Baz1a (NM_001170568) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217655 representing NM_001170568 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCTGCTTCACCGAAAGCCGTTCGTGAGGCAGAAGCCGCCGGGGGACCTGCGGCCGGACGAGGAAG TTTTCTACTGTAAAGTCACCAACGAGATCTTCCGCCACTACGATGACTTTTTTGAGCGAACCATTCTGTG CAACAGCCTTGTGTGGAGCTGCGCTGTGACAGGCAGACCTGGACTGACATATCAGGAAGCACTTGAGTCA GAGAGAAAAGCAAAGCAGGATCTCCAGAGTTTCCCAGAACCACTAATTATTCCAGTTTTATACTTGACCA ACCTTACCCATCGTTCACGCTTACATGAAATTTGCGATGATATCTTTGCATATATCAAGGACCGATATTT TGTTGAAGAAACTGTGGAAGTCATTAGGAACAACGGTACAAGGCTGCAGTGTAGAATCTTGGAAGTCCTC CCTCCATTACATCAAAATGGTTTTGCTAATGGACACATGAGCAGTGTTGACGGAGAGACTATTGTCATCA GTGACAGTGACGACTCAGAAACACAAAGCAGTTCTTTTCAACATGGGAAGAAGAAAGATGCAATCGATTC CTTATTATTCAGATACAGGGTACAGCCCACTAAAAAGGAGATGTATGAGTCTGCTGTTGTTAAAGCAACA CAAATCAGCCGGAGAAAACACCTATTTTCTCGTGATAAACTAAAGCTTTTTCTCAAGCAACACTGTGAAC CACAAGATGGAGTCATTAAAATTAAGGCATCATCTTTTTCAACATATAACATAGCAGAGCAAGATTTTTC TTATTTCTTCCCTGATGATCCACCTACATTTATCTTCAGTCCTGCTAACAGACGAAGAGGAAGACCTCCC AAACGAATATCTTTTGGTCAGGAAGACAACATTGCTAGTAAGCAGACTATTGCAAGATGTCGGAGCAAGG CTATTAAAGAAAGAGACAAACTTTTGAAACAAGAAGAAATGAGGGCACTAGCTTTTGAAAAGGCCAAACT AAAACGAGAAAAAGCAGATGCTTTGGAAGCAAGAAAAAGAGAAAGAGAAGATAGAGAGAAAAAGAGGGAA GAATTGAAGAAAATTGTAGAAGAAGAACGACTCAAGAAAAAAGAAGAAAAAGAGAGACTTAAAATAGAAA GAGAAAAGGAAAGAGAGAAATTACGTGAAGAAAAACGAAAATATATGGAATACTTAAAGCAGTGGAGTAA ACCCAGAGAAGACATGGAATGTGACGACCTTAAGGAACTTCCAGAACCAACTCCGGTGAAAACCAGACTA CCTCCTGAAGTCTTCGGTGATGCTCTGATGGTTTTGGAGTTCCTTAATGCATTTGGGGAACTTTTTGATC TTCAAGATGAGTTTCCTGAAGGAGTAACCTTAGAAGTGTTAGAGGAAGCTCTTGTAGGAAATGACAGTGA AGGCCCACTGTGTGAATTGCTTTTTTTCTTCCTGACTGCAATCTTCCAGGCGATGGCTGAAGAAGAGGAG GAAGTAGCCAAAGAGCAGATAACTGATGCTGACACCAAAGATCTAACAGAGGCTTTGGATGAGGACGCAG ACCCCACAAAATCTGCACTGTCTGCAGTTGCAGCCTTGGCCGCTGCATGGCCACAGTTACACCAGGGCTG CAGTTTGAAAAGCTTGGATCTTGATAGCTGCACTCTTTCTGAAATCCTCAGACTGCACATCTTAGCTTCA GGTGCTGATGTGACATCAGCAAATGCAAAGTACCGGTACCAGAAACGAGGAGGATTCGATGCCACAGATG ATGCCTGCATGGAGCTTCGACTGAGCAATCCCAGTCTGGTGAAAAAACTGTCCAGCACTGCTGTGTATGA TTTGACACCAGGAGAAAAAATGAAGATACTTCATGCTCTCTGTGGGAAGCTACTGACCCTAGTTTCTACT AGGGATTTCATTGAAGATTATGTTGATGTATTGCGACAGGCCAAGCAGGAGTTCCGAGAGCTCAAAGCAG AACAACACCGTAAAGAGAGAGAAGCCACAGCTGCTCGAATACGCAGAAGGAAGGAAGAAAAACTTAAGGA ACAAGAACAGAAAATGAAAGAGAAACAAGAGAAACTGAAGGAGGATGAACAGAGAACTTCAGCTGCAGAT CTAGGGGAAGAAGGAAGGGAAGATTTTGATACCAGTGCTGACAGCAAAGAAATAGAGCGGAAAGATCTGG ATCCTGATGTGGTTACTGAAGATGAAGACGACCTGGGATCACATAAAAGGAGCAGAAGAGGAAAAGCAGG ACAAACTGCAGTTAAACAATGTATAAAGCAAGAAGAGATGAACTACTGTATAAAGCAGGAGCCTCTGACT GCTGATGCAGAAGAAGCTTTGAGACAGGAGCACCAGCGGAAAGAGAAAGAACTCCTAGACAAGATCCAGA GTGCCATCGCCTGTACTAACATCTTCCCTCTCGGACGAGACCGCTTGTACAGGCGGTACTGGGTCTTCCC TTCTATCCCTGGTCTGTTTATAGAGGAGGATTACTCGGGTCTTACTGAAGACATGCTGTTGCCTAGGCCT TCATCATTCCACAGTAATGCACAGCCTCACAGTCCTCGGGTATCTATTAAAACTGAAGAGTCCTTCATGT CTGAATCTACCTCCAGCCTCGACCAGGGTCCATTCGATGATTCTCTGCTGCTGCCAAAACCAGTGCATAA GCCAAATCGGTGGTGCTTTTACAGTTCCTGTGAACAGCTAGACCAGCTCATTGAAGCTCTCAACTCCAGA GGACACAGAGAAAGTGCCTTAAAAGAGACCTTGCTTCAGGAGAAGAGCAGAATATGTGCACAGCTCGCAC ACTTTTCTGAAGAGAAATTCCACTTTTCAGACAAACCTCAGGCTGATAGCAAGCCCGTTTCTTCTCGGGG ACGATCTGCTGCATGTGACACATCACAGATGTCTGCGGAGAGGCAGCTTGAGCTGCGACTCAGAGACTTT CTCTTGGATATCGAAGACAGGATCTATCAAGGGACACTAGGAGCCATCAAGGTTACAGATCGACAGGTCT GGAGGTCAGCCCTGGAGAATGGCCGATACGAGCTGTTAAGTGAGGAAAGCAAGGAAAACGGAGTGAGCAA AGCTGTGAACGACGATGTGGAGGAGATGGAGATTGAACAAGCAAGGGTCATAGTAAGAGACAGACTTTTG GGGCTTAAAACAGAAACTCCAAGTACCGTCTCAACTAGTACCAGTACACCACAGTCAGTGAGCAATGTGG TTCATTATCTGGCTACAGCCCTCTTTCAGATAGAACAGGGCATTGAACGGCGTTTCCTCAAAGCTCCACT TGATGGCAGTGACAGCGGACGCTCTTATAAAACTGTCCTGGACCGTTGGAGAGAGTCTCTCCTTTCCTCT GCTAGTCTATCCCAAGTCTTTCTGCATTTATCAACCTTGGATCGCAGTGTGATGTGGTCTAAGTCCATAC TGAACGCACGTTGCAAGATCTGCCGGAAAAAGGGAGATGCTGAAAACATGGTGCTCTGTGACGGCTGTGA CCGAGGGCATCACACCTACTGCGTCCGACCAAAGCTCAAGACTGTTCCTGATGGAGATTGGTTTTGTCCA GAATGTCGACCAAAGCAACGTTCTAGAAGACTTTCCTCCAGACAGAGACCATCCTTGGAAAGTGATGAAG AAATGGAAGAGGGTATGGAAGGTGATGATGGTGAAGTTGATGACGATGATGAAGACGGTCAGAGTGAAGA GGAAGAATACGAGGTGGAGCAGGATGAGGAGGACTCTGACGAAGAGGAAGCCCTCAGCCCACCGAAACGA GGAAGACCCCAAGTTAGATTGCCAATTAAAACAAGAGGGAGATTTGGCCCTTCTTTCTCAAGTCGCAGCC AACGACAGGACCCTGGAAGATACCCTTCAAGGAGTCAGCAGAGCACGCCCAAAACCACTGCCTCTAAAAC TGCTGGCAGAAACCTAAGGAAAACCAGATCTGCTCCTCCCACAGAAACTCGGTCTCTAAGACTTGGCAGT CGCTCCACTCGCCACAGTCCTAACGCGCTGCAAGCAGATGTGTTTGTGGAACTGCTTAGTCCTCGTGGGA AGCGTAGAGTGAGGAAGAGTGCTGACAATACCCCAGAGCACAGCCCCAGCCTCACCAACCTCAGGGTCAG CACCTCAAGGCCAAGCGTACAGTTGATTCCTTTAAATTCTGCTGAAAGACTTTCTCTCCAGGATAGTGAA TCTAAGAGAAGAGGCAGGAAGAGACAATCTACAGAGTCATCTCCTGTACCACTGAATCGAAGAAGTTCTG GCCGGCAAGGAGGTGTTCATGAATTGTCTGCTTTTGAACAACTTGTTGTGGAATTGGTACGGCATGATGA TAGCTGGCCCTTCTTGAAACTTGTTTCTAAAATCCAGGTCCCAGACTACTATGACATCATTAAGAAGCCC ATTGCCTTAAATATAATTCGAGAAAAAGTAAATAAATGTGAATATAAATTAGCATCTGAGTTTATTGAAG ATATTGAGTTAATGTTTTCAAACTGTTTTGAATACAACCCGCGGAACACAAGTGAAGCAAAAGCTGGAAC TCGGCTCCAAGCATTTTTTCATATTCAAGCTCAAAAGCTTGGACTGCATGTCTCACCCAGTAATGTGGAC CAGGCTAGCACGCCACTAGCTGCAAAGAAGTCACGGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217655 representing NM_001170568 Red=Cloning site Green=Tags(s) MPLLHRKPFVRQKPPGDLRPDEEVFYCKVTNEIFRHYDDFFERTILCNSLVWSCAVTGRPGLTYQEALES ERKAKQDLQSFPEPLIIPVLYLTNLTHRSRLHEICDDIFAYIKDRYFVEETVEVIRNNGTRLQCRILEVL PPLHQNGFANGHMSSVDGETIVISDSDDSETQSSSFQHGKKKDAIDSLLFRYRVQPTKKEMYESAVVKAT QISRRKHLFSRDKLKLFLKQHCEPQDGVIKIKASSFSTYNIAEQDFSYFFPDDPPTFIFSPANRRRGRPP KRISFGQEDNIASKQTIARCRSKAIKERDKLLKQEEMRALAFEKAKLKREKADALEARKREREDREKKRE ELKKIVEEERLKKKEEKERLKIEREKEREKLREEKRKYMEYLKQWSKPREDMECDDLKELPEPTPVKTRL PPEVFGDALMVLEFLNAFGELFDLQDEFPEGVTLEVLEEALVGNDSEGPLCELLFFFLTAIFQAMAEEEE EVAKEQITDADTKDLTEALDEDADPTKSALSAVAALAAAWPQLHQGCSLKSLDLDSCTLSEILRLHILAS GADVTSANAKYRYQKRGGFDATDDACMELRLSNPSLVKKLSSTAVYDLTPGEKMKILHALCGKLLTLVST RDFIEDYVDVLRQAKQEFRELKAEQHRKEREATAARIRRRKEEKLKEQEQKMKEKQEKLKEDEQRTSAAD LGEEGREDFDTSADSKEIERKDLDPDVVTEDEDDLGSHKRSRRGKAGQTAVKQCIKQEEMNYCIKQEPLT ADAEEALRQEHQRKEKELLDKIQSAIACTNIFPLGRDRLYRRYWVFPSIPGLFIEEDYSGLTEDMLLPRP SSFHSNAQPHSPRVSIKTEESFMSESTSSLDQGPFDDSLLLPKPVHKPNRWCFYSSCEQLDQLIEALNSR GHRESALKETLLQEKSRICAQLAHFSEEKFHFSDKPQADSKPVSSRGRSAACDTSQMSAERQLELRLRDF LLDIEDRIYQGTLGAIKVTDRQVWRSALENGRYELLSEESKENGVSKAVNDDVEEMEIEQARVIVRDRLL GLKTETPSTVSTSTSTPQSVSNVVHYLATALFQIEQGIERRFLKAPLDGSDSGRSYKTVLDRWRESLLSS ASLSQVFLHLSTLDRSVMWSKSILNARCKICRKKGDAENMVLCDGCDRGHHTYCVRPKLKTVPDGDWFCP ECRPKQRSRRLSSRQRPSLESDEEMEEGMEGDDGEVDDDDEDGQSEEEEYEVEQDEEDSDEEEALSPPKR GRPQVRLPIKTRGRFGPSFSSRSQRQDPGRYPSRSQQSTPKTTASKTAGRNLRKTRSAPPTETRSLRLGS RSTRHSPNALQADVFVELLSPRGKRRVRKSADNTPEHSPSLTNLRVSTSRPSVQLIPLNSAERLSLQDSE SKRRGRKRQSTESSPVPLNRRSSGRQGGVHELSAFEQLVVELVRHDDSWPFLKLVSKIQVPDYYDIIKKP IALNIIREKVNKCEYKLASEFIEDIELMFSNCFEYNPRNTSEAKAGTRLQAFFHIQAQKLGLHVSPSNVD QASTPLAAKKSRI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001170568
ORF Size	4659 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170568.1, NP_001164039.1
RefSeq Size	5443 bp
RefSeq ORF	4662 bp
Locus ID	314126
MW	178.1 kDa

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