Cdk5rap2 (NM_173134) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217696 representing NM_173134 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGACTCAGGGATGGAAGAGGACGTCACCCTGCCTGGGACCCTCAGCGGCTGCAGTGGCCTTCACC CTGTTCTACCAAGTGATCTGGATGTCATCAGTGACACTACTGGCTTGGGAAATGGAGTGCTCCCAATCAT GTCAGAAGAAAAGGTCTCTCCCACCAGAGCCCGGAACATGAAGGACTTTGAAAACCAAATCACTGAGCTG AAGAAGGAGAACTTCAATCTAAAGCTCCGGATCTACTTCCTTGAGGAGAGGATACAGCAGGAGTTTGCTG GCCCCACAGAGCACATCTACAAGAAGAACATTGAACTCAAGGTGGAAGTGGAAAGTCTGAAGCGTGAGCT CCAGGAGAGAGATCAGTTGCTCGTCAAAGCCTCCAAAGCCGTGGAGAGCCTAGCAGAAGGGGGTGGTTCT GAAATCCAGCGGGTGAAAGAAGATGCAAGGAAGAAGGTGCAGCAGGTGGAAGAACTCCTAACTAAAAGGA TACATCTTCTAGAAGAAGATGTGAAAGCCGCCCAAGCAGAACTGGAGAAGGCCTTTGCAGGGACAGAAAC AGAGAAGGCTCTTCGGCTCAGCTTAGAAAGCAAGCTCTCGGCGATGAAGAAGATGCAGGAGGGGGACCTG GAGATGACTCTGGCTCTGGAAGAGAAGGACAGACTGATTGAGGAGTTGAAGCTGTCTTTGAAGAGCAAAG AAGCTTTAATTCAGTGCCTTAAAGAGGAAAAATCTCAGATGGCATCTCCTGACGAGAACGTGTCATCTGG AGAACTCCGAGGACTTTCTGCCACTCTAAGAGAAGAAAAAGAGAGAGACGCTGAGGAACGGCAGAAGGAG AGAAACCACTTTGAAGAGAGGATCCAGGCACTTCAGGAGGACCTGAGAGAGAAGGAAAGAGAAATTGCTA CAGAGAAGAAAAATAGCTTAAAGAGGGATAAAGCCATTCAGGGTCTAACCATGGCATTAAAATCAAAGGA AAAAGAGGTTGAAGAACTTAATTCCATAATCAAAGAACTCACTGCTGACTCCACCCAAAGTAGAGAGGCT CCCTTGAAAACCCAGGTCTCCGAATTCGAGGTAAGAGAGTCTGAGAACTGTGAGGCTGCGCTGGCAGAGA AGGAAGCCCTGTTGGCCAAGCTGCACTCGGAAAATGTCACAAAGAATACTGAGAACCATAGGTTGCTCAG GAATGTTAAAAAGGTCACCCAGGAGTTGAATGACCTGAAGAAGGAGAAGCTGAGACTAGAGCGGGACCTG GAGGAAGCCCACCGAGAAGGGAACAGAGGAGCCCGAACCATCCATGACCTTAGAAATGAAGTTGAGAAAC TACGCAAGGAAGTGTGTGAGAGAGAGAAGGCTGTGGAGAAGCATTACAAGAGTCTTCCAGGCGAAAGCAG TTCAAAGTTCCATTCTCAAGAACAAGTGGTCAAAGGTCTGACAGAAAGTGCCAGTCAGGAGGACCTGCTG CTGCAGAAATCCAATGAAAAAGATTTGGAAGCAATACAGCAGAACTGTTATTTAATGACTGCAGAGGAGC TCAAGTTTGGGAGCGATGGCTTAATAACAGAAAAGTGTTCTCAGCAGTCACCAGACAGCAAACTCATCTT CTCCAAGGAAAAGCAACAATCAGAGTATGAAGGGCTGACTGGGGACTTAAAGACAGAACAGAACGTCTAT GCCCATCTGGCAAAGAATCTGCAGGACACAGACAGCAAACTGCAGGCTGAATTGAAAAGGGTTCTTGCCC TCCGGAAGCAGCTGGAGCAGGATGTGCTTGCCTATCGGAATCTGCAGACGGCCCTGCAGGAGCAGCTCAG TGAAATTCGGAAGCGGGAAGAAGAGCCGTTTTCATTTTATAGCGATCAAACATCATATCTAAGTATTTGC CTTGAAGAGCACAGTCAGTTTCAATTGGAGCATTTTTCTCAAGAAGAAATCAAGAAAAAGGTCATTGACC TCATACAGCTTGTGAAGGACCTGCATGCAGACAACCAGCACCTGAAGAAAACCATTTTTGATATCTCTTG CATGGGTGTCCAGGGAAATGACAGACTTGAGTCAACAAAACAAGCAGAGCTTATGGCTAGCAAGGCGGAT GAGGACACACTCAAATTCAAAGCAGATGATGAGAATCATTTCCAGAGTGACCAGCATTTGGAGCAGAGTA GAGAGATCATGGAGGACTATGCTGAGGGTGGCGGCAAGGATGGATATGTAAGGCACATGGATTCCAATAT TTTAGACCATGATGGGGCCCACACACCTGATACCTCTGAAGACCATAGCCTAGAGGATGAGCTCCTGAGC CTACTCGCCACTTTCTTCAGTAAGAAGGCTACACCGTCGCTGGAGTCCAGGCCGGATCTGCTGAAAGCAC TTGGTGCCCTGCTTTTGGAACGGATATGCTTGGCAGAACAGGGAAGTCCTGGAGATCACTCTGATAGTAA AGCTGAGAAGGCACTTGAGCAAGTAGCTGTTCGGTTGAGAGATGAACTTGGCCATTCCTGCCTAGCCAAC TCATTCTCCAAGTCACACAGTGAGCTCAAGTCACCGCGGGGCACCTGGCTAGTAAAGACAGGTGATGAAG CCAAGGTGGAGCTGAAGAGTGTCTCAGTGCAGACCATGACCATAGAGGACAGCACCCGTGGATTCAAACC TGAGAGGAAGAGAGAAGCTTGGGCAGGGAAACCAGAAGAGGCCGTTTTCAGCACTGAGCTTGAGTCAGAG GCGTTGGGTGAGATGCCAGGGCTACAGGCCACTCACCTCTCATTCCCCAGTGCCATCGACAAGGATGACC AGAAGACAGGCTTGCTGATCCAATTAAAAACACCTGAGTTATTGGAAAATTTGTATAATCTTCCTGCCTC CCAAGAGGTGGTAGCTCAACTCCAGGGCCAGGTTCTAGAGCTGCAGAAAGAGCTGAAAGAGTATAAGATC CGCAACAAGCAACTCCTTGATAAGCTAATTCTGGCAGAGGCAATGATGGAGGGGATGGCAGTGCCCAACT CTACTCCGGTAAACGTGCCTGCAGCCCAGGCTGTTGTGAGAACAGCCTTCCAAGGCAAGCCAGGCGAGCA AGAAGGACATGAAACCACACATTCTGCCGGAAGAGACAAGGAAGTAGACAGTGACCAATATACAAGCTTT GAGATTGATTCTGAAATCTGCCCACCTGATGACCTTGCCTTATTACCAGCATGCAAAGAGAACCTTGAAG ACTTCTTAGGCCCACCCTCCATAGCCACTTACCTGGATTCCAAGAGTCAGCTCTCTGTTAAAGTCAGTGT GGTTGGAACTGACCAGTCTGAGAATATCAATCTCCCTGATGATACAGAAGCCTTAAAACAGAAAATCCAT GACTTGCAAACTGAGCTGGAAGGCTACCGGAACATCATTGTCCAGCTACAGAAGCATTCTCAGTGCAGTG AGGCTATTATTACAGTTTTGTGTGGGACAGAAGGGGCCCAAGATGGCCTGAACAAGCCTAAGGGCCACAT CGATGAAGAAGAGATGACATTTTCAAGTTTGCACCAAGTAAGATATGTGAAGCACATGAAAATCCTCCGT CCACTAACCCCAGAAATAATTGATGGCAAGATGCTAGAGAGCCTCAAGCAGCAGCTGGTAGAACAGGAGC AGGAGCTACAGAAGGAGCAGGATTTGAACTTGGAACTTTTCGGTGAAATCCATAACCTGCAGAACAAGTT CCGAGACCTCTCGCCATCAAGATACGATTCATTGGTTCAGTCCCAAGCAAGGGAGCTCTCTCTTCAGCGG CAGCAAATTAAGGATAGTCATGATATCTGTGTCGTTTGCCATCAACACATGAGCACCATGATCAAGGCAT TTGAGGAACTTCTGCAGGCCAGTGATGTGGACAGTTGTGTGGCTGAGGGCTTCCGCGAGCAGCTAACACA GTGTGCTGGACTGCTAGAGCAACTGGAGAAGCTGTTTCTCCATGGAAAGTCAGCCAGAGTGGAACCACAT ACCCAGACTGAACTGCTAAGGAGGTTAAGAACTGAAGAAGACAACTTACCTTACCAACATCTCCTGCCTG AATCTCCTGAGCCTTCTGCCTCTCATGCTCTGTCAGATGATGAAATGTCTGAGAAGTCCTTCCTGTCCAG AGAACCGAAGCCAGACAGTGAGACTGAGAAGTACCCAACAATAGCGAGCCGTTTTCCTCAAGACTTACTA ATGGAACACATACAGGAAATTCGAACTTTGAGAAAGCATTTGGAAGAATCTATTAAAACAAATGAGAAGC TCCGGAAACAGCTGGAACGGCAGGGATGTGAGACTGACCAAGGCTCTACAAATGTTTCTGCTTACAGCTC AGAACTGCATAATTCTCTGACATCAGAAATACAGTTCCTGAGGAAGCAGAATGAGGCCCTCAGTACGATG CTCGAGAAGGGGTCCAAAGAAAAACAGAAAGAGAATGAAAAATTGCGTGAGTCCCTGGCCAGGAAGACTG AGAGTCTTGAGCACCTTCAGCTAGAGTATGCCAGTGTAAGGGAAGAAAATGAAAGACTGCGGAGAGACAT CAGTGAGAAGGAGAGACAAAACCAGCAGTTGACTCAGGAGGTCTGCAGCAGCCTCCAAGAGCTGAGCAGG GTACAGGAAGAGGCAAAGTCAAGACAGCAGCTGCTCTTACAGAAAGACGAGCTGCTTCAGTCCCTCCAAA TGGAGCTTAAGGTGTATGAGAAGCTGGCCGAGGAGCATCAGAAGCTACAGCAAGATGTCAACAAATGTCC TGAGGCCAGCGATAATTCATTTGATCTATTTGAATCTACCCAGGCAATGGCACCTAAATCAGCTTCTGAG ACTCCTGTCCTTTCTGGAACTGATGTTGATTCCCTCTCCTGTGACAGTACCAGTTCTGCCACCAGCCCCT CTTGCATGCCCTGCCTGGTTGCTGGCCGCCACCTGTGGGCCAGCAAGAGTGGCCACCACATGCTGTGCCT GATTGAGGACTATGATGCCCTCTATAAGCAGATCAGCTGGGGCCAGACACTGCTTGCCAAGATGGATATT CAAACCCAAGAGGCTCTGAGCCCCACAAGTCAGAAGCTGGGACCAAAGGCTTCATTCTCTGTGCCTCTGA GCAAGTTTCTCTCCAGCATGAACACAGCCAAGCTGATCCTGGAAAAAGCCTCCAGGTTGCTGAAGCTCTT CTGGAGGGTCTCTGTCCCCACCAACGGCCAGTGTTCCCTTCACTGTGACCAGATTGGAGAAATGAAGGCA GAGATCACCAAACTACACAAAAAATTGTTTGAACAAGAAAAGAAGCTGCAGAACACAGCAAAACTTCTGC AGCAGAGCAAGCACCAGGAGAAAATCATCTTTGATCAGTTGGTCATCACCCACCAAGTCCTTCGGAAAGC CAGGGGAAACCTGGAGCTCAGGCCTAGAGCTGCCCATCCAGGAACATCCAGTCCCAGCAGACCGGGCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217696 representing NM_173134 Red=Cloning site Green=Tags(s) MMDSGMEEDVTLPGTLSGCSGLHPVLPSDLDVISDTTGLGNGVLPIMSEEKVSPTRARNMKDFENQITEL KKENFNLKLRIYFLEERIQQEFAGPTEHIYKKNIELKVEVESLKRELQERDQLLVKASKAVESLAEGGGS EIQRVKEDARKKVQQVEELLTKRIHLLEEDVKAAQAELEKAFAGTETEKALRLSLESKLSAMKKMQEGDL EMTLALEEKDRLIEELKLSLKSKEALIQCLKEEKSQMASPDENVSSGELRGLSATLREEKERDAEERQKE RNHFEERIQALQEDLREKEREIATEKKNSLKRDKAIQGLTMALKSKEKEVEELNSIIKELTADSTQSREA PLKTQVSEFEVRESENCEAALAEKEALLAKLHSENVTKNTENHRLLRNVKKVTQELNDLKKEKLRLERDL EEAHREGNRGARTIHDLRNEVEKLRKEVCEREKAVEKHYKSLPGESSSKFHSQEQVVKGLTESASQEDLL LQKSNEKDLEAIQQNCYLMTAEELKFGSDGLITEKCSQQSPDSKLIFSKEKQQSEYEGLTGDLKTEQNVY AHLAKNLQDTDSKLQAELKRVLALRKQLEQDVLAYRNLQTALQEQLSEIRKREEEPFSFYSDQTSYLSIC LEEHSQFQLEHFSQEEIKKKVIDLIQLVKDLHADNQHLKKTIFDISCMGVQGNDRLESTKQAELMASKAD EDTLKFKADDENHFQSDQHLEQSREIMEDYAEGGGKDGYVRHMDSNILDHDGAHTPDTSEDHSLEDELLS LLATFFSKKATPSLESRPDLLKALGALLLERICLAEQGSPGDHSDSKAEKALEQVAVRLRDELGHSCLAN SFSKSHSELKSPRGTWLVKTGDEAKVELKSVSVQTMTIEDSTRGFKPERKREAWAGKPEEAVFSTELESE ALGEMPGLQATHLSFPSAIDKDDQKTGLLIQLKTPELLENLYNLPASQEVVAQLQGQVLELQKELKEYKI RNKQLLDKLILAEAMMEGMAVPNSTPVNVPAAQAVVRTAFQGKPGEQEGHETTHSAGRDKEVDSDQYTSF EIDSEICPPDDLALLPACKENLEDFLGPPSIATYLDSKSQLSVKVSVVGTDQSENINLPDDTEALKQKIH DLQTELEGYRNIIVQLQKHSQCSEAIITVLCGTEGAQDGLNKPKGHIDEEEMTFSSLHQVRYVKHMKILR PLTPEIIDGKMLESLKQQLVEQEQELQKEQDLNLELFGEIHNLQNKFRDLSPSRYDSLVQSQARELSLQR QQIKDSHDICVVCHQHMSTMIKAFEELLQASDVDSCVAEGFREQLTQCAGLLEQLEKLFLHGKSARVEPH TQTELLRRLRTEEDNLPYQHLLPESPEPSASHALSDDEMSEKSFLSREPKPDSETEKYPTIASRFPQDLL MEHIQEIRTLRKHLEESIKTNEKLRKQLERQGCETDQGSTNVSAYSSELHNSLTSEIQFLRKQNEALSTM LEKGSKEKQKENEKLRESLARKTESLEHLQLEYASVREENERLRRDISEKERQNQQLTQEVCSSLQELSR VQEEAKSRQQLLLQKDELLQSLQMELKVYEKLAEEHQKLQQDVNKCPEASDNSFDLFESTQAMAPKSASE TPVLSGTDVDSLSCDSTSSATSPSCMPCLVAGRHLWASKSGHHMLCLIEDYDALYKQISWGQTLLAKMDI QTQEALSPTSQKLGPKASFSVPLSKFLSSMNTAKLILEKASRLLKLFWRVSVPTNGQCSLHCDQIGEMKA EITKLHKKLFEQEKKLQNTAKLLQQSKHQEKIIFDQLVITHQVLRKARGNLELRPRAAHPGTSSPSRPGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_173134
ORF Size	5460 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_173134.2, NP_775157.2
RefSeq Size	5654 bp
RefSeq ORF	5463 bp
Locus ID	286919
MW	206.4 kDa
Gene Summary	neuronal cyclin-dependent kinase 5 (Cdk5) activator binding protein [RGD, Feb 2006]

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