLPGATGVPPPPPPL PGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGIPAAPVLPFGLTPKKVYKPEV QLRRPNWSKFVAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVK ELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDL AESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELTLLVGNYM NAGSRNAGAFGFNISFLCKLRDTKSADQKMTLLHFLAELCETDHPDVLKFPDELAHVEKASRVSAENLQK NLDQMKKQIADVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGDYFVFDPK KLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETG VMDSLLEALQSGAAFRRKRGPRQVNRKAGCAVTSLLASELTKDDAVAASSAKVPKKSEGVTTILEEAKEL VGRAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107393
ORF Size	3795 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107393.2, NP_001100863.2
RefSeq Size	5588 bp
RefSeq ORF	3798 bp
Locus ID	307483
MW	140.4 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.