Wnk2 (NM_001191556) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1307284
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217733 representing NM_001191556 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGCGATGGCGGCCGCCGAGACGCCCCCGGCGCGTTGATGGAGGCCGGGCGCGGCACGGGGTCGG CGGGCATGGCGGAGCCGCGAGCGAGGGCGGCGCGGCTCGGGCCCCAGCGCTTCCTGCGGCGCAGCGTGGT GGAGTCGGACCAGGAGGAGCCGCCGGGCCTGGAGGCGGCCGAGACGCCGAGCGCGCAGCCCCCACAGCCC CTGCAGCGCAGGGTGCTTCTACTCTGCAAGACGCGCCGCCTCATCGCGGAGCGCGCCCGGGGCCGCCCCG CCGCGCCCGCGCCTGCCGCGCCCGCTGCGCCTCCTGGCTCCCCGAGTGCCCCCTCGGACCCTGGCCCGGA GCGAGCGGGCACGCAGGAGCCCAGCCCAGATCCCACCACCGCGTCAGCTGCAGCCACCCCGGTCCCCGAC GGCGGCCCGCGGCAGGAGGAGGCGCCCGCCCCAACCCAGGAGGACACTGGAGCTACCGAGGCAAAGCCGG AGCCCGGGCGCGCACGCAAGGATGAACCTGAGGAGGAGGAGGACGATGAGGACGACCTCAAGGCTGTGGC TACCTCTTTGGACGGCCGCTTCCTCAAGTTCGACATCGAGCTGGGCCGGGGCTCCTTCAAGACGGTCTAC AAGGGGCTGGACACGGAGACCTGGGTGGAGGTAGCATGGTGTGAACTGCAGGACCGGAAGCTCACCAAAC TCGAACGGCAGCGGTTCAAAGAGGAGGCAGAGATGCTGAAAGGTCTACAGCACCCCAACATTGTGCGCTT CTATGACTTCTGGGAGTCCAGTGCCAAGGGCAAACGATGTATTGTGCTGGTGACGGAGCTGATGACCTCA GGGACATTGAAGACGTACCTGAAGCGGTTCAAGGTGATGAAGCCCAAGGTACTGAGAAGCTGGTGCCGGC AGATCCTGAAGGGCCTGTTGTTTCTGCACACAAGGACTCCCCCCATCATCCACCGAGACCTCAAGTGTGA CAACATCTTCATCACCGGGCCCACCGGCTCCGTGAAGATTGGCGACTTGGGTCTGGCCACCCTGAAAAGA GCATCTTTTGCCAAAAGTGTAATAGGTACCCCCGAGTTCATGGCACCCGAAATGTACGAGGAGCATTACG ACGAGTCGGTTGATGTCTACGCTTTCGGGATGTGCATGCTGGAGATGGCCACCTCAGAGTATCCCTATTC GGAGTGCCAGAACGCCGCCCAGATCTATCGCAAGGTCACCTGTGGCATCAAGCCGGCCAGCTTTGAGAAG GTGCATGATCCTGAGATCAAGGAGATCATCGGAGAGTGCATCTGCAAGAACAAAGAGGAGAGGTACGAGA TTAAGGACCTGCTGAGCCACGCCTTCTTCGCGGAAGACACGGGCGTCCGGGTGGAACTTGCAGAGGAGGA CCATGGCAGGAAGTCTACCATAGCCCTGCGGCTTTGGGTAGAAGACCCCAAGAAGCTGAAGGGCAAGCCC AAAGACAATGGAGCGATAGAGTTTACCTTTGACCTGGAGAAGGAGACCCCCGACGAGGTGGCCCAAGAAA TGATTGATTCCGGATTTTTCCATGAGAGTGATGTGAAGATTGTGGCCAAGTCCATCCGGGACCGGGTAGC ACTCATTCAATGGCGTCGTGAGAGGATCTGGCCTGCACTGCAGTCTCAGGAGCCAAAGGACACAGGCAGC CCTGACAAGGCCAGGGGTCTGCCAGCACCCCTGCAGGTCCAGGTGACCTACCATGCACAGAGCGGGCAGC CTGGACAGCCAGAACCTGAGGAGCCTGAGGCGGACCAGCATCTCCTGCCCCCTACCTTGCCAGCTAGCGT TACCTCCTTAGCCTCGGACAGCACCTTTGACAGTGGCCAGGGCTCTACCGTGTACTCGGACTCCCAGAGC AGCCAGCATAGCATGGTGCTCAGCTCCCTCGTGGACACAGCTCCAACCCCAGCCTCGTGCGTATGCAGCC CCCCTGTGAGTGAGGGGCCCGGCTTGACACACAGCCTGCCTAAACTAGGGGCCTTCCAGCAGCCAGCCGC TGTGCCTGGCCTATCAGTGGGCCCTGTGCCACCCCCTGCCCGCCCTCCACTCCTCCAGCAGCATTTTCCA GAGCCTTCCATGAGCTTCACCCCTGTGCTGCCACCACCCAGCACCCCTGTGCCCACGGGCCCAAGCCAGC CAGCACCCCCAGTTCAGCAGCCTCTTCCAATGGCCCAGCCACCTACCCTGCCGCAGGTCCTGGCCCCACA GCCCATGGGCACTGTCCAGCCAGTGCCCTCTCATTTGCCTCCATACCTGGCTCCAACCTCCCAGGTAGTG GCCCCTGCTCAGCTGAAGCCTCTCCAGATGCCACAGCCACCTCTACAACCTCTTGCTCAAGTCCCTCCGC AGATGCCTCAAATGCCTGTGGTTCCCCCCATCACTCCCTTAGCAGGACTCGACGGCCTCCCTCAGACCCT CGCTGACCTGCCGGCTGCAAACGTGGCCCCTGTGCCGCCACCTCAGTATTTCTCACCTGCTGTGATCTTG CCAAGCCTCACAACGCCCCTGCCCACATCACCGGCCCTGCCTCTGCAGGCTGTCAAGCTGCCTCACCCCC CTGGGGCACCCCTGGCTGTGCCCTGTCAGACCATCGTGCCAAACGCACCAGCTGCTATCCCCCTCTTGGC CGTGGCCCCGCAGGGTGTGGCCGCACTGTCCATCCATCCAGCAGTGGCCCAGATCCCAGCCCAGATCCCA GCCCAGCCTGTGTACCCAGCAGCCTTCCCAGGAATGGTACCTGGAGACGTCCCTCCCTCACCCCACCACA CAGTGCAGAGCTTACGGGCCACCCCTCCACAGCTGGCCTCGTCTGTGCCTCCTCAGTCTGTCCAGCCCAG TGTCGTTCATCTACCTGAGCAGGCTGCTCCATCTGCTGCCTCAGGGACCCAGGTCCTGCTGGGCCACCCT CCTTCGTACACCACGGATGTTGCTGCTCCAGTCTCTGCTGTGTCCCTGCCGCCTGCTGTCCTTTCCCCTC CTCTGCCAGATACTCTGCTGCCTACTGTCCCTGAGCTCCTGCCCAAGCTCCCCAGCTCTCTGGCCCCTAC GGTGGTCGCAGCTTCTCAGAGTGTGCCCACACAGACCTCTACACTCCTCCCACCAACAAACCCACCTCTG CCCACTGGGCCTGCAGTCGCTGGCCCCTGCCCTGCTGTCCAGCTGACGGTGGAAGCAGCTCAAGAGGAGC AGGTCTCACAAGACAAACCCCCGGGCCCCCCACAGAGCTGTGAGAGCTTGGGAGGTTCTGATGTCACCTC TGGAAGAGATCTGAGTGACAACTGTGAAGGCACCTTTGGAACGGGCAGGCTGGAGGGAAGGGCTGCCCGG AAACACCACCGTAGGTCCACTCGTGCACGCTCCCGGCAGGAGAGGGCCAGCCGGCCCCGGCTCACTATCC TGAATGTGTGTAACACGGGGGACAAGATGGTGGAGTGTCAGCTGGAGACGCATAACCACAAGATGGTGAC CTTCAAGTTTGACCTCGATGGGGACGCTCCAGATGAGATTGCCACATACATGGTAGAGCATGACTTCATC CTGCCCGCTGAGCGAGAGACGTTTATCGAGCAGATGAAGGACGTCATGGACAAGGCAGAAGATATGCTCA GTGAGGACACGGATGCTGATCATGGCTCTGATACAGGGACCAGCCCTCCACACCTGGGTACCTGTGGTCT GACCACGGGGGAGGAGAATGGACAGTCCCAAGCCAATGCCCCTGTCTATCAGCAGAATGTCTTGCACACG GGGAAGAGGTGGTTTATCATCTGTCCAGTGGCTGAGCACCCAGCAGCAGACACTTCTGAGTCATCACCCC CACTTCCTCTAAGCTCTTTGCAGCCAGAAGCCAGCCAAGACCCAGCACCCTATCCAGACCAGCTGTCCTT GACGGACAAACCCAGTTTCCCAGCTGCTCAGCAGCTCCTGAGCCAGGCAGGCTCCAGCAACCCTCCTGGT GGAGCATCAGCCCCCACGGCCCCATCTTCCCCTCCTGTGACTTCTGTAATGCCCGCAGCACCAGCCACCA GCACCGTGCCAGAGTCAGCAGCAGGGACTGCCATGCAGGCAGGAGGTCCAGGGACTCATCAAGGACCAGC CAGTGTTCATGAGACCCTCCACCCACTGGCCGAGACACATCGTGCCCAGGGTGCTGCCCAGCCCCTTAGC ACTGGCCAAGGACCTTGCACTCCAGCTCTGGAGGCCTCTCACTGCTCCACAGGTCTGGGAGAGCCCATCT CAACCAAGGAGGTCAGCACTCAAGGAGGGACCCTGTCTGCTTCAATGCCAGAGCCCAGCCCTCCCACTGG GGCTGTACAATCTGTACCGAGTCAGCCACCACCCCCACTGCCCATCACAGTGGGGACTATAGGTCTGGCA GCTCCCCAGCTTCCCAGTCCCCCTCTGGGGCCTACTGCTCCCCCACCACCACCCTCAGCCTTGGAATCTG ATGGGGAAGGGCCACCCCCTAGGGTGGGCTTCGTGGACAACACCATCAAGAGTCTGGATGAGAAACTGCG GACTCTGCTCTACCAGGAGCATGTGCCCACCTCCTCAGCCTCGGCTGGGACCCCCTTGGAGGCAAACGAC AGAGACTTCACCCTGGAGCCCCTTCGAGGAGACCTCCCCTCGGCCTTGAGTGACAAGACCCCAAGCCTCA CCCAGCAGAGCCAGCCCTCATTGGAAAAGTCAGAAACGGCTCCTGCAGGGTGGGCCCTGGCCCCGCGGGA ACAGGGTGCGTCCTCTTCAATGACAGCAGAGTCCTCTTCCAGCAACACACTGGGCTGTGACAGTGACACA GGGCAGGTGGCCTCGGACTCCCCCACAGCACCCAGTGTCCCCCAGGATACCTCCGTTTCTTCCGTCCCTA CGCACATGGATCCCAAAGATCAGAATGGTAGTGCTCCCAGAGAAACCTTGGTAGCACCCATGCAGAGTTG CCCAGGGACCTTCACAGAGGGCAGCCCAGCCCAGCTGCGTGGCGTCCGGGACTCCGGGTCCCCCCATAAG CGGCCAGGGCAGCAGGACAGCAGTTCTCCAGCCAAAACTGTGGGCCGCTTCTCCGTGGTCAGCACACAAG ACGAGTGGACTCTGGCGTCACCTCACAGCCTGCGGTACTCCGCCCCACCTGATGTCTACCTGGATGAGAT CCCCTCTAGCCCTGAGGTGAAGCTGGCAGTTCGTCGAGTACAGACAGCCTCCTCCATCGAGGTTGGCGTT GAGGAGCCTGCCTCCAGTGACTCTGGGGATGAATGCCCAAGAAGGAGGACCCAGGTGCAGAAGCAGTCCT CCCTGCCGGGGAGCGGTGGCGTGGCAAGTGACTTTGTGAAGAAGGCCACTGCCTTCCTGCACAGGTCATC AAGGGCTGGCTCGCTGGGCCCTGAGACACCCAGCAGGGCAGGTGTGAAGGTCCCTACCATCAGTATCACC TCCTTCCACTCCCAGTCATCCTACATCAGCAGTGACAACGACTCGGAGTTTGAGGACGCAGACATAAAGA AGGAACTGCGCAGCCTGCGGGAAAAGCACCTGAAGGAAATCTCTGAGCTACAGAGCCAGCAGAAACAGGA GATTGAAGCCCTGTACAGACGCTTGGGCAAGCCGCTCCCTCCCAATGTGGGGTTCTTCCACACCGCACCC CCTATGGGCCGCAGGAGAAAAACCAGCAAGAGCAAATTGAAGGCGGGCAAGCTGCTGAATCCCCTGGTGC AACAGCTCAAGGTCGTGGCCTCCAGCACAGGGTCAGCCCTGAAGCGTCTCTGCCTAGGCAAAGAACACAG CAGTAGGTCTTCCACCAGCAGCCTGGCCCCAGGCCCTGAGCCAGGTCCCCAGCCCACCCTGCACGTCCAG GCGCAGGTGAACAACAGCAACAACAAGAAGGGCACCTTCACGGATGATCTGCACAAGCTGGTGGATGAGT GGACAACGAAGACAGTGGGGGCGGCCCAAGTGAAGCCCACGCTCAACCAGCTGAAGCAGACGCAGAAGCT GCATGACATGGAGGCCTCTGGAGAGGCTCGGGCTACATCTGTGCCTCGAGGAGGAGTGGCAACATCGTGT CTGGCCCCAGCACCTGGCCCTCTGTCCAGCACAGCCATTCCTGGAGTCACCCCAGCCCTGCCTGTGCCCA CACCAGATCCTGAGAGTGAAAAGCCCGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217733 representing NM_001191556 Red=Cloning site Green=Tags(s) MDGDGGRRDAPGALMEAGRGTGSAGMAEPRARAARLGPQRFLRRSVVESDQEEPPGLEAAETPSAQPPQP LQRRVLLLCKTRRLIAERARGRPAAPAPAAPAAPPGSPSAPSDPGPERAGTQEPSPDPTTASAAATPVPD GGPRQEEAPAPTQEDTGATEAKPEPGRARKDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVY KGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTS GTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKR ASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEK VHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKP KDNGAIEFTFDLEKETPDEVAQEMIDSGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQSQEPKDTGS PDKARGLPAPLQVQVTYHAQSGQPGQPEPEEPEADQHLLPPTLPASVTSLASDSTFDSGQGSTVYSDSQS SQHSMVLSSLVDTAPTPASCVCSPPVSEGPGLTHSLPKLGAFQQPAAVPGLSVGPVPPPARPPLLQQHFP EPSMSFTPVLPPPSTPVPTGPSQPAPPVQQPLPMAQPPTLPQVLAPQPMGTVQPVPSHLPPYLAPTSQVV APAQLKPLQMPQPPLQPLAQVPPQMPQMPVVPPITPLAGLDGLPQTLADLPAANVAPVPPPQYFSPAVIL PSLTTPLPTSPALPLQAVKLPHPPGAPLAVPCQTIVPNAPAAIPLLAVAPQGVAALSIHPAVAQIPAQIP AQPVYPAAFPGMVPGDVPPSPHHTVQSLRATPPQLASSVPPQSVQPSVVHLPEQAAPSAASGTQVLLGHP PSYTTDVAAPVSAVSLPPAVLSPPLPDTLLPTVPELLPKLPSSLAPTVVAASQSVPTQTSTLLPPTNPPL PTGPAVAGPCPAVQLTVEAAQEEQVSQDKPPGPPQSCESLGGSDVTSGRDLSDNCEGTFGTGRLEGRAAR KHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFI LPAERETFIEQMKDVMDKAEDMLSEDTDADHGSDTGTSPPHLGTCGLTTGEENGQSQANAPVYQQNVLHT GKRWFIICPVAEHPAADTSESSPPLPLSSLQPEASQDPAPYPDQLSLTDKPSFPAAQQLLSQAGSSNPPG GASAPTAPSSPPVTSVMPAAPATSTVPESAAGTAMQAGGPGTHQGPASVHETLHPLAETHRAQGAAQPLS TGQGPCTPALEASHCSTGLGEPISTKEVSTQGGTLSASMPEPSPPTGAVQSVPSQPPPPLPITVGTIGLA APQLPSPPLGPTAPPPPPSALESDGEGPPPRVGFVDNTIKSLDEKLRTLLYQEHVPTSSASAGTPLEAND RDFTLEPLRGDLPSALSDKTPSLTQQSQPSLEKSETAPAGWALAPREQGASSSMTAESSSSNTLGCDSDT GQVASDSPTAPSVPQDTSVSSVPTHMDPKDQNGSAPRETLVAPMQSCPGTFTEGSPAQLRGVRDSGSPHK RPGQQDSSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEIPSSPEVKLAVRRVQTASSIEVGV EEPASSDSGDECPRRRTQVQKQSSLPGSGGVASDFVKKATAFLHRSSRAGSLGPETPSRAGVKVPTISIT SFHSQSSYISSDNDSEFEDADIKKELRSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAP PMGRRRKTSKSKLKAGKLLNPLVQQLKVVASSTGSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPTLHVQ AQVNNSNNKKGTFTDDLHKLVDEWTTKTVGAAQVKPTLNQLKQTQKLHDMEASGEARATSVPRGGVATSC LAPAPGPLSSTAIPGVTPALPVPTPDPESEKPD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191556
ORF Size	6189 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191556.1, NP_001178485.1
RefSeq Size	6450 bp
RefSeq ORF	6192 bp
Locus ID	306811
MW	218.3 kDa

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