Cachd1 (NM_001191758) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1310770
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217577 representing NM_001191758 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGCGAGCCCGAGGAAGAGGAGACCGTAAGGACGGCCGCTCTGGTCCGTCGCTGTCCCCGCTGCC CTGGCTGGCCTGGGGCCCCGCGGCCGCCCTTCTGGCTGCTCTGCCTTGTGGCATGCTGGATCTTGGGCGC CGTGGCGGACGCCGACTTCTCCATCCTGGACGAGGCGCAAGTGCTAGCGAGCCAGATGCGGAGGCTGGCT GCCGAGGAGCTGGGGGTGGTCACCATGCAGAGGATCTTCAACTCCTTGGTTTACACAGAGAAGATCTCAA ACGGAGAGAGCGAAGTTCAGCAGCTAGCCAAAAAAATCCGGGAGAAGTTCAACCGTTACCTGGACGTGGT CAATCGGAACAAGCAAGTTGTAGAAGCCTCCTATACAGCCCACCTGACCTCCCCGCTGACCGCCATCCAG GACTGCTGCACAATTCCACCCTCCATGATGGAGTTTGATGGGAACTTCAATACCAATGTGTCTAGAACAG TGAGCTGCGATCGACTCTCTACTACTGTGAACAGCCGAGCCTTCAATCCAGGACGAGACTTAAATTCAGT TCTTGCAGACAACCTGAAATCTAACCCTGGAATCAAGTGGCAATATTTCAGCTCAGAAGAAGGAATTTTC ACTGTCTTCCCAGCACACAAGTTCCGGTGTAAGGGCAGCTATGAGCACCGCAGTCGGCCCATCTATGTCT CTACCGTCCGGCCGCAGTCCAAACACATAGTCGTGATCCTGGACCACGGGGCCTCAGTCACGGACACCCA GCTCCAGATTGCCAAGGACGCCGCTCAGGTCATCCTCAGTGCCATTGATGAACACGACAAGATTTCTGTA TTGACTGTGGCAGACACCGTCAGGACCTGTTCGTTGGACCAGTGCTATAAGACGTACCTGTCTCCAGCCA CCAGTGAGACAAAAAGGAAAATGTCCACATTTGTTAGCAGTGTCAAACCCTCCGACAGCCCCACCCAGCA CGCAGTAGGGTTTCATAGGGCGTTCCAGTTGATTCGAAGCACCAGCAATAACACTCGGTTCCAAGCAAAC ACAGACATGGTCATCATTTACCTGTCAGCGGGTATCACATCAAAGGACTCCTCGGAAGAGGATAAAAAGG CAACTCTCCGCGTCATCAACGAAGAAAATGGCTTCCTGAACAACTCTGTAATGATTCTTACCTACGCCCT CATGAATGATGGGGTGACTGGTTTGAAGGAACTGGCGTTCCTCAGGGATCTGGCTGAACAGAACTCTGGG AAGTATGGAATCCCAGACCGGACGGCCTTGCCTGTGATTAAGGGCAGCATGATGGTGCTGAACCAGCTGA GCAACCTGGAGACCACTGTTGGCAGGTTCTATACCAACCTTCCCAACCGGATGATTGACGAGGCCGTCTT CAGCCTCCCCTTCTCTGATGAGATGGGAGATGGTCTGATCATGACTGTGAGTAAACCCTGTTACTTTGGA AACCTTCTCCTGGGGATCGTAGGTGTGGATGTGAATCTGGCTTACATTCTCGAAGATGTGACATATTACC AAGACTCTTTAGCTTCCTATACTTTTCTCATAGATGACAAAGGGTACACACTTATGCACCCGTCTCTCAC CAGGCCATACTTACTGTCAGAGCCTCCCCTTCATACCGACATCATACATTATGAAAATATCCCCAAGTTT GAATTGGTTCGACAAAATATCCTAAGCCTCCCTCTAGGCAGCCAGATCATCACAGTGCCTGTTAACTCGT CCCTGTCTTGGCACATCAACAAGCTGAGGGAGACTGGAAAGGAGGCCTACAATGTGAGCTATGCCTGGAA GATGGTACAGGACACTTCCTTCATTCTGTGTATTGTGGTGATACAACCAGAAATACCTGTCAAACAATTG AAGAACCTCAACACCGTACCCAGCAGCAAGCTGCTGTACCACAGGCTAGACCTTCTGGGCCAACCCAGTG CTTGTCTCCACTTCAAGCAGCTGGCAACACTAGAAAGTCCAACCGTCATGCTGTCTGCCGGCAGCTTTTC CTCCCCCTATGAGCACCTCAGCCAGCCAGAGACCAAGCGCATGGTGGAGCATTACACGGCCTATCTCAGT GACAACACTCGCCTCATTGCTAACCCAGGGCTCAAATTCTCTGTCAGAAACGAAGTAATGGCGACCAGCC ACGTCACAGATGAATGGATGACACAAATGGAAATGAGCAGCCTGAACACGTACATTGTCCGCCGTTACAT AGCAACACCCAATGGCGTCCTCAGAATTTATCCTGGTTCCCTCATGGACAAAGCATTTGATCCCACGAGG AGACAGTGGTATCTTCATGCAGTAGCCAATCCAGGGCTGATTTCCTTGACGGGCCCTTACCTAGATGTTG GAGGAGCTGGCTATGTCGTGACAATCAGCCACACAATTCACTCATCCAGTACCCAGCTGTCTTCCGGACA CACTGTGGCTGTGATGGGCATCGACTTCACACTGAGGTACTTCTACAAGGTTCTGATGGATCTCTTACCA GTTTGTAACCAAGATGGTGGCAACAAAATAAGGTGCTTCATAATGGAGGACAGAGGTTATCTGGTAGCTC ATCCAACCCTCGTTGACCCCAAAGGTCATGCACCTCTGGAACAGCAACACATCACCCACAAGGAGCCCCT GGTAGCAAACGATATCCTGAACCATCCCAACTTTGTGAAGAAGAACTTGTGCAACAGCTTCAGTGACCGG ACAGTGCAGAGATCTTACAAGTTCAACACCAGCCTGGTGGGGGATTTGACAAACCTTGTGCATGGCAGCC ACTGTTCTAAATACCGCCTGACAAGGATCCCAGGAACCAACGCGTTTGTCGGCATCGTCAATGAGACCTG CGACTCTCTTGCCTTCTGCGCTTGCAGCATGGTGGACCGTCTCTGTCTCAACTGTCACCGAATGGAGCAA AATGAATGTGAATGTCCTTGTGAGTGCCCCCTGGAGGTCAATGAGTGTACTGGCAACCTCACCAATGCAG AGAACAGAAACCCGAGCTGTGAGGTCCACCAGGAGCCAGTGACATACACAGCTATCGATCCTGGCCTGCA AGATGCCCTGCACCAGTGTGTCAACAGTCGATGTAACCAGAGGATGGAAAGTGGGGACTGTTTCGGAGTG CTGGACTGCGAGTGGTGCGTGGTGGACAGTGACGGAAAGACTCACCTGGACAAATCCTACTGTGCGCCCC AGAAAGAATGCTTTGGAGGGATCGTGGGAGCCAAAAGTCCCTACGTTGATGACATGGGAGCCATCGGTGA TGAGGTGATCACACTGAACATGATTAAGAGCGCCCCAGTGGGCCCTGTGGCCGGAGGGATCATGGGCTGC ATCATGGTGCTGGTCCTTGCCGTCTACGCTTACCGCCACCAGATCCATCGCCGGAGCCACCAGCATATGT CTCCTCTTGCTGCTCAAGAAATGTCAGTGCGTATGTCCAACCTGGAGAATGACAGAGATGAAAGAGAGGA TGACAGTCACGAAGACAGAGGCATCATCAGCAACACTCGGTTCATAGCTGCAGTCATGGAGCGGCACGCA CACAGCCCGGAAAGGAGGCGACGCTACTGGGGCCGCTCGGGAACCGAAAGTGATCATGGGTACAGCACCA TGAGCCCGCAGGAAGACAGTGAGAACCCTCCATGCAACAATGACCCTTTGTCAGCTGGGGTGGATGTAGG GAACCATGATGACGACTTGGACCTGGACACCCCGCCTCAGACTGCAGCCCTGCTGAGCCACAAGTTCCAC CACTACCGCTCACACCACCCGACCCTCCATCACAGCCACCACCTGCAGGCGGCCGTGACAGTGCACACGG TGGATGCTGAGTGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217577 representing NM_001191758 Red=Cloning site Green=Tags(s) MAREPEEEETVRTAALVRRCPRCPGWPGAPRPPFWLLCLVACWILGAVADADFSILDEAQVLASQMRRLA AEELGVVTMQRIFNSLVYTEKISNGESEVQQLAKKIREKFNRYLDVVNRNKQVVEASYTAHLTSPLTAIQ DCCTIPPSMMEFDGNFNTNVSRTVSCDRLSTTVNSRAFNPGRDLNSVLADNLKSNPGIKWQYFSSEEGIF TVFPAHKFRCKGSYEHRSRPIYVSTVRPQSKHIVVILDHGASVTDTQLQIAKDAAQVILSAIDEHDKISV LTVADTVRTCSLDQCYKTYLSPATSETKRKMSTFVSSVKPSDSPTQHAVGFHRAFQLIRSTSNNTRFQAN TDMVIIYLSAGITSKDSSEEDKKATLRVINEENGFLNNSVMILTYALMNDGVTGLKELAFLRDLAEQNSG KYGIPDRTALPVIKGSMMVLNQLSNLETTVGRFYTNLPNRMIDEAVFSLPFSDEMGDGLIMTVSKPCYFG NLLLGIVGVDVNLAYILEDVTYYQDSLASYTFLIDDKGYTLMHPSLTRPYLLSEPPLHTDIIHYENIPKF ELVRQNILSLPLGSQIITVPVNSSLSWHINKLRETGKEAYNVSYAWKMVQDTSFILCIVVIQPEIPVKQL KNLNTVPSSKLLYHRLDLLGQPSACLHFKQLATLESPTVMLSAGSFSSPYEHLSQPETKRMVEHYTAYLS DNTRLIANPGLKFSVRNEVMATSHVTDEWMTQMEMSSLNTYIVRRYIATPNGVLRIYPGSLMDKAFDPTR RQWYLHAVANPGLISLTGPYLDVGGAGYVVTISHTIHSSSTQLSSGHTVAVMGIDFTLRYFYKVLMDLLP VCNQDGGNKIRCFIMEDRGYLVAHPTLVDPKGHAPLEQQHITHKEPLVANDILNHPNFVKKNLCNSFSDR TVQRSYKFNTSLVGDLTNLVHGSHCSKYRLTRIPGTNAFVGIVNETCDSLAFCACSMVDRLCLNCHRMEQ NECECPCECPLEVNECTGNLTNAENRNPSCEVHQEPVTYTAIDPGLQDALHQCVNSRCNQRMESGDCFGV LDCEWCVVDSDGKTHLDKSYCAPQKECFGGIVGAKSPYVDDMGAIGDEVITLNMIKSAPVGPVAGGIMGC IMVLVLAVYAYRHQIHRRSHQHMSPLAAQEMSVRMSNLENDRDEREDDSHEDRGIISNTRFIAAVMERHA HSPERRRRYWGRSGTESDHGYSTMSPQEDSENPPCNNDPLSAGVDVGNHDDDLDLDTPPQTAALLSHKFH HYRSHHPTLHHSHHLQAAVTVHTVDAEC SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191758
ORF Size	3864 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191758.1, NP_001178687.1
RefSeq Size	4559 bp
RefSeq ORF	3867 bp
Locus ID	298267
MW	143.9 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.