Ncoa6 (NM_001276714) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Aib3; NRC; PRIP; RAP250; Trbp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217732 representing NM_001276714 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTTTGGATGACCTTCCAAACTTTGAAGACATCTATACTTCCTTGTGTTCATCAACAATGGGAGACT CAGAGGTGGAGTTTGACTCTGGACTAGAAGATGATGACACAAAAAGTGATAGTATTTTGGAGGATTCTAC AATTTTTGTAGCCTTCAAAGGAAATATAGATGATAAAGACTTCAAATGGAAACTGGATACAATCTTACAG AGTGTGCCCGGCTTGTTACACATGGAATCCAGCAAGCTGAAGGTACAGAAAGTGGAGCCCTGGAACAGCG TGCGAGTCACATTCAACATCCCCCGGGAAGCAGCAGAGCGGTTACGGATCCTGGCTCAGAGCAACAACCA GCAGCTTCGGGATCTGGGGATTCTCTCCGTTCAGATTGAAGGGGAAGGTGCTATCAACCTGGCTTTGGGT CAGAACCGAAGCCAAGACGTGAGAATGAATGGACCCGTGGTATCTGGAAATTCCGTTAGGATGGAGGCAG GATTTCCCATGGCAAGTGGTCCAGGACTAATAAGAATGACAAGCCCTGCCACTGTTATGATGCCCCAGGG CGGGAACGCATCATCTTCCATGATGGCACCAGGCCCCAATCCAGAACTGCAACCCAGGACTCCTCGCCCT GCTTCTCAGTCAGATGCGATGGATCCACTTCTCTCTGGACTCCATATACAGCAACAGAGTCATCCCTCAG GATCTTTACCTCCAGCGCATCACCCAATGCAACCTGTTCCTGTGAACAGACAAATGAACCCAGCTAATTT TCCCCAGCTGCAGCAACAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAA CAGTTGCAGACTAGACCTTTACAACAACATCAGCAGCAACAGCCACAGGGGATTCGACCACAGTTTACTG CTCCAACTCAGGTGCCTGTTCCTCCAGGCTGGAACCAGCTGCCTTCTGGAGCCTTACAGCCTCCACCAGC CCAGGGATCTCTGGGCCCAATGACTACAAATCAAGGGTGGAAGAAGGCTCCCTTGCCTAGCCCAATGCAA GCGCAACTTCAGGCAAGACCTTCCTTAGCCACGGTACAGACACCTTCTCACCCTCCCCCTCCTTATCCTT TTGGCAGCCAACAAGCCTCACAAGCCCATACAAACTTTCCTCAAATGAGCAACCCAGGCCAGTTCACAGC TCCTCAGATGAAGAGCTTGCAGGGAGGACCCTCCAGGGTCCCAACCCCCCTGCAACAGCCCCACCTCACC AACAAGTCTCCTGCCTCCTCACCCTCCTCCTTCCAGCAGGGATCCCCTGCATCCTCCCCAACGGTTAACC AAACTCAGCAGCAGATGGGACCAAGGCCACCTCAAAATAACCCACTTTCCCAGGGATTTCAGCAGCCTGT CAGCTCTCCGGGTCGGAATCCTATGGTTCAACAGGGAAATGTGCCACCTAACTTCATGGTGATGCAGCAG CAGCCACCAAGCCAGGGGCCACCGAGTTTACACCCAGGCCTAGGAGGAATGCCTAAACGCCTCCCACCTG GCTTCTCAGCAGGACAGGCCAATCCCAACTTTATGCAAGGTCAGGTGCCTTCCACCACAGCAGCCACCCC TGGGAATTCAGGAGCCCTTCAGCTGCAAGCAAATCAAAGTGTCCAGCATGCAGGTGGTCAAGGAGCTGGT CCTCCTCAAAACCAGATGCAGGTGTCTCACGGGCCACCAAATATGATGCAACCCAGCCTCATGGGAATTC ATGGCAACATAAACAACCAGCAGGCTGGTAGCTCTGGGGTTCCTCAGGTGACCCTGGGCAGCATGCAAGG CCAGCCCCAGCAGGGCCCACCATCTCAGCTAATGGGCATGCACCAACAGATTGTGCCCTCACAGGGCCAA ATGGCCCAGCAGCAAGGAACTTTGAACCCTCAAAACCCTATGATCCTTTCAAGGGCCCAGCTTATGCCAC AGGGCCAGATGATGGTGAACGCTCAGAACCAAAATCTTGGACCTTCACCCCAAAGGATGACCCCACCCAA GCAGATGCTTCCCCAGCAGGGCCCACAAATGATGGCGCCACATAACCAGATGATGGGGCCTCAGGGGCAA GTGTTGCTCCAGCAGAATCCAATGATAGAGCAAATAATGACCAATCAGATGCAGGGGAATAAGGCGCAAT TTAATTCTCAGAACCAATCCAACGTCATGCCGGGACCAGCACAGATAATGAGGGGACCAACTCCTAACAT GCAAGGAAACATGGTGCAATTCACAGGACAGATGTCAGGACAGATGCTGCCTCAGCAAGGGCCTGTGAGC AACAGTCCATCTCAGGTTATGGGGATTCAGGGGCAGGTTCTGCGGCCACCAGGACCCAGCCCACACATGG CCCAGCAACATACTGATCCTGCTACTACAGCAAATAATGATGTCAACTTGTCTCAGATGATGCCTGATGT TAGCATGCAACAAACCAGCATGGTCCCTCCACATGTGCAGAGCATGCAGGGAAACAGTGCTTCGGGAAGC CACTTCTCAGGCCATGGAGTGTCTTTCAATGCACCATTCGGTGGTGCACCCAATGGAACTCAGATGTCTT GTGGTCAAAATCCAGGCTTTCCAGTAAATAAGGATGTAACGTTAACAAGCCCATTGTTGGTCAACTTACT GCAAAGTGACATTTCAGCAGGTCATTTTGGCGTAAACAATAAACAAAATAATACCAACGCGAATAAACAG AAGAAGAAGAAACCACCGCGGAAGAAGAAAAATTGTCACCAGGATCTAAACACCCCAGATAGTCGTCCAG CTGGTCTAGAGGAAGTTGATCAACAGTCATTACCTGGAGAACAAGGAATCAATTTGGACAACACAGGCCC TAAACTGCCAGACTTTTCAAACCGGCCACCAGGTTATCCTACACAACCAGTTGAACAGAGGCCACTGCAG CAGATGCCTCCTCAACTCATGCAGCATGTGGCACCCCCACCACAGCCACCACAGCAGCAGCCACAACCAC AACTGCCTCAACAGCAGCCACAGCCGCAGCCACCACCACCTAGTCAGCCACAGTCGCAGCAGCAGCAGCA GCAGATGATGATGATGCTCATGATGCAGCAAGATCCCAAATCCATTAGGCTTCCGGTCTCCCAAAATGTC CATCCTCCACGGGGTCCTCTGAACCCAGACTCCCAAAGAGTGCCCATGCAACAGAGTGGCAATGTGCCTG TCATGGTTAGTTTGCAAGGACCTGCCTCTGTGCCACCGTCACCTGATAAACAAAGGATGCCAATGCCTGT GAATACTCCGCTGGGAAGTAATTCAAGAAAGATGGTATACCAGGAGAGCCCACAGAATTCCAGCTCACCA CTAGGAGAGATGCCCTCACTTCCTGAAGCTGGTGGCAGTGAAGTACCATCTGTTTCAGGAGGCCCAAGTA ACATGCCCTCGCATTTAGTAGTTTCTCAGAATCAATTAATGATGACAGGACCCAAACCTGGACCATCTCC CCTTTCAGCAACTCAAGGTGCAACTCCCCAGCAGCCTCCTGTAAACTCCTTGCCTAGTTCCCATGGCCAC CACTTTCCAAATGTGGCTGCACCAACCCAAACATCTAGGCCTAAAACACCAAACAGAGCCAGCCCCAGAC CCTATTATCCTCAGACGCCCAACAACCGCCCTCCTAGCACAGAACCTTCAGAAATCAGTCTCTCTCCAGA AAGACTCAATGCTTCCATAGCAGGACTCTTCCCTCCACAGATTAATATTCCTTTACCTCCCAGGCCAAAC TTAAATAGGGGCTTTGATCAACAGGGCTTAAATCCAACAACTCTGAAGGCCATTGGGCAAGCACCTTCAA ATCTCACTGTAAGTAATCCTCCTAACTTTGCTGCCCCACAAGCTCATAAATTAGATTCTGTGGTGGTGAG TTCTGGAAAACAGTCTAATCCTGGAACAACAAAACGGGCAAGTCCAAGCAACAGTCGCAGGTCTAGTCCG GGGTCCAGTAGAAAGACTACCCCAAGTCCTGGAAGGCAAAATTCAAAAGCCCCTAAACTTACTCTGGCTT CTCAAACAAGCACAACCCTGTTGCAGAACATGGAGCTGCCTAGAAATGTGTTGGTTGGTCCCACTCCACT TGCCAATCCCCCTTTATCTGGAAGCTTTCCTAACAATAATGGGCTTAATTCCCAGAATCCCACCGTGCCT GCGCCTGCAGTGGGGACTGTTGTTGAGGATAACAAAGAGAGCTTGAATGTTCCTCAGGACAGTGATTGCC AGAATTCCCAGGGGAGGAAGGAACAGGTAAACACTGAGCTGAAAGCAGTCCCTATCCAAGAAGCTAAAAT GGTTGTCCCCGAAGATCAGTCCAAAAAGGATGGGCAACCTTTGGATCCTAACAAACTCCCCAGTGTAGAA GAGAACAAAACTTTGATGTCTCCTGCCATGAGAGAAGCACCAACATCACTAAGCCAACTTCTCGACAACT CTGGAGCTCCTAATGTGACCATTAAACCCCCTGGGCTTACAGATCTGGAAGTAACACCTCCAGCAGTTTC AGGAGAGGACCTCAAAAAAGCATCTGTCATTCCCACACTGCAGGATCCGTCTTCTAAAGAACCCTCTAAT TCTTTAAACTTACCTCACAGTAACGAGCCGTGTTCAACCCTTGCGCATCCAGAATTGAGTGAGGTCAGCT CAAACATTGCACCAAGCATCCCTCCAGTAATGTCAAGACCTGTCAGCTCTTCCTCCATTTCTACTCCCTT ACCCCCAAACCAAATAACTGTATTTGTTACTTCCAACCCCATAACCACTTCATCTAACACATCAGCAGCC CTGCCAACTCACTTGCAGTCTGCATTGATGTCGACAGTCGTCACGATGCCCAACGTGGGTAACAAAGTTA TGGTTTCTGAGGGACAGTCAGCTGCTCAATCTAATGCCCGGCCTCAGTTCATTACACCTGTCTTTATCAA TTCATCTTCAATAATTCAGGTTATGAAAGGATCACAGCCAAGCACAATCCCTGCAACCCCATTGACAACC AACAGTGGCCTGATGCCTCCCTCTGTCGCAGTTGTTGGACCTTTACACATACCTCAGAACATAAAATTTT CTTCAGCTCCTGTAACACCTAATGTCCCCTCCAGTAGTCCTGCTCCAAATATACAGACAGGTCGGCCATT GGTCCTTAGCTCACGAGCCACTCCTGTTCCGCTGCCTTCCCCTCCTTGTACATCCTCTCCAGTCGTCGCT CCTAATCCTTCTGTCCAGCAAGTAAAAGAATTAAATCCAGATGAGGCTAGTCCTCAGACGAACACCTCAG CAGACCAGAGCACTCTGCCTTCTTCACAACCAACCACAGTAGTTTCTCCCCTTTTGGCCAATAGTCCAGG CTCCTCTGCCAATCGGCGAAGCCCAGTCTCATCCAGTAAGGGCAAAGGAAAAGTGGACAAAATTGGCCAG ATTTTGCTGACCAAAGCTTGTAAGAAAGTTACAGGCTCTCTGGAGAAAGGGGAAGAACAGTATGGTGCAG ATGGAGAAACTGAAGGCCCAGGGCTAGAGACCACAACTCCTGGGCTAATGGGAACAGAGCAGTGCTCCAC AGAGCTGGACAGTAAAACCCCAACACCCTCAGCACCCACTCTCCTAAAAATGACCTCTAGCCCCATGGGC CCAAGCTCCACCTCAACAGGACCCATCTTACCTGGCGGTGCTCTTCCCACCAGTGTACGCTCTGTAGTAA CCACATTGGTACCCTCTGAGCTCATCTCCACAGCGCCAACCACAAAAGGCAATCATGGTGGGATAACATC TGAGCCACTTGCAGGTGGCCTAGTGGAGGAGAAGGTGGGATCCCATCCAGAGCTTCTACCCAGCATAGCC CCTTCACAGACTTTAGTCCCAAAGGAATCTCCAGCCACAGCACTGCAGGGCTCTGTTGGCAGACCAGAAC TTGAGGCAAATGCTGCCATAGCCTCTGGACAAAGCAGTGAGCCAAAAGAGACAGTTGAGAAGTCCAAAAC CCCCAGCCGAAGAAACTCTCGAACTGAGGAGCCAACTATGGCTTCTGAGAATGTGGAAAATGGACATCGA AAAAGATCTTCTCGACCTGCTTCAGCCTCCAGCTCTACTAAAGATATAACTGGTGCAGTGCAATCTAAGC GAAGAAAATCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217732 representing NM_001276714 Red=Cloning site Green=Tags(s) MVLDDLPNFEDIYTSLCSSTMGDSEVEFDSGLEDDDTKSDSILEDSTIFVAFKGNIDDKDFKWKLDTILQ SVPGLLHMESSKLKVQKVEPWNSVRVTFNIPREAAERLRILAQSNNQQLRDLGILSVQIEGEGAINLALG QNRSQDVRMNGPVVSGNSVRMEAGFPMASGPGLIRMTSPATVMMPQGGNASSSMMAPGPNPELQPRTPRP ASQSDAMDPLLSGLHIQQQSHPSGSLPPAHHPMQPVPVNRQMNPANFPQLQQQQQQQQQQQQQQQQQQQQ QLQTRPLQQHQQQQPQGIRPQFTAPTQVPVPPGWNQLPSGALQPPPAQGSLGPMTTNQGWKKAPLPSPMQ AQLQARPSLATVQTPSHPPPPYPFGSQQASQAHTNFPQMSNPGQFTAPQMKSLQGGPSRVPTPLQQPHLT NKSPASSPSSFQQGSPASSPTVNQTQQQMGPRPPQNNPLSQGFQQPVSSPGRNPMVQQGNVPPNFMVMQQ QPPSQGPPSLHPGLGGMPKRLPPGFSAGQANPNFMQGQVPSTTAATPGNSGALQLQANQSVQHAGGQGAG PPQNQMQVSHGPPNMMQPSLMGIHGNINNQQAGSSGVPQVTLGSMQGQPQQGPPSQLMGMHQQIVPSQGQ MAQQQGTLNPQNPMILSRAQLMPQGQMMVNAQNQNLGPSPQRMTPPKQMLPQQGPQMMAPHNQMMGPQGQ VLLQQNPMIEQIMTNQMQGNKAQFNSQNQSNVMPGPAQIMRGPTPNMQGNMVQFTGQMSGQMLPQQGPVS NSPSQVMGIQGQVLRPPGPSPHMAQQHTDPATTANNDVNLSQMMPDVSMQQTSMVPPHVQSMQGNSASGS HFSGHGVSFNAPFGGAPNGTQMSCGQNPGFPVNKDVTLTSPLLVNLLQSDISAGHFGVNNKQNNTNANKQ KKKKPPRKKKNCHQDLNTPDSRPAGLEEVDQQSLPGEQGINLDNTGPKLPDFSNRPPGYPTQPVEQRPLQ QMPPQLMQHVAPPPQPPQQQPQPQLPQQQPQPQPPPPSQPQSQQQQQQMMMMLMMQQDPKSIRLPVSQNV HPPRGPLNPDSQRVPMQQSGNVPVMVSLQGPASVPPSPDKQRMPMPVNTPLGSNSRKMVYQESPQNSSSP LGEMPSLPEAGGSEVPSVSGGPSNMPSHLVVSQNQLMMTGPKPGPSPLSATQGATPQQPPVNSLPSSHGH HFPNVAAPTQTSRPKTPNRASPRPYYPQTPNNRPPSTEPSEISLSPERLNASIAGLFPPQINIPLPPRPN LNRGFDQQGLNPTTLKAIGQAPSNLTVSNPPNFAAPQAHKLDSVVVSSGKQSNPGTTKRASPSNSRRSSP GSSRKTTPSPGRQNSKAPKLTLASQTSTTLLQNMELPRNVLVGPTPLANPPLSGSFPNNNGLNSQNPTVP APAVGTVVEDNKESLNVPQDSDCQNSQGRKEQVNTELKAVPIQEAKMVVPEDQSKKDGQPLDPNKLPSVE ENKTLMSPAMREAPTSLSQLLDNSGAPNVTIKPPGLTDLEVTPPAVSGEDLKKASVIPTLQDPSSKEPSN SLNLPHSNEPCSTLAHPELSEVSSNIAPSIPPVMSRPVSSSSISTPLPPNQITVFVTSNPITTSSNTSAA LPTHLQSALMSTVVTMPNVGNKVMVSEGQSAAQSNARPQFITPVFINSSSIIQVMKGSQPSTIPATPLTT NSGLMPPSVAVVGPLHIPQNIKFSSAPVTPNVPSSSPAPNIQTGRPLVLSSRATPVPLPSPPCTSSPVVA PNPSVQQVKELNPDEASPQTNTSADQSTLPSSQPTTVVSPLLANSPGSSANRRSPVSSSKGKGKVDKIGQ ILLTKACKKVTGSLEKGEEQYGADGETEGPGLETTTPGLMGTEQCSTELDSKTPTPSAPTLLKMTSSPMG PSSTSTGPILPGGALPTSVRSVVTTLVPSELISTAPTTKGNHGGITSEPLAGGLVEEKVGSHPELLPSIA PSQTLVPKESPATALQGSVGRPELEANAAIASGQSSEPKETVEKSKTPSRRNSRTEEPTMASENVENGHR KRSSRPASASSSTKDITGAVQSKRRKSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276714
ORF Size	6174 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276714.1, NP_001263643.1
RefSeq Size	6694 bp
RefSeq ORF	6177 bp
Locus ID	116464
MW	218.2 kDa
Gene Summary	interacts with nuclear hormone receptors to activate gene transcription [RGD, Feb 2006]

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