Npc1 (NM_153624) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cdig2; SLC66A1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217572 representing NM_153624 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCGCACCACCCGGCCCTCGGCCTCCTGCTGCTGCTGCTGCTGTGCCCTGCGCAGGTGTTCTCAC AGTCCTGTGTCTGGTACGGAGAGTGTGGAGTCGCCTTTGGAGATAAGAGGTACAACTGTGAATATTCGGG CCCACCGAAACCCCTCCCAAAGGACGGCTATGACTTAGTGCAGGAACTCTGTCCAGGACTCTTCTTTGGC AATGTCAGTCTTTGCTGTGACGTTCAGCAGCTTCAGACACTGAAGAGCAACCTGCAGCTGCCCCTGCAGT TTCTGTCCAGGTGTCCGTCATGTTTTTATAACCTGATGACCCTGTTTTGTGAGCTAACATGTAGCCCGCA CCAGAGTCAGTTTCTGAATGTGACAGCAACTGAAGATTATTTCGACCCCGAGACTCGGGAAAATAAAACA AATGTAAAGGAATTAGAGTACTACGTCGGACGGAGCTTTGCGAATGCAATGTACAATGCCTGCCGAGATG TGGAGGCGCCTTCCAGTAATGAGAAGGCCTTAGGACTCCTGTGCGGAAGGGATGCCCGTGCCTGCAATGC CACCAACTGGATTGAGTACATGTTCAACAAAGACAACGGGCAAGCACCGTTTACCATCATCCCCGTATTT TCAGATCTTTCGGTTCTTGGAATGGAACCCATGAGGAATGCCACCAAAGGCTGCAACGAGTCTGTAGATG AGGTCACAGGGCCGTGTAGCTGCCAGGACTGCTCTGCCGTCTGCGGCCCCAAACCCCAGCCCCCACCGCC CCCTGTGCCCTGGAGGATCTTAGGTTTGGACGCCATGTATGTCATCATGTGGGTCGCCTACATGGCGTTC CTGGTCCTGTTCTTTGGAGGACTGCTGGCAGTGTGGTGCCACAGAAGGCGGTACTTTGTGTCCGAGTACA CCCCCATTGACAGCAACATTGCCTTTTCCTCGAATAGCAGTGACAAAGGGGAAGCCTCGTGCTGCGACCC ACTTGGTGTAGCGTTTGATGACTGTCTGAGGCGCATGTTCACAAAGTGGGGGGCTTTCTGTGTCCGGAAT CCCACCTGCATCATTTTCTTCTCGTTGGTCTTCATTGCTGCGTGCTCTTCTGGCCTGGTGTTTGTCCAGG TCACCACCAATCCCGTGGAGCTCTGGTCAGCCCCTCACAGTCAGGCCCGCTTGGAAAAGGAGTACTTTGA CAAGCACTTTGGGCCTTTCTTTCGTACGGAGCAGCTTATTATCCAGGCCCCCAACTCCAGCGTCCATATC TACGAGCCGTACCCTTCCGGAGCCGATGTGCCCTTCGGGCCTCCATTGAACAAAGAGATTCTGCACCAGG TTCTGGACTTACAGATCGCCATTGAAAGTATCACTGCGTCCTATAACAATGAAACCGTGACACTTCAGGA CATCTGTGTGGCCCCCCTCTCTCCATACAACAAGAACTGCACCATTATGAGTGTGTTAAATTACTTCCAG AACAGCCATGCGGTGCTGGACAACCAAGTAGGCGATGACTTCTTTGTCTATGCCGATTACCACACGCACT TTCTGTACTGTGTGAGGGCTCCCGCCTCCTTGAATGATACGAGTTTGCTCCACGGCCCTTGCCTGGGCAC ATTTGGAGGACCTGTGTTCCCGTGGCTTGTGTTGGGCGGCTATGATGATCAGAACTACAATAATGCCACC GCGCTTGTGATTACCTTCCCTGTCAATAATTACTATAATGACACAGAGAAGCTCCAGAGGGCCTGGGCCT GGGAGAAAGAATTTATTAACTTTGTGAAGAACTACAAGAATCCAAATCTGACCATTTCTTTCACTGCTGA GCGAAGCATTGAAGATGAACTCAATCGGGAAAGCAACAGTGACGTATTCACCGTCCTCATCAGCTATGCC GTGATGTTTCTGTACATTTCCCTCGCCCTGGGGCACATCCAGAGCTGGAACAGGGTCCTGGTGGATTCTA AAATCTCGCTGGGCATTGCGGGGATCCTGATAGTGCTGAGCTCGGTGACCTGCTCCCTGGGCATCTTCAG CTACATCGGAATGCCACTGACCCTCATCGTCATTGAGGTCATCCCGTTCCTGGTGCTGGCTGTTGGGGTG GACAACATCTTCATTCTAGTGCAGACCTACCAGAGAGATGAGCGTCTTCAGGAGGAGACGTTGGATCAGC AGCTGGGCAGGATCCTGGGGGAAGTGGCCCCAACCATGTTCCTTTCGTCCTTCTCTGAGACCTCGGCATT TTTCTTTGGGGCGCTGTCCTCGATGCCAGCTGTCCACACCTTCTCTCTGTTTGCGGGGATGGCCGTCCTC ATTGACTTCCTCCTTCAGATTACCTGCTTTGTGAGCTTCTTGGGGTTAGATATTAAGAGACAAGAGAAAA ACCGTCTGGACATCCTGTGCTGTGTCAGAGGCCCTGACGACGGACAAGAATCCCAAGCCTCCGAAAGCTA CCTGTTCCGCTTCTTCAAAAACGCCTTTGCCCCATTTCTGCTGACGGACTGGCTGCGGCCAGTTGTGATG GCCGTGTTTGTGGGCATTCTGTCATTCAGTATTGCAGTAGTGAACAAAGTAGAAATCGGATTGGATCAGT CTCTCTCGATGCCAAATGATTCGTATGTGATTGACTATTTCAAGTCACTCGGTCAGTACCTGCACTCGGG CCCACCCGTGTACTTCGTCCTGGAAGAGGGCTACAACTACAGTTCCCGCAGAGGGCAGAACATGGTGTGC GGTGGCATGGGCTGTGACAATGACTCCCTGGTGCAGCAGATATTTAACGCAGCAGAGCTGGACACCTACA CCCGCGTAGGCTTCGCTCCCTCATCCTGGATTGATGATTACTTTGACTGGGTCTCACCGCAATCCTCCTG CTGTAGACTCTACAATGTCACTCACCAGTTCTGCAATGCTTCTGTGATTGACCCAACCTGTGTCCGCTGC AGACCTCTGACTCCAGAGGGCAAACAGAGGCCTCAGGGGAAAGAATTCATGAAATTCCTGCCCATGTTCC TGTCTGATAACCCCAACCCCAAGTGTGGCAAAGGGGGACATGCTGCTTACAGTTCTGCTGTTAACATCAT GGGAGATGACACTTACATTGGGGCCACTTACTTCATGACCTACCACACCATTCTTAAGACCTCTGCTGAT TATATCGATGCCCTGAAGAAAGCTCGGCTTATAGCCAGTAACATCACGGAAACCATGCGTTCTAAGGGGA GTGATTATCGTGTGTTTCCTTACAGTGTGTTCTATGTCTTCTATGAACAATACCTGACCATTATTGATGA CGCCATCTTTAACCTAAGTGTGTCTCTGGGCTCCATATTTCTCGTGACCTTGGTGGCTCTGGGCTGTGAA CTGTGGTCTGCGGTCATCATGTGTCTCACCATAGCTATGATCCTGGTCAACATGTTTGGTGTTATGTGGC TGTGGGGCATCAGTCTGAACGCTGTTTCCTTGGTCAACTTGGTGATGACCTGTGGCATTTCTGTGGAGTT CTGCAGCCATATAACAAGAGCTTTCACCATGAGCACCAAAGGAAGCAGAGTGAGCAGGGCGGAAGAGGCA CTGGCCCACATGGGTAGTTCTGTATTCAGTGGAATCACACTTACAAAATTTGGAGGGATCGTGGTGTTGG CCTTTGCCAAGTCTCAAATTTTTGAGATCTTTTACTTCAGGATGTACTTAGCCATGGTCTTACTTGGAGC TACTCACGGACTAATATTTCTTCCCGTCTTACTCAGTTACATAGGGCCATCAGTGAATAAAGCTAAAAGA CACACCACTCAAGAGCGGTACAAAGGGACAGAGAGAGAACGGCTCCTCAATTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217572 representing NM_153624 Red=Cloning site Green=Tags(s) MGAHHPALGLLLLLLLCPAQVFSQSCVWYGECGVAFGDKRYNCEYSGPPKPLPKDGYDLVQELCPGLFFG NVSLCCDVQQLQTLKSNLQLPLQFLSRCPSCFYNLMTLFCELTCSPHQSQFLNVTATEDYFDPETRENKT NVKELEYYVGRSFANAMYNACRDVEAPSSNEKALGLLCGRDARACNATNWIEYMFNKDNGQAPFTIIPVF SDLSVLGMEPMRNATKGCNESVDEVTGPCSCQDCSAVCGPKPQPPPPPVPWRILGLDAMYVIMWVAYMAF LVLFFGGLLAVWCHRRRYFVSEYTPIDSNIAFSSNSSDKGEASCCDPLGVAFDDCLRRMFTKWGAFCVRN PTCIIFFSLVFIAACSSGLVFVQVTTNPVELWSAPHSQARLEKEYFDKHFGPFFRTEQLIIQAPNSSVHI YEPYPSGADVPFGPPLNKEILHQVLDLQIAIESITASYNNETVTLQDICVAPLSPYNKNCTIMSVLNYFQ NSHAVLDNQVGDDFFVYADYHTHFLYCVRAPASLNDTSLLHGPCLGTFGGPVFPWLVLGGYDDQNYNNAT ALVITFPVNNYYNDTEKLQRAWAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESNSDVFTVLISYA VMFLYISLALGHIQSWNRVLVDSKISLGIAGILIVLSSVTCSLGIFSYIGMPLTLIVIEVIPFLVLAVGV DNIFILVQTYQRDERLQEETLDQQLGRILGEVAPTMFLSSFSETSAFFFGALSSMPAVHTFSLFAGMAVL IDFLLQITCFVSFLGLDIKRQEKNRLDILCCVRGPDDGQESQASESYLFRFFKNAFAPFLLTDWLRPVVM AVFVGILSFSIAVVNKVEIGLDQSLSMPNDSYVIDYFKSLGQYLHSGPPVYFVLEEGYNYSSRRGQNMVC GGMGCDNDSLVQQIFNAAELDTYTRVGFAPSSWIDDYFDWVSPQSSCCRLYNVTHQFCNASVIDPTCVRC RPLTPEGKQRPQGKEFMKFLPMFLSDNPNPKCGKGGHAAYSSAVNIMGDDTYIGATYFMTYHTILKTSAD YIDALKKARLIASNITETMRSKGSDYRVFPYSVFYVFYEQYLTIIDDAIFNLSVSLGSIFLVTLVALGCE LWSAVIMCLTIAMILVNMFGVMWLWGISLNAVSLVNLVMTCGISVEFCSHITRAFTMSTKGSRVSRAEEA LAHMGSSVFSGITLTKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKR HTTQERYKGTERERLLNF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_153624
ORF Size	3834 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_153624.2, NP_705888.2
RefSeq Size	5161 bp
RefSeq ORF	3837 bp
Locus ID	266732
MW	143 kDa
Gene Summary	cadmium-inducible gene; transcription upregulated by cadmium [RGD, Feb 2006]

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