Kmt2e (NM_001100851) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mll5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217701 representing NM_001100851 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATAGCGATCCCATTGGGGGTTGATACAACAGAAACATCCTACTTGGAAATGGCTGCAGGCTCAG AACCAGAATCCGTAGAAGCTAGCCCTGTGGTAGTTGAGAAATCCAACAGTTATCCCCACCAGTTATATAC CAGCAGCTCCCATCATTCACACAGCTACATTGGTTTGCCCTATGCGGACCATAATTATGGTGCTCGTCCT CCTCCAACACCTCCAGCATCCCCGCCTCCATCAGGTCTCATTAGCAAAAACGAAGTAGGCATATTCACCA CTCCTCACTTTGACGAGACTTCCAGTGCTACTACAATCAGCACGTCTGAAGATGGGAGCTACGGCACTGA CGTGACCCGGTGCATATGTGGCTTTACACACGATGACGGCTACATGATCTGCTGTGACAAATGCAGTGTT TGGCAACATATTGACTGCATGGGGATTGACAGGCAGCATATTCCTGATACATATCTATGTGAACGTTGTC AGCCCAGGAGTTTGGATAAAGAGAGGGCAGTGCTACTGCAACGCCGGAAAAGAGAAAATATGTCAGATGG TGACACCAGTGCAACTGAAAGTGGCGATGAGGTCCCCGTGGAATTATACACTGCATTCCAGCACACTCCA ACTTCAATCACCTTAGCTGCTTCCAGAGCCCCCAAGGTCACTGATAAGAGGAGGAAAAAAAGTGGGGAGA AAGAACAAAACTTTCCAAAATGTAAAAAAGCATTTCGTGAAGGATCTAGGAAATCATCAAGGGTTAAGGG TTCAGCACCAGAAATTGATCCTTCGTCTGATGGTTCAAATTTTGTATGGGAGACAAAAATCAAAGCATGG ATGGATCGCTACGAAGAAGCAAATAATAACCAGTATAGCGAGGGCGTGCAGAGAGAGGCGCAAAGACTAG CGCAAAGACTCGGCAGTGGGAGTGACAGCAAAGACATGAGTAAACCAGAATTGAGTGTCAACAGCTCCCT CTTCAGACCTCCTGTAGAGAGCCATATACAGAAGAATAAAAAAATTCTGAAATCTGCCAAAGACTTGCCT CCTGATGCACTTATCATTGAATACAGAGGAAAGTTTATGCTGAGAGAACAGTTTGAAGCAAATGGATATT TCTTTAAAAGGCCATACCCTTTTGTTTTATTCTACTCCAAGTTCCATGGGCTAGAAATGTGTGTTGATGC AAGGACTTTTGGGAATGAGGCTCGATTCATCAGGCGTTCTTGTACACCGAATGCAGAGGTGAGGCATGAA ATTGAAGAGGGGACCATACATCTCTACATTTACTCTACACAGAGTATTCCAAAAGGGACTGAAATTACTA TTGCCTTTGATTTTGACTATGGGAATTGTAAATACAAGGTGGACTGTGCGTGCCTCAAGGAAAATCCAGA GTGCCCTGTTCTAAAGCGCAGCTCAGAATCTACAGAAAACATTAATAGTGGTTATGAGACCAGAAGAAAA AAAGGAAAAAAAGAGAAAGACATTTCAAAAGAAAAAGATATACAAAATCAAAATATTACTTTGGACTGTG AAGGAACAAACAACAAAATAAGGAGTCCAGAAACAAAACAGAGAAAGCTTTCTCCATTAAGGCTGTCAGT GTCCAACAACCAGGAACCAGATTTTATTGATGATATGGAAGAAAAAACTCCTATTAGCACTGAAGTTGAA ATGGAATCAGAGGAGCAGATTGCGGAGAGGAGGAGGAAGATGACAAGAGAAGAAAGAAAAATGGAAGCAA TTCTGCAAGCTTTTGCCAGACTTGAAAAGAGAGAGAAGAGACGAGAACAAGCTTTGGAAAGGATCAGCAC AGCCAAGACGGAAGTCAGGCCCGAGTGTAAGGAGTCGCAGGTCATCTCTGACGCTGACGTGGTCCAGGAA CAAGTAAAGGAAGAAACTGCTATCAAGCCAACTGCTGCCAAAGTGAACAGAACTAAGCAGAGAAAAAGCT TTTCTCGTAGTAGGACTCATATTGGACAGCAGCGTCGGAGACACAGAACCGTCAGCATGTGTTCAGACAT ACAGCCATCCTCTCCCGACGTTGAAGTTATGTCACAACAGAATGATATTGAAAACACTGTGCTTGCTATA GAACCAGAAACTGAAGCTGCAGTAGCAGAGATAATCACTGAAGTGGAAGCTCCAGCACTTAATAAATGTC CCACAAAGTACCCCAAAACAAAGAAGCACTTGGTCAATGAATGGTTAAGTGAGAAGAATGAGAAGACAGG AAAACCTTCAGACAGCCTTTCAGAAAGGCCTCTGCGCATAACTACAGATCCAGAGGTGTTAGCTACACAG CTCAATTCTTTGCCAGGTCTCACTTACAGCCCCCATGTATACTCTACTCCTAAGCATTACATTAGATTCA CATCACCATTCCTTTCGGAAAAAAAGAGAAGAAAAGAAACTACTGAAAACATTTCTGGCTCATGCAAAAA GCGATGGTTGAAGCAAGCCCTGGAAGAAGAAAATTCAACAGTTTTACACAGATATCATTCACCGTGTCAG GAAAGGCCCAGGAGCCCTACAGTTAATGGTGAAAGTAAAAGTCCCCTGCTGCTGAGCGACAGCTGCTCCC TCCCAGATTTAACCACACCACTAAAAAAGCGAAGACTTTACCAATTGTTAGACTCGGCTTACTCAGAAAG CTCCACCCCTACTCCTTCACCATACGCCACCCCAACTCACACGGACATCACTCCTACCGACCCAGCATTT GCTACTCCCCCACGGATCAAATCAGATGATGAAACTTACAGAAATAGTTATAAACCCATATATTCACCAG TGACCCCAGTGACCCCTGGCACACCAGGAAACACCATGCACTTTGAGAATATTTCTTCCCCAGAAAGTTC TCCAGAAATAAAGAGATGCACTTACAATCAAGAGGGATATGACCGACCTTCAACCATGCTAACGCTGGGG CCTTTTAGAAATTCCAACCTAAATGAGCTAGGCCTGCAAGAAATAAAGACCATTGGTTACACCAGCCCCA GGAGCAGGACTGAGGTTAATAGGCCTTGCCCAGGAGAAAAGGAGTCTGTGTCAGACCTTCAACTGGGACT CGATGCAGTCGAGCCAGCTGCCTTACAGAAAACCATGGAAACCCCAGCCCATGACAGGACTGAGCCCAGC AGCCAACTGGATTCGACCCACTCTGGACGAGGCACAATGTATTCATCCTGGGTAAAGAGTCCTGACAGAA CAGGAGTTAACTTCTCAGTAAACTCCAACTTGAGGGACTTAACACCCTCTCATCAGTTGGAGATTGGAGG CGGCTTCCGAATCAGTGAGTCCAAGTGCCTGACGCAGGATGATGCTAGAGGCATGTTTATGGAGACAGCT GTCTTTTGTCCCTCTGAAGATGGGCTTGTCTCTGGTTTTGGACGGACTGTTAATGACAGTTTGATCGACG GGAGCTGCACGCCCCAGAATCCACCACAAAAGAAAAAGGTCTCTCTACTAGAATACCGTAAGAGACAACG TGAAGCTAGGAAGAGTGGTTCTAAGCCAGAGAACCTTGCTTTGATTAGTGTGTCACCTCACCCAAGTGGG ACTCTAAGCAGCAGTGGCGATGGCTGTGTCCACAGCAGTGAGAACGGGGAGCAGCCAGAAAACCGGGCTA GCTTGCCTTTAGCACCGCCAGCTGCCGCCTGCACCGCCTCCTGTGAAGAAGGCAGCAGTGACTGTCCTGT CAGAGACGCCAACCCCACTGAGAAGACAGACCCAGAAGTTCAGTGGACTGCTTCCACCTCAGTGGAGCAA GTGAGAGAGAGAAGCTATCAGAGAGCGTTATTGCTCAGTGACCACCGCAAAGACAAAGACGGCGGGGGAG AGTCACCATGTGTCTCATGTTCACCGAGTCATGTTCAGTCTTCACCTTCATCTCATTCAAACCACATTCC CCAGGTGCCCGCTCAGAGCCTAGCCCCTCCTTTGACTGAACCTATGGCAGACCTTGATCCTGAAGGTACA GAAGCCACAAGTACAAGTGAATGTCCATCCCCTGACACTTCTCAGAGCCCTTCTAAAACAAGCAAGCCTG GTTCACCAGGGCCAATCAATCCTGCTCAGTCACATGGGAAAATGCTCACAAAACCAGATTCCCACTGGGA GGCAACTGCCACTGTATCAGAAGCTGAGAGCAGTGTTCACCTAAAACCAGAGCTTCAGCAAAAACAGCTG TCAAGTAACACCCCAGCACTTTCAAAGAACCATCCTCCTCAGGCCCACGTTCGAAATGCAAACGATCAAC TTCCGCAAAAGCTGCCTTCTGCACCAACAAAATTGCACTGTCCTCCGTCCCCTCACACAGAAAACCCTCC CCAGTCCTCCACGCCTCACACGCCTGTGCAGCATGGTTACCTCTCACCAAAGCCTCCTTCACAGCACCTA GGATCTCCCTTCAGGCCTCACCATTCACAGTCACCTCAAGTCGGAACTCCTCAACGCGAGACTCCGAGAA GTTTGTATCCAGCGGCACAGAACCTTCAAGCTAATGCTCAGCAGGCACCTTGTGGAGCGTTATTTACCCA GACACCCTCAGGCCAGTCCTCGGCAGCATACAGTCCGTTCAACCAACAGGCTCTGAGCAGCCCGGCCCCG CCCCCCCCACCCCCTCCGCCTCCTTCAGCATACTATCAAAACCAGCAGCCCTCTGCAAACTTTCAGAACT ACAATCAGCTAAAAGGTAGTCTTTCCCAACAAACTGTGTTTACATCTGGACCAAATCAAGCACTTCCTGG CACCACAAGCCAGCCATCCGTTCCTGGACACCATGTTACTCCGGGCCATTTTCTGCCGTCTCAAAACCCT ACCATTCACCATCAAACTGCTGCTGCTGTGGTCCCACCCCCGCCTCCACCACCGCCTGCTCCAGGACCTC ACCTCGTACAACAGCCAAACTCACATCAGCAGCACTCTGTAGCACATGTAGTTGGGACAGTTCATGCTGT CACCCCTGGGTCACACATTCATTCTCAAACTGTTGGACACCATTTACCACCACCTCCTCCACCTCCTGGT CCTGCCCCACATCACCATCCTCCACCCCATCCTTCCACAGGACTGCAAAGTCTGCAAGCACAACACCAGC ATGTAGTAAACTCAGCACCCCCACCGCCCCCTCCTCCTCCTCCTCCAGCCAGTGTTCTGGTTTCTGGGCA TCATTCAGCATCAGGTCAAGCCTTACATCACCCACCTCATCAAGGACCTCCACTTTTTCCTGCGAGTGTT CATCCGGCTGTACCGCCGTACCCCTCACAAGCCACACATCACACCACTTTGGGACCGGGACCCCAACACC AGCCTTCCGGAACAGGGCCACATTGTCCATTACCAGTTCCAGGTCCTCATCTCCAGCCCCAAGGACCAAA CAGTATTCCAACACCTACTGCTTCAGGGTTCTGTCCTCATCCTCACCCTGGCTCTGTGGCCCTGCCACAT GGGGTGCAAGGACCTCAGCAGGCATCGCCAGTGCCTGGACAGATTCCAATTCACAGAGCACAGGTGCCAC CAACATTTCAAAACAATTACCACGGTTCAGGGTGGCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217701 representing NM_001100851 Red=Cloning site Green=Tags(s) MSIAIPLGVDTTETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARP PPTPPASPPPSGLISKNEVGIFTTPHFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSV WQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTP TSITLAASRAPKVTDKRRKKSGEKEQNFPKCKKAFREGSRKSSRVKGSAPEIDPSSDGSNFVWETKIKAW MDRYEEANNNQYSEGVQREAQRLAQRLGSGSDSKDMSKPELSVNSSLFRPPVESHIQKNKKILKSAKDLP PDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHE IEEGTIHLYIYSTQSIPKGTEITIAFDFDYGNCKYKVDCACLKENPECPVLKRSSESTENINSGYETRRK KGKKEKDISKEKDIQNQNITLDCEGTNNKIRSPETKQRKLSPLRLSVSNNQEPDFIDDMEEKTPISTEVE MESEEQIAERRRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVRPECKESQVISDADVVQE QVKEETAIKPTAAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIQPSSPDVEVMSQQNDIENTVLAI EPETEAAVAEIITEVEAPALNKCPTKYPKTKKHLVNEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQ LNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKKRRKETTENISGSCKKRWLKQALEEENSTVLHRYHSPCQ ERPRSPTVNGESKSPLLLSDSCSLPDLTTPLKKRRLYQLLDSAYSESSTPTPSPYATPTHTDITPTDPAF ATPPRIKSDDETYRNSYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRCTYNQEGYDRPSTMLTLG PFRNSNLNELGLQEIKTIGYTSPRSRTEVNRPCPGEKESVSDLQLGLDAVEPAALQKTMETPAHDRTEPS SQLDSTHSGRGTMYSSWVKSPDRTGVNFSVNSNLRDLTPSHQLEIGGGFRISESKCLTQDDARGMFMETA VFCPSEDGLVSGFGRTVNDSLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKPENLALISVSPHPSG TLSSSGDGCVHSSENGEQPENRASLPLAPPAAACTASCEEGSSDCPVRDANPTEKTDPEVQWTASTSVEQ VRERSYQRALLLSDHRKDKDGGGESPCVSCSPSHVQSSPSSHSNHIPQVPAQSLAPPLTEPMADLDPEGT EATSTSECPSPDTSQSPSKTSKPGSPGPINPAQSHGKMLTKPDSHWEATATVSEAESSVHLKPELQQKQL SSNTPALSKNHPPQAHVRNANDQLPQKLPSAPTKLHCPPSPHTENPPQSSTPHTPVQHGYLSPKPPSQHL GSPFRPHHSQSPQVGTPQRETPRSLYPAAQNLQANAQQAPCGALFTQTPSGQSSAAYSPFNQQALSSPAP PPPPPPPPSAYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGTTSQPSVPGHHVTPGHFLPSQNP TIHHQTAAAVVPPPPPPPPAPGPHLVQQPNSHQQHSVAHVVGTVHAVTPGSHIHSQTVGHHLPPPPPPPG PAPHHHPPPHPSTGLQSLQAQHQHVVNSAPPPPPPPPPPASVLVSGHHSASGQALHHPPHQGPPLFPASV HPAVPPYPSQATHHTTLGPGPQHQPSGTGPHCPLPVPGPHLQPQGPNSIPTPTASGFCPHPHPGSVALPH GVQGPQQASPVPGQIPIHRAQVPPTFQNNYHGSGWH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100851
ORF Size	5568 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100851.1, NP_001094321.1
RefSeq Size	7209 bp
RefSeq ORF	5571 bp
Locus ID	311968
MW	203.5 kDa

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