Naca (NM_001198580) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217748 representing NM_001198580 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGTGAAGCCACAGAAACCGTCCCTGCTACAGAGCAGGAGTTGCCACAGCCTCAGGCTGAGACAG CTGTGCTTCCTATGTCTTCAGCCTTGAATGTTACTGCTGCTCCAGGGCAGCCTGGATCTCCCTCTCCTTC CTCCCTGGGCCCCCAGCAAAGCCCTCTAGTGACTGCTGCCCATCAGTCTTCTCCACTCCCCTCTAGTCTT CCCTCCACCCCCTTTGAAGTTCCTTTTGCCCAGCCAGAAACTGCTGTGCCTTCAGGAGCTGCCCCTCCCG CCCCAACTTTCCTGCCACACCTGATAGGGCCTCCTATCTCCCCAGCTGCCTTAGCTTTGGCCTCTCCCGT GATAGGTCCAGCTCAGAAAGGTGCCCGTTCCTCCTCTGCTCCCTTGTCTCTGGTCGCATTGGCTCCTCAC TCTATTCAGAAGAGCTCTGTGTGTCCCCCTCACCCTCTCACCTTACCTCCATCAGCTGCTGGAGCAGAGT CAGGGGCATTGACATCTCTGACAGCTTCCATCCCTCTCTTAGAATCAAAGGCTTCTACCAGTCAAGTTCC CTCTCAGGGAACCCCGAATCTGAAAGGTACTACTACCCCGTGTCCTCCAGATGTAGTCAGTGCTTTTCCT TCCCACCTTGAAAACCCCCTGGCCTCTGTTCAGCCTGGTTTAACATCCTGTCCTCAAACACTGTCCATCA CTTCCCCAGTTAAAGGTGTTCCCATTTCCTCAGCTCTGACACAAAGTCTGCTAAGCCTTAACCTTAGGGG ACCTGTTAGTTCACCTCCTCAGAGCACTGTAACTCCCAGCATCCCCCCAGCAGCCTCAGCTTCCCTGGGT TCTCCTCTTCCACTTCTACATCACAGTTCTTTGGATTCTCCTGTCCAGCCTCCAGGTCAGTCAGGTCTTA CAGTGAACACCACCATTTCTGTAGATCATCCTTTTGTTGCAGCCTCTTACCCACCTGAGCGATCGGCAGT TTCTCCTCTTCCTTCCAGACATGAGCTGGCTGCTGATTCTGGGGCAGCTCCATCTATTGACAAAGGCTCC TCAGCTGTTACTAAGGAAACCTGCAGCTCTTCTGGCTCTGCGAATGAAGCTGGTACTTCATTGTCTCCTG CAGCCCCTCTCACTCCCAAAAGTTCTAATGCTGCTCTGCAGCCCTCGGTGACTCAGGTTCCTCCTCCTCA AAAACCTGGCTTAAAAGAAACTCCTGCCTCTTGTATTAGAGCCATGCACCCTGCACTGGATAACCCTTCT ACCATGTCTGTAGCACCTGCCACTCATGTGTCTCCTCCAGCTTCATCGGGTCTTATAAAAAGTGAAGACC CAGCGTCCCCTGTTTCTTCACTTTCAGTACCTGCTGCTCATAAGCCGTTTCCTGCCCCTTCAATGTCAAC TACTCTAGGGATACCTGTGTCTCCTCTGCCAGCCACTGAAGGCCTTCAAAATCTTCCGATTTCAGTAAAT GCAGGAGCCCCTGTTTCTCCAGCCCAGGCAGGGCTTCCTATCAGAAAAGATACTACTGTACAAACCTTGG CCCCAGTAGCACTTAAAGAGTCTCCCTCTTCTCAAAGCGCATCTTCTTTGGAGGTGCTTTCAGAAGACAC AAAGAAGACTACAGGAGGGCCTGCCCCTGTGGTGCGACCAGCCATTGCTACTGGTGCTCCTACTACCCTG GACTACCCACCTGCCGGCTATGTGAACCCAAACCCTGTCAGTCAGCCTCTCAAAAGTTCTGCCCCAGCAA TGGCTCCATTTCTTTCAGAAAGTTGTGATTCTAAAGGGCTGGTCCCTAGAACTGCCAAAGATACCTTTCC TTCTGTTAATTCACCTCTGGTTCCTTTACCCTCTGAAGGCTGTCCTGTGGCTCCATCTATGGCGTTGTCC CACCAGAATGCTTCTGTTTCTGTGACTACTGCCAAGTCTGTACCCTTTCCTGCTCCTCCCTCAGCTGGAG TTTCTTTTTCCAGTGCAAAGAAAGTCGATGTTGTCTCTCAGATGGAGTCTTCAGACTCCTCTCAAGAAGG GCACCCAAATGCTTCTGTGTCTTCTCAGACTAAACGGCCTGCATCCAAAAAGGGTTCTTTTCCCTCAGAT GTTTCTCCTGGAAATGTTTCCTCTCCTCATTGTTCACTTGAGACTTCTTTTCTCCCAGAGGCAGGTCTTT CTTTTCAAGGCCCTAAAGGCGCCTCTATTGTTCCCACAGAAACTTCTGTATCCTCCAAAAAGACCCCAGC AGAAATTCTTCCTTCCCCCCAAAAGACACCAACGGCTACAGCTTCCAGACTGACCTCAGCAGTCCAGTCT CCTAAACTGGACCCTACTGTATCAGATGTGATTCCAGCAACTGTAGTCCCCAGAGATACTTCTGCAAGCC TTTCTCTCAAAAGTGTCCCAGCTGTGACTTCTCTGTCTTCCCAAAAGGCCCCTGCAGCCCCATCTCCTAA CGAAGCCCTCGTTGCTCCTACAGAGATTCCCACCTCCCTCAAAAATGCCCTAGCAACTACAGCCCCGAAA GAGACTTTAGCAACCTCAACTCTCAAAGTTGCCTCTACTGCCCCAGTTGTGACTTCTCCCTCCCCACAGA AGACTCCAAAATCCGTATCTCCTAAGGGTGCCCCCGCTGTCACCTCTAAAAAGGCAAAAGAAATTGCAGC CTCCAAAGAAGTGTCAACATCCCAGTTTCCCAAAGAGGCCCCTCTTCTACCACATGCAGCTCCTACCTCC CCACCAAAGTCCCCAGCCACTGACCCACTCAATGGGGCTCTAACAAGCCCATCCCCCAAAGTTGCCCCTG CCACCCTAGTTGAGAGTCCTACCTCTCCTAAAAAGGCCCCCAAAACTGCAGCCCCCAAAGAAACTCCAGT GACTCCATCTCTTGAAGGGGTTACAGCTGTACCACTGGAGATTAATCCTTGCCCCAAAAAGGCCCCCAAA ACTTCAACCCCCAAAGAAAGTTCAGTGACTCTATCTTCCAAAAGAACCCCTAAAAGTGCAGTTTCTAAAG AAATTCCTTCTAACGAAGTCACAGCTGTGCCATTGGAGATTTCTCTGCCTCTAAAAGAGACCTCCAAAAG TGCAATACCTGTTGAAAAGTCAACCTCATCCTCAAAAAGTTCCCCCAAAACTGTAGCCCCCAAAGAAACT CTGCCTGAAGAGGTCACAGCTGTGCCTCTGGAGGTTTCTCTGCCTCCAAAAGAGATGCCCCAAAATGCAA GCCCCAGAGAAACTCCACCTGAAGGAGTCACAGCTGTGCCATTGGAGATTCCTCCTACCCCAAAAAAGGC CCCCAAAACTTCAGTCCCTAAACAGATTCCTGTAACCCCATCTCCCAAAGATGCCTTTACTACCCTAGCT GAAAGTCCTCCCTCCCCCAAAAAGGCACCCACAACTGCAGCCCCCAAAGAAGTTCCAGCAGCCCCATCAG TGGGGATCACAGCTGTATCAGAGATTTCTCCCTCCCCAAAAAAGACCTCAAAAACTGCAGTCCCCAAAGA AGATTCAGCAACCTCATACCCCAAAAGGTCCCCCAAAACTGCAACTCCCAAAGAAAATTCAACTTCATCC CCAAAAAGGTCCCCCAAAATTGAAGCCCCCAAAGAAACTCTAGCCACATCTCCTGAGGGGGTTACAGCTA TGCCACTGGAGATTCCTCCTTCCCCCGCAGCTCCAGCCTCCAAACGGGTTCCTGTGACCCCATCTCCCAA AGGTGCCTCTAATACCCTAGCTGAGAGTTCTGCCTCCCCTAAAAAAGCCCCTAAAACTGCAGCCCCCAAA GAAACCTCAACTCCATCCCCCCAAAAAATTCCTAAAATGTCAGGTCCCAAAGAGACTCCAGCAACCCCAC CTTCCAAAAAGAGCCCCAAAACTGTAGCCCCCAAAGAAACTTCAGCACCTTCTGAGGGGGTCACAGCTTT ACCACTGAAGGTTCCTCCCTCCCCCAGAAAGGCCCCCAAAACTGCAGTCCCCAAAGAAACTCCAGCCCCA TCTCCTGAAGGGGTCACCACTGCACTAGTGCAGATTCCTCCCTCCCCCAGGAAGGTCAAGAGAACAAGCT CCAAAGAAACACCCACAAACCCATCTCCTGAAGGAGTTACAGCTGCACCACTAGAAATTCCCTTAACTTC CAAAAAGAACCCCAAAATGGCAAGCCCCAAAGAAATGCTAGTAACCCCACCTTCCAAAAAGGTTTCCCAA ACTGGAGATCCCAAAGAAACTCCACCTGAAGGGGTCACAGCTGTGCCACTGGAGATTCTTCCTTCCCCCA AAAAGACTCCCAAAACTGTAGTCCCTAAACAGGTTCCTGTAACCCCATCTCCTAAAGATGCCCCTACTAC TCTAGCAGAAAGTCCTCCCTCCCCCAAAAAGGCACCCACAACTGCAGCCCCACCCTCTGAAAGGGTCACC ACTGCACCACCAGAGAAGCCTGCCACCCCACAGAAGGCCTCAGCGACCGTAGCCTCCAAAGTGCTGGTCC CTGCTGAGACACTGGAAGTTGCTGTCTCCCCCAAAGAGACTCATCCCTCCTCCCATAAAAAGACCCCAAA GACTGTAGATCTTAAAGAAGGTCCAGCAGCCCTCCCTCCCTCCCCCACAAAGAGCCCCAAAATTTCATCT TCCAAAAAGGCACAAAATACTTCTGCCCCTATCGAGTTTCCAGCAAGCTCTTCTATTAAAGCTGTCACCA CCTCACTGGCACAGACGGTTCCCTGTGTGCAAAAGGCTCCATCAACAATAGCCCCCCAGGAGAATTCAGC TGCCCCAGTTGCTATTCCTGTCTCCAACAAAAGCCCAGCAGCCCCAACTACTGCAAATGCTTCTGTTGCC CTAGCTGCCGCTTCCCTTTCCCCAACTCCCCAAGTCAAGATTTCTCCTTCCAAAAAGACCCCAGCTACTT CAGCTCTTCAAACAGTTCCAAAAGAGGCCACAACCATCCCATCTGGTAAAAAGGCTCCAGCCATTGAGAC CTCCAATGAGACTTCAACAACTCTATCTCTCAAAGGGGCCCCCAAGGAGACCTCAGAAACTTCAACATCC AAAGGAGTTCTCACGTCCACAGTCAACTCTTCCTCTCCTAAAAAGGTCCCATCCTCCAAAAGGGCCTCTA CTCTCCCAGCTACAACTCTTCCCTCTATTAAAGAGGCCTCTGTTCTCTCACCCGTTCCTACTTCTTCCAC AAAAGACTCCCACACTTACCCAGTGTCCACAGCTTGTAGCACAGCAACTACTACCCCTCAGGCATCTGAA AAGCTCCCAGCAAAGAAAGAACCTGCAGCTCTCACAGAAGTGCCTGCTGCACCTGCTCCAGAAAGTGCTT TGGCCATCACTGTTCCCATTCAGAAAGTCCCAAGAACCAAGCGCAATTCTGCTTCGCCTCCTAAGTGCCC AGATCCCTCTGCTAAGAAAGATACAAAGGGCCTCCTTTCTGCGGTAGCTCTAGCCCCTCAGACAGTCCCT GTTGAGAAAAACTCTTCAAAACCTATAGAAGTTCTGCCTCTTTGCCCTGCAAAAGGCAGCGATTCTGTTC ATTCCCCAAAGGGACCCATGGAGTCTCAGGAAGCTACTCCTCAGGCAGCATTTACCTCTGACAAAGTCCT TCCAAAAGCTGTGTCTAAGTCTGTGGCTCCAAAGCCTACCCTAGCAGCTTCCCTGACTCTTGCTCCCTCC CCAGTTCCCCCTCTGCCTCCTAAGCAGCCATTTCTTCAATCGGCACCTGAGTCCGTGCTGGAATCACCCT CTAAGCTCCCAGTCCCTGCAGATGAGGATGAGCTGCCGCCTCTGATTCCCCCGGAAGCAGTCTCTGGGGG AGTGCCTTTCCAGCCGATCCTGGTCAACATGCCCACCCCTAAACCTGCTGGGATCCCTGCCCCAGCCCCC TCTGCCAAGCAGCCTGTTCTGAAGAACAACAAGGGGTCGGGAACAGAGTCTGACAGTGATGAATCAGTGC CAGAACTCGAGGAACAGGATTCCACACAGACAGCCACACAACAGGCCCAGCTGGCAGCCGCGGCTGAGAT CGATGAAGAACCTGTTAGTAAAGCCAAGCAGAGTCGGAGTGAGAAAAAGGCAAGGAAGGCCATGTCCAAA CTGGGTCTTCGACAGGTTACAGGGGTTACGAGAGTCACTATCCGGAAATCTAAAAATATCCTCTTTGTCA TCACAAAACCGGATGTCTACAAGAGCCCAGCTTCAGATACCTACATAGTGTTTGGGGAGGCCAAGATTGA GGACTTGTCGCAGCAAGCACAGTTGGCAGCTGCTGAGAAGTTCAAAGTTCAAGGAGAGGCTGTTTCCAAC ATTCAGGAGAACACGCAGACGCCGACTGTCCAAGAGGAGAGCGAAGAAGAGGAGGTTGATGAAACGGGTG TGGAAGTTAAGGACATAGAGTTGGTCATGTCACAAGCAAATGTGTCAAGAGCAAAGGCTGTCCGAGCCCT GAAAAACAACAGTAACGATATTGTAAATGCTATTATGGAATTAACAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217748 representing NM_001198580 Red=Cloning site Green=Tags(s) MPGEATETVPATEQELPQPQAETAVLPMSSALNVTAAPGQPGSPSPSSLGPQQSPLVTAAHQSSPLPSSL PSTPFEVPFAQPETAVPSGAAPPAPTFLPHLIGPPISPAALALASPVIGPAQKGARSSSAPLSLVALAPH SIQKSSVCPPHPLTLPPSAAGAESGALTSLTASIPLLESKASTSQVPSQGTPNLKGTTTPCPPDVVSAFP SHLENPLASVQPGLTSCPQTLSITSPVKGVPISSALTQSLLSLNLRGPVSSPPQSTVTPSIPPAASASLG SPLPLLHHSSLDSPVQPPGQSGLTVNTTISVDHPFVAASYPPERSAVSPLPSRHELAADSGAAPSIDKGS SAVTKETCSSSGSANEAGTSLSPAAPLTPKSSNAALQPSVTQVPPPQKPGLKETPASCIRAMHPALDNPS TMSVAPATHVSPPASSGLIKSEDPASPVSSLSVPAAHKPFPAPSMSTTLGIPVSPLPATEGLQNLPISVN AGAPVSPAQAGLPIRKDTTVQTLAPVALKESPSSQSASSLEVLSEDTKKTTGGPAPVVRPAIATGAPTTL DYPPAGYVNPNPVSQPLKSSAPAMAPFLSESCDSKGLVPRTAKDTFPSVNSPLVPLPSEGCPVAPSMALS HQNASVSVTTAKSVPFPAPPSAGVSFSSAKKVDVVSQMESSDSSQEGHPNASVSSQTKRPASKKGSFPSD VSPGNVSSPHCSLETSFLPEAGLSFQGPKGASIVPTETSVSSKKTPAEILPSPQKTPTATASRLTSAVQS PKLDPTVSDVIPATVVPRDTSASLSLKSVPAVTSLSSQKAPAAPSPNEALVAPTEIPTSLKNALATTAPK ETLATSTLKVASTAPVVTSPSPQKTPKSVSPKGAPAVTSKKAKEIAASKEVSTSQFPKEAPLLPHAAPTS PPKSPATDPLNGALTSPSPKVAPATLVESPTSPKKAPKTAAPKETPVTPSLEGVTAVPLEINPCPKKAPK TSTPKESSVTLSSKRTPKSAVSKEIPSNEVTAVPLEISLPLKETSKSAIPVEKSTSSSKSSPKTVAPKET LPEEVTAVPLEVSLPPKEMPQNASPRETPPEGVTAVPLEIPPTPKKAPKTSVPKQIPVTPSPKDAFTTLA ESPPSPKKAPTTAAPKEVPAAPSVGITAVSEISPSPKKTSKTAVPKEDSATSYPKRSPKTATPKENSTSS PKRSPKIEAPKETLATSPEGVTAMPLEIPPSPAAPASKRVPVTPSPKGASNTLAESSASPKKAPKTAAPK ETSTPSPQKIPKMSGPKETPATPPSKKSPKTVAPKETSAPSEGVTALPLKVPPSPRKAPKTAVPKETPAP SPEGVTTALVQIPPSPRKVKRTSSKETPTNPSPEGVTAAPLEIPLTSKKNPKMASPKEMLVTPPSKKVSQ TGDPKETPPEGVTAVPLEILPSPKKTPKTVVPKQVPVTPSPKDAPTTLAESPPSPKKAPTTAAPPSERVT TAPPEKPATPQKASATVASKVLVPAETLEVAVSPKETHPSSHKKTPKTVDLKEGPAALPPSPTKSPKISS SKKAQNTSAPIEFPASSSIKAVTTSLAQTVPCVQKAPSTIAPQENSAAPVAIPVSNKSPAAPTTANASVA LAAASLSPTPQVKISPSKKTPATSALQTVPKEATTIPSGKKAPAIETSNETSTTLSLKGAPKETSETSTS KGVLTSTVNSSSPKKVPSSKRASTLPATTLPSIKEASVLSPVPTSSTKDSHTYPVSTACSTATTTPQASE KLPAKKEPAALTEVPAAPAPESALAITVPIQKVPRTKRNSASPPKCPDPSAKKDTKGLLSAVALAPQTVP VEKNSSKPIEVLPLCPAKGSDSVHSPKGPMESQEATPQAAFTSDKVLPKAVSKSVAPKPTLAASLTLAPS PVPPLPPKQPFLQSAPESVLESPSKLPVPADEDELPPLIPPEAVSGGVPFQPILVNMPTPKPAGIPAPAP SAKQPVLKNNKGSGTESDSDESVPELEEQDSTQTATQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSK LGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSN IQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198580
ORF Size	6489 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198580.1, NP_001185509.1
RefSeq Size	6627 bp
RefSeq ORF	6492 bp
Locus ID	288770
MW	220.2 kDa

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