Mink1 (NM_001271136) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MEKKK 6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217598 representing NM_001271136 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACCCAGCCCCCGCCCGAAGCTTGGACGACATCGACCTGTCTGCCCTGAGGGACCCTGCAGGAA TCTTTGAGCTGGTGGAGGTGGTTGGCAATGGAACCTATGGACAGGTGTACAAGGGGCGGCACGTCAAGAC TGGGCAGCTGGCTGCCATTAAGGTCATGGATGTCACAGAGGATGAGGAGGAAGAAATCAAACAGGAAATC AACATGTTAAAGAAGTACTCTCACCATCGCAATATTGCCACCTACTACGGGGCCTTCATCAAGAAGAGCC CTCCTGGGAACGATGACCAGCTCTGGCTGGTGATGGAGTTCTGTGGTGCTGGCTCAGTGACCGACCTGGT AAAGAACACGAAAGGAAACGCGCTGAAAGAGGATTGCATTGCGTACATCTGCAGGGAGATTCTCAGGGGT CTTGCCCATCTCCATGCCCACAAGGTGATCCACCGAGATATCAAGGGACAAAATGTGCTGCTGACAGAGA ATGCTGAAGTCAAGCTAGTGGACTTTGGGGTGAGTGCTCAGCTGGACCGCACTGTGGGGAGGCGGAACAC TTTCATTGGGACCCCATACTGGATGGCTCCAGAAGTCATTGCCTGTGACGAGAACCCCGATGCCACCTAT GACTACAGGAGTGACATTTGGTCTCTAGGAATCACAGCCATTGAAATGGCAGAGGGAGCCCCCCCTCTGT GTGACATGCACCCTATGCGGGCTCTCTTCCTCATCCCTCGGAACCCTCCCCCCAGGCTCAAGTCAAAGAA ATGGTCTAAGAAGTTCACTGACTTCATTGACACGTGTCTCATCAAGACTTACCTGAGCCGACCACCAACA GAACAGTTACTCAAATTCCCCTTCATCCGAGACCAGCCCACGGAGCGGCAGGTCCGCATCCAGCTCAAGG ACCACATTGACCGCTCCCGGAAGAAGCGGGGGGAGAAAGAGGAGACAGAGTATGAGTACAGTGGCAGTGA AGAGGAAGACGACAGCCATGGAGAGGAAGGCGAGCCAAGTTCCATCATGAATGTGCCTGGGGAGTCCACA CTCCGCCGAGAATTCCTCAGACTCCAACAGGAGAATAAGAGCAACTCAGAGGCTTTAAAGCAGCAGCAGC AGCTGCAGCAGCAGCAGCAGCGGGACCCTGAGGCACACATCAAACACCTGCTGCACCAGCGGCAGCGTCG CATAGAGGAGCAGAAGGAGGAGCGGCGACGTGTAGAGGAGCAACAGCGGCGAGAGCGAGAACAGCGCAAG CTGCAGGAGAAGGAGCAGCAGCGGCGGTTGGAAGACATGCAAGCCCTTCGTCGAGAGGAAGAGAGGCGGC AAGCAGAGCGGGAACAGGAATACAAGCGGAAGCAGCTGGAGGAGCAGCGGCAGTCGGAGCGGCTCCAGAG ACAGCTGCAGCAGGAGCATGCCTACCTCAAGTCCCTGCAGCAGCAGCAGCAACAGCAGCAGCAGCTCCAG AAACAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGATCCTACCTGGAGACAGGA AACCCCTGTATCATTATGGTCGGGGCATTAATCCTGCTGACAAGCCAGCATGGGCCCGAGAGGTGGAAGA GAGAGCTCGGATGAACAAGCAGCAGAACTCTCCCTTGGCCAAGACAAAGCCAAGCAGTGCGGGGCCAGAG CCCCCCATTCCCCAGGCCTCTCCTAGCCCCCCAGGACCTCTTTCCCAAACTCCTCCTATGCAGAGGCCTG TGGAGCCTCAGGAAGGACCACACAAGAGCCTGGTGGCACACCGGGTCCCACTGAAGCCATATGCAGCACC TGTACCCCGATCCCAGTCCCTGCAGGACCAACCCACTCGAAACTTGGCTGCCTTCCCAGCCTCCCACGAC CCTGACCCTGCTGCTGTCCCCACACCCACTGCCACACCCAGTGCCCGAGGAGCTGTCATCCGCCAGAATT CAGACCCCACCTCTGAAGGGCCTGGGCCTAGCCCTAACCCTCCATCCTGGGTCCGGCCTGATAATGAAGC TCCACCCAAGGTTCCTCAGAGGACCTCGTCTATTGCCACTGCCCTTAACACCAGTGGGGCTGGAGGGTCC CGGCCAGCTCAGGCTGTCCGTGCCAGACCTCGCAGCAACTCCGCCTGGCAAATCTATCTGCAGAGGCGGG CAGAGCGAGGCACCCCCAAGCCTCCTGGGCCCCCAGCTCAGCCCCCTGGCCCGCCCAACACCTCTAGTAA CCCTGACCTCAGGAGGAGTGACCCTGGCTGGGAGCGTTCAGACAGTGTCCTCCCTGCCTCCCACGGCCAC CTCCCTCAGGCCGGCTCCTTGGAGCGGAACCGAAACCGTGTGGGAGCCTCCACAAAACTGGACAGCTCCC CAGTGCTCTCCCCTGGGAACAAGGCCAAGCCTGAAGACCACCGCTCAAGGCCAGGCCGGCCCGCAAGCTA TAAGCGAGCGATTGGTGAGGATTTCGTGTTGCTCAAAGAGCGGACTCTGGATGAGGCCCCTAAGCCTCCC AAGAAGGCCATGGACTATTCCTCATCCAGTGAGGAGGTGGAGAGCAGTGAAGATGAGGAGGAGGAAGGCG ATGGGGAGCCATCAGAGGGGAGCAGAGACACTCCCGGGGGCCGTGATGGTGACACAGATAGCGTCAGCAC CATGGTGGTTCATGATGTTGAAGAGGTATCCGGGACCCAGCCTTCATATGGAGGAGGCACCATGGTGGTC CAGCGTACTCCTGAAGAGGAGCGAAGCCTGCTGCTTGCTGATAGCAATGGCTACACAAACCTGCCAGATG TGGTCCAGCCCAGCCACTCACCTACTGAGAACAGCCAAGGTCAAAGCCCTCCAACAAAGGATGGAGGCGG TGATTACCAGTCTCGTGGGCTGGTAAAGGCCCCAGGAAAGAGTTCATTCACCATGTTTGTGGATCTAGGG ATCTACCAGCCTGGAGGCAGTGGGGATACCATCCCCATCACAGCCCTAGTGGGCGGAGAGGGTGGTCGCC TTGATCAACTGCAGTTCGATGTGAGGAAGGGCTCTGTGGTCAACGTCAATCCCACCAACACTCGAGCTCA TAGTGAAACTCCCGAGATTCGCAAGTACAAGAAGCGATTCAATTCCGAGATCCTCTGTGCAGCCCTCTGG GGGGTCAACCTCCTAGTGGGCACAGAGAATGGGCTGATGTTGCTGGACCGAAGTGGGCAGGGCAAAGTGT ATGGACTTATTGGGCGACGACGCTTCCAGCAAATGGATGTTCTGGAAGGGCTCAACCTGCTCATCACCAT CTCAGGGAAAAGGAACAAACTGCGGGTATATTACCTGTCCTGGCTTCGGAATAAGATTCTACACAATGAC CCAGAGGTGGAAAAGAAGCAGGGGTGGACCACCGTGGGGGACATGGAGGGCTGCGGCCACTATCGTGTTG TGAAATATGAACGAATTAAGTTCCTGGTCATTGCTCTGAAGAACTCTGTGGAGGTGTATGCCTGGGCCCC TAAACCCTACCACAAATTCATGGCCTTCAAGTCCTTTGCTGACCTCCCTCACCGCCCTCTGCTGGTGGAC CTGACAGTAGAGGAGGGCCAGCGGCTCAAGGTCATCTATGGCTCCAGTGCTGGCTTCCATGCTGTGGATG TTGACTCTGGGAACAGCTATGACATCTACATCCCTGTACATATCCAGAGCCAAATCACACCCCACGCCAT CGTCTTTCTCCCCAACACTGACGGCATGGAGATGCTGCTTTGCTATGAAGATGAGGGTGTCTACGTCAAC ACTTACGGGCGGATCATCAAGGACGTGGTGCTGCAGTGGGGAGAGATGCCCACCTCTGTCGCCTACATCT GCTCCAACCAGATAATGGGCTGGGGTGAGAAGGCCATAGAGATCCGCTCTGTGGAGACAGGCCACCTAGA TGGGGTCTTCATGCACAAACGAGCCCAGAGGCTCAAGTTCCTGTGTGAGCGGAATGATAAGGTGTTTTTT GCCTCAGTCCGCTCTGGAGGAAGCAGCCAAGTTTACTTTATGACTCTGAACCGTAACTGCATCATGAACT GG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217598 representing NM_001271136 Red=Cloning site Green=Tags(s) MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEI NMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRG LAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATY DYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPT EQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGEST LRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRK LQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQQLQ KQQQQQQQQQQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKTKPSSAGPE PPIPQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHD PDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGS RPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNTSSNPDLRRSDPGWERSDSVLPASHGH LPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPP KKAMDYSSSSEEVESSEDEEEEGDGEPSEGSRDTPGGRDGDTDSVSTMVVHDVEEVSGTQPSYGGGTMVV QRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSQGQSPPTKDGGGDYQSRGLVKAPGKSSFTMFVDLG IYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALW GVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHND PEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVD LTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIVFLPNTDGMEMLLCYEDEGVYVN TYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFF ASVRSGGSSQVYFMTLNRNCIMNW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271136
ORF Size	4062 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271136.1, NP_001258065.1
RefSeq Size	5008 bp
RefSeq ORF	4065 bp
Locus ID	303259
MW	152.5 kDa

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